Characterization of the charge variants of L2 beta-lactamase in Stenotrophomonas maltophilia.

Abstract:

:Stenotrophomonas maltophilia KH has two acid beta-lactamases with isoelectric points (pIs) of 4.6 and 5.4, and several basic beta-lactamases (pIs >7.0) that produce a ladder-shaped pattern by IEF. An isogenic L2 mutant, KHL2(xylE), was constructed by gene replacement. From IEF and native PAGE zymograms of strains KH and KHL2(xylE), it was demonstrated that the basic beta-lactamases and the acid beta-lactamase with pI 5.4 are encoded by the same L2 gene and that the active types of these L2 charge variants were dependent on the buffer pH. The beta-lactamase activities of these L2 charge variants in phosphate buffer at pH 7.0 and 8.0 were 1075+/-29 and 1114+/-81 U mg(-1), respectively. These results indicate that L2 charge variants give S. maltophilia a better chance of adapting and surviving in response to changes in the environment.

journal_name

J Med Microbiol

authors

Hu RM,Chiang KH,Chang YC,Yang TC

doi

10.1099/jmm.0.000380-0

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

318-321

issue

Pt 3

eissn

0022-2615

issn

1473-5644

journal_volume

58

pub_type

杂志文章
  • Nosocomial infection with Asaia lannensis in two paediatric patients with idiopathic dilated cardiomyopathy.

    abstract::This is the first report, to our knowledge, of two temporally and geographically related nosocomial Asaia lannensis infections in a paediatric setting. Two patients with idiopathic dilated cardiomyopathy awaiting cardiac transplantation developed bacteraemia during their hospital stay. The physical location of both pa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019067-0

    authors: Juretschko S,Beavers-May TK,Stovall SH

    更新日期:2010-07-01 00:00:00

  • Role of quorum sensing in the pathogenicity of Burkholderia pseudomallei.

    abstract::Burkholderia pseudomallei is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the in vivo pathogenicity of B. pseudomallei via inhalational exposure of BALB/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that B. pseu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45661-0

    authors: Ulrich RL,DeShazer D,Brueggemann EE,Hines HB,Oyston PC,Jeddeloh JA

    更新日期:2004-11-01 00:00:00

  • Airborne transmission of lyssaviruses.

    abstract::In 2002, a Scottish bat conservationist developed a rabies-like disease and subsequently died. This was caused by infection with European bat lyssavirus 2 (EBLV-2), a virus closely related to Rabies virus (RABV). The source of this infection and the means of transmission have not yet been confirmed. In this study, the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46370-0

    authors: Johnson N,Phillpotts R,Fooks AR

    更新日期:2006-06-01 00:00:00

  • Spread of the Brazilian epidemic clone of a multiresistant MRSA in two cities in Argentina.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is recognised as an important cause of nosocomial infection. The spread of some MRSA epidemic clones is well documented. In Brazil, and more recently in Portugal, a considerable number of hospital infections has been caused by a unique multiresistant MRSA clone design...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-2-187

    authors: Coimbra MVDS,Teixeira LA,Ramos RLB,Predari SC,Castello L,Famiglietti A,Vay C,Klan L,Figueiredo AMS

    更新日期:2000-02-01 00:00:00

  • The role of breast-feeding in the prevention of rotavirus infection.

    abstract::Breast-fed infants are less susceptible to gastroenteritis than bottle-fed infants. Antibodies against rotavirus, the major pathogen of infantile gastroenteritis, were sought in human sera, colostrum and milk specimens by immunofluorescence. An experimental murine-rotavirus model was established by infecting the secon...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-1-25

    authors: Schoub BD,Prozesky OW,Lecatsas G,Oosthuizen R

    更新日期:1978-02-01 00:00:00

  • Characterization of the clonal subpopulation Fiocruz L1-130 of Leptospira interrogans in rats and dogs from Brazil.

    abstract:PURPOSE:Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in their hosts, and zoonotic outbreaks have been described. The genetic si...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000806

    authors: Jaeger LH,Pestana CP,Carvalho-Costa FA,Medeiros MA,Lilenbaum W

    更新日期:2018-09-01 00:00:00

  • Phenotypic and molecular characterization of antimicrobial resistance in Proteus mirabilis isolates from dogs.

