Isolation and characterisation of an anticryptococcal protein in human cerebrospinal fluid.

Abstract:

:An earlier study reported that human cerebrospinal fluid (CSF) has fungistatic activity for Cryptococcus neoformans. The present study reports that molecular sieve fractionation of concentrated CSF yielded three protein peaks, one of which (p2) had anticryptococcal activity. On a DEAE-Sephacel anion-exchange column the active molecular sieve peak (p2) gave two peaks that contained anticryptococcal activity. The first (DEAE-1) eluted with 0.1 M NaCl and the second (DEAE-2) eluted with 0.2 M NaCl in buffer. Fungistatic activity of DEAE-1 was reversed by FeCl3. Moreover, FeCl3 reversed inhibition of C. neoformans growth by CSF. In contrast, activity of DEAE-2 was not reversed by FeCl3, indicating that inhibition was produced by an iron-independent mechanism. Immunoblot assays showed that transferrin was present in DEAE-1 but not in DEAE-2, whereas albumin was present in DEAE-2 but not in DEAE-1. On NuPAGE, DEAE-1 protein migrated as a single band corresponding to transferrin and DEAE-2 protein gave a single band corresponding to albumin. In control experiments, human serum albumin subjected to the same isolation protocol acquired anticryptococcal activity similar to that of DEAE-2. Therefore, CSF albumin (DEAE-2) activity was associated with the isolation protocol. These data indicate that transferrin, present in or isolated from CSF, sequesters trace amounts of ferric iron, inhibits growth of C. neoformans and acts as an innate defence mechanism.

journal_name

J Med Microbiol

authors

Ahluwalia M,Brummer E,Sridhar S,Singh R,Stevens DA

doi

10.1099/0022-1317-50-1-83

keywords:

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

83-89

issue

1

eissn

0022-2615

issn

1473-5644

journal_volume

50

pub_type

杂志文章
  • DNA identification of the pathogen of candidal aspiration pneumonia induced in the course of oral cancer therapy.

    abstract::Aspiration of oropharyngeal bacteria and fungi is occasionally suspected in patients with pneumonia. A patient with oral carcinoma underwent chemoradioimmunotherapy and, about 4 weeks from the start of the therapy, the patient suffered from severe oral mucositis induced by chemoradiotherapy, and candidal pneumonia was...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45769-0

    authors: Yamamoto T,Ueta E,Kamatani T,Osaki T

    更新日期:2005-05-01 00:00:00

  • Multilocus genetic analysis reveals that the Australian strains of Vibrio cholerae O1 are similar to the pre-seventh pandemic strains of the El Tor biotype.

    abstract::Episodes of cholera stemming from indigenous Vibrio cholerae strains in Australia are mainly associated with environmental sources. In the present study, 10 V. cholerae O1 strains of Australian origin were characterized. All of the strains were serogroup O1 and their conventional phenotypic traits categorized them as ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.004333-0

    authors: Safa A,Bhuiyan NA,Murphy D,Bates J,Nusrin S,Kong RYC,Chongsanguan M,Chaicumpa W,Nair GB

    更新日期:2009-01-01 00:00:00

  • Protection of mice against vaginal colonisation by Mycoplasma pulmonis.

    abstract::Resistance against vaginal colonisation by Mycoplasma pulmonis in strain TO mice after exposure to the mycoplasma was investigated. Eighteen mice from which M. pulmonis had been eliminated from the vagina, either naturally or by antibiotic therapy, were resistant to vaginal recolonisation. Specific antibody was measur...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-3-197

    authors: Taylor-Robinson D,Furr PM

    更新日期:1994-03-01 00:00:00

  • Haemolysin produced by Vibrio cholerae non-O1 is not enterotoxic.

    abstract::Of 28 isolates of Vibrio cholerae non-O1 (10 from diarrhoeal patients and 18 from environmental sources) examined for haemolytic activity and its correlation, if any, with enterotoxic activity, 24 showed haemolysis. The four non-haemolytic isolates showed haemolysis after consecutive passages through rabbit ileal loop...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-1-35

    authors: Singh DV,Shukla BN,Sanyal SC

    更新日期:1996-07-01 00:00:00

  • Aeromonas veronii biovar veronii septicaemia and acute suppurative cholangitis in a patient with hepatitis B.

    abstract::Gram-negative bacilli of the genus Aeromonas are widespread in aquatic environments and can be responsible for human infections. Although Aeromonas extraintestinal and systemic infections have been reported with growing frequency in recent years, Aeromonas septicaemia remains an uncommon finding, often associated with...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05214-0

    authors: Mencacci A,Cenci E,Mazzolla R,Farinelli S,D'Alò F,Vitali M,Bistoni F

    更新日期:2003-08-01 00:00:00

  • Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria.

