Quantitative expression of cholera toxin mRNA in Vibrio cholerae isolates with different CTX cassette arrangements.

abstract：PURPOSE:Platelet concentrates are at risk of transfusion-related sepsis. The microbial detection methods currently available have reached their limits, as they do not completely prevent transfusion-related bacterial contamination.The aim of this study was to develop a new strategy to detect the risk of platelet transfu...

journal_title：Journal of medical microbiology

authors： Chetouane Y,Dubourg G,Gallian P,Delerce J,Levasseur A,Flaudrops C,Chabrière E,Chiaroni J,Raoult D,Camoin-Jau L

更新日期：2017-11-01 00:00:00

abstract：:The therapeutic alternatives available for use against ciprofloxacin-resistant enteric fever isolates in an endemic area are limited. The antibiotics currently available are the quinolones, third-generation cephalosporins and conventional first-line drugs. In this study, the MICs of various newer drugs were determined...

journal_title：Journal of medical microbiology

authors： Capoor MR,Rawat D,Nair D,Hasan AS,Deb M,Aggarwal P,Pillai P

更新日期：2007-11-01 00:00:00

abstract：:Pseudomonas aeruginosa produces multiple virulence factors and causes different types of infections. Previous clinical studies identified P. aeruginosa isolates that lack individual virulence factors. However, the impact of losing several virulence factors simultaneously on the in vivo virulence of P. aeruginosa is no...

journal_title：Journal of medical microbiology

authors： Schaber JA,Carty NL,McDonald NA,Graham ED,Cheluvappa R,Griswold JA,Hamood AN

更新日期：2004-09-01 00:00:00

abstract：PURPOSE:Nodal may play an important role in the development of cancers. The present study was designed to determine the effects of Nodal induced by respiratory syncytial virus (RSV) infection on the occurrence and development of lung cancer and the underlying mechanisms. METHODOLOGY:After verification of RSV infection...

journal_title：Journal of medical microbiology

authors： Xiang Z,Liang Z,Yanfeng H,Leitao K

更新日期：2017-10-01 00:00:00

abstract：:The resistance of Balamuthia mandrillaris to physical, chemical and radiological conditions was tested. Following treatments, viability was determined by culturing amoebae on human brain microvascular endothelial cells for up to 12 days. B. mandrillaris cysts were resistant to repeated freeze-thawing (five times), tem...

journal_title：Journal of medical microbiology

authors： Siddiqui R,Ortega-Rivas A,Khan NA

更新日期：2008-04-01 00:00:00

abstract：:Fifty clinical strains of Chlamydia trachomatis were studied by denaturing gradient gel electrophoresis (DGGE) of bacterial DNA amplified by the polymerase chain reaction (PCR). The strains belonged to the three most commonly encountered serovars in developed countries--D, E and F. Six reference strains, including the...

journal_title：Journal of medical microbiology

authors： Sayada C,Denamur E,Grandchamp B,Orfila J,Elion J

更新日期：1995-07-01 00:00:00

abstract：:Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...

journal_title：Journal of medical microbiology

authors： Tran HD,Alam M,Trung NV,Van Kinh N,Nguyen HH,Pham VC,Ansaruzzaman M,Rashed SM,Bhuiyan NA,Dao TT,Endtz HP,Wertheim HFL

更新日期：2012-03-01 00:00:00

abstract：:Rhodococcus equi is an opportunistic human pathogen associated with immunosuppressed people. While the interaction of R. equi with macrophages has been comprehensively studied, little is known about its interactions with non-phagocytic cells. Here, we characterized the entry process of this bacterium into human lung e...

journal_title：Journal of medical microbiology

authors： Remuzgo-Martínez S,Pilares-Ortega L,Álvarez-Rodríguez L,Aranzamendi-Zaldunbide M,Padilla D,Icardo JM,Ramos-Vivas J

更新日期：2013-08-01 00:00:00

abstract：:Introduction. Pseudomonas aeruginosa is an important Gram-negative pathogen that is intrinsically multidrug-resistant (MDR) and frequently associated with healthcare-associated outbreaks. With increasing resistance to antibiotics and with very few novel drugs under development, clinicians often use combinations to tre...

journal_title：Journal of medical microbiology

authors： Betts JW,Hornsey M,Higgins PG,Lucassen K,Wille J,Salguero FJ,Seifert H,La Ragione RM

更新日期：2019-10-01 00:00:00

abstract：:Amplification and sequence analysis of the 16S rRNA gene can be applied to detect and identify bacteria in clinical samples. We examined 75 clinical samples (17 culture-positive, 58 culture-negative) prospectively by two different PCR protocols, amplifying either a single fragment (1343 bp) or two fragments (762/598 b...