    abstract::Large-scale monitoring of resistance to 14 antimicrobial agents was performed using 103 Proteus mirabilis strains isolated from dogs in Japan. Resistant strains were analysed to identify their resistance mechanisms. Rates of resistance to chloramphenicol, streptomycin, enrofloxacin, trimethoprim/sulfamethoxazole, kana...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.081539-0

    authors: Harada K,Niina A,Shimizu T,Mukai Y,Kuwajima K,Miyamoto T,Kataoka Y

    更新日期:2014-11-01 00:00:00

  • Voriconazole susceptibility of yeasts isolated from the mouths of patients with advanced cancer.

    abstract::The in vitro activity of voriconazole was compared with those of fluconazole and itraconazole against 270 clinical isolates of yeasts from the mouths of patients receiving palliative care for advanced cancer. A broth micro-dilution assay as described by the National Committee for Clinical Laboratory Standards was empl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45720-0

    authors: Bagg J,Sweeney MP,Davies AN,Jackson MS,Brailsford S

    更新日期:2005-10-01 00:00:00

  • Plasmid content and localisation of the STaI (STaP) gene in enterotoxigenic Escherichia coli with a non-radioactive polynucleotide gene probe.

    abstract::Enterotoxigenic Escherichia coli (ETEC) strain P2200 of porcine origin possessed eight possibly plasmid-determined characters (K88+ Raf+ Hly+ Col+ Smr Tcr Su(r) STa+) and six plasmid DNA bands of 4.2-93 kb. Analysis of the spontaneous loss of characters and the results of matings with other E. coli strains revealed th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-37-2-141

    authors: Bertin A

    更新日期:1992-08-01 00:00:00

  • Immunogenicity of mycobacterial PPE44 (Rv2770c) in Mycobacterium bovis BCG-infected mice.

    abstract::The Pro-Pro-Glu (PPE) protein family of Mycobacterium tuberculosis includes 69 glycine-rich proteins with a conserved N-terminal domain. Their role in tuberculosis is unknown, but it has been speculated that they may have an important immunological significance. In this investigation, the immunogenicity of the ppe44 (...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45960-0

    authors: Bonanni D,Rindi L,Lari N,Garzelli C

    更新日期:2005-05-01 00:00:00

  • Assessment of the in vitro and in vivo activity of atorvastatin against Candida albicans.

    abstract::Aim. The aim of this work was to characterize the response of Candida albicans to atorvastatin, and to assess its in vivo antifungal capability.Methodology. The effect of atorvastatin on the growth and viability of C. albicans was assessed. The ability of the statin to alter cell permeability was quantified by measuri...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001065

    authors: Ajdidi A,Sheehan G,Abu Elteen K,Kavanagh K

    更新日期:2019-10-01 00:00:00

  • Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates.

    abstract::The susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016444-0

    authors: Raponi G,Ghezzi MC,Gherardi G,Lorino G,Dicuonzo G

    更新日期:2010-06-01 00:00:00

  • A study of the evolution of specific and non-specific immune complexes in acute hepatitis B and chronic hepatitis.

    abstract::Circulating immune complexes (ICs) containing IgG and HBsAg, and IgG and HBeAg, in sera from groups of patients with various liver diseases were sought by ELISA and immunodiffusion. A correlation was found between the absence of ICs and the disappearance of HBsAg in patients who had recovered from acute hepatitis B, b...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-3-237

    authors: Leyva A,Bernal MC,Piedrola G,Maroto MC

    更新日期:1988-07-01 00:00:00

  • A mixed infection of Leuconostoc lactis and vancomycin-resistant Enterococcus in a liver transplant recipient.

    abstract::Bacterial infection in patients who have undergone liver transplantation is a major complication of the procedure. Leuconostoc spp. are important pathogenic bacteria in individuals with poor immune function, especially transplant patients. In this report, we describe the case of a 45-year-old Asian male liver transpla...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.045161-0

    authors: Deng Y,Zhang Z,Xie Y,Xiao Y,Kang M,Fan H

    更新日期:2012-11-01 00:00:00

  • Comparison of conventional culture with SeptiFast real-time PCR for microbial pathogen detection in clinical specimens other than blood.

