Immunological detection of Bacteroides fragilis in clinical samples.

abstract：:Sorbarod biofilms were investigated for their suitability in establishing continuous culture biofilms for the study of bacterial vaginosis. Two important organisms in the condition, Gardnerella vaginalis and Lactobacillus acidophilus, were studied. In contrast to growth in broth culture, both organisms were maintained...

journal_title：Journal of medical microbiology

authors： Muli FW,Struthers JK

更新日期：1998-05-01 00:00:00

abstract：:The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, th...

journal_title：Journal of medical microbiology

authors： Cerqueira GM,McBride AJA,Picardeau M,Ribeiro SG,Moreira ÂN,Morel V,Reis MG,Ko AI,Dellagostin OA

更新日期：2009-09-01 00:00:00

abstract：:Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in Pakistan and is emerging in the community. This is one of the first reports of the prevalent genotypes of MRSA in both hospital and community settings in Pakistan. Isolates collected in 2006-2007 were characterized by PFGE, staphylococ...

journal_title：Journal of medical microbiology

authors： Zafar A,Stone M,Ibrahim S,Parveen Z,Hasan Z,Khan E,Hasan R,Wain J,Bamford K

更新日期：2011-01-01 00:00:00

abstract：PURPOSE:Sporothrix brasiliensis, a member of the Sporothrix schenckii complex, is a major cause of epidemic outbreaks of sporotrichosis due to its greater virulence and ability to evade the immune system. The absence of studies about this species led to this study, with the aim to evaluate the importance of Toll-like r...

journal_title：Journal of medical microbiology

authors： Rossato L,Silvana Dos Santos S,Ferreira LG,Rogério de Almeida S

更新日期：2019-01-01 00:00:00

abstract：:The agglutination of 218 clinical isolates and three ATCC type strains of "Streptococcus milleri" was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains...

journal_title：Journal of medical microbiology

authors： Kellens JT,Jacobs JA,Peumans WJ,Stobberingh EE

更新日期：1994-07-01 00:00:00

abstract：PURPOSE:Membrane fluidity to a large extent is governed by the presence of branched-chain fatty acids (BCFAs). Branched-chain α-keto acid dehydrogenase (BKD) is the key enzyme in BCFA synthesis. A Staphylococcus aureus BKD-deficient strain still produced substantial levels of BCFAs. Pyruvate dehydrogenase (PDH) with st...

journal_title：Journal of medical microbiology

authors： Singh VK,Sirobhushanam S,Ring RP,Singh S,Gatto C,Wilkinson BJ

更新日期：2018-04-01 00:00:00

abstract：:The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be 30 % to 40 % of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of...

journal_title：Journal of medical microbiology

authors： Noordin A,Sapri HF,Mohamad Sani NA,Leong SK,Tan XE,Tan TL,Mohamad Zin N,Neoh HM,Hussin S

更新日期：2016-12-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an important agent of diarrhoeal diseases worldwide. The role of EAEC virulence factors in the clinical outcome of infection is not completely defined. This case-control study investigated the prevalence of EAEC, its virulence genes and the antimicrobial resistance profile ...

journal_title：Journal of medical microbiology

authors： Bafandeh S,Haghi F,Zeighami H

更新日期：2015-05-01 00:00:00

abstract：:A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated...

journal_title：Journal of medical microbiology

authors： Pourshafie MR,Grimont F,Saifi M,Grimont PAD

更新日期：2000-12-01 00:00:00

abstract：:Rhodococcus equi is an opportunistic human pathogen associated with immunosuppressed people. While the interaction of R. equi with macrophages has been comprehensively studied, little is known about its interactions with non-phagocytic cells. Here, we characterized the entry process of this bacterium into human lung e...

journal_title：Journal of medical microbiology

authors： Remuzgo-Martínez S,Pilares-Ortega L,Álvarez-Rodríguez L,Aranzamendi-Zaldunbide M,Padilla D,Icardo JM,Ramos-Vivas J