journal_title：Journal of medical microbiology

authors： Jenkins C,Ling CL,Ciesielczuk HL,Lockwood J,Hopkins S,McHugh TD,Gillespie SH,Kibbler CC

更新日期：2012-04-01 00:00:00

abstract：:Bacterially-driven mucosal inflammation and the development of periimplantitis can lead to oral implant failure. In this study, initial bacterial adhesion after 2 h, and biofilm formation after 1 day and 3 days, were analysed in situ on novel 3 mol % yttria-stabilized tetragonal zirconia polycrystal samples, as well a...

journal_title：Journal of medical microbiology

authors： Al-Ahmad A,Karygianni L,Schulze Wartenhorst M,Bächle M,Hellwig E,Follo M,Vach K,Han JS

更新日期：2016-07-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:Epidemic methicillin-resistant Staphylococcus aureus types 15 and 16 (EMRSA-15 and EMRSA-16) are the dominant types of MRSA found in UK hospitals, but accurate designation of strains has been difficult. Restriction fragment length polymorphism (RFLP) profiles of seven core virulence genes were used to classify unambig...

journal_title：Journal of medical microbiology

authors： Moore PCL,Lindsay JA

更新日期：2002-06-01 00:00:00

abstract：:The lipopolysaccharides (LPS) of the 10 species of the genus Bacteroides (sensu stricto) were extracted by the proteinase K method and their resolution compared by several methods of polyacrylamide gel electrophoresis (PAGE). These included sodium dodecyl sulphate (SDS)-PAGE with and without urea, polyacrylamide gradi...

journal_title：Journal of medical microbiology

authors： Maskell JP

更新日期：1991-05-01 00:00:00

abstract：:Sixty-three Staphylococcus aureus strains (40 from clinical sources and 23 from food sources) were examined for toxic shock syndrome toxin-1 (TSST-1) using PCR, phage typed using the international phage set (IPS) and tested for their susceptibility to antibiotics. Only three strains (all from clinical sources) were po...

journal_title：Journal of medical microbiology

authors： El-Ghodban A,Ghenghesh KS,Márialigeti K,Esahli H,Tawil A

更新日期：2006-02-01 00:00:00

abstract：:In a prospective study between February 2003 and June 2004, stool specimens of children less than 2 years of age with diarrhoea (n=218) and without diarrhoea (n=86), living in Vitória, Espírito Santo, Brazil, were examined for the presence of diarrhoeagenic Escherichia coli. E. coli isolates were tested by colony blot...

journal_title：Journal of medical microbiology

authors： Spano LC,Sadovsky ADI,Segui PN,Saick KW,Kitagawa SMS,Pereira FEL,Fagundes-Neto U,Scaletsky ICA

更新日期：2008-03-01 00:00:00

abstract：:Escherichia coli (2), Vibrio cholerae (2) and Aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. Three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. Clear differences wer...

journal_title：Journal of medical microbiology

authors： Ala Aldeen DA,Barer MR

更新日期：1989-04-01 00:00:00

abstract：:Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but not for the multiplication of Giardia duodenalis in the intestinal lumen. The microbial components responsible for this phenomenon are not known. Twenty-eight facultative and three strictly anaerobic micro-organisms were is...

journal_title：Journal of medical microbiology

authors： Torres MF,Uetanabaro APT,Costa AF,Alves CA,Farias LM,Bambirra EA,Penna FJ,Vieira EC,Nicoli JR

更新日期：2000-03-01 00:00:00

abstract：:Anaplasma phagocytophilum, an agent of human granulocytic anaplasmosis, infects neutrophils and causes an emerging tickborne febrile disease. The genome of this bacterium contains a large number of p44/msp2-related genes encoding 44 kDa major outer-membrane proteins, and it is known that a specific p44/msp2 gene is pr...

journal_title：Journal of medical microbiology

authors： Wuritu,Ozawa Y,Gaowa,Kawamori F,Masuda T,Masuzawa T,Fujita H,Ohashi N

更新日期：2009-12-01 00:00:00

abstract：:The family Adenoviridae consists of five genera of which the genus Mastadenovirus includes human viruses classified into 57 serotypes clustered into seven subgroups (A-G). Serotypes 40 and 41 (subgroup F) are specifically associated with childhood gastroenteritis and are the most common cause of acute gastroenteritis ...

journal_title：Journal of medical microbiology

authors： Arcangeletti MC,Germini D,Martorana D,Rodighiero I,De Conto F,Medici MC,Chezzi C,Calderaro A

