Genetic modification of a vaginal strain of Lactobacillus fermentum and its maintenance within the reproductive tract after intravaginal administration.

Abstract:

:Many micro-organisms cause important diseases of the female genital tract. Because systematic vaccination does not usually provide a good immune response at mucosal sites, commensal lactobacilli from the female genital tract were developed as vehicles to deliver continued doses of foreign antigen directly to the genital mucosal surface with the aim of stimulating strong local mucosal immune responses. Lactobacilli were shown to be common inhabitants of the genital tract of the animal model studied, the guinea-pig. One species, Lactobacillus fermentum, was found in all guinea-pigs studied and was chosen for genetic manipulation. Improved methods of electroporation were developed to enable the routine transformation of L. fermentum BR11 strain with the broad host range plasmid pNZ17. This recombinantly modified Lactobacillus strain was shown to possess good segregational stability over 120 generations in the absence of antibiotic selection. When this recombinant L. fermentum strain was administered to the vaginal tract of three guinea-pigs it persisted for only 5 days. Despite the relatively short period of persistence in these initial experiments, this novel vaccine approach could provide an effective means of stimulating mucosal immunity in the female genital tract.

journal_name

J Med Microbiol

authors

Rush CM,Hafner LM,Timms P

doi

10.1099/00222615-41-4-272

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

272-8

issue

4

eissn

0022-2615

issn

1473-5644

journal_volume

41

pub_type

杂志文章
  • Antimicrobial resistance in group B streptococcus: the Australian experience.

    abstract::Intrapartum chemoprophylaxis for pregnant group B streptococcus (GBS) carriers reduces vertical transmission, with a resultant decrease in neonatal as well as maternal morbidity from invasive GBS infection. Current Australian guidelines recommend penicillin for intrapartum prophylaxis of GBS carriers, with erythromyci...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.022616-0

    authors: Garland SM,Cottrill E,Markowski L,Pearce C,Clifford V,Ndisang D,Kelly N,Daley AJ

    更新日期:2011-02-01 00:00:00

  • The Helicobacter pylori flbA flagellar biosynthesis and regulatory gene is required for motility and virulence and modulates urease of H. pylori and Proteus mirabilis.

    abstract::Helicobacter pylori and Proteus mirabilis ureases are nickel-requiring metallo-enzymes that hydrolyse urea to NH3 and CO2. In both H. pylori and in an Escherichia coli model of H. pylori urease activity, a high affinity nickel transporter, NixA, is required for optimal urease activity, whereas the urea-dependent UreR ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-11-958

    authors: McGEE DJ,Coker C,Testerman TL,Harro JM,Gibson SV,Mobley HLT

    更新日期:2002-11-01 00:00:00

  • Predictive value of direct nitrate reductase assay and its clinical performance in the detection of multi- and extensively drug-resistant tuberculosis.

    abstract::Conventional culture and drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are laborious and time consuming. For this reason alternative rapid culture and DST techniques are urgently needed to shorten the time for drug-resistance detection. A total of 222 smear-positive sputum samples were evalu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.070219-0

    authors: Imperiale BR,Morcillo NS,Palomino JC,Vandamme P,Martin A

    更新日期:2014-04-01 00:00:00

  • Prevalence and virulence characteristics of enteroaggregative Escherichia coli in a case-control study among patients from Iran.

    abstract::Enteroaggregative Escherichia coli (EAEC) is an important agent of diarrhoeal diseases worldwide. The role of EAEC virulence factors in the clinical outcome of infection is not completely defined. This case-control study investigated the prevalence of EAEC, its virulence genes and the antimicrobial resistance profile ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000055

    authors: Bafandeh S,Haghi F,Zeighami H

    更新日期:2015-05-01 00:00:00

  • Disseminated intravascular coagulation in rabbits: synergistic activity of meningococcal endotoxin and materials egested from leucocytes containing meningococci.

