Enzyme-linked immunosorbent assay for brucella antigen detection in human sera.

abstract：:Purpose. Antimicrobial susceptibility is slow to determine, taking several days to fully impact treatment. This proof-of-concept study assessed the feasibility of using machine-learning techniques for analysis of data produced by the flow cytometer-assisted antimicrobial susceptibility test (FAST) method we developed....

journal_title：Journal of medical microbiology

authors： Inglis TJJ,Paton TF,Kopczyk MK,Mulroney KT,Carson CF

更新日期：2020-05-01 00:00:00

abstract：:The family Adenoviridae consists of five genera of which the genus Mastadenovirus includes human viruses classified into 57 serotypes clustered into seven subgroups (A-G). Serotypes 40 and 41 (subgroup F) are specifically associated with childhood gastroenteritis and are the most common cause of acute gastroenteritis ...

journal_title：Journal of medical microbiology

authors： Arcangeletti MC,Germini D,Martorana D,Rodighiero I,De Conto F,Medici MC,Chezzi C,Calderaro A

更新日期：2014-06-01 00:00:00

abstract：:The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis....

journal_title：Journal of medical microbiology

authors： Ikeda-Dantsuji Y,Konomi I,Nagayama A

更新日期：2005-04-01 00:00:00

abstract：:Catechin, a constituent of tea, possesses various bioactivities. In particular, the most abundant catechin in tea is epigallocatechin gallate (EGCg), which has an anti-inflammatory effect. In the present study, the usability of EGCg for osteomyelitis treatment was examined. Osteomyelitis is a difficult disease to cure...

journal_title：Journal of medical microbiology

authors： Ishida I,Kohda C,Yanagawa Y,Miyaoka H,Shimamura T

更新日期：2007-08-01 00:00:00

abstract：:Circulating immune complexes (ICs) containing IgG and HBsAg, and IgG and HBeAg, in sera from groups of patients with various liver diseases were sought by ELISA and immunodiffusion. A correlation was found between the absence of ICs and the disappearance of HBsAg in patients who had recovered from acute hepatitis B, b...

journal_title：Journal of medical microbiology

authors： Leyva A,Bernal MC,Piedrola G,Maroto MC

更新日期：1988-07-01 00:00:00

abstract：:The prevalence of Shiga toxin-producing Escherichia coli (STEC) and its characteristics were determined among hospitalized patients with diarrhoea and children with diarrhoea in an urban slum community of Dhaka city using sensitive culture and PCR methods. Stool samples were collected from 410 patients with diarrhoea ...

journal_title：Journal of medical microbiology

authors： Islam MA,Heuvelink AE,de Boer E,Sturm PD,Beumer RR,Zwietering MH,Faruque ASG,Haque R,Sack DA,Talukder KA

更新日期：2007-03-01 00:00:00

abstract：:Gram-negative bacilli of the genus Aeromonas are widespread in aquatic environments and can be responsible for human infections. Although Aeromonas extraintestinal and systemic infections have been reported with growing frequency in recent years, Aeromonas septicaemia remains an uncommon finding, often associated with...

journal_title：Journal of medical microbiology

authors： Mencacci A,Cenci E,Mazzolla R,Farinelli S,D'Alò F,Vitali M,Bistoni F

更新日期：2003-08-01 00:00:00

abstract：:The effect of passive immunotherapy with antisera against heat-killed Pseudomonas aeruginosa and three of its exo-enzymes (elastase, alkaline protease and exotoxin A) in gut-derived P. aeruginosa sepsis was evaluated. Mice were given a suspension of P. aeruginosa strain D4 in their drinking water, together with ampici...

journal_title：Journal of medical microbiology

authors： Matsumoto T,Furuya N,Tateda K,Miyazaki S,Ohno A,Ishii Y,Hirakata Y,Yamaguchi K

更新日期：1999-08-01 00:00:00

abstract：:Acinetobacter baumannii is an emerging multidrug-resistant pathogen and very little information is available regarding its imipenem resistance in Latin American countries such as Bolivia. This study investigated the antimicrobial resistance profile of 46 clinical strains from different hospitals in Cochabamba, Bolivia...

journal_title：Journal of medical microbiology

authors： Sevillano E,Fernández E,Bustamante Z,Zabalaga S,Rosales I,Umaran A,Gallego L