更新日期：2013-08-01 00:00:00

abstract：:Simulated throat swabs were prepared with known numbers of Streptococcus pyogenes; some were suspended in pasteurised human saliva and an equal number in saline. Two types of commercially available swabs were tested; these were composed of (1) plain, buffered, cotton wool, and (2) albumen-coated cotton wool. The mean ...

journal_title：Journal of medical microbiology

authors： Ross PW

更新日期：1977-02-01 00:00:00

abstract：:Immunodiffusion analysis of Mycobacterium lepraemurium indicated the presence of at lease six antigens. Comparative analysis of the M. lepraemurium antigen-antibody system with similar systems established for other mycobacterial species, showed that M. lepraemurium shared up to two antigens with other species. Althoug...

journal_title：Journal of medical microbiology

authors： Navalkar RG,Dalvi RR,Patel PJ

更新日期：1975-02-01 00:00:00

abstract：:Bacteraemia in children is a potentially life-threatening condition. The objective of this study was to determine trends in the aetiology of bacteraemia in children aged 1 month-15 years in England and Wales by collecting data voluntarily reported by National Health Service hospital microbiology laboratories. Over the...

journal_title：Journal of medical microbiology

authors： Henderson KL,Johnson AP,Muller-Pebody B,Charlett A,Gilbert R,Sharland M

更新日期：2010-02-01 00:00:00

abstract：:Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...

journal_title：Journal of medical microbiology

authors： Poddar SK,Sawyer MH,Connor JD

更新日期：1998-12-01 00:00:00

abstract：:PCR has proved superior to conventional blood culture for diagnosing bacteraemia in the presence of antibiotics. Nevertheless, even PCR might yield false-negative results if the template DNA were to be cleaved by serum DNAases after antibiotics had induced bacterial death. To evaluate the cleavage of bacterial templat...

journal_title：Journal of medical microbiology

authors： Heininger A,Ulrich M,Priebe G,Unertl K,Müller-Schauenburg A,Botzenhart K,Döring G

更新日期：2001-03-01 00:00:00

abstract：:Helicobacter pylori is classified as a class I carcinogenic factor and its persistent colonization in the stomach induces gastric cancer. Cancerous Inhibitor of PP2A (CIP2A) is a newly identified oncoprotein overexpressed in gastric cancer. Serving as a key oncoprotein, CIP2A also participates in regulation of senesce...

journal_title：Journal of medical microbiology

authors： Zhao D,Liu Z,Ding J,Li W,Sun Y,Yu H,Zhou Y,Zeng J,Chen C,Jia J

更新日期：2010-03-01 00:00:00

abstract：:Chickens were immunised with a preparation of purified 14-kDa fimbriae of Salmonella serotype Enteritidis (SEF 14) to raise egg-yolk antibodies for protection trials in mice against subsequent challenge-exposure with the homologous strain of Enteritidis. A pronounced specificity of egg-yolk antibodies against the 14-k...

journal_title：Journal of medical microbiology

authors： Peralta RC,Yokoyama H,Ikemori Y,Kuroki M,Kodama Y

更新日期：1994-07-01 00:00:00

abstract：:Resistance to desiccation and to skin fatty acids was measured in three groups of methicillin-resistant Staphylococcus aureus (MRSA) strains and a group of control strains. Organisms from a large outbreak on a special care baby unit (SCBU), where MRSA had been isolated from staff hands but not from the environment, we...

journal_title：Journal of medical microbiology

authors： Farrington M,Brenwald N,Haines D,Walpole E

更新日期：1992-01-01 00:00:00

abstract：:Eleven strains of Mycobacterium tuberculosis and 13 other species of mycobacteria were examined for susceptibility to 26 strains of viridans streptococci. Susceptibility was detected by the simultaneous antagonism technique, but not by tests of deferred antagonism. It is concluded that in-vitro inhibition of mycobacte...

journal_title：Journal of medical microbiology

authors： Allen BW

更新日期：1985-04-01 00:00:00

abstract：:This study used a previously described multiplex PCR-based reverse line blot (mPCR/RLB) assay to assess the prevalence and distribution of 14 urogenital pathogens or putative pathogens, namely Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, Gardnerella va...

