Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism.

Abstract:

:The obligate intracellular pathogen Anaplasma phagocytophilum is transmitted by ticks and causes human granulocytic anaplasmosis, tick-borne fever of ruminants, and equine and canine granulocytic anaplasmosis. In a previous study, the perilipin (PLIN) gene was identified as one of the genes differentially expressed in human promyelocytic HL-60 cells in response to infection with A. phagocytophilum. PLIN is a major adipocyte lipid droplet-associated phosphoprotein that plays a central role in lipolysis and cholesterol synthesis. Host cholesterol and other lipids are required by A. phagocytophilum for infection and multiplication in human cells. In this study, it was hypothesized that PLIN may be involved in infection of human HL-60 cells by A. phagocytophilum. To test this hypothesis, a combination of real-time RT-PCR, immunofluorescence and RNA interference was used to study the expression of PLIN. The results of these studies demonstrated that A. phagocytophilum modulates lipid metabolism by increasing PLIN mRNA levels and facilitates infection of HL-60 cells. The results of these studies expand our knowledge of the role of lipid metabolism in A. phagocytophilum infection and multiplication in HL-60 cells and suggest a mechanism by which A. phagocytophilum modulates lipid metabolism.

journal_name

J Med Microbiol

authors

Manzano-Roman R,Almazán C,Naranjo V,Bloui EF,Kocan KM,de la Fuente J

doi

10.1099/jmm.0.47504-0

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

159-163

issue

Pt 2

eissn

0022-2615

issn

1473-5644

journal_volume

57

pub_type

杂志文章
  • Molecular analysis and species diversity of Nocardia in the hospital environment in a developing country, a potential health hazard.

    abstract:PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia specie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000436

    authors: Rahdar HA,Azadi D,Shojaei H,Daei-Naser A

    更新日期:2017-03-01 00:00:00

  • Moraxella (Branhamella) catarrhalis--clinical and molecular aspects of a rediscovered pathogen.

    abstract::Since its discovery at the end of the nineteenth century, Moraxella (Branhamella) catarrhalis has undergone several changes of nomenclature and periodic changes in its perceived status as either a commensal or a pathogen. Molecular analysis based on DNA hybridisation or 16S rDNA sequence comparisons has established it...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/00222615-46-5-360

    authors: Enright MC,McKenzie H

    更新日期:1997-05-01 00:00:00

  • Identification and evaluation of LPS antigen for serodiagnosis of uveitis associated with leptospirosis.

    abstract::Leptospirosis is a widespread zoonotic disease that affects all mammals in different parts of the world. Though there are many commercial kits available for the diagnosis of systemic leptospirosis, the nature of the antigen has not been described. Therefore, identification of a specific antigen is important. Since ocu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05120-0

    authors: Priya CG,Bhavani K,Rathinam SR,Muthukkaruppan VR

    更新日期:2003-08-01 00:00:00

  • Histoplasma capsulatum in planktonic and biofilm forms: in vitro susceptibility to amphotericin B, itraconazole and farnesol.

    abstract::It is believed that most microbial infections are caused by pathogens organized in biofilms. Recently, it was shown that the dimorphic fungus Histoplasma capsulatum, estimated to be the most common cause of fungal respiratory diseases, is also able to form biofilm. Although the antifungal therapy commonly used is effe...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000030

    authors: Brilhante RSN,de Lima RAC,Marques FJF,Silva NF,Caetano ÉP,Castelo-Branco DSCM,Bandeira TJPG,Moreira JLB,Cordeiro RA,Monteiro AJ,Camargo ZP,Sidrim JJC,Rocha MFG

    更新日期:2015-04-01 00:00:00

  • Observations on the growth and movement of Acinetobacter on semi-solid media.

    abstract::The growth of 29 strains of Acinetobacter spp. on semi-solid media was studied; 19 showed surface swarming and 14 produced channels ("ditches") in the agar that do not seem to have been described previously. An attempt was made to define the cultural and physical conditions for the demonstration of these phenomena. Po...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-3-443

    authors: Barker J,Maxted H

    更新日期:1975-08-01 00:00:00

  • Gas-lipuid chromatographic analysis of metabolic products in the identification of bacteroidaceae of clinical interest.