更新日期：2012-01-01 00:00:00

abstract：:Motile curved rods seen in vaginal secretions have been isolated on Columbia agar supplemented with 5% human blood and vitamin K. Growth occurred anaerobically and in 5% oxygen but not in more aerobic conditions. There were two distinct groups of these organisms, distinguishable by morphology, biochemical activity and...

journal_title：Journal of medical microbiology

authors： Sprott MS,Ingham HR,Pattman RS,Eisenstadt RL,Short GR,Narang HK,Sisson PR,Selkon JB

更新日期：1983-05-01 00:00:00

abstract：:Enterotoxigenic Escherichia coli (ETEC) strain P2200 of porcine origin possessed eight possibly plasmid-determined characters (K88+ Raf+ Hly+ Col+ Smr Tcr Su(r) STa+) and six plasmid DNA bands of 4.2-93 kb. Analysis of the spontaneous loss of characters and the results of matings with other E. coli strains revealed th...

journal_title：Journal of medical microbiology

authors： Bertin A

更新日期：1992-08-01 00:00:00

abstract：:Francisella tularensis is a highly virulent intracellular bacterium capable of rapid multiplication in phagocytic cells. Previous studies have revealed that activation of F. tularensis-infected macrophages leads to control of infection and reactive nitrogen and oxygen species make important contributions to the bacter...

journal_title：Journal of medical microbiology

authors： Tancred L,Telepnev MV,Golovliov I,Andersson B,Andersson H,Lindgren H,Sjöstedt A

更新日期：2011-11-01 00:00:00

abstract：:The aim of this retrospective study was to determine the clinical spectrum of group B streptococcal (GBS) bacteraemia in patients over 70 years old. Sixty-six adults with GBS bacteraemia were reviewed over a 5-year period. Disease characteristics, clinical diagnoses and underlying disease were compared in 33 older pat...

journal_title：Journal of medical microbiology

authors： Trivalle C,Martin E,Martel P,Jacque B,Menard JF,Lemeland JF

更新日期：1998-07-01 00:00:00

abstract：:Shanson's medium, a MacConkey-type agar containing added inhibitors, was compared with deoxycholate-citrate agar for the examination of faeces. The isolation rate of samonellae on the two media was similar, but Shanson's medium was the more selective; this meant that the amount of work required to produce a result was...

journal_title：Journal of medical microbiology

authors： Davies JR

更新日期：1975-05-01 00:00:00

abstract：:Growth of Candida albicans biofilms and production of extracellular matrix were monitored by dry weight, colorimetric and radioisotope assays, and by scanning electron microscopy. Under static incubation conditions synthesis of matrix material was minimal, but increased dramatically when developing biofilms were subje...

journal_title：Journal of medical microbiology

authors： Hawser SP,Baillie GS,Douglas LJ

更新日期：1998-03-01 00:00:00

abstract：INTRODUCTION:Idiopathic pulmonary fibrosis (IPF) is a chronic and ultimately fatal lung disease. One of the risk factors involved in the acquisition of IPF is viral infections, especially respiratory viruses. In the present study, we investigated the detection of respiratory viruses and the possible relationship betwee...

journal_title：Journal of medical microbiology

authors： Keyvani H,Moghoofei M,Bokharaei-Salim F,Mostafaei S,Javad Mousavi SA,Monavari SH,Esghaei M

更新日期：2017-11-01 00:00:00

abstract：:A rabbit was immunised with a heat-killed vaccine prepared from strain 1142 of Staphylococcus epidermidis. The HA titres of the antisera against a surface-polysaccharide antigen extracted from the homologous strain were highest in the 2nd-4th weeks after immunisation and subsequently declined. Only a minor amount of 2...

journal_title：Journal of medical microbiology

authors： Yoshida K,Ichiman Y

更新日期：1978-11-01 00:00:00

abstract：:Conventional culture and drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are laborious and time consuming. For this reason alternative rapid culture and DST techniques are urgently needed to shorten the time for drug-resistance detection. A total of 222 smear-positive sputum samples were evalu...

journal_title：Journal of medical microbiology

authors： Imperiale BR,Morcillo NS,Palomino JC,Vandamme P,Martin A

更新日期：2014-04-01 00:00:00

abstract：:The agglutination of 218 clinical isolates and three ATCC type strains of "Streptococcus milleri" was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains...

