Abstract:
:Alpha protoxin of Staphylococcus aureus "Wood 46" was activated by trypsin which had been coupled to carboxymethylcellulose, as indicated by the toxin's ability to hydrolyse tosyl-arginine methylester (TAME). A Lineweaver-Burk plot of the degradation of TAME by toxin and trypsin showed that toxin had a greater affinity for the substrate than had trypsin. N-terminal amino-acid analyses of activated toxin suggested that leucine or isoleucine is the N-terminus, in contrast to protoxin, the N-terminus of which is histidine.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Wiseman GM,Caird JD,Fackrell HBdoi
10.1099/00222615-8-1-29keywords:
subject
Has Abstractpub_date
1975-02-01 00:00:00pages
29-38issue
1eissn
0022-2615issn
1473-5644journal_volume
8pub_type
杂志文章abstract::To gain insight into the value of lipopolysaccharide (LPS) core determinants for cross-protective immunisation the serological relationships between six complete (LPS) core types from Enterobacteriaceae were investigated. Hyperimmune sera were raised in mice by repeated immunisation with heat-killed strains of Salmone...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-5-433
更新日期:1999-05-01 00:00:00
abstract::We describe the case of a graft versus host disease (GvHD) patient, in whom Hafnia alvei was cultured as a single organism, and at high bacterial counts from stool samples, from the onset of the disease until its resolution. This case is a further example of the contentious role of this species in causing human intest...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.2008/001164-0
更新日期:2008-09-01 00:00:00
abstract::Various factors influencing the susceptibility of C3H mice to lethal infection by the phycomycete Absidia ramosa were examined. Mice 19-21 days old were exposed to graded doses of A. ramosa spores by various routes. After intravenous inoculation with doses in excess of 10(3) viable units, a variable proportion of mice...
journal_title:Journal of medical microbiology
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doi:10.1099/00222615-8-4-551
更新日期:1975-11-01 00:00:00
abstract::Early detection of aetiological agents is pivotal for adequate therapy for bacterial infections. Although culture is still considered the mainstay for laboratory diagnosis, it often lacks sensitivity, especially in patients already treated with antibiotics. The present study investigated the potential clinical utility...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.034280-0
更新日期:2011-12-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-39-6-422
更新日期:1993-12-01 00:00:00
abstract::Spondylodiscitis caused by Aggregatibacter aphrophilus, formerly known as Haemophilus paraphrophilus, is an unusual condition and can be very difficult to diagnose. We report a case of cervical spondylodiscitis complicated by spinal epidural abscess in a 63-year-old woman, without underlying predisposing conditions. T...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47614-0
更新日期:2008-05-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47615-0
更新日期:2009-05-01 00:00:00
abstract::A provisional serotyping scheme for Bacillus cereus has been developed. Eighteen selected cultures of B. cereus isolated from foods and clinical specimens were used to prepare agglutinating sera against the flagellar antigen. There were no significant cross-reactions between the sera. The sera were used to type 137 is...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-8-4-543
更新日期:1975-11-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000256
更新日期:2016-06-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.040170-0
更新日期:2012-07-01 00:00:00
abstract::Production of a meningococcal vaccine capable of generating long-lasting immunity in all age groups is still a high priority worldwide. Iron-regulated outer-membrane proteins have attracted considerable attention in recent years and it has become increasingly evident that the meningococcal transferrin-binding proteins...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/00222615-44-4-237
更新日期:1996-04-01 00:00:00
abstract::Aim. The aim of this work was to characterize the response of Candida albicans to atorvastatin, and to assess its in vivo antifungal capability.Methodology. The effect of atorvastatin on the growth and viability of C. albicans was assessed. The ability of the statin to alter cell permeability was quantified by measuri...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001065
更新日期:2019-10-01 00:00:00
abstract::Fifty-five randomly selected Staphylococcus aureus strains were examined for fatty acid modifying enzyme (FAME) production. Of these, 20.4% did not elaborate the enzyme. Amongst the remaining strains, the lowest level produced in culture was 0.1 unit/10(9) cocci and the maximum was 2.01 U/10(9) cocci; the median level...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-4-235
更新日期:1992-10-01 00:00:00
abstract::The genus Eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. As a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remain...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-50-11-947
更新日期:2001-11-01 00:00:00
abstract:PURPOSE:This study aimed to evaluate the effect of two types of stress, cold and nutritional, on the viability and the in vitro virulence of the foodborne pathogenic bacteria Listeria monocytogenes. METHODOLOGY:Ten diverse isolates were kept in phosphate-buffered saline (PBS) at optimal (37 °C) or at refrigeration tem...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000648