    abstract::Early detection of aetiological agents is pivotal for adequate therapy for bacterial infections. Although culture is still considered the mainstay for laboratory diagnosis, it often lacks sensitivity, especially in patients already treated with antibiotics. The present study investigated the potential clinical utility...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034280-0

    authors: Mencacci A,Leli C,Cardaccia A,Montagna P,Moretti A,Bietolini C,Meucci M,Perito S,Cenci E,Bistoni F

    更新日期:2011-12-01 00:00:00

  • Enzyme-linked immunosorbent assay for brucella antigen detection in human sera.

    abstract::A sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody coupled to the solid phase was evaluated for the detection of brucella antigen in serum samples. Under optimum conditions, 100 brucella cells/well or 10(5) fg/ml of lipopolysaccharide (LPS) were detected in spiked specimens. The standardised...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-2-169

    authors: Al-Shamahy HA,Wright SG

    更新日期:1998-02-01 00:00:00

  • Yokenella regensburgei infection in India mimicking enteric fever.

    abstract::Yokenella regensburgei is an opportunistic human pathogen of the Enterobacteriaceae family rarely reported to cause human infections. Here, we present a case report of Y. regensburgei bacteraemia from India clinically resembling enteric fever in an apparently immunocompetent paediatric patient. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.058669-0

    authors: Jain S,Gaind R,Gupta KB,Dawar R,Kumar D,Paul P,Sardana R,Deb M

    更新日期:2013-06-01 00:00:00

  • Evaluation of antigenic variations between two virulent toxoplasma strains.

    abstract::Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies o...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46712-0

    authors: Delibas SB,Ertabaklar H,Ertug S

    更新日期:2006-10-01 00:00:00

  • Characteristics of cysteine-requiring strains of Klebsiella isolated from urinary tract infections.

    abstract::Clinical and bacteriological findings in seven cases of urinary tract infection with cysteine-requiring strains of Klebsiella are described. The organisms were isolated from patients with long-standing urinary tract abnormalities and grew as small (c. 1 mm) colonies on MacConkey agar. The organisms failed to grow in a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-3-211

    authors: McIver CJ,Tapsall JW

    更新日期:1988-07-01 00:00:00

  • Successful substitution of fetal calf serum by human plasma for bulk cultivation of Leishmania donovani promastigotes.

    abstract::The potential of human plasma (HP) or serum (HS) as a replacement for fetal calf serum (FCS) was evaluated in a liver infusion tryptose (LIT) medium for bulk cultivation of Leishmania donovani promastigotes. The promastigote yield with the LIT-FCS standard medium was 0.4-1.8×10(7) ml(-1), and yields of 0.5-3.4×10(7) (...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.052993-0

    authors: Mahamoud A,Osman HA,Mansour D,El Harith A

    更新日期:2013-08-01 00:00:00

  • Prospective evaluation of BDProbeTec strand displacement amplification (SDA) system for diagnosis of tuberculosis in non-respiratory and respiratory samples.

    abstract::Nucleic acid amplification techniques (NAATs) have been demonstrated to make significant improvements in the diagnosis of tuberculosis (TB), particularly in the time to diagnosis and the diagnosis of smear-negative TB. The BD ProbeTec strand displacement amplification (SDA) system for the diagnosis of pulmonary and no...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45780-0

    authors: McHugh TD,Pope CF,Ling CL,Patel S,Billington OJ,Gosling RD,Lipman MC,Gillespie SH

    更新日期:2004-12-01 00:00:00

  • Gas-lipuid chromatographic analysis of metabolic products in the identification of bacteroidaceae of clinical interest.

    abstract::The acid end-products of 185 isolates from the family Bacteroidaceae were separated and analysed by gas-liquid chromatography on broth cultures. Different media were evaluated and definitive studies were performed in a fully supplemented complex medium. The limitations of this approach to the identification of a wide ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-2-81

    authors: Deacon AG,Duerden BI,Holbrook WP

    更新日期:1978-05-01 00:00:00

  • Isolation of Carnobacterium sp. from a human blood culture.