journal_title：Journal of medical microbiology

authors： Kellens JT,Jacobs JA,Peumans WJ,Stobberingh EE

更新日期：1994-07-01 00:00:00

abstract：:Clostridium difficile-associated diarrhoea (CDAD) is the most common hospital-acquired diarrhoea, and is a major type of gastroenteritis infection in nursing homes and facilities for the elderly. In this study the antimicrobial activity of the two-component lantibiotic, lacticin 3,147, against a range of genetically d...

journal_title：Journal of medical microbiology

authors： Rea MC,Clayton E,O'Connor PM,Shanahan F,Kiely B,Ross RP,Hill C

更新日期：2007-07-01 00:00:00

abstract：:Campylobacter jejuni, when left on blood agar for prolonged periods, was found to survive better in air than under micro-aerobic conditions. After a period of 2-3 days in air, all strains of C. jejuni examined grew freely in air on subculture, and could be further subcultured apparently indefinitely in air. This adapt...

journal_title：Journal of medical microbiology

authors： Jones DM,Sutcliffe EM,Rios R,Fox AJ,Curry A

更新日期：1993-02-01 00:00:00

abstract：:Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without...

journal_title：Journal of medical microbiology

authors： Menon S,Stansfield SH,Logan B,Hocking JS,Timms P,Rombauts L,Allan JA,Huston WM

更新日期：2016-09-01 00:00:00

abstract：:Alpha protoxin of Staphylococcus aureus "Wood 46" was activated by trypsin which had been coupled to carboxymethylcellulose, as indicated by the toxin's ability to hydrolyse tosyl-arginine methylester (TAME). A Lineweaver-Burk plot of the degradation of TAME by toxin and trypsin showed that toxin had a greater affinit...

journal_title：Journal of medical microbiology

authors： Wiseman GM,Caird JD,Fackrell HB

更新日期：1975-02-01 00:00:00

abstract：:Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However, there are few published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD are essential to inform policy regarding pneumococcal conj...

journal_title：Journal of medical microbiology

authors： Jefferies JMC,Tee WSN,Clarke SC

更新日期：2011-06-01 00:00:00

abstract：:Sixty-three Staphylococcus aureus strains (40 from clinical sources and 23 from food sources) were examined for toxic shock syndrome toxin-1 (TSST-1) using PCR, phage typed using the international phage set (IPS) and tested for their susceptibility to antibiotics. Only three strains (all from clinical sources) were po...

journal_title：Journal of medical microbiology

authors： El-Ghodban A,Ghenghesh KS,Márialigeti K,Esahli H,Tawil A

更新日期：2006-02-01 00:00:00

abstract：:A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated...

journal_title：Journal of medical microbiology

authors： Pourshafie MR,Grimont F,Saifi M,Grimont PAD

更新日期：2000-12-01 00:00:00

abstract：:Mycoplasma fermentans has attracted much interest both as a cofactor for the progression of AIDS and as a pathogenic agent in non-AIDS related diseases. Previous studies with serological and genetic techniques suggest that M. fermentans represents a homogeneous group of organisms, with no significant differences ident...

journal_title：Journal of medical microbiology

authors： Hannan PC,Kearns AM,Sisson PR,Freeman R

更新日期：1997-04-01 00:00:00

abstract：:The ability of various haem- and non-haem-iron-containing compounds to support the growth of iron-limited cultures of Haemophilus ducreyi was assessed in a plate bioassay. Only haemin or the haem-containing proteins, bovine haemoglobin, human haemoglobin and bovine catalase, but not equine cytochrome C111, were capabl...

journal_title：Journal of medical microbiology

authors： Lee BC

更新日期：1991-06-01 00:00:00

abstract：:The surface structures of the cell envelopes of 16 clinical isolates of Pseudomonas aeruginosa were examined by electronmicroscopy with the new fixation technique of freeze-substitution. Two types of structures were observed among the organisms. In one group of strains, mostly isolated from blood, a dense fibrous laye...

journal_title：Journal of medical microbiology

authors： Misumi H,Umeda A,Eguchi K,Okada K,Sawae Y,Niho Y,Amako K

更新日期：1994-04-01 00:00:00

abstract：:Rhodococcus equi is one of the most important causes of pneumonia in foals and has emerged as a significant opportunistic pathogen of immunosuppressed hosts such as human immunodeficiency virus-infected patients. Virulent R. equi harbouring an 85 kb plasmid, but not the avirulent form lacking the plasmid, has the abil...

journal_title：Journal of medical microbiology

authors： Toyooka K,Takai S,Kirikae T

更新日期：2005-11-01 00:00:00