Improvement and optimization of the classical gastric biopsy culture technique for Helicobacter pylori diagnosis using trypsin.

abstract：:The aim of the present study was to investigate the colonization status of Clostridium difficile in healthy individuals. In total, 139 healthy adults from two study groups were examined at intervals of 3 months. Among the 18 positive subjects, the number of subjects from whom C. difficile was isolated once, twice, thr...

journal_title：Journal of medical microbiology

authors： Ozaki E,Kato H,Kita H,Karasawa T,Maegawa T,Koino Y,Matsumoto K,Takada T,Nomoto K,Tanaka R,Nakamura S

更新日期：2004-02-01 00:00:00

abstract：:Some strains of Helicobacter pylori are known to produce an extracellular cytotoxin that causes vacuolation in cultured mammalian cells. Screening for such strains makes use of HeLa cells which may not be sensitive enough to detect minimal changes. The aim of this study was to develop a more sensitive cell line. Vacuo...

journal_title：Journal of medical microbiology

authors： Wang XM,Kojima T,Satoh K,Taniguchi Y,Tokumaru K,Saifuku K,Seki M,Kihira K,Ido K,Uchida JY,Ohmori C,Takaoka T,Kimura K

更新日期：1997-08-01 00:00:00

abstract：:In Mexico, there is a lack of up-to-date published data that show viruses to be the main cause of acute respiratory infection (ARI). The objective of this study was to estimate the comparative viral frequency between children under the age of 5 years with and without ARI (n = 179 in each group) in a suburban community...

journal_title：Journal of medical microbiology

authors： Manjarrez ME,Rosete DP,Rincón M,Villalba J,Cravioto A,Cabrera R

更新日期：2003-07-01 00:00:00

abstract：:PCR has proved superior to conventional blood culture for diagnosing bacteraemia in the presence of antibiotics. Nevertheless, even PCR might yield false-negative results if the template DNA were to be cleaved by serum DNAases after antibiotics had induced bacterial death. To evaluate the cleavage of bacterial templat...

journal_title：Journal of medical microbiology

authors： Heininger A,Ulrich M,Priebe G,Unertl K,Müller-Schauenburg A,Botzenhart K,Döring G

更新日期：2001-03-01 00:00:00

abstract：:Bovine lactoferricin, a pepsin-generated antimicrobial peptide from bovine lactoferrin active against a wide range of bacteria, was tested for its ability to influence the adhesion and invasion of Yersinia enterocolitica and Yersinia pseudotuberculosis in HEp-2 cells. The addition of non-cytotoxic and non-bactericidal...

journal_title：Journal of medical microbiology

authors： Di Biase AM,Tinari A,Pietrantoni A,Antonini G,Valenti P,Conte MP,Superti F

更新日期：2004-05-01 00:00:00

abstract：:The frequency distribution of plasmids in a representative collection of Escherichia coli and other enterobacteria was compared with the frequencies predicted by the Poisson distribution. The distribution of E. coli plasmids did not differ significantly (p greater than 0.2) whereas the difference between observed and ...

journal_title：Journal of medical microbiology

authors： Platt DJ

更新日期：1987-05-01 00:00:00

abstract：:Nasal polyp cells in primary culture from cystic fibrosis (CF) and non-CF patients were compared for the ability to bind Pseudomonas aeruginosa cells and for the presence of sulphated glycoconjugates at the epithelial cell surface. Quantitation of bacterial adhesion, by scanning electronmicroscopy, showed no significa...

journal_title：Journal of medical microbiology

authors： Plotkowski MC,Chevillard M,Pierrot D,Altemayer D,Puchelle E

更新日期：1992-02-01 00:00:00

abstract：:Borderline oxacillin-resistant Staphylococcus aureus (BORSA) represents a quite poorly understood and inadequately defined phenotype of methicillin resistance. BORSA strains show low, borderline resistance to penicillinase-resistant penicillins (PRPs), with oxacillin MICs typically equal to 1-8 µg ml-1, and in contras...

journal_title：Journal of medical microbiology

authors： Hryniewicz MM,Garbacz K

更新日期：2017-10-01 00:00:00

abstract：:A retrospective case-control study of 118 (male : female, 68 : 50) Chinese type 2 diabetic patients with foot ulcers (Wagner's grade 3-5) was conducted to determine the prevalence and risk factors for meticillin-resistant Staphylococcus aureus (MRSA) infection in relation to the original community or hospital paramete...

journal_title：Journal of medical microbiology

authors： Wang SH,Sun ZL,Guo YJ,Yang BQ,Yuan Y,Wei Q,Ye KP

更新日期：2010-10-01 00:00:00

abstract：:A case of Salmonella typhimurium pericarditis is reported. The diagnosis was based on blood and pericardial effusion cultures. ...