    abstract::The acid end-products of 185 isolates from the family Bacteroidaceae were separated and analysed by gas-liquid chromatography on broth cultures. Different media were evaluated and definitive studies were performed in a fully supplemented complex medium. The limitations of this approach to the identification of a wide ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-2-81

    authors: Deacon AG,Duerden BI,Holbrook WP

    更新日期:1978-05-01 00:00:00

  • Molecular analysis of Streptococcus pneumoniae clones causing invasive disease in children in Singapore.

    abstract::Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However, there are few published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD are essential to inform policy regarding pneumococcal conj...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.030007-0

    authors: Jefferies JMC,Tee WSN,Clarke SC

    更新日期:2011-06-01 00:00:00

  • Development of a routine laboratory direct detection system of staphylococcal enterotoxin genes.

    abstract::A novel direct detection system has been developed for eight staphylococcal enterotoxin (SE)-encoding genes (sea, seb, sec, sed, see, seg, seh and sei) in milk. Specific detection by real-time PCR was successful for all SE-encoding genes in the reference strains. Furthermore, a novel DNA-preparation method with good r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46027-0

    authors: Nakayama A,Okayama A,Hashida M,Yamamoto Y,Takebe H,Ohnaka T,Tanaka T,Imai S

    更新日期:2006-03-01 00:00:00

  • Competition for glucose between Candida albicans and oral bacteria grown in mixed culture in a chemostat.

    abstract::The competition for glucose as a growth-limiting substrate between Candida albicans and a mixed community of oral bacteria was investigated. A chemostat was operated under glucose-limiting and glucose excess conditions at a dilution rate of 0.05/h. A mixed population of oral bacteria was established and after a steady...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-11-969

    authors: Basson NJ

    更新日期:2000-11-01 00:00:00

  • Prevalence of resistant Helicobacter pylori isolates in Bulgarian children.

    abstract::The aim of this study was to assess the primary and combined resistances of Helicobacter pylori isolates obtained from paediatric patients in 2000-2001 to seven antimicrobial agents. Resistance rates of pre-treatment isolates from 115 children were investigated by the limited agar dilution method alone and by the E-te...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-9-786

    authors: Boyanova L,Koumanova R,Gergova G,Popova M,Mitov I,Kovacheva Y,Derejian S,Katsarov N,Nikolov R,Krastev Z

    更新日期:2002-09-01 00:00:00

  • Impedance monitoring of bacterial activity.

    abstract::Bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. The signal is expressed automatically as a curve similar to grwoth curves produced by other methods. The technique offers a new, rapid and sensitive means of detecting active micro-organisms and i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-1-19

    authors: Ur A,Brown DF

    更新日期:1975-02-01 00:00:00

  • Agglutination of "Streptococcus milleri" by lectins.

    abstract::The agglutination of 218 clinical isolates and three ATCC type strains of "Streptococcus milleri" was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-1-14

    authors: Kellens JT,Jacobs JA,Peumans WJ,Stobberingh EE

    更新日期:1994-07-01 00:00:00

  • Correlation of cefpodoxime susceptibility with cephalothin and cefuroxime for urinary tract isolates.

    abstract::This study attempted to determine whether cefuroxime was superior to cephalothin as a surrogate marker for cefpodoxime among urinary tract isolates. The MicroScan system (Siemens) was used to determine susceptibility for cephalothin and cefuroxime on consecutive cultures with a colony count of ≥50 000 organisms. Simul...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.063040-0

    authors: Bookstaver DA,Bland CM,Woodberry MW,Mansell KB

    更新日期:2014-02-01 00:00:00

  • Molecular diagnosis of rhino-orbito-cerebral mucormycosis from fresh tissue samples.

    abstract:PURPOSE:We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and the identification of fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). METHODOLOGY:Fifty cases of ROCM were included in the study. Conventional identification was performed using micros...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000560

    authors: Zaman K,Rudramurthy SM,Das A,Panda N,Honnavar P,Kaur H,Chakrabarti A

    更新日期:2017-08-01 00:00:00

  • Interleukin-8 release from human neutrophils after phagocytosis of Listeria monocytogenes and Yersinia enterocolitica.