    abstract::Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-9-4-451

    authors: DeVoe IW,Gilka F

    更新日期:1976-11-01 00:00:00

  • Virulent Toxoplasma gondii strain RH promotes T-cell-independent overproduction of proinflammatory cytokines IL12 and gamma-interferon.

    abstract::The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04860-0

    authors: Nguyen TD,Bigaignon G,Markine-Goriaynoff D,Heremans H,Nguyen TN,Warnier G,Delmee M,Warny M,Wolf SF,Uyttenhove C,Van Snick J,Coutelier JP

    更新日期:2003-10-01 00:00:00

  • Analysis of the effect of smoking on the buccal microbiome using next-generation sequencing technology.

    abstract:PURPOSE:This study aimed to investigate the effect of smoking on the buccal microbiome and to analyse the descriptive ability of each of the seven hypervariable regions in their 16S rRNA genes. METHODOLOGY:Microbiome compositions of 40 buccal swab samples collected from smokers (n =20) and non-smokers (n =20) were det...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001003

    authors: Karabudak S,Ari O,Durmaz B,Dal T,Basyigit T,Kalcioglu MT,Durmaz R

    更新日期:2019-08-01 00:00:00

  • High frequency of cultivable human subgroup F adenoviruses in stool samples from a paediatric population admitted to hospital with acute gastroenteritis.

    abstract::The family Adenoviridae consists of five genera of which the genus Mastadenovirus includes human viruses classified into 57 serotypes clustered into seven subgroups (A-G). Serotypes 40 and 41 (subgroup F) are specifically associated with childhood gastroenteritis and are the most common cause of acute gastroenteritis ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.072413-0

    authors: Arcangeletti MC,Germini D,Martorana D,Rodighiero I,De Conto F,Medici MC,Chezzi C,Calderaro A

    更新日期:2014-06-01 00:00:00

  • Antibiotic susceptibility and molecular mechanisms of macrolide resistance in streptococci isolated from adult cystic fibrosis patients.

    abstract::The cystic fibrosis (CF) airways are colonized by polymicrobial communities with high bacterial load and are influenced by frequent antibiotic exposures. This community includes diverse streptococci, some of which have been directly or indirectly associated with pulmonary exacerbations. As many streptococci are natura...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000172

    authors: Thornton CS,Grinwis ME,Sibley CD,Parkins MD,Rabin HR,Surette MG

    更新日期:2015-11-01 00:00:00

  • Host- and pathogen-dependent susceptibility and predisposition to dermatophytosis.

    abstract::Dermatophytes are a highly specialized group of keratinophilic and keratinolytic filamentous fungi causing a ringworm disease called dermatophytosis or superficial mycoses. Although dermatophyte infections do not threaten the host's life, they lower its quality in humans by causing discomfort related to cosmetic probl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.000982

    authors: Gnat S,Nowakiewicz A,Łagowski D,Zięba P

    更新日期:2019-06-01 00:00:00

  • In vitro assessment of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies.

    abstract::The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45911-0

    authors: Ikeda-Dantsuji Y,Konomi I,Nagayama A

    更新日期:2005-04-01 00:00:00

  • Role of the ABC transporter TruMDR2 in terbinafine, 4-nitroquinoline N-oxide and ethidium bromide susceptibility in Trichophyton rubrum.

    abstract::A single-copy gene, designated TruMDR2, encoding an ATP-binding cassette (ABC) transporter was cloned and sequenced from the dermatophyte Trichophyton rubrum. The ORF of TruMDR2 was 4048 nt and the deduced amino acid sequence showed high homology with ABC transporters involved in drug efflux in other fungi. The encode...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46522-0

    authors: Fachin AL,Ferreira-Nozawa MS,Maccheroni W,Martinez-Rossi NM

    更新日期:2006-08-01 00:00:00

  • Correlation between serum reactivity to Demodex-associated Bacillus oleronius proteins, and altered sebum levels and Demodex populations in erythematotelangiectatic rosacea patients.

