Cutaneous leishmaniasis caused by Leishmania donovani in the tribal population of the Agasthyamala Biosphere Reserve forest, Western Ghats, Kerala, India.

Abstract:

:Cutaneous leishmaniasis (CL), a neglected tropical disease, is reported to be prevalent in tribal villages located in the Agasthyamala Biosphere Reserve forests of Western Ghats, Kerala state, India. We carried out an investigation to characterize the species of Leishmania parasites involved in these infections prevalent among one of the oldest human tribal populations in India. Skin aspirates collected from 13 clinically diagnosed cases were subjected to histopathological investigations, serological rapid tests using 'rk39' and molecular diagnostics. Clinical manifestations recorded among the patients were hypo-pigmented erythematous nodules/papules on limbs and other parts of the body. Histopathological investigations of these skin lesions among patients showed Leishman-Donovan bodies in macrophages. None of the patients were found to be positive for rk39 tests, which detect active visceral leishmaniasis. Using three different genetic markers [kinetoplast minicircle DNA, 3' UTR region of heat-shock protein 70 (Hsp70) and Hsp70 gene] we identified the parasite species involved in these infections to be Leishmania donovani. The 6-phosphogluconate (6-PGDH) gene sequences of the parasite isolates from Western Ghats indicated close genetic relatedness to L. donovani isolates reported from Sri Lanka, also causing CL. This could be cited as another instance of 'local endemism' of organisms in this single 'bio-geographic unit'.

journal_name

J Med Microbiol

authors

Kumar NP,Srinivasan R,Anish TS,Nandakumar G,Jambulingam P

doi

10.1099/jmm.0.076695-0

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

157-163

issue

Pt 2

eissn

0022-2615

issn

1473-5644

journal_volume

64

pub_type

杂志文章
  • Staphylococcal whole-cell polypeptide analysis: evaluation as a taxonomic and typing tool.

    abstract::Whole-cell-polypeptide profiles obtained by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) were used in conjunction with the API-Staph technique to identify different strains of Staphylococcus aureus, S. epidermidis, S. saprophyticus and S. capitis. Complete concordance of results from both techniques was achieved ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-23-1-41

    authors: Clink J,Pennington TH

    更新日期:1987-02-01 00:00:00

  • Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36.

    abstract::This study investigated the molecular mechanisms of carbapenem resistance in clinical isolates of Klebsiella pneumoniae from Korea and the clinical outcomes of resistant K. pneumoniae infection. Sixteen K. pneumoniae isolates showing resistance to carbapenems collected from a tertiary-care hospital were examined for t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.037036-0

    authors: Shin SY,Bae IK,Kim J,Jeong SH,Yong D,Kim JM,Lee K

    更新日期:2012-02-01 00:00:00

  • Effect of carbon dioxide on the growth and form of Candida albicans.

    abstract::The effect of CO2 on the growth of 31 strains of Candida albicans was studied in serum and in a defined medium containing urea, ammonium chloride, asparagine, glutamine or acetamide as the nitrogen source. CO2 10% enhanced the mycelial growth of all strains when the medium contained an appropriate constituent to media...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-22-3-203

    authors: Sims W

    更新日期:1986-11-01 00:00:00

  • Production and properties of haemolysins from clinical isolates of the Proteeae.

    abstract::A collection of 198 clinical isolates of strains belonging to the tribe Proteeae was examined for haemolytic activity on blood agar and in Brain Heart Infusion Broth. The strains were of diverse bacteriocin and O-antigenic types and from a wide variety of sources. They included representatives of all species of Morgan...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-25-1-17

    authors: Senior BW,Hughes C

    更新日期:1988-01-01 00:00:00

  • In-vitro proteinase production by oral Candida albicans isolates from individuals with and without HIV infection and its attenuation by antimycotic agents.

    abstract::In-vitro proteinase production by oral Candida albicans isolates from patients with and without HIV infection (18 isolates from each group) was assessed by image analysis of a plate assay, with bovine serum albumin (BSA) as a substrate. The effect of sub-minimal inhibitory concentrations (sub-MICs) of nystatin, amphot...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-44-4-311

    authors: Wu T,Samaranayake LP,Cao BY,Wang J

    更新日期:1996-04-01 00:00:00

  • Lesions produced by Clostridium butyricum strain CB 1002 in ligated intestinal loops in guinea pigs.