更新日期：2014-06-01 00:00:00

abstract：PURPOSE:Extracellular protein toxins contribute to the pathogenesis of Staphylococcus aureus infections. The present study compared the effects of iclaprim and trimethoprim - two folic acid synthesis inhibitors - with nafcillin and vancomycin on production of Panton-Valentine leukocidin (PVL), alpha haemolysin (AH) and...

journal_title：Journal of medical microbiology

authors： Bryant AE,Gomi S,Katahira E,Huang DB,Stevens DL

更新日期：2019-03-01 00:00:00

abstract：:A total of 36 Escherichia coli and 31 enterococci isolates were recovered from 42 common buzzard faecal samples. The E. coli isolates showed high levels of resistance to streptomycin and tetracycline. The following resistance genes were detected: bla(TEM) (20 of 22 ampicillin-resistant isolates), tet(A) and/or tet(B) ...

journal_title：Journal of medical microbiology

authors： Radhouani H,Poeta P,Gonçalves A,Pacheco R,Sargo R,Igrejas G

更新日期：2012-06-01 00:00:00

abstract：:The association of Aeromonas spp. with travellers' diarrhoea was studied among 978 Finnish tourists travelling to Morocco in winter (n = 398) and autumn (n = 580) in 1989. Fifty-five isolates from diarrhoeal patients with (n = 16) or without (n = 39) a recent travelling history in a developing country were also includ...

journal_title：Journal of medical microbiology

authors： Hänninen ML,Salmi S,Mattila L,Taipalinen R,Siitonen A

更新日期：1995-01-01 00:00:00

abstract：:To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...

journal_title：Journal of medical microbiology

authors： Sugawara I,Yamada H,Kazumi Y,Doi N,Otomo K,Aoki T,Mizuno S,Udagawa T,Tagawa Y,Iwakura Y

更新日期：1998-10-01 00:00:00

abstract：:Encapsulation of purified tuberculoproteins in liposomes augmented their ability to elicit delayed hypersensitivity reactions in BCG-immune rats. The effect was most marked with a low-molecular-weight tuberculopeptide that was relatively poor at eliciting reactions when in free form. These findings indicate that, in a...

journal_title：Journal of medical microbiology

authors： Pimm MV,Baldwin RW

更新日期：1984-12-01 00:00:00

abstract：:We studied the immunoglobulin response to individual viral polypeptides in experimental primary and secondary infection with herpes simplex virus (HSV)-1 in mice. After a single footpad inoculation with 10(5.6) pfu of HSV-1, immunoglobulin to proteins mol. wts (10(3)) 44 and 75 appeared on day 7. Antibodies to gB, gC,...

journal_title：Journal of medical microbiology

authors： McKendall RR,Pettit M,Woo W

更新日期：1988-01-01 00:00:00

abstract：:The ability of rabbits to clear an intraperitoneal injection of Pseudomonas aeruginosa in the presence or absence of a surgically implanted peritoneal device was investigated. Sham-operated rabbits without an implant eliminated a P. aeruginosa challenge of 5 x 10(6) cfu/ml; lavage fluid and peritoneal tissues became c...

journal_title：Journal of medical microbiology

authors： Ward KH,Olson ME,Lam K,Costerton JW

更新日期：1992-06-01 00:00:00

abstract：:During the first quarter of 1996, a major outbreak of clinical infection caused by multiresistant Klebsiella pneumoniae (MRKP) occurred in the neonatal ward of the 'Maternité Wassila Bourguiba' in Tunis, Tunisia. In total, 32 isolates of MRKP, comprising 23 clinical isolates and nine surveillance isolates, were recove...

journal_title：Journal of medical microbiology

authors： Ben-Hamouda T,Foulon T,Ben-Cheikh-Masmoudi A,Fendri C,Belhadj O,Ben-Mahrez K

更新日期：2003-05-01 00:00:00

abstract：:Tests with 140 strains representing Escherichia coli, Klebsiella aerogenes, K. ozaenae, Proteus mirabilis, P. vulgaris, P. rettgeri and P. morganii in a defined medium supplemented with 0-09M dimethylamine (DMA) and 0-1M potassium nitrate showed that at least 89% of the 136 strains able to reduce nitrates produced up ...

journal_title：Journal of medical microbiology

authors： Coloe PJ,Hayward NJ

更新日期：1976-05-01 00:00:00

abstract：:Campylobacter strains isolated from the same stool sample were characterised by determination of biochemical properties and both heat-labile (Lior) and heat-stable (Lauwers) serotypes. In six of 60 campylobacter-infected stools, two or three strains differing in Lior-serotype were isolated from the same stool. In four...

journal_title：Journal of medical microbiology

authors： Zöllner B,Wuthe HH

更新日期：1993-01-01 00:00:00