Abstract:
:Most hospital bacteriologists have divided staphylococci into two groups: Staphylococcus aureus and the coagulase-negative staphylococci of which the novobiocin-resistant varieties are termed S. saprophyticus. The identification of S. aureus has been easy but that of the other staphylococci has provided some difficulties and most currently available methods are expensive or time consuming. Multipoint inoculation of a set of test media provides a convenient way of identifying large numbers of staphylococcal isolates. In tests with 118 isolates, mainly clinical but including some environmental isolates and some from the National Collection of Type Cultures, there was 90.7% agreement between identifications by the API-Staph system and by the multipoint system. The remaining 9.3% of strains was identified by the multipoint system but could not be identified by use of the data supplied in the API-Staph kit.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Reuther JWdoi
10.1099/00222615-22-2-179subject
Has Abstractpub_date
1986-09-01 00:00:00pages
179-82issue
2eissn
0022-2615issn
1473-5644journal_volume
22pub_type
杂志文章abstract::Of 200 isolates of Vibrio mimicus screened, one from water (N-57) agglutinated with V. cholerae O139 polyclonal antiserum (absorbed with a rough strain of V. cholerae only) and not with O139 polyclonal diagnostic antiserum (absorbed with the rough strain and V. cholerae O22 and O155). The antigenic relationship betwee...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-9-873
更新日期:1999-09-01 00:00:00
abstract::Clinical isolates of Neisseria gonorrhoeae harbouring resistance (R) plasmids of mol. wts 4.4 x 10(6) (Asian) or 3.2 x 10(6) (African) were grown in prolonged glucose-limited continuous culture to determine the segregation efficiency of each type of plasmid and their expression of penicillinase activity in the absence...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-24-4-351
更新日期:1987-12-01 00:00:00
abstract::Phenotypic loss of protein A production was tested in six methicillin-resistant (McR) Staphylococcus aureus (MRSA) isolates and their isogenic methicillin-sensitive (McS) variants by a radiolabelled IgG-binding assay with washed cells and by Western blotting of supernates prepared from lysed washed cells. Genomic DNA ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-39-6-422
更新日期:1993-12-01 00:00:00
abstract::Antibiotic resistance in Staphylococcus aureus is a major public health concern, and methicillin-resistant S. aureus has emerged as an important pathogen. We characterized S. aureus isolates from monomicrobial and polymicrobial wound infections from 200 diabetic individuals with foot ulcers to understand their underly...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000370
更新日期:2016-12-01 00:00:00
abstract::Burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. However, recent surveys in Thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. To determine whether strai...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-7-565
更新日期:1997-07-01 00:00:00
abstract:PURPOSE:This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000548
更新日期:2017-07-01 00:00:00
abstract::The association of Aeromonas spp. with travellers' diarrhoea was studied among 978 Finnish tourists travelling to Morocco in winter (n = 398) and autumn (n = 580) in 1989. Fifty-five isolates from diarrhoeal patients with (n = 16) or without (n = 39) a recent travelling history in a developing country were also includ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-42-1-26
更新日期:1995-01-01 00:00:00
abstract::In a prospective study between February 2003 and June 2004, stool specimens of children less than 2 years of age with diarrhoea (n=218) and without diarrhoea (n=86), living in Vitória, Espírito Santo, Brazil, were examined for the presence of diarrhoeagenic Escherichia coli. E. coli isolates were tested by colony blot...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47660-0
更新日期:2008-03-01 00:00:00
abstract::Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-12-987
更新日期:1997-12-01 00:00:00
abstract::A collection of Enterococcus faecalis strains from clinical isolates, healthy individuals and the environment was screened for the presence of virulence factor genes, such as those for collagen-binding protein (ace), endocarditis antigen (efaA), haemolysin activator (cylA), gelatinase (gelE), aggregation substances (a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05353-0
更新日期:2004-01-01 00:00:00
abstract::A novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome (FTLS) was discovered in Henan Province, China. Here, we report the development of an assay for this novel bunyavirus based on real-time reverse transcription PCR (RT-PCR). The assay exhibited high sensitivity and specificity without cr...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.049577-0
更新日期:2013-07-01 00:00:00
abstract::Erythrocyte-sensitising antigens (AE) were prepared from Vibrio cholerae serotypes, from EL-Tor vibrio, Escherichia coli and Salmonella enteritidis by digesting the organisms with NaOH followed by precipitation with alcohol. When AE was used in indirect haemagglutination (IHA) tests, the results in a number of cases w...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-8-1-11
更新日期:1975-02-01 00:00:00
abstract::The prevalence of community-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) strains has become a serious problem worldwide. The aim of this study was to investigate the annual transitions of MRSA strains with the CA-MRSA feature, which were identified as SCCmec type IV or V, in a hospital setting in Japa...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000080