    abstract::Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-19-3-351

    authors: Popoff MR,Ravisse P

    更新日期:1985-06-01 00:00:00

  • Comparison of conventional culture with SeptiFast real-time PCR for microbial pathogen detection in clinical specimens other than blood.

    abstract::Early detection of aetiological agents is pivotal for adequate therapy for bacterial infections. Although culture is still considered the mainstay for laboratory diagnosis, it often lacks sensitivity, especially in patients already treated with antibiotics. The present study investigated the potential clinical utility...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034280-0

    authors: Mencacci A,Leli C,Cardaccia A,Montagna P,Moretti A,Bietolini C,Meucci M,Perito S,Cenci E,Bistoni F

    更新日期:2011-12-01 00:00:00

  • Exceptionally high titres in atypical presentation of occult epididymo-orchitis due to brucellosis.

    abstract::A male patient of 32 years was referred for surgical drainage and orchidectomy of the right testis following a cycling injury. A Venereal Disease Research Laboratory (VDRL) test was requested by the surgery department to rule out secondary syphilis. Although serum samples gave a negative result in the VDRL test, quali...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034892-0

    authors: Purwar S,Metgud SC,Gokale SK

    更新日期:2012-03-01 00:00:00

  • Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995-1998.

    abstract::A total of 3429 isolations of verocytotoxin-producing Escherichia coli O157 (VTEC O157) was confirmed from human sources in England and Wales during the period 1995-1998. The largest annual total was 1087 in 1997. Most infections occurred in the third quarter of each year. The overall rate of infection ranged from 1.2...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-2-135

    authors: Willshaw GA,Cheasty T,Smith HR,O'Brien SJ,Adak GK

    更新日期:2001-02-01 00:00:00

  • Changes in genetic types and population dynamics of Moraxella catarrhalis in hospitalized children are not associated with an exacerbation of existing disease.

    abstract::Pulsed-field gel electrophoresis typing was performed on a retrospective set of 129 Moraxella catarrhalis isolates obtained over a 20 month period from 70 children admitted to, or presenting at, the Erasmus University Medical Center, Rotterdam, The Netherlands. The mean age of the children (at the end of the study) wa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05251-0

    authors: Hays JP,Eadie K,Verduin CM,Hazelzet J,Verbrugh H,van Belkum A

    更新日期:2003-09-01 00:00:00

  • Isolation and characterisation of an anticryptococcal protein in human cerebrospinal fluid.

    abstract::An earlier study reported that human cerebrospinal fluid (CSF) has fungistatic activity for Cryptococcus neoformans. The present study reports that molecular sieve fractionation of concentrated CSF yielded three protein peaks, one of which (p2) had anticryptococcal activity. On a DEAE-Sephacel anion-exchange column th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-1-83

    authors: Ahluwalia M,Brummer E,Sridhar S,Singh R,Stevens DA

    更新日期:2001-01-01 00:00:00

  • Molecular epidemiology of Bordetella pertussis in Greece, 2010-2015.

    abstract:PURPOSE:To determine the predominant strains of Bordetella pertussis in Greece during 2010-2015. METHODOLOGY:Infants and children (n=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS481/IS1001 r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000688

    authors: Petridou E,Jensen CB,Arvanitidis A,Giannaki-Psinaki M,Michos A,Krogfelt KA,Petersen RF

    更新日期:2018-03-01 00:00:00

  • Characteristics of hospital- and community-acquired meticillin-resistant Staphylococcus aureus in Tehran, Iran.

    abstract::Staphylococcus aureus is a leading cause of hospital-acquired (HA) and community-acquired (CA) infections worldwide. Recently, S. aureus strains resistant to meticillin (MRSA) have become established within both communities. We isolated 314 isolates of MRSA from hospitalized patients in a referral hospital (HA isolate...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.070722-0

    authors: Rahimi F,Katouli M,Pourshafie MR

    更新日期:2014-06-01 00:00:00

  • Two Japanese Corynebacterium ulcerans isolates from the same hospital: ribotype, toxigenicity and serum antitoxin titre.

