Identification of Burkholderia cepacia complex pathogens by rapid-cycle PCR with fluorescent hybridization probes.

abstract：:In 2002, a Scottish bat conservationist developed a rabies-like disease and subsequently died. This was caused by infection with European bat lyssavirus 2 (EBLV-2), a virus closely related to Rabies virus (RABV). The source of this infection and the means of transmission have not yet been confirmed. In this study, the...

journal_title：Journal of medical microbiology

authors： Johnson N,Phillpotts R,Fooks AR

更新日期：2006-06-01 00:00:00

abstract：:The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...

journal_title：Journal of medical microbiology

authors： Desai D,Ellington MJ,Arnold C,Desai M

更新日期：2012-12-01 00:00:00

abstract：:Early reports of Candida krusei in man describe the organism as a transient, infrequent isolate of minor clinical significance inhabiting the mucosal surfaces. More recently it has emerged as a notable pathogen with a spectrum of clinical manifestations such as fungaemia, endophthalmitis, arthritis and endocarditis, m...

journal_title：Journal of medical microbiology

authors： Samaranayake YH,Samaranayake LP

更新日期：1994-11-01 00:00:00

abstract：:Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However, there are few published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD are essential to inform policy regarding pneumococcal conj...

journal_title：Journal of medical microbiology

authors： Jefferies JMC,Tee WSN,Clarke SC

更新日期：2011-06-01 00:00:00

abstract：:Molecular typing is an important tool for control and prevention of infection. A suitable molecular typing method for epidemiological investigation must be easy to perform, highly reproducible, inexpensive, rapid and easy to interpret. In this study, two molecular typing methods including the conventional PCR-sequenci...

journal_title：Journal of medical microbiology

authors： Fasihi Y,Fooladi S,Mohammadi MA,Emaneini M,Kalantar-Neyestanaki D

更新日期：2017-09-01 00:00:00

abstract：:Of 28 isolates of Vibrio cholerae non-O1 (10 from diarrhoeal patients and 18 from environmental sources) examined for haemolytic activity and its correlation, if any, with enterotoxic activity, 24 showed haemolysis. The four non-haemolytic isolates showed haemolysis after consecutive passages through rabbit ileal loop...

journal_title：Journal of medical microbiology

authors： Singh DV,Shukla BN,Sanyal SC

更新日期：1996-07-01 00:00:00

abstract：:Production of a meningococcal vaccine capable of generating long-lasting immunity in all age groups is still a high priority worldwide. Iron-regulated outer-membrane proteins have attracted considerable attention in recent years and it has become increasingly evident that the meningococcal transferrin-binding proteins...

journal_title：Journal of medical microbiology

authors： Ala'Aldeen DA

更新日期：1996-04-01 00:00:00

abstract：:In order to develop a model for the study of gastric spiral bacteria, and based on the observation that Wistar rats do not carry urease-positive spiral bacteria in their gastric mucosa, mucus from a pig naturally colonised by 'Gastrospirillum suis' (an organism with I6S rDNA 99.5% similar to that of 'G. hominis' type ...

journal_title：Journal of medical microbiology

authors： Mendes EN,Queiroz DM,Coimbra RS,Moura SB,Barbosa AJ,Rocha GA

更新日期：1996-02-01 00:00:00

abstract：:The present study aimed to evaluate the role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of Candida albicans and non-albicans Candida (NAC) spp. Biofilm formation was determined by microtitre plate assay and bright-field and scanning electron microscopy. The matrix ...

journal_title：Journal of medical microbiology

authors： Kumari A,Mankotia S,Chaubey B,Luthra M,Singh R

更新日期：2018-06-01 00:00:00

abstract：:Meticillin-resistant Staphylococcus aureus (MRSA) was unexpectedly isolated from a pig used for streptozotocin-induced diabetes research. To investigate the possible source of the MRSA isolate, nasal swabs were obtained from the animal herd, and from animal holding rooms, and veterinary and research staff involved in ...