更新日期:2015-07-01 00:00:00
abstract::Rosacea is a chronic inflammatory condition that affects the skin of the face and the eyes. The aetiology of rosacea is not clearly established but increasing evidence suggests a potential role for bacteria in the induction of the condition. A role for Bacillus oleronius, originally isolated from within a Demodex foll...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.065136-0
更新日期:2014-02-01 00:00:00
abstract::A collection of 201 isolates of Staphylococcus aureus was examined: 152 methicillin-sensitive S. aureus (MSSA) comprised 48 blood culture isolates (BC) and 58 isolates from routine diagnostic specimens (RD) from Glasgow Royal Infirmary (GRI), and 46 strains from nasal swabs of patients attending a general practitioner...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-3-164
更新日期:1992-03-01 00:00:00
abstract::Bacterial infection in patients who have undergone liver transplantation is a major complication of the procedure. Leuconostoc spp. are important pathogenic bacteria in individuals with poor immune function, especially transplant patients. In this report, we describe the case of a 45-year-old Asian male liver transpla...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.045161-0
更新日期:2012-11-01 00:00:00
abstract::A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-43-4-282
更新日期:1995-10-01 00:00:00
abstract::The possession of any of eight different plasmids by Staphylococcus aureus strain 649--either singly or simultaneously (in no. 649MR)--caused changes in growth kinetics. Six of the plasmids caused an increase in exponential doubling time (by 8-25%), and most also altered the duration of the lag period. Strain 649MR wa...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-8-1-137
更新日期:1975-02-01 00:00:00
abstract::Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-33-4-217
更新日期:1990-12-01 00:00:00
abstract::Rhodococcus equi is an opportunistic human pathogen associated with immunosuppressed people. While the interaction of R. equi with macrophages has been comprehensively studied, little is known about its interactions with non-phagocytic cells. Here, we characterized the entry process of this bacterium into human lung e...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.056234-0
更新日期:2013-08-01 00:00:00
abstract::The susceptibility of chicks to enteritis caused by Campylobacter jejuni was studied. Three-day-old chicks did not develop enteritis after oral infection but chicks infected within 12 h of hatching developed gastroenteritis. The incubation period correlated with the inoculum size. Initially, infected chicks developed ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-18-2-233
更新日期:1984-10-01 00:00:00
abstract::Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, th...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-45-5-349
更新日期:1996-11-01 00:00:00
abstract::Spirochaetes were demonstrated in material from patients with Lyme disease by short-time high-concentrate silver impregnation after treatment with amylase. Removal of mucoid material was essential to visualise Borrelia burgdorferi. Lyme spirochaetes were demonstrated in material from 23 patients with Lyme disease--ery...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-23-3-261
更新日期:1987-05-01 00:00:00
abstract::In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000120
更新日期:2015-09-01 00:00:00
abstract::Three specimens from 111 asymptomatic male partners of infertile couples attending the Department of Urology in Amiens, France, were examined by the PCR COBAS AMPLICOR test (Roche Molecular Diagnostics) for the presence of Chlamydia trachomatis. The specimens analysed were: first void urine (FVU), urine obtained after...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45641-0
更新日期:2004-10-01 00:00:00
abstract::Clostridium piliforme is an obligate intracellular bacterium that causes enterohepatic disease in many animal species. C. piliforme infections are commonly subclinical in laboratory rats and mice, and little is known about host regulation of disease or of the effects of C. piliforme infections on investigations that u...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-6-557
更新日期:2000-06-01 00:00:00
abstract:PURPOSE:The presence of alginate-overproducing (Alg+) strains of Pseudomonas aeruginosa in cystic fibrosis patients is indicative of chronic infection. The Alg+ phenotype is generally due to a mutation in the mucA gene, encoding an innermembrane protein that sequesters AlgT/U, the alginate-specific sigma factor. AlgT/U...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000752
更新日期:2018-08-01 00:00:00
abstract::Human gastric epithelial immortalized GES-1 cells were infected with spiral and coccoid Helicobacter pylori. Scanning electron microscopy was used to determine the ability of the two forms of H. pylori to adhere to GES-1 cells. GES-1 cell apoptosis induced by coccoid and spiral H. pylori was analysed using flow cytome...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46456-0
更新日期:2006-08-01 00:00:00
abstract::Fifty-seven strains of acinetobacter-like organisms were isolated over a period of 26 months from the ears of 55 patients with acute or chronic otitis media, or otitis externa, and one strain was isolated in a survey of 50 normal ears. After comparison with eight reference strains, 32 of the isolates were identified a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-9-3-345
更新日期:1976-08-01 00:00:00
abstract::Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-9-4-451
更新日期:1976-11-01 00:00:00