    abstract::Carnobacterium species have been isolated from the environment and are not regarded as human pathogens, although they are known to cause disease in fish. Only two reports describing isolation of Carnobacterium species from human pus were found in the literature. We report what we believe to be the first isolation of C...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016808-0

    authors: Hoenigl M,Grisold AJ,Valentin T,Leitner E,Zarfel G,Renner H,Krause R

    更新日期:2010-04-01 00:00:00

  • Antibiotic-induced release of endotoxin from bacteria in vitro.

    abstract::The ability of cefotaxime, ciprofloxacin, piperacillin and tobramycin to cause release of endotoxin was examined in vitro with cultures of Enterobacter cloacae and Escherichia coli. Endotoxin was measured by a quantitative limulus amoebocyte lysate assay and its presence was confirmed by silver staining of the lipopol...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-1-23

    authors: Crosby HA,Bion JF,Penn CW,Elliott TS

    更新日期:1994-01-01 00:00:00

  • Laboratory challenges in the diagnosis of hepatitis E virus.

    abstract::Hepatitis E virus (HEV) is an RNA virus that is an important cause of both acute and chronic hepatitis worldwide. To date, there are eight HEV genotypes that can infect mammals. HEV-1 and HEV-2 infect exclusively humans, while HEV-3 and HEV-4 infect humans and various animals, mainly pigs and deer. Additionally, two n...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.000706

    authors: Al-Sadeq DW,Majdalawieh AF,Mesleh AG,Abdalla OM,Nasrallah GK

    更新日期:2018-04-01 00:00:00

  • Severe case of Mediterranean spotted fever in Greece with predominantly neurological features.

    abstract::Rickettsia conorii is endemic in Greece, though only a few cases of infection have been published to date. The case of a 58-year-old man from northern Greece with a severe form of Mediterranean spotted fever and rapid neurological deterioration is presented here. The patient received antibiotic treatment with doxycycl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46337-0

    authors: Tzavella K,Hatzizisis IS,Vakali A,Mandraveli K,Zioutas D,Alexiou-Daniel S

    更新日期:2006-03-01 00:00:00

  • Bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization.

    abstract::Oral biofilms are one of the greatest challenges in dental research. The present study aimed to investigate initial bacterial colonization of enamel surfaces in situ using fluorescence in situ hybridization (FISH) over a 12 h period. For this purpose, bovine enamel slabs were fixed on buccal sites of individual splint...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.011213-0

    authors: Al-Ahmad A,Follo M,Selzer AC,Hellwig E,Hannig M,Hannig C

    更新日期:2009-10-01 00:00:00

  • Studies on the Vibrio cholerae mucinase complex. III. Neutralisation of the neuraminidase activity by specific anti-neuraminidase IgG.

    abstract::A partially-purified neuraminidase from the mucinase complex of Vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. When the neutralising potency of this serum against V. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-25-2-123

    authors: Stewart-Tull DE,Ollar RA

    更新日期:1988-02-01 00:00:00

  • Identification of catalase-like activity from Mycobacterium leprae and the relationship between catalase and isonicotinic acid hydrazide (INH).

    abstract::As Mycobacterium leprae proliferate inside macrophages, it has been speculated that catalase encoded by katG may protect the bacilli from deleterious effects of peroxide generated from the macrophage and may also play a crucial role in the survival of M. leprae in vivo. However, unlike that of M. tuberculosis, the kat...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-8-675

    authors: Kang TJ,You JC,Chae GT

    更新日期:2001-08-01 00:00:00

  • Graft versus host disease-related Hafnia alvei colonization and probable infection.

    abstract::We describe the case of a graft versus host disease (GvHD) patient, in whom Hafnia alvei was cultured as a single organism, and at high bacterial counts from stool samples, from the onset of the disease until its resolution. This case is a further example of the contentious role of this species in causing human intest...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.2008/001164-0

    authors: Savini V,Di Bartolomeo E,Catavitello C,Talia M,Manna A,Febbo F,Balbinot A,Di Bonaventura G,Di Bartolomeo P,Piccolomini R,D'Antonio D

    更新日期:2008-09-01 00:00:00