journal_title：Journal of medical microbiology

authors： Can F,Demirbilek M,Erdem B,Ciftci U,Tunaoglu M,Laleli Y

更新日期：2004-10-01 00:00:00

abstract：:In recent years, several plasmids harbouring genes encoding phosphoethanolamine transferases conferring colistin resistance have been described in multiple Enterobacteriaceae species. Avian Pathogenic E. coli (APEC) causes colibacillosis and is responsible for a considerable proportion of the disease burden in commerc...

journal_title：Journal of medical microbiology

authors： Hornsey M,Betts JW,Mehat JW,Wareham DW,van Vliet AHM,Woodward MJ,La Ragione RM

更新日期：2019-01-01 00:00:00

abstract：:The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies....

journal_title：Journal of medical microbiology

authors： Barlow REL,Cooke ID,Odukoya O,Heatley MK,Jenkins J,Narayansingh G,Ramsewak SS,Eley A

更新日期：2001-10-01 00:00:00

abstract：:The therapeutic alternatives available for use against ciprofloxacin-resistant enteric fever isolates in an endemic area are limited. The antibiotics currently available are the quinolones, third-generation cephalosporins and conventional first-line drugs. In this study, the MICs of various newer drugs were determined...

journal_title：Journal of medical microbiology

authors： Capoor MR,Rawat D,Nair D,Hasan AS,Deb M,Aggarwal P,Pillai P

更新日期：2007-11-01 00:00:00

abstract：:Blood cultures are sometimes obtained from intravascular catheters for convenience. However, there is controversy regarding this practice. The authors compared the diagnostic test characteristics of blood cultures obtained from intravascular catheters and peripheral veins. Relevant studies for inclusion in this review...

journal_title：Journal of medical microbiology

authors： Falagas ME,Kazantzi MS,Bliziotis IA

更新日期：2008-01-01 00:00:00

abstract：:A study of 1314 patients with malignancies was made to determine the prevalence of Serratia marcescens in surveillance, diagnostic, and environmental cultures. Sera obtained from a commercial source were used to determine serotypic distributions of the S. marcescens strains isolated during a 51-month period. S. marces...

journal_title：Journal of medical microbiology

authors： Young VM,Moody MR,Morris MJ

更新日期：1980-05-01 00:00:00

abstract：:Chronic meningococcaemia is an unusual clinical presentation of Neisseria meningitidis infection. We describe the case of a patient, who presented with total IgA deficiency and partial IgM deficiency with a low switched memory B cells count, suggestive of a borderline form of common variable immunodeficiency (CVID). T...

journal_title：Journal of medical microbiology

authors： Theulin A,Rondeau-Lutz M,Kuhnert C,Boileau J,Weber JC

更新日期：2010-11-01 00:00:00

abstract：:Spondylodiscitis caused by Aggregatibacter aphrophilus, formerly known as Haemophilus paraphrophilus, is an unusual condition and can be very difficult to diagnose. We report a case of cervical spondylodiscitis complicated by spinal epidural abscess in a 63-year-old woman, without underlying predisposing conditions. T...

journal_title：Journal of medical microbiology

authors： Pasqualini L,Mencacci A,Scarponi AM,Leli C,Fabbriciani G,Callarelli L,Schillaci G,Bistoni F,Mannarino E

更新日期：2008-05-01 00:00:00

abstract：:Yersinia pseudotuberculosis was examined for its haemagglutinating activity and adherence to cultured epithelial cells (HEp-2) in relation to possession of a virulence (VW) plasmid and to growth conditions. VW-lacking (VW-) bacteria were isolated from ten VW+ strains of each serovar which, after they were grown on CFA...

journal_title：Journal of medical microbiology

authors： Kawaoka Y,Mitani T,Hoshina K,Otsuki K,Tsubokura M

更新日期：1988-03-01 00:00:00

abstract：:A polymerase chain reaction (PCR) assay with oligonucleotide primers homologous to a portion of the urease C gene of Helicobacter pylori was evaluated for specificity with pure DNA and biopsy material. The assay was used to test for the presence of the organism in dental plaque. The species specificity of detection wa...

journal_title：Journal of medical microbiology

authors： Bickley J,Owen RJ,Fraser AG,Pounder RE

更新日期：1993-11-01 00:00:00

abstract：:Urinary isolates of Escherichia coli that were resistant to trimethoprim were collected in Glasgow Royal Infirmary during 1979 and 1980. Eighty-eight were resistant to trimethoprim 1024 micrograms/ml and 80 (92%) were also resistant to sulphamethoxazole 1024 micrograms/ml; 73% were multiresistant. Plasmids were detect...