    abstract::The release of interleukin-8 (IL-8) from human neutrophils was investigated after their internalisation of the invasive bacteria Listeria monocytogenes and Yersinia enterocolitica. Three pairs of bacterial strains were used to study the influence of listeriolysin O, invasiveness and Yersinia virulence plasmid (pYV)-en...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-1-55

    authors: Arnold R,König W

    更新日期:1998-01-01 00:00:00

  • Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36.

    abstract::This study investigated the molecular mechanisms of carbapenem resistance in clinical isolates of Klebsiella pneumoniae from Korea and the clinical outcomes of resistant K. pneumoniae infection. Sixteen K. pneumoniae isolates showing resistance to carbapenems collected from a tertiary-care hospital were examined for t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.037036-0

    authors: Shin SY,Bae IK,Kim J,Jeong SH,Yong D,Kim JM,Lee K

    更新日期:2012-02-01 00:00:00

  • In vitro activities of vancomycin and linezolid against biofilm-producing methicillin-resistant staphylococci species isolated from catheter-related bloodstream infections from an Egyptian tertiary hospital.

    abstract:PURPOSE:Catheter-related bloodstream infections (CRBSIs) are among the most common hospital-acquired infections. We aimed to survey methicillin resistance, biofilm production and susceptibility to vancomycin, linezolid and other antibiotics for staphylococci isolated from CRBSIs. METHODOLOGY:Fifty-eight isolates [20 S...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000490

    authors: Hashem AA,Abd El Fadeal NM,Shehata AS

    更新日期:2017-06-01 00:00:00

  • Potential of exocellular carbohydrate antigens of Staphylococcus epidermidis in the serodiagnosis of orthopaedic prosthetic infection.

    abstract::The potential of exocellular carbohydrate antigens of Staphylococcus epidermidis as markers of infection in bone was investigated by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Exocellular antigens were prepared by gel filtration chromatography of concentrated brain heart infusion culture supernates....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-44-5-355

    authors: Lambert PA,Van Maurik A,Parvatham S,Akhtar Z,Fraise AP,Krikler SJ

    更新日期:1996-05-01 00:00:00

  • In vitro antifungal activities of amphotericin B in combination with acteoside, a phenylethanoid glycoside from Colebrookea oppositifolia.

    abstract::This study was undertaken to investigate the synergistic interaction between amphotericin B (AmB) and acteoside, isolated from the aerial parts of the shrub Colebrookea oppositifolia (Lamiaceae). Acteoside alone exhibited no intrinsic antifungal activity but showed a potent synergism in combination with AmB against se...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.031906-0

    authors: Ali I,Sharma P,Suri KA,Satti NK,Dutt P,Afrin F,Khan IA

    更新日期:2011-09-01 00:00:00

  • Liver abscess caused by Brevundimonas vesicularis in an immunocompetent patient.

    abstract::Invasive infections caused by Brevundimonas vesicularis are very rare in humans. We experienced an unusual case of liver abscess due to B. vesicularis in an immunocompetent young male. The patient was successfully treated by liver abscess drainage and with antimicrobial therapy of ceftriaxone followed by ampicillin/su...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.045120-0

    authors: Yoo SH,Kim MJ,Roh KH,Kim SH,Park DW,Sohn JW,Yoon YK

    更新日期:2012-10-01 00:00:00

  • Mediterranean spotted fever with encephalitis.

    abstract::Rickettsia conorii infection is endemic in the Mediterranean basin, where it is known as Mediterranean spotted fever, also known as Boutonneuse fever and Marseilles fever. We report the case of a 66-year-old diabetic man who presented a severe form of the disease, complicated by acute renal failure, thrombocytopenia a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.004465-0

    authors: Aliaga L,Sánchez-Blázquez P,Rodríguez-Granger J,Sampedro A,Orozco M,Pastor J

    更新日期:2009-04-01 00:00:00

  • Opsonic requirements of Helicobacter pylori.

    abstract::The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-38-3-209

    authors: McKinlay AW,Young A,Russell RI,Gemmell CG

    更新日期:1993-03-01 00:00:00

  • Effect of chlorhexidine gluconate on the adherence of Candida species to denture acrylic.