    abstract::Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combining mycolic acid cleavage products with shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05113-0

    authors: Torkko P,Katila ML,Kontro M

    更新日期:2003-04-01 00:00:00

  • Characterisation of Plesiomonas shigelloides strains that share type-specific antigen with Shigella flexneri 6 and common group 1 antigen with Shigella flexneri spp. and Shigella dysenteriae 1.

    abstract::Three strains of Plesiomonas shigelloides isolated from patients with diarrhoea were agglutinated with Shigella flexneri 6 antiserum in slide and tube tests. All the strains were also agglutinated with a monoclonal antibody to the common group 1 antigen shared between S. flexneri serotypes and S. dysenteriae type 1. F...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-39-3-211

    authors: Albert MJ,Ansaruzzaman M,Qadri F,Hossain A,Kibriya AK,Haider K,Nahar S,Faruque SM,Alam AN

    更新日期:1993-09-01 00:00:00

  • Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995-1998.

    abstract::A total of 3429 isolations of verocytotoxin-producing Escherichia coli O157 (VTEC O157) was confirmed from human sources in England and Wales during the period 1995-1998. The largest annual total was 1087 in 1997. Most infections occurred in the third quarter of each year. The overall rate of infection ranged from 1.2...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-2-135

    authors: Willshaw GA,Cheasty T,Smith HR,O'Brien SJ,Adak GK

    更新日期:2001-02-01 00:00:00

  • Role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of various Candida species.

    abstract::The present study aimed to evaluate the role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of Candida albicans and non-albicans Candida (NAC) spp. Biofilm formation was determined by microtitre plate assay and bright-field and scanning electron microscopy. The matrix ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000747

    authors: Kumari A,Mankotia S,Chaubey B,Luthra M,Singh R

    更新日期:2018-06-01 00:00:00

  • Prevalence of Vibrio cholerae with heat-stable enterotoxin (NAG-ST) and cholera toxin genes; restriction fragment length polymorphisms of NAG-ST genes among V. cholerae O serogroups from a major shrimp production area in Thailand.

    abstract::A total of 148 Vibrio cholerae isolates from a major shrimp production area in Southern Thailand were examined by colony hybridisation for genes encoding heat-stable enterotoxin (NAG-ST) and cholera toxin (CT). Only non-O1 V. cholerae strains were found to harbour NAG-ST (14 of 146) whereas no strains hybridised with ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-3-216

    authors: Dalsgaard A,Serichantalergs O,Shimada T,Sethabutr O,Echeverria P

    更新日期:1995-09-01 00:00:00

  • Prevalence of invasive ability and other virulence-associated characteristics in Providencia alcalifaciens strains isolated in São Paulo, Brazil.

    abstract::Providencia alcalifaciens is an invasive enteric pathogen. The present study determined the prevalence of invasive ability in P. alcalifaciens strains isolated in São Paulo, Brazil, mainly from patients with diarrhoea. Invasion of HeLa cells was found in 17 (42%) of 41 strains studied. Most (88%) of the invasive strai...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-6-459

    authors: Guth BE,Perrella E

    更新日期:1996-12-01 00:00:00

  • Inhibition of streptolysin O by allicin - an active component of garlic.

    abstract::Streptolysin O (SLO) is a potent cytolytic toxin produced by almost all strains of group A streptococci and is considered an important virulence factor for this organism. In this study we investigated the effect of allicin and aqueous garlic extracts on the haemolytic activity of SLO. All tested materials potentially ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019539-0

    authors: Arzanlou M,Bohlooli S

    更新日期:2010-09-01 00:00:00

  • Auxotyping of Neisseria gonorrhoeae isolated in the United Kingdom.

    abstract::One hundred and twenty consecutive isolates of Neisseria gonorrhoeae from the County of Avon, UK, were examined by auxotyping and penicillin susceptibility testing. Requirements for proline (Pro), arginine (Arg), hypoxanthine (Hyp), uracil (Ura) and methionine (Met) were determined with a modified Heckels' medium. Pro...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-16-3-295

    authors: Copley CG,Egglestone SI

    更新日期:1983-08-01 00:00:00

  • Delineation of Stenotrophomonas maltophilia isolates from cystic fibrosis patients by fatty acid methyl ester profiles and matrix-assisted laser desorption/ionization time-of-flight mass spectra using hierarchical cluster analysis and principal component

    abstract::Stenotrophomonas maltophilia is an opportunist multidrug-resistant pathogen that causes a wide range of nosocomial infections. Various cystic fibrosis (CF) centres have reported an increasing prevalence of S. maltophilia colonization/infection among patients with this disease. The purpose of this study was to assess s...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.076950-0

    authors: Vidigal PG,Mosel F,Koehling HL,Mueller KD,Buer J,Rath PM,Steinmann J

    更新日期:2014-12-01 00:00:00

  • Co-agglutination (Co-A) test for circulating antigen in hydatid disease.

