Abstract:
:In this study, we evaluated the Sofia Streptococcus pneumoniae FIA test (Quidel Corporation, San Diego, CA, USA), a new immunofluorescence-based lateral flow test for the qualitative detection of S. pneumoniae antigen in urine or cerebrospinal fluid specimens. The analyses of 100 non-concentrated urine samples (including 50 samples from S. pneumoniae cases) showed a sensitivity and specificity (95 % CI) of, respectively, 66.0 % (52.2-77.6) and 100.0 % (92.9-100.0) for the Sofia test, and 62.0 % (48.2-74.1) and 98.0 % (89.5-99.7) for the BinaxNOW SPN Antigen Card. There were no significant differences in sensitivity and specificity between the tests (McNemar's tests, P=0.625 and P=1.000). In conclusion, this study indicates that the Streptococcus pneumoniae FIA test shows similar sensitivity and specificity rates compared to the BinaxNOW SPN Antigen Card.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Euser SM,Badoux P,Kracht-Kosten L,Yzerman EPFdoi
10.1099/jmm.0.000877subject
Has Abstractpub_date
2018-12-01 00:00:00pages
1743-1746issue
12eissn
0022-2615issn
1473-5644journal_volume
67pub_type
杂志文章abstract:PURPOSE:Catheter-related bloodstream infections (CRBSIs) are among the most common hospital-acquired infections. We aimed to survey methicillin resistance, biofilm production and susceptibility to vancomycin, linezolid and other antibiotics for staphylococci isolated from CRBSIs. METHODOLOGY:Fifty-eight isolates [20 S...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000490
更新日期:2017-06-01 00:00:00
abstract::Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enter...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-7-548
更新日期:2002-07-01 00:00:00
abstract::The production of toxins A and B by Clostridium difficile was greatly enhanced under biotin-limited conditions, in which a 140-kDa protein was expressed strongly. Gene cloning revealed that this protein was a homologue of formylglycinamidine ribonucleotide synthetase (FGAM synthetase, EC 6.3.5.3), which is known as Pu...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-1-34
更新日期:2002-01-01 00:00:00
abstract::Effects on growth and toxin A production of sub-MIC concentrations of six different antibiotics were investigated in three strains of Clostridium difficile: reference strain NCTC 11223, a fully sequenced strain (630) and a locally endemic isolate (strain 338a). The antibiotics chosen for investigation were the agents ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05387-0
更新日期:2003-12-01 00:00:00
abstract::The determinant responsible for the ability of Bacteroides spp. to inhibit polymorph phagocytic killing of aerobic organisms has not yet been identified. Therefore, the roles of lipopolysaccharide and capsular polysaccharide of B. fragilis were investigated. Serum-resistant and serum-sensitive strains of Proteus mirab...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-17-3-259
更新日期:1984-06-01 00:00:00
abstract::Acinetobacter junii is a rare human pathogen associated with bacteraemia in neonates and paediatric oncology patients. We present a case of A. junii causing bacteraemia in an adult transplant patient with leukaemia. The correct identification of Acinetobacter species can highlight the clinical significance of the diff...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.024596-0
更新日期:2011-03-01 00:00:00
abstract::The advent of next-generation sequencing technology has revolutionized the field of prokaryotic genetics and genomics by allowing interrogation of entire genomes, transcriptomes and global transcription factor binding profiles. As more studies employing these techniques have been performed, the state of the art regard...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.001135
更新日期:2020-02-01 00:00:00
abstract:PURPOSE:To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients. METHODOLOGY:Clinical and demographic data were registered prospectively. Phylogenetic background of K. pneumoniae isolates was analysed by PCR melting pro...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000942
更新日期:2019-03-01 00:00:00
abstract::CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an efflux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug res...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46650-0
更新日期:2006-10-01 00:00:00
abstract::A novel direct detection system has been developed for eight staphylococcal enterotoxin (SE)-encoding genes (sea, seb, sec, sed, see, seg, seh and sei) in milk. Specific detection by real-time PCR was successful for all SE-encoding genes in the reference strains. Furthermore, a novel DNA-preparation method with good r...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46027-0
更新日期:2006-03-01 00:00:00
abstract::The microtechnique of Galton et al. (1965) for the routine screening of blood samples for leptospiral antibodies was used to examine more than 8000 serum samples from animals that had aborted and from herds in which leptospiral antibodies had previously been demonstrated. The method gave consistently accurate results....
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-11-3-355
更新日期:1978-08-01 00:00:00
abstract::The move towards pathology networks and hub-and-spoke models of medical laboratory service provision has significantly changed the flow of samples, and the impact of results on patients, over recent years. At the same time advances in technology, including rapid, simple to use molecular platforms, are changing the way...
