Prevalence of invasive ability and other virulence-associated characteristics in Providencia alcalifaciens strains isolated in São Paulo, Brazil.

abstract：:A sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody coupled to the solid phase was evaluated for the detection of brucella antigen in serum samples. Under optimum conditions, 100 brucella cells/well or 10(5) fg/ml of lipopolysaccharide (LPS) were detected in spiked specimens. The standardised...

journal_title：Journal of medical microbiology

authors： Al-Shamahy HA,Wright SG

更新日期：1998-02-01 00:00:00

abstract：:A collection of Enterococcus faecalis strains from clinical isolates, healthy individuals and the environment was screened for the presence of virulence factor genes, such as those for collagen-binding protein (ace), endocarditis antigen (efaA), haemolysin activator (cylA), gelatinase (gelE), aggregation substances (a...

journal_title：Journal of medical microbiology

authors： Creti R,Imperi M,Bertuccini L,Fabretti F,Orefici G,Di Rosa R,Baldassarri L

更新日期：2004-01-01 00:00:00

abstract：:The purpose of this study was to compare the frequency of recovery of aerobic and anaerobic organisms with interfering capability against group A beta-haemolytic streptococci (GABHS) and beta-lactamase-producing bacteria (BLPB) from the tonsils of GABHS carriers and non-carriers. The presence of aerobic and anaerobic ...

journal_title：Journal of medical microbiology

authors： Brook I,Gober AE

更新日期：2006-12-01 00:00:00

abstract：:To study the influence of normal associated microbiota on systemic immunological responses during experimental Chagas' disease, germ-free and conventional NIH Swiss mice were infected with Y strain of Trypanosoma cruzi. Although no statistical differences in mortality and parasitaemia were found, conventional mice sho...

journal_title：Journal of medical microbiology

authors： Duarte R,Silva AM,Vieira LQ,Afonso LCC,Nicoli JR

更新日期：2004-08-01 00:00:00

abstract：:Sequence variations located at the signal sequence and mid-region within the vacA gene, the 3'-end of the cagA gene, the indel motifs at the 3'-end of the cag pathogenicity island and the regions upstream of the vacA and ribA genes were determined by PCR in 19 paired antral or antrum and corpus Helicobacter pylori iso...

journal_title：Journal of medical microbiology

authors： Carroll IM,Ahmed N,Beesley SM,Khan AA,Ghousunnissa S,Moráin CAÓ,Habibullah CM,Smyth CJ

更新日期：2004-07-01 00:00:00

abstract：:The ability of Mycobacterium leprae to specifically bind alpha2-laminins of Schwann cells has been described recently as being an important property of the leprosy bacillus, which could explain the neural tropism of M. leprae. Therefore, the extent of the expression of alpha2-laminin-binding properties among mycobacte...

journal_title：Journal of medical microbiology

authors： Marques MA,Ant nio VL,Sarno EN,Brennan PJ,Pessolani MC

更新日期：2001-01-01 00:00:00

abstract：:Large-scale monitoring of resistance to 14 antimicrobial agents was performed using 103 Proteus mirabilis strains isolated from dogs in Japan. Resistant strains were analysed to identify their resistance mechanisms. Rates of resistance to chloramphenicol, streptomycin, enrofloxacin, trimethoprim/sulfamethoxazole, kana...

journal_title：Journal of medical microbiology

authors： Harada K,Niina A,Shimizu T,Mukai Y,Kuwajima K,Miyamoto T,Kataoka Y

更新日期：2014-11-01 00:00:00

abstract：:Five Bordetella pertussis strains of phase I were grown in conventional casamino-acid medium and in media modified by adding high concentrations of MgSO4 or nicotinic acid. Cells grown in high-magnesium media (in the C-mode) had only about 4% of the protective antigen (PA) and 6% of the histamine-sensitising factor (H...

journal_title：Journal of medical microbiology

authors： Wardlaw AC,Parton R,Hooker MJ

更新日期：1976-02-01 00:00:00

abstract：:The susceptibility of chicks to enteritis caused by Campylobacter jejuni was studied. Three-day-old chicks did not develop enteritis after oral infection but chicks infected within 12 h of hatching developed gastroenteritis. The incubation period correlated with the inoculum size. Initially, infected chicks developed ...