    abstract::A co-agglutination (Co-A) test with Staphylococcus aureus (Cowan's strain I) bearing protein A coated with specific hydatid antibodies, was used to demonstrate circulating hydatid antigen in serum for the diagnosis of hydatid disease. The test had a sensitivity of 95% and specificity of 89% in detecting hydatid antige...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-38-6-391

    authors: Shariff M,Parija SC

    更新日期:1993-06-01 00:00:00

  • Cytotoxin detection in Campylobacter jejuni strains of human and animal origin with three tissue culture assay systems.

    abstract::Cytotoxin (CTX) production in 34 human and 22 animal strains of Campylobacter jejuni isolated in Japan and other countries was studied by three assay systems described previously. Furthermore, cholera-like enterotoxin production by these strains was tested by reversed passive latex agglutination (RPLA). CTX titres in ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-5-354

    authors: Misawa N,Ohnishi T,Itoh K,Takahashi E

    更新日期:1995-11-01 00:00:00

  • Genetic and phenotypic characterization of Candida albicans strains isolated from infectious disease patients in Shanghai.

    abstract::Candida albicans, as an opportunistic pathogen, can cause superficial and life-threatening candidiasis in immunocompromised individuals. The formation of surface-associated biofilms and the appearance of drug resistance pose a significant challenge for clinical intervention. In this study, a total of 104 hospital-acqu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.080200-0

    authors: Hu L,Du X,Li T,Song Y,Zai S,Hu X,Zhang X,Li M

    更新日期:2015-01-01 00:00:00

  • Rickettsia rickettsii growth and temperature-inducible protein expression in embryonic tick cell lines.

    abstract::Rickettsia rickettsii has limited adverse effects on its arthropod vector, but causes severe disease in man. To model differences in host-parasite interaction, R. rickettsii growth and protein expression were examined at temperatures reflective of host environment in the tick cell lines DALBE3 and IDE2, the human endo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-10-839

    authors: Policastro PF,Munderloh UG,Fischer ER,Hackstadt T

    更新日期:1997-10-01 00:00:00

  • Cytopathic effects of outer-membrane preparations of enteropathogenic Escherichia coli and co-expression of maltoporin with secretory virulence factor, EspB.

    abstract::Enteropathogenic Escherichia coli (EPEC) is an important aetiological agent of persistent infantile diarrhoea. EPEC pathogenicity is not mediated through known toxins and the role played by outer-membrane proteins (OMPs) in the initial adherence of the bacterium, intimate attachment to epithelial cells and ultimately ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-7-602

    authors: Kumar SS,Sankaran K,Haigh R,Williams PH,Balakrishnan A

    更新日期:2001-07-01 00:00:00

  • Rapid antibiotic sensitivity testing and trimethoprim-mediated filamentation of clinical isolates of the Enterobacteriaceae assayed on a novel porous culture support.

    abstract::A porous inorganic material (Anopore) was employed as a microbial culture and microcolony imaging support. Rapid Anopore-based antibiotic sensitivity testing (AST) methods were developed to assess the growth of clinical isolates, with the primary focus on testing the response of the Enterobacteriaceae to trimethoprim,...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46585-0

    authors: Ingham CJ,van den Ende M,Wever PC,Schneeberger PM

    更新日期:2006-11-01 00:00:00

  • Initial organ localisation of blood-borne Candida albicans in a rat model of disseminated candidosis.

    abstract::The rat was evaluated as an experimental model for disseminated candidosis by quantitating blood clearance and initial organ localisation of 3H-leucine-labelled Candida albicans after intravenous injection into the tail or portal vein. Viable or formalin-killed blastoconidia or viable blastoconidia with germ tubes wer...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-37-4-291

    authors: Katz S,Grosfeld JL,Folkening WJ,Rosenthal RS,Merkel GJ

    更新日期:1992-10-01 00:00:00

  • Nontuberculous mycobacterial and Nocardia infections mimicking pulmonary tuberculosis: a retrospective study of a general hospital patient population in China.