    abstract::Rosacea is a chronic inflammatory condition that affects the skin of the face and the eyes. The aetiology of rosacea is not clearly established but increasing evidence suggests a potential role for bacteria in the induction of the condition. A role for Bacillus oleronius, originally isolated from within a Demodex foll...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.065136-0

    authors: Jarmuda S,McMahon F,Żaba R,O'Reilly N,Jakubowicz O,Holland A,Szkaradkiewicz A,Kavanagh K

    更新日期:2014-02-01 00:00:00

  • Differentiation of strains of Mycoplasma fermentans from various sources by pyrolysis mass spectrometry.

    abstract::Mycoplasma fermentans has attracted much interest both as a cofactor for the progression of AIDS and as a pathogenic agent in non-AIDS related diseases. Previous studies with serological and genetic techniques suggest that M. fermentans represents a homogeneous group of organisms, with no significant differences ident...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-4-348

    authors: Hannan PC,Kearns AM,Sisson PR,Freeman R

    更新日期:1997-04-01 00:00:00

  • Isolation of Carnobacterium sp. from a human blood culture.

    abstract::Carnobacterium species have been isolated from the environment and are not regarded as human pathogens, although they are known to cause disease in fish. Only two reports describing isolation of Carnobacterium species from human pus were found in the literature. We report what we believe to be the first isolation of C...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016808-0

    authors: Hoenigl M,Grisold AJ,Valentin T,Leitner E,Zarfel G,Renner H,Krause R

    更新日期:2010-04-01 00:00:00

  • Ribotype differences between clinical and environmental isolates of Burkholderia pseudomallei.

    abstract::Burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. However, recent surveys in Thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. To determine whether strai...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-7-565

    authors: Trakulsomboon S,Dance DA,Smith MD,White NJ,Pitt TL

    更新日期:1997-07-01 00:00:00

  • Catheter-associated bloodstream infection caused by Leifsonia aquatica in a haemodialysis patient: a case report.

    abstract::Leifsonia aquatica is an aquatic coryneform rod that is capable of forming biofilms in environmental water sources. It has rarely been associated with human infections and its pathogenicity and clinical significance are uncertain. We describe a case of catheter-related bloodstream infection in a haemodialysis patient....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.037457-0

    authors: Porte L,Soto A,Andrighetti D,Dabanch J,Braun S,Saldivia A,Flores JC,Wozniak A,García P,Weitzel T

    更新日期:2012-06-01 00:00:00

  • Streptococcus mutans infective endocarditis complicated by vertebral discitis following dental treatment without antibiotic prophylaxis.

    abstract::We report what we believe is the first reported case of Streptococcus mutans endocarditis complicated by vertebral discitis. The case is particularly interesting and topical as it occurred in a patient with pre-existing cardiac valvular disease who had recently had a dental procedure without antibiotic prophylaxis fol...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.020974-0

    authors: Biswas S,Bowler ICJW,Bunch C,Prendergast B,Webster DP

    更新日期:2010-10-01 00:00:00

  • Identification and evaluation of LPS antigen for serodiagnosis of uveitis associated with leptospirosis.

    abstract::Leptospirosis is a widespread zoonotic disease that affects all mammals in different parts of the world. Though there are many commercial kits available for the diagnosis of systemic leptospirosis, the nature of the antigen has not been described. Therefore, identification of a specific antigen is important. Since ocu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05120-0

    authors: Priya CG,Bhavani K,Rathinam SR,Muthukkaruppan VR

    更新日期:2003-08-01 00:00:00

  • Antibacterial activity of a 1,8-naphthyridine quinolone, PD 131628.

    abstract::A new 1,8-naphthyridine quinolone antimicrobial, PD131628, was found to be as, or more active than ciprofloxacin or ofloxacin against Escherichia coli and considerably more active than the two 4-quinolone agents against staphylococci in terms of both MIC and OBC (the optimum bactericidal concentration, at which the ra...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-5-353

    authors: Lewin CS

    更新日期:1992-05-01 00:00:00

  • Cross-reactions in cell-mediated immunity to Salmonella causing enteric fever.

    abstract::