journal_title：Journal of medical microbiology

authors： Welkos SL

更新日期：1984-10-01 00:00:00

abstract：:Following characterisation by phenotypic tests and amplified ribosomal DNA restriction analysis (ARDRA), 50 tetracycline-resistant (MIC > or = 16 mg/L) Acinetobacter strains from clinical (n = 35) and aquatic (n = 15) samples were analysed by PCR for tetracycline resistance (Tet) determinants of classes A-E. All the c...

journal_title：Journal of medical microbiology

authors： Guardabassi L,Dijkshoorn L,Collard JM,Olsen JE,Dalsgaard A

更新日期：2000-10-01 00:00:00

abstract：:The role of Bacteroides fragilis in the pathogenesis of acute appendicitis was studied in 135 patients in four patient groups: normal (17); phlegmonous appendicitis (17); gangrenous appendicitis (75); and septic complications of appendicitis (26). Aerobic and anaerobic bacteria were isolated from all groups and member...

journal_title：Journal of medical microbiology

authors： Elhag KM,Alwan MH,Al-Adnani MS,Sherif RA

更新日期：1986-05-01 00:00:00

abstract：:To clarify further the role of chemotaxis in Helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined. Human plasma, after dilution to 1 % (v/v) with buffer, was found to be a chem...

journal_title：Journal of medical microbiology

authors： Worku ML,Karim QN,Spencer J,Sidebotham RL

更新日期：2004-08-01 00:00:00

abstract：:Here, we describe the first report of post-operative endophthalmitis due to Comamonas testosteroni in an elderly diabetic patient after complicated cataract surgery. The isolate was identified by using Mini API strips. The patient was successfully treated with intravitreal ceftazidime and oral ciprofloxacin. ...

journal_title：Journal of medical microbiology

authors： Reddy AK,Murthy SI,Jalali S,Gopinathan U

更新日期：2009-03-01 00:00:00

abstract：:Rickettsia rickettsii has limited adverse effects on its arthropod vector, but causes severe disease in man. To model differences in host-parasite interaction, R. rickettsii growth and protein expression were examined at temperatures reflective of host environment in the tick cell lines DALBE3 and IDE2, the human endo...

journal_title：Journal of medical microbiology

authors： Policastro PF,Munderloh UG,Fischer ER,Hackstadt T

更新日期：1997-10-01 00:00:00

abstract：:This study analysed the characteristics and genetic similarity of recent Klebsiella pneumoniae carbapenemase (KPC-2)-producing Klebsiella pneumoniae isolates from Korea. Recent laboratory surveillance detected an increase in carbapenemase-producing Enterobacteriaceae in Korea. A total of 6 KPC-2-producing K. pneumonia...

journal_title：Journal of medical microbiology

authors： Yoo JS,Kim HM,Yoo JI,Yang JW,Kim HS,Chung GT,Lee YS

更新日期：2013-09-01 00:00:00

abstract：:In a hospital-based study, stool samples from 2095 patients of all ages were examined for different fungal, protozoal and bacterial enteropathogens over a period of 2 years (July 1994-June 1996). Cryptosporidium was detected in 151 specimens (7.2%) and was the third commonest pathogen found. The highest prevalence of ...

journal_title：Journal of medical microbiology

authors： Nath G,Choudhury A,Shukla BN,Singh TB,Reddy DCS

更新日期：1999-06-01 00:00:00

abstract：:Actinobacillus actinomycetemcomitans is considered to be an aetiological agent in various forms of periodontitis, with serotype b-specific carbohydrate being the immunodominant antigen of A. actinomycetemcomitans Y4 in high-responder patients. Lipopolysaccharide (LPS) of the organism may also be an important antigen. ...

journal_title：Journal of medical microbiology

authors： Nakashima K,Usui C,Koseki T,Nishihara T,Ishikawa I

更新日期：1998-07-01 00:00:00

abstract：:To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...

journal_title：Journal of medical microbiology

authors： Sugawara I,Yamada H,Kazumi Y,Doi N,Otomo K,Aoki T,Mizuno S,Udagawa T,Tagawa Y,Iwakura Y

更新日期：1998-10-01 00:00:00

abstract：:As well as conventional methods such as immunodiffusion, ELISA, or agglutination for the detection of toxin production in Staphylococcus aureus, amplification techniques like PCR allow a very sensitive and specific identification of the genes responsible for enterotoxin B and C, and TSST-1 production. These toxins mig...

journal_title：Journal of medical microbiology

authors： Schmitz FJ,Steiert M,Hofmann B,Verhoef J,Hadding U,Heinz HP,Köhrer K

更新日期：1998-04-01 00:00:00

abstract：:Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...