    abstract::The effect of pretreatment of denture acrylic with chlorhexidine gluconate on the subsequent adherence of Candida albicans GDH 2346 was measured in vitro. Adherence was significantly reduced by pretreatment with chlorhexidine; maximal inhibition was achieved by incubation at room temperature for 30 min in 2% chlorhexi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-20-1-97

    authors: McCourtie J,MacFarlane TW,Samaranayake LP

    更新日期:1985-08-01 00:00:00

  • Mucosal and systemic antibody responses to the lipopolysaccharide of Escherichia coli O157 in health and disease.

    abstract::Mucosal immunity in the gastrointestinal (GI) tract is a primary defence against GI pathogens. We hypothesise that a mucosal response to lipopolysaccharide (LPS), especially to the common (core) determinants of GI pathogenic Escherichia coli strains, is protective. The aims of this study were to investigate the specif...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-4-345

    authors: Currie CG,McCALLUM K,Poxton IR

    更新日期:2001-04-01 00:00:00

  • Rapid antibiotic sensitivity testing and trimethoprim-mediated filamentation of clinical isolates of the Enterobacteriaceae assayed on a novel porous culture support.

    abstract::A porous inorganic material (Anopore) was employed as a microbial culture and microcolony imaging support. Rapid Anopore-based antibiotic sensitivity testing (AST) methods were developed to assess the growth of clinical isolates, with the primary focus on testing the response of the Enterobacteriaceae to trimethoprim,...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46585-0

    authors: Ingham CJ,van den Ende M,Wever PC,Schneeberger PM

    更新日期:2006-11-01 00:00:00

  • Molecular analysis of Shigella sonnei isolated from three well-documented outbreaks in school children.

    abstract::Fifty-eight isolates of Shigella sonnei from three outbreaks in school children and eight control isolates from epidemiologically unrelated sporadic clinical infections in Taiwan were compared by antibiotic susceptibility testing and molecular typing. Antibiotic susceptibility testing showed that all strains except on...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-4-355

    authors: Lee TM,Chang LL,Chang CY,Wang JC,Pan TM,Wang TK,Chang SF

    更新日期:2000-04-01 00:00:00

  • A novel real-time PCR to detect Chlamydia trachomatis in first-void urine or genital swabs.

    abstract::Screening for Chlamydia trachomatis infections can be performed on urine samples and genital swabs using molecular techniques. A novel approach was developed that combined an automated extraction procedure, an automated liquid-handling system and real-time PCR to detect C. trachomatis from urine or swabs. This novel r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46675-0

    authors: Jaton K,Bille J,Greub G

    更新日期:2006-12-01 00:00:00

  • Nucleolar accumulation of Semliki Forest virus nucleocapsid C protein: influence of metabolic status, cytoskeleton and receptors.

    abstract::The nucleolar accumulation of Semliki Forest Virus (SFV) C protein was examined as a function of intact microtubules, intact microfilaments and accessible intermediate filaments. The cytoskeletal components do not seem to play a role in directing C protein to the nucleolus but nucleolar accumulation is energy-dependen...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-6-389

    authors: Jakob R

    更新日期:1994-06-01 00:00:00

  • Assessment of Chlamydia trachomatis infection in asymptomatic male partners of infertile couples.

    abstract::Three specimens from 111 asymptomatic male partners of infertile couples attending the Department of Urology in Amiens, France, were examined by the PCR COBAS AMPLICOR test (Roche Molecular Diagnostics) for the presence of Chlamydia trachomatis. The specimens analysed were: first void urine (FVU), urine obtained after...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45641-0

    authors: Hamdad-Daoudi F,Petit J,Eb F

    更新日期:2004-10-01 00:00:00

  • ELISA for early diagnosis of histoplasmosis.

    abstract::An ELISA was developed and evaluated as a method for detecting antibodies against glycosylated and deglycosylated histoplasmin (HMIN). Sera from patients with histoplasmosis, paracoccidioidomycosis, sporotrichosis, coccidioidomycosis, aspergillosis, cryptococcosis and healthy donors were tested by ELISA against purifi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05469-0

    authors: Guimarães AJ,Pizzini CV,de Matos Guedes HL,Albuquerque PC,Peralta JM,Hamilton AJ,Zancopé-Oliveira RM

    更新日期:2004-06-01 00:00:00