journal_title：Journal of medical microbiology

authors： Sergio DMB,Koh TH,Hsu LY,Ogden BE,Goh ALH,Chow PKH

更新日期：2007-08-01 00:00:00

abstract：:Large-scale monitoring of resistance to 14 antimicrobial agents was performed using 103 Proteus mirabilis strains isolated from dogs in Japan. Resistant strains were analysed to identify their resistance mechanisms. Rates of resistance to chloramphenicol, streptomycin, enrofloxacin, trimethoprim/sulfamethoxazole, kana...

journal_title：Journal of medical microbiology

authors： Harada K,Niina A,Shimizu T,Mukai Y,Kuwajima K,Miyamoto T,Kataoka Y

更新日期：2014-11-01 00:00:00

abstract：:Clostridium perfringens type A produces an enterotoxin that induces diarrhoea experimentally in man and animals. The enterotoxin causes increased membrane permeability in susceptible cells which is thought to be due to pore formation in the host cell membrane. The effect of purified C. perfringens enterotoxin on intac...

journal_title：Journal of medical microbiology

authors： Hardy SP,Denmead M,Parekh N,Granum PE

更新日期：1999-03-01 00:00:00

abstract：:A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate m...

journal_title：Journal of medical microbiology

authors： Slack MP,Wheldon DB

更新日期：1978-11-01 00:00:00

abstract：:Coccidioidal pericarditis is a condition found in approximately 1-5% of patients infected by Coccidioides species. It is associated with widely diverse clinical symptoms. This paper reports a case of coccidioidal pericarditis diagnosed by an in-house Coccidioides posadasii antigen and confirmed with mycological and mo...

journal_title：Journal of medical microbiology

authors： Brilhante RSN,Cordeiro RA,Rocha MFG,Fechine MAB,Furtado FM,Nagao-Dias AT,Camargo ZP,Sidrim JJC

更新日期：2008-10-01 00:00:00

abstract：PURPOSE:Conventional laboratory detection methods for gastrointestinal parasites are time consuming, require considerable technical expertise and may suffer from poor analytical sensitivity. This study sought to evaluate the automated BD MAX Enteric Parasite Panel (EPP) for the detection of Cryptosporidium parvum/homin...

journal_title：Journal of medical microbiology

authors： Perry MD,Corden SA,Lewis White P

更新日期：2017-08-01 00:00:00

abstract：:Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the i...

journal_title：Journal of medical microbiology

authors： Popoff MR,Ravisse P

更新日期：1985-06-01 00:00:00

abstract：:Nosocomial infections with enterococci are an increasing problem in modern medical practice due to the development of resistance to a wide range of antibiotics, including the glycopeptides vancomycin and teicoplanin. An increasing number of vancomycin-resistant enterococci (VRE) have been cultured from clinical specim...

journal_title：Journal of medical microbiology

authors： Mackenzie CR,Hucke C,MÜller D,Seidel K,Takikawa O,DÄubener W

更新日期：1999-10-01 00:00:00

abstract：:The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic pla...

journal_title：Journal of medical microbiology

authors： Hawkey PM,Bennett PM,Hawkey CA

更新日期：1984-10-01 00:00:00

abstract：:Brachyspira pilosicoli is a potentially zoonotic anaerobic intestinal spirochaete that is one of several species causing avian intestinal spirochaetosis. The aim of this study was to develop a reproducible model of infection in point-of-lay chickens and compare the virulence of two strains of B. pilosicoli in a model ...

journal_title：Journal of medical microbiology

authors： Mappley LJ,Tchórzewska MA,Nunez A,Woodward MJ,La Ragione RM

更新日期：2013-02-01 00:00:00

abstract：:The distribution of K1, K5 and O antigens was examined in 500 clinical strains of Escherichia coli. Of 400 strains from urine, 52% belonged to serogroups O1, O2, O4, O6, O7, O8, O9, O18, O25 and O75; 34% were non-typable (NT) and 14% were autoagglutinable (AA). Antigen K1 was carried by 17% of these strains, and K5 by...

journal_title：Journal of medical microbiology

authors： Devine DA,Robinson L,Roberts AP

更新日期：1989-12-01 00:00:00

abstract：:Iliopsoas abscess is usually secondary to the spread of infection from a contiguous focus. Primary disease is uncommon, except in children where Staphylococcus aureus is the main pathogen. We report a 60-year-old woman who developed a primary iliopsoas abscess as a result of haematogenous spread of Capnocytophaga sput...