journal_title：Journal of medical microbiology

authors： Barketi-Klai A,Hoys S,Lambert-Bordes S,Collignon A,Kansau I

更新日期：2011-08-01 00:00:00

abstract：:A longitudinal analysis was carried out of the colonization by four potential respiratory pathogens - Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus - in 165 healthy children (aged 3-7 years) attending three kindergartens and 417 healthy children (aged 7-10 years) att...

journal_title：Journal of medical microbiology

authors： Bae S,Yu JY,Lee K,Lee S,Park B,Kang Y

更新日期：2012-05-01 00:00:00

abstract：:Bacteriophages in different stages of maturation were found in thin sections of a clinical isolate of Helicobacter (Campylobacter) pylori. Mature phage heads measured 70 x 60 nm and the tail at least 120 nm. Lysogeny was maintained during subculture on blood agar for more than 3 months after isolation from a gastric b...

journal_title：Journal of medical microbiology

authors： Schmid EN,von Recklinghausen G,Ansorg R

更新日期：1990-06-01 00:00:00

abstract：:Cutaneous leishmaniasis (CL), a neglected tropical disease, is reported to be prevalent in tribal villages located in the Agasthyamala Biosphere Reserve forests of Western Ghats, Kerala state, India. We carried out an investigation to characterize the species of Leishmania parasites involved in these infections preval...

journal_title：Journal of medical microbiology

authors： Kumar NP,Srinivasan R,Anish TS,Nandakumar G,Jambulingam P

更新日期：2015-02-01 00:00:00

abstract：:Introduction. Rapid identification of the causative agent of sepsis is crucial for patient outcomes.Aim. The Sepsityper sample preparation method enables direct microbial identification of positive blood culture samples via matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS).Hyp...

journal_title：Journal of medical microbiology

authors： Cordovana M,Zignoli A,Ambretti S

更新日期：2020-12-01 00:00:00

abstract：:The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five ...

journal_title：Journal of medical microbiology

authors： Monstein HJ,Tiveljung A,Kraft CH,Borch K,Jonasson J

更新日期：2000-09-01 00:00:00

abstract：:India contributes heavily to the global burden of visceral leishmaniasis (VL, kala-azar) and human immunodeficiency virus (HIV)/AIDS. The prevalence of HIV seropositivity in VL patients at a tertiary care centre in northern India, as observed during a prospective study over a period of 2 years, is presented. Of the 10...

journal_title：Journal of medical microbiology

authors： Mathur P,Samantaray JC,Vajpayee M,Samanta P

更新日期：2006-07-01 00:00:00

abstract：:An evaluation of five ELISA methods for the diagnosis of acute toxoplasmosis was undertaken by comparing a laboratory-produced ELISA employing two recombinant Toxoplasma gondii polypeptides as antigen with a laboratory-produced ELISA and three commercially available ELISAs employing traditional parasite antigen prepar...

journal_title：Journal of medical microbiology

authors： Johnson AM,Roberts H,Tenter AM

更新日期：1992-12-01 00:00:00

abstract：:Eighty-one bronchoalveolar lavage (BAL) specimens obtained from 26 HIV-infected, 45 non-HIV immunosuppressed and 10 immunocompetent patients with primary pulmonary diseases were analysed for the presence of Pneumocystis carinii by staining and by P. carinii 5S rDNA determined by PCR. P. carinii was observed by stainin...

journal_title：Journal of medical microbiology

authors： Takahashi T,Goto M,Endo T,Nakamura T,Yusa N,Sato N,Iwamoto A

更新日期：2002-07-01 00:00:00

abstract：:Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differenti...

journal_title：Journal of medical microbiology

authors： Ramis IB,Cnockaert M,Von Groll A,Mathys V,Simon A,Tortoli E,Palomino JC,Almeida da Silva PE,Vandamme P,Andre E,Martin A

更新日期：2015-03-01 00:00:00

abstract：:Pseudomonas aeruginosa produces multiple virulence factors and causes different types of infections. Previous clinical studies identified P. aeruginosa isolates that lack individual virulence factors. However, the impact of losing several virulence factors simultaneously on the in vivo virulence of P. aeruginosa is no...

journal_title：Journal of medical microbiology

authors： Schaber JA,Carty NL,McDonald NA,Graham ED,Cheluvappa R,Griswold JA,Hamood AN

更新日期：2004-09-01 00:00:00