journal_title：Journal of medical microbiology

authors： McKechnie ML,Hillman RJ,Jones R,Lowe PC,Couldwell DL,Davies SC,King F,Kong F,Gilbert GL

更新日期：2011-07-01 00:00:00

abstract：:Polyacrylamide-agarose gel electrophoresis was used to study polymorphism of lactate dehydrogenase (LDH), nucleoside phosphorylase (NSP), phosphoglucose isomerase (PGI), hydroxybutyrate dehydrogenase (HBD), adenylate kinase (ADK) and esterases of 44 strains of Streptococcus pyogenes, 25 group G streptococcal strains, ...

journal_title：Journal of medical microbiology

authors： Bert F,Picard B,Lambert-Zechovsky N,Goullet P

更新日期：1995-06-01 00:00:00

abstract：:A simple method is described for testing milk specimens from nursing mothers for the presence of RNA-dependent DNA-polymerase activity. Positive results were obtained in five of 137 women (3.6%) without a family history of breast cancer, and in six of 31 women (19.3%) with a family history of breast cancer. ...

journal_title：Journal of medical microbiology

authors： McFarlane ES,Ryan SM,Mann E

更新日期：1975-08-01 00:00:00

abstract：:Biofilms are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. Microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. We aimed to screen and characterize biofilm formation by different isolates of Candida on ...

journal_title：Journal of medical microbiology

authors： Zahran KM,Agban MN,Ahmed SH,Hassan EA,Sabet MA

更新日期：2015-04-01 00:00:00

abstract：:Purpose. Antimicrobial susceptibility is slow to determine, taking several days to fully impact treatment. This proof-of-concept study assessed the feasibility of using machine-learning techniques for analysis of data produced by the flow cytometer-assisted antimicrobial susceptibility test (FAST) method we developed....

journal_title：Journal of medical microbiology

authors： Inglis TJJ,Paton TF,Kopczyk MK,Mulroney KT,Carson CF

更新日期：2020-05-01 00:00:00

abstract：:A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...

journal_title：Journal of medical microbiology

authors： Batchelor BI

更新日期：1995-07-01 00:00:00

abstract：:Administration of oestradiol to three strains of female mice induced the oestrous phase of the reproductive cycle in which there are few or no polymorphonuclear leucocytes (PMNL) in vaginal smears. This treatment rendered the mice susceptible to genital tract colonisation by serotype 8 of Ureaplasma urealyticum, inocu...

journal_title：Journal of medical microbiology

authors： Furr PM,Taylor-Robinson D

更新日期：1989-06-01 00:00:00

abstract：:Multidrug-resistant organisms (MDROs) pose significant infection-control challenges in settings with high prevalence and limited isolation facilities. This observational study in an 800-bed hospital determined the prevalence, bacterial density and genetic relatedness of MDROs isolated from ward surfaces, medical devic...

journal_title：Journal of medical microbiology

authors： Tan TY,Tan JSM,Tay H,Chua GH,Ng LSY,Syahidah N

更新日期：2013-05-01 00:00:00

abstract：:In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the i...

journal_title：Journal of medical microbiology

authors： Todkill D,Pudney R,Terrell A,Tuck J,Hutley E,Dallman TJ,Jenkins C,Manuel R,Wade M,Puleston R,Hawker J

更新日期：2018-11-01 00:00:00

abstract：:The aim of this study was to conduct a comparative analysis of the 16S rRNA gene fragment nucleotide sequences for Anaplasma phagocytophilum strains detected in the blood of horses from various parts of Europe. The study comprised 234 horses that had had contact with ticks. Using PCR, the genetic material of A. phagoc...

journal_title：Journal of medical microbiology

authors： Dzięgiel B,Adaszek L,Winiarczyk M,García-Bocanegra I,Carbonero A,Dębiak P,Winiarczyk S

更新日期：2013-12-01 00:00:00

abstract：:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

journal_title：Journal of medical microbiology

authors： Roberts AP,Phillips R

更新日期：1981-05-01 00:00:00