    abstract::Two toxigenic Corynebacterium ulcerans isolates recovered from pharyngeal swabs of two patients from the same hospital in Japan during 2001-2002 were characterized by PFGE and ribotyping. Toxin production in different culture media was examined and serological analysis of patient sera was performed. The two isolates c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.022491-0

    authors: Komiya T,Seto Y,De Zoysa A,Iwaki M,Hatanaka A,Tsunoda A,Arakawa Y,Kozaki S,Takahashi M

    更新日期:2010-12-01 00:00:00

  • A simple and safe volumetric alternative to the method of Miles, Misra and Irwin for counting viable bacteria.

    abstract::A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-4-541

    authors: Slack MP,Wheldon DB

    更新日期:1978-11-01 00:00:00

  • Improved rapid molecular diagnosis of multidrug-resistant tuberculosis using a new reverse hybridization assay, REBA MTB-MDR.

    abstract::Rapid diagnosis of multidrug-resistant tuberculosis (MDR-TB) is essential for the prompt initiation of effective second-line therapy to improve treatment outcome and limit transmission of this obstinate disease. A variety of molecular methods that enable the rapid detection of mutations implicated in MDR-TB have been ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.032292-0

    authors: Bang H,Park S,Hwang J,Jin H,Cho E,Kim DY,Song T,Shamputa IC,Via LE,Barry CE,Cho SN,Lee H

    更新日期:2011-10-01 00:00:00

  • Opsonic requirements of Helicobacter pylori.

    abstract::The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-38-3-209

    authors: McKinlay AW,Young A,Russell RI,Gemmell CG

    更新日期:1993-03-01 00:00:00

  • The effect of human serum DNAases on the ability to detect antibiotic-killed Escherichia coli in blood by PCR.

    abstract::PCR has proved superior to conventional blood culture for diagnosing bacteraemia in the presence of antibiotics. Nevertheless, even PCR might yield false-negative results if the template DNA were to be cleaved by serum DNAases after antibiotics had induced bacterial death. To evaluate the cleavage of bacterial templat...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-3-243

    authors: Heininger A,Ulrich M,Priebe G,Unertl K,Müller-Schauenburg A,Botzenhart K,Döring G

    更新日期:2001-03-01 00:00:00

  • Inhibition by amoeba-specific antiserum and by cytochalasin B of the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures.

    abstract::Inhibitors of trophozoite motility and phagocytosis were used to investigate the mechanism of Naegleria fowleri cytopathogenicity in mouse-embryo (ME)-cell cultures. Amoebae that were immobilised and agglutinated by specific antiserum exhibited no cytopathic activity, although they remained alive and were in constant ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-3-355

    authors: Brown T

    更新日期:1979-08-01 00:00:00

  • Nucleolar accumulation of Semliki Forest virus nucleocapsid C protein: influence of metabolic status, cytoskeleton and receptors.

    abstract::The nucleolar accumulation of Semliki Forest Virus (SFV) C protein was examined as a function of intact microtubules, intact microfilaments and accessible intermediate filaments. The cytoskeletal components do not seem to play a role in directing C protein to the nucleolus but nucleolar accumulation is energy-dependen...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-6-389

    authors: Jakob R

    更新日期:1994-06-01 00:00:00

  • Human epithelial cell death caused by Actinobacillus actinomycetemcomitans infection.

    abstract::The gingival sulcus is the shallow crevice around the tooth, and its epithelium is a gateway for initial bacterial infection in periodontal disease. Recent studies have shown that Actinobacillus actinomycetemcomitans invades an epithelial cell line, KB cells, in vitro. The aim of the present study was to clarify the c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-8-739

    authors: Kato S,Nakashima K,Inoue M,Tomioka J,Nonaka K,Nishihara T,Kowashi Y

    更新日期:2000-08-01 00:00:00

  • Host and pathogen factors in Klebsiella pneumoniae upper urinary tract infections in renal transplant patients.

    abstract:PURPOSE:To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients. METHODOLOGY:Clinical and demographic data were registered prospectively. Phylogenetic background of K. pneumoniae isolates was analysed by PCR melting pro...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000942

    authors: Gołębiewska JE,Krawczyk B,Wysocka M,Ewiak A,Komarnicka J,Bronk M,Rutkowski B,Dębska-Ślizień A