journal_title：Journal of medical microbiology

authors： Kraft CA,Platt DJ,Timbury MC

更新日期：1983-11-01 00:00:00

abstract：:Clostridium perfringens type A produces an enterotoxin that induces diarrhoea experimentally in man and animals. The enterotoxin causes increased membrane permeability in susceptible cells which is thought to be due to pore formation in the host cell membrane. The effect of purified C. perfringens enterotoxin on intac...

journal_title：Journal of medical microbiology

authors： Hardy SP,Denmead M,Parekh N,Granum PE

更新日期：1999-03-01 00:00:00

abstract：:The rat was evaluated as an experimental model for disseminated candidosis by quantitating blood clearance and initial organ localisation of 3H-leucine-labelled Candida albicans after intravenous injection into the tail or portal vein. Viable or formalin-killed blastoconidia or viable blastoconidia with germ tubes wer...

journal_title：Journal of medical microbiology

authors： Katz S,Grosfeld JL,Folkening WJ,Rosenthal RS,Merkel GJ

更新日期：1992-10-01 00:00:00

abstract：:The microbiota of the human oropharynx plays an important role in health through involvement in the aetiology of infection and the carriage of adventitious pathogens. Despite this, there are few models available for the preclinical assessment of novel antimicrobials directed to the human throat. We have profiled bacte...

journal_title：Journal of medical microbiology

authors： Humphreys GJ,McBain AJ

更新日期：2013-06-01 00:00:00

abstract：:A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (two), Epidermophyton (one), Microsporum (three) and Trichophyton (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS...

journal_title：Journal of medical microbiology

authors： De Baere T,Summerbell R,Theelen B,Boekhout T,Vaneechoutte M

更新日期：2010-01-01 00:00:00

abstract：:Ruminants are a major reservoir of enterohaemorrhagic Escherichia coli (EHEC), which cause acute gastroenteritis in humans with potentially life-threatening sequelae. The mechanisms underlying EHEC persistence in ruminant hosts are poorly understood. EHEC produce several cytotoxins that inhibit the proliferation of bo...

journal_title：Journal of medical microbiology

authors： Menge C,Stamm I,van Diemen PM,Sopp P,Baljer G,Wallis TS,Stevens MP

更新日期：2004-06-01 00:00:00

abstract：:The aim of this study was to investigate the diversity and composition of the intestinal microbiota of elderly subjects using a combination of culture-dependent techniques and 16S rRNA gene amplicon sequencing. The study was performed as part of the ELDERMET project, in which 368 faecal samples were assessed for viabl...

journal_title：Journal of medical microbiology

authors： Lakshminarayanan B,Harris HMB,Coakley M,O'Sullivan Ó,Stanton C,Pruteanu M,Shanahan F,O'Toole PW,Ross RP,On Behalf Of The Eldermet Consortium

更新日期：2013-03-01 00:00:00

abstract：:The unpredictable characteristics of meningococcal disease (MD) make outbreaks complicated to monitor and consequently lead to high levels of public anxiety. Traditional molecular techniques have been utilized in order to understand better the epidemiology of MD, but some have disadvantages such as being highly specia...

journal_title：Journal of medical microbiology

authors： Diggle MA,Bell CM,Clarke SC

更新日期：2003-06-01 00:00:00

abstract：:Introduction.Candida auris is a pathogenic yeast that mainly affects immunosuppressed patients and those with implanted medical devices. This pathogen also displays elevated resistance to common antifungals and high survival and spreading capacities. Since no antifungal breakpoints have yet been defined for this patho...

journal_title：Journal of medical microbiology

authors： Romera D,Aguilera-Correa JJ,Gadea I,Viñuela-Sandoval L,García-Rodríguez J,Esteban J

更新日期：2019-09-01 00:00:00

abstract：PURPOSE:Strains of type emm89 Streptococcus pyogenes have recently increased in frequency as a cause of human infections in several countries in Europe and North America. This increase has been molecular epidemiologically linked with the emergence of a new genetically distinct clone, designated clade 3. We sought to ex...

journal_title：Journal of medical microbiology

authors： Beres SB,Olsen RJ,Ojeda Saavedra M,Ure R,Reynolds A,Lindsay DSJ,Smith AJ,Musser JM

更新日期：2017-12-01 00:00:00

abstract：:To analyse the characteristics of infections caused by Streptococcus dysgalactiae subsp. equisimilis, clinical isolates (n=145) were collected at 11 medical institutions between September 2003 and October 2005. These isolates belonged to Lancefield group A (n=5), group C (n=18) or group G (n=122). Among all isolates, ...

journal_title：Journal of medical microbiology

authors： Sunaoshi K,Murayama SY,Adachi K,Yagoshi M,Okuzumi K,Chiba N,Morozumi M,Ubukata K

更新日期：2010-01-01 00:00:00