更新日期:2018-01-01 00:00:00
abstract::Members of the Burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (CF) patients. The B. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. In this study, a new approach...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46457-0
更新日期:2006-06-01 00:00:00
abstract::A prospective study was conducted of the rapid FASTPlaque-Response test for determination of rifampicin resistance in Mycobacterium tuberculosis with and without the addition of an antimicrobial supplement containing nystatin, oxacillin and aztreonam (NOA) to control specimen-related contamination. A total of 631 smea...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46936-0
更新日期:2007-10-01 00:00:00
abstract::Formalin-killed cells of Pseudomonas aeruginosa strain M-24 elicited an antibody-independent protective effect against P. aeruginosa infection in mice. The effect was observed as early as 6 h after administration and 100% protection was obtained by 48 h. The protective effect could not be attributed to the production ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-28-2-101
更新日期:1989-02-01 00:00:00
abstract::Spirochaetes were demonstrated in material from patients with Lyme disease by short-time high-concentrate silver impregnation after treatment with amylase. Removal of mucoid material was essential to visualise Borrelia burgdorferi. Lyme spirochaetes were demonstrated in material from 23 patients with Lyme disease--ery...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-23-3-261
更新日期:1987-05-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-31-1-37
更新日期:1990-01-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000960
更新日期:2019-05-01 00:00:00
abstract::The main components of oral malodour have been identified as volatile sulfur compounds (VSCs), including hydrogen sulfide (H(2)S) and methyl mercaptan (CH(3)SH). The lactoperoxidase (LPO) system (consisting of LPO, glucose oxidase, glucose and thiocyanate) was previously shown to exhibit antimicrobial activities again...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000150
更新日期:2015-10-01 00:00:00
abstract::Antibody (AMI) and cell-mediated (CMI) immunity to rubella virus (RV) were evaluated in healthy adolescent males (n = 11) and females (n = 28) undergoing routine reimmunisation with RA27/3 strain RV as a component of measles-mumps-rubella (MMR) vaccine. Blood samples were collected just before and at 2, 4 and 10 weeks...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-12-1075
更新日期:1999-12-01 00:00:00
abstract::Acinetobacter junii is a rare human pathogen associated with bacteraemia in neonates and paediatric oncology patients. We present a case of A. junii causing bacteraemia in an adult transplant patient with leukaemia. The correct identification of Acinetobacter species can highlight the clinical significance of the diff...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.024596-0
更新日期:2011-03-01 00:00:00
abstract::In this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, Albicans ID(2) and Biggy agar, as well as onto Sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. These strains were identified as Candida glabrata in 20 (31....
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46625-0
更新日期:2007-06-01 00:00:00
abstract::The growth of 29 strains of Acinetobacter spp. on semi-solid media was studied; 19 showed surface swarming and 14 produced channels ("ditches") in the agar that do not seem to have been described previously. An attempt was made to define the cultural and physical conditions for the demonstration of these phenomena. Po...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-8-3-443
更新日期:1975-08-01 00:00:00
abstract::Brevundimonas diminuta is rarely associated with invasive infections. We report the case of a previously healthy young man with pleural effusion, in which B. diminuta was recovered but incorrectly identified as Kingella kingae when it was freshly isolated. Consequently, the misidentification resulted in initial treatm...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.045013-0
更新日期:2013-03-01 00:00:00
abstract::A partially-purified neuraminidase from the mucinase complex of Vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. When the neutralising potency of this serum against V. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-25-2-123
更新日期:1988-02-01 00:00:00
abstract::Thirty-seven multidrug-resistant and 13 pan-susceptible isolates of Mycobacterium tuberculosis were analysed for the diversity of genotypes associated with known drug-resistance mechanisms. The isolates were obtained from patients attending a university tuberculosis clinic in Monterrey, Mexico. A total of 25 IS6110-RF...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05343-0
更新日期:2004-02-01 00:00:00
abstract::The enzymic activity of 29 Haemophilus ducreyi strains on 28 substrates is described. The results are compared with those of seven other authors. There is agreement only about the presence of alkaline phosphatase and arginine aminopeptidase and the lack of glycosidases. Possible reasons for the contradictions in the e...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-18-2-181
更新日期:1984-10-01 00:00:00