journal_title:Journal of medical microbiology
pub_type: 信件
doi:10.1099/jmm.0.000919
更新日期:2019-03-01 00:00:00
abstract::Introduction.Candida auris is a pathogenic yeast that mainly affects immunosuppressed patients and those with implanted medical devices. This pathogen also displays elevated resistance to common antifungals and high survival and spreading capacities. Since no antifungal breakpoints have yet been defined for this patho...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001036
更新日期:2019-09-01 00:00:00
abstract::Escherichia coli isolates (n=2629) were collected between 1996 and 2000 from 2100 Thai children less than 12 years of age with acute diarrhoea. Enterotoxigenic (ETEC), enteroinvasive (EIEC), Shiga-toxin-producing (STEC), enteropathogenic (EPEC) and enteroaggregative (EAEC) E. coli were identified by their virulence ma...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05413-0
更新日期:2004-03-01 00:00:00
abstract::The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.049429-0
更新日期:2012-12-01 00:00:00
abstract::Acinetobacter baumannii is an opportunist nosocomial pathogen of world-wide importance and produces severe infections in immunocompromised patients. However, the virulence factors contributing to its pathogenic properties are not well known. The effect of normal human serum against 18 clinical isolates of the most pre...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-11-1047
更新日期:2000-11-01 00:00:00
abstract::The potential of incorporating a real-time PCR for amplification and detection of 16S rRNA gene signatures directly from clinical samples was assessed as a tool for diagnostics. Universal PCR primers spanning short variable regions (~500 bp) were optimized for real-time PCR and tested in comparison with a longer fragm...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.041764-0
更新日期:2012-05-01 00:00:00
abstract::To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-10-871
更新日期:1998-10-01 00:00:00
abstract::During 2003, Vibrio cholerae O1 Ogawa was the predominant serotype among diarrhoeal patients admitted to different hospitals in India. With the exception of 3 strains from Kolkata, none of 172 strains examined exhibited resistance to tetracycline, but 45.7 % showed reduced susceptibility to ciprofloxacin. Extensive mo...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46982-0
更新日期:2007-06-01 00:00:00
abstract::Micro-organisms isolated from the oral cavity may translocate to the lower airways during mechanical ventilation (MV) leading to ventilator-associated pneumonia (VAP). Changes within the dental plaque microbiome during MV have been documented previously, primarily using culture-based techniques. The aim of this study ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000212
更新日期:2016-02-01 00:00:00
abstract::We report a third case of Mycobacterium conceptionense infection, which was found in a 50-year-old female following face rejuvenation surgery with fat grafting. The pathogen was identified using 16S rRNA gene and rpoB gene sequences. The growing diversity of non-tuberculosis mycobacterial species causing human infecti...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.024554-0
更新日期:2011-03-01 00:00:00
abstract:INTRODUCTION:Idiopathic pulmonary fibrosis (IPF) is a chronic and ultimately fatal lung disease. One of the risk factors involved in the acquisition of IPF is viral infections, especially respiratory viruses. In the present study, we investigated the detection of respiratory viruses and the possible relationship betwee...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000628
更新日期:2017-11-01 00:00:00
abstract::The influence of six immunosuppressive agents on the occurrence of endogenous bacteraemia in mice was evaluated. The mortality rates in conventional ddY mice given cyclophosphamide (CY), fluorouracil (5-FU), methotrexate (MTX), cisplatin (CDDP) or FK-506 intraperitoneally, or dexamethasone (DXM) subcutaneously were 70...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-42-3-181
更新日期:1995-03-01 00:00:00
abstract::A total of 287 Acinetobacter isolates belonging to DNA groups 2 (A. baumannii) and 13TU was collected consecutively from 46 hospitals and typed by randomly amplified polymorphic DNA fingerprinting with primers DAF-4 and ERIC-2. With a similarity coefficient of >/=72% as a cut-off value, 37 clusters of genotypically si...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-12-1107
更新日期:2002-12-01 00:00:00
abstract:PURPOSE:The presence of alginate-overproducing (Alg+) strains of Pseudomonas aeruginosa in cystic fibrosis patients is indicative of chronic infection. The Alg+ phenotype is generally due to a mutation in the mucA gene, encoding an innermembrane protein that sequesters AlgT/U, the alginate-specific sigma factor. AlgT/U...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000752
更新日期:2018-08-01 00:00:00
abstract::Of 2458 isolates of Haemophilus influenzae examined in a recent British survey, 42 were resistant to chloramphenicol. Two resistant isolates were of type b and 40 were non-capsulate. Spectrophotometric assay showed that all the resistant isolates produced chloramphenicol acetyltransferase (CAT). CAT activity did not i...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-27-2-89
更新日期:1988-10-01 00:00:00
abstract::Neisseria gonorrhoeae expresses receptors for both lactoferrin (LF) and transferrin (TF). To determine whether qualitative or quantitative changes in these receptors, or both, correlate with the pattern of disease due to N. gonorrhoeae, a dot binding assay with whole cells was used to measure the absolute receptor lev...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-28-3-199
更新日期:1989-03-01 00:00:00
abstract::The closely related Clostridium novyi and Clostridium botulinum types C and D are of current interest because of their association with serious infections in injecting drug users (C. novyi type A) and equine and feline dysautonomias (C. botulinum types C/D). The species are defined by the major toxins they produce: th...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46802-0
更新日期:2007-02-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that poses a threat in clinical settings due to its intrinsic and acquired resistance to a wide spectrum of antibiotics. Additionally, the presence of a subpopulation of cells surviving high concentrations of antibiotics, called persisters, makes it virtually impossi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019703-0
更新日期:2011-03-01 00:00:00
abstract::We describe the case of a graft versus host disease (GvHD) patient, in whom Hafnia alvei was cultured as a single organism, and at high bacterial counts from stool samples, from the onset of the disease until its resolution. This case is a further example of the contentious role of this species in causing human intest...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.2008/001164-0
更新日期:2008-09-01 00:00:00