journal_title：Journal of medical microbiology

authors： Chan JFW,Wong SSY,Leung SSM,Li IWS,To KKW,Cheng VCC,Yuen KY

更新日期：2010-11-01 00:00:00

abstract：:Antibody (AMI) and cell-mediated (CMI) immunity to rubella virus (RV) were evaluated in healthy adolescent males (n = 11) and females (n = 28) undergoing routine reimmunisation with RA27/3 strain RV as a component of measles-mumps-rubella (MMR) vaccine. Blood samples were collected just before and at 2, 4 and 10 weeks...

journal_title：Journal of medical microbiology

authors： Mitchell LA

更新日期：1999-12-01 00:00:00

abstract：:A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched...

journal_title：Journal of medical microbiology

authors： Singh N,Prasad P,Singh LC,Das B,Rastogi S

更新日期：2016-06-01 00:00:00

abstract：PURPOSE:The presence of alginate-overproducing (Alg+) strains of Pseudomonas aeruginosa in cystic fibrosis patients is indicative of chronic infection. The Alg+ phenotype is generally due to a mutation in the mucA gene, encoding an innermembrane protein that sequesters AlgT/U, the alginate-specific sigma factor. AlgT/U...

journal_title：Journal of medical microbiology

authors： Pandey S,Delgado C,Kumari H,Florez L,Mathee K

更新日期：2018-08-01 00:00:00

abstract：:Erythrocyte-sensitising antigens (AE) were prepared from Vibrio cholerae serotypes, from EL-Tor vibrio, Escherichia coli and Salmonella enteritidis by digesting the organisms with NaOH followed by precipitation with alcohol. When AE was used in indirect haemagglutination (IHA) tests, the results in a number of cases w...

journal_title：Journal of medical microbiology

authors： Garabedian GA,Barsumian EL,Malakian A

更新日期：1975-02-01 00:00:00

abstract：:The closely related Clostridium novyi and Clostridium botulinum types C and D are of current interest because of their association with serious infections in injecting drug users (C. novyi type A) and equine and feline dysautonomias (C. botulinum types C/D). The species are defined by the major toxins they produce: th...

journal_title：Journal of medical microbiology

authors： Heffron A,Poxton IR

更新日期：2007-02-01 00:00:00

abstract：:Two monoclonal antibodies (MAbs) to a 36-kDa extracellular metalloprotease (PSCP) from Burkholderia (Pseudomonas) cepacia were found to react with thermolysin, Pseudomonas aeruginosa elastase, alkaline protease (Apr) and LasA, Serratia marcescens protease (SMP), Aeromonas hydrophila protease (AhP), and both the lethal...

journal_title：Journal of medical microbiology

authors： Kooi C,Sokol PA

更新日期：1996-09-01 00:00:00

abstract：:Corynebacterium jeikeium, a member of the non-diphtheria corynebacteria, has been rarely reported as being responsible for cardiovascular-device infection. Here, we report what is believed to be the first case of C. jeikeium pacemaker infection associated with the presence of proteinase-3 antineutrophil cytoplasmic an...

journal_title：Journal of medical microbiology

authors： Bechara C,Gousseff M,Passeron A,Podglajen I,Day N,Pouchot J,Lavergne T,Mainardi JL

更新日期：2011-02-01 00:00:00

abstract：:Purpose. Antimicrobial susceptibility is slow to determine, taking several days to fully impact treatment. This proof-of-concept study assessed the feasibility of using machine-learning techniques for analysis of data produced by the flow cytometer-assisted antimicrobial susceptibility test (FAST) method we developed....

journal_title：Journal of medical microbiology

authors： Inglis TJJ,Paton TF,Kopczyk MK,Mulroney KT,Carson CF

更新日期：2020-05-01 00:00:00

abstract：:Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospiros...

journal_title：Journal of medical microbiology

authors： Brown PD,Gravekamp C,Carrington DG,van de Kemp H,Hartskeerl RA,Edwards CN,Everard CO,Terpstra WJ,Levett PN

更新日期：1995-08-01 00:00:00