    更新日期:2019-03-01 00:00:00

  • Studies on early association of Salmonella typhimurium with intestinal mucosa in vivo and in vitro: relationship to virulence.

    abstract::The abilities of six strains of Salmonella typhimurium to associate with rabbit ileal mucosa have been measured in vitro. Two were "virulent" strains (TML and W118 which are invasive and inducers of fluid secretion in rabbit ileal loops); four were "avirulent" (LT7, M206 and SL1027 which are invasive but induce neglig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-29-4-283

    authors: Worton KJ,Candy DC,Wallis TS,Clarke GJ,Osborne MP,Haddon SJ,Stephen J

    更新日期:1989-08-01 00:00:00

  • Inhibition of streptolysin O by allicin - an active component of garlic.

    abstract::Streptolysin O (SLO) is a potent cytolytic toxin produced by almost all strains of group A streptococci and is considered an important virulence factor for this organism. In this study we investigated the effect of allicin and aqueous garlic extracts on the haemolytic activity of SLO. All tested materials potentially ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019539-0

    authors: Arzanlou M,Bohlooli S

    更新日期:2010-09-01 00:00:00

  • Adhesion of fimbriate Escherichia coli to bovine mammary-gland epithelial cells in vitro.

    abstract::The adhesion of bovine and a human isolate of Escherichia coli to epithelial cells from the teat and lactiferous sinuses of the udder was examined. Adhesion was detected with bacterial suspensions that produced mannose-sensitive agglutination of guinea-pig red cells. Adhesion to epithelial cells could be inhibited by ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-2-117

    authors: Harper M,Turvey A,Bramley AJ

    更新日期:1978-05-01 00:00:00

  • Prevalence and molecular epidemiology of plasmid-mediated fosfomycin resistance genes among blood and urinary Escherichia coli isolates.

    abstract::A total of 1878 non-duplicate clinical Escherichia coli isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996-2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin (fos) resistanc...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.062653-0

    authors: Ho PL,Chan J,Lo WU,Lai EL,Cheung YY,Lau TCK,Chow KH

    更新日期:2013-11-01 00:00:00

  • Bone resorbing activity of surface-associated material from Actinobacillus actinomycetemcomitans and Eikenella corrodens.

    abstract::The results of this study demonstrate that saline extracted surface-associated material (SAM) of Actinobacillus actinomycetemcomitans and Eikenella corrodens stimulates bone resorption at picomolar concentrations. Various inhibitors of known osteolytic mediators--indomethacin, interleukin-1 receptor antagonist (IL-1ra...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-3-197

    authors: Meghji S,Wilson M,Barber P,Henderson B

    更新日期:1994-09-01 00:00:00

  • A severe outbreak of listeriosis in central Italy with a rare pulsotype associated with processed pork products.

    abstract:PURPOSE:From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidiscipl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000785

    authors: Duranti A,Sabbatucci M,Blasi G,Acciari VA,Ancora M,Bella A,Busani L,Centorame P,Cammà C,Conti F,De Medici D,Di Domenico M,Di Marzio V,Filippini G,Fiore A,Fisichella S,Gattuso A,Gianfranceschi M,Graziani C,Guidi F,

    更新日期:2018-09-01 00:00:00

  • The establishment and persistence of Ureaplasma urealyticum in oestradiol-treated female mice.

    abstract::Administration of oestradiol to three strains of female mice induced the oestrous phase of the reproductive cycle in which there are few or no polymorphonuclear leucocytes (PMNL) in vaginal smears. This treatment rendered the mice susceptible to genital tract colonisation by serotype 8 of Ureaplasma urealyticum, inocu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-29-2-111

    authors: Furr PM,Taylor-Robinson D

    更新日期:1989-06-01 00:00:00

  • Immunological detection of Bacteroides fragilis in clinical samples.

    abstract::A monospecific polyclonal antiserum, prepared against Bacteroides fragilis common polysaccharide antigen purified by polyacrylamide gel immunoblot detected B. fragilis, B. thetaiotaomicron, B. ovatus and Prevotella melaninogenica in pus samples from various anatomical sites by immunofluorescence microscopy of the pus....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-2-99

    authors: Patrick S,Stewart LD,Damani N,Wilson KG,Lutton DA,Larkin MJ,Poxton I,Brown R

    更新日期:1995-08-01 00:00:00