Induction of mutation in Staphylococcus aureus by ethylmethane sulphonate.

Abstract:

:Staphylococcus aureus strains were treated with ethylmethane sulphonate with the object of obtaining mutants that differed from the wild type in one of the properties that might contribute to virulence. Mutants with isolated loss or deficient production of gamma-haemolysin, coagulase, leucocidin and staphylokinase were obtained. Changes in more than one property were often observed. The loss of delta-lysin production was always coupled with an appreciable reduction in P-V leucocidin production. With that exception, systematised combined loss of a restricted number of staphylococcal characteristics was not observed. Mutants that had lost all characteristics of S. aureus were phage-resistant. Isolated loss of staphylokinase production and deficient P-V leucocidin production were found to be due to the independent loss of two prophages.

journal_name

J Med Microbiol

authors

van der Vijver JC,van Es-Boon MM,Michel MF

doi

10.1099/00222615-8-2-265

subject

Has Abstract

pub_date

1975-05-01 00:00:00

pages

265-77

issue

2

eissn

0022-2615

issn

1473-5644

journal_volume

8

pub_type

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