    abstract::Purpose. In this study, we differentiated between tuberculosis (TB) and infections caused by nontuberculous mycobacteria (NTM) or Nocardia in a tertiary general hospital in China. Differences in clinical manifestations and factors between respiratory infections associated with these organisms were also investigated.Me...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000961

    authors: Dong G,Chu P,Guo J,Xie Y,Lu J

    更新日期:2020-09-01 00:00:00

  • Plasmid-mediated resistance to gentamicin in Staphylococcus aureus: the involvement of a transposon.

    abstract::Resistance to gentamicin, tobramycin and kanamycin (GmrTmrKmr) in strains of Staphylococcus aureus isolated from clinical sources in Australia is mediated by a 4.7 kb transposable element, designated Tn4001. A 2.5 kb HindIII fragment which maps symmetrically within Tn4001, and encompasses the aminoglycoside-resistance...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-23-2-101

    authors: Lyon BR,Gillespie MT,Byrne ME,May JW,Skurray RA

    更新日期:1987-03-01 00:00:00

  • Depletion of lymphocytes, but not neutrophils, via apoptosis in a murine model of Vibrio vulnificus infection.

    abstract::Vibrio vulnificus causes severe sepsis in humans. There are several reports about the relationship between host immunity and bacterial growth in V. vulnificus infection. However, the effect on leukocytes of V. vulnificus infection in vivo has not been elucidated. A murine model of V. vulnificus infection was used to i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45861-0

    authors: Kashimoto T,Ueno S,Hayashi H,Hanajima M,Yoshioka K,Yoshida K,Mutoh K,Susa N

    更新日期:2005-01-01 00:00:00

  • Isolation of a gastric campylobacter-like organism from the stomach of four rhesus monkeys, and identification as Campylobacter pylori.

    abstract::Campylobacter-like organisms, isolated from the gastric antrum of Rhesus monkeys, were compared with Campylobacter jejuni and C. pylori. They were similar to C. pylori by light microscopy, in ultrastructural morphology, in enzymic, fatty-acid-methyl-ester, and protein-profile analysis, and in antigenic reactivity with...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-27-1-41

    authors: Newell DG,Hudson MJ,Baskerville A

    更新日期:1988-09-01 00:00:00

  • Evaluation of the Sofia Streptococcus pneumoniae FIA test for the detection of S. pneumoniae antigen in urine.

    abstract::In this study, we evaluated the Sofia Streptococcus pneumoniae FIA test (Quidel Corporation, San Diego, CA, USA), a new immunofluorescence-based lateral flow test for the qualitative detection of S. pneumoniae antigen in urine or cerebrospinal fluid specimens. The analyses of 100 non-concentrated urine samples (includ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000877

    authors: Euser SM,Badoux P,Kracht-Kosten L,Yzerman EPF

    更新日期:2018-12-01 00:00:00

  • Mycobacterium tuberculosis co-existence with humans: making an imprint on the macrophage P2X(7) receptor gene?

    abstract::The development of tuberculosis (TB) infection in humans depends on the mycobacterial strain and the human host, and is multigenically controlled in both. ATP ligation of P2X(7) receptors expressed on human macrophages infected with mycobacteria induces cell death and subsequent loss of intracellular bacterial viabili...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47455-0

    authors: Mokrousov I,Sapozhnikova N,Narvskaya O

    更新日期:2008-05-01 00:00:00

  • Pseudomonas aeruginosa infection and vaccination in the rat.

    abstract::A large molecular-weight fraction of Pseudomonas aeruginosa culture filtrate protected rats and mice against a lethal infection with a heterologous serotype, and to some extent against Escherichia coli and Listeria monocytogenes. The active components were obtained from cultures grown for several days in a simple synt...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-13-2-201

    authors: Kostiala AA

    更新日期:1980-05-01 00:00:00

  • Cross-reactions in cell-mediated immunity to Salmonella causing enteric fever.

    abstract::Cross-reactivity in a delayed-type hypersensitivity (DH) response was studied in mice immunised with live Salmonella typhi, S. paratyphi A and S. paratyphi B. Extensive cross-reactions outside the serogroup limits were observed. The ability of DH cross-reacting and non-cross-reacting sonicates to generate activated ma...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-3-233

    authors: Tiwari H,Kamat RS

    更新日期:1986-05-01 00:00:00

  • Evaluation of antigenic variations between two virulent toxoplasma strains.

    abstract::Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies o...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46712-0

    authors: Delibas SB,Ertabaklar H,Ertug S

    更新日期:2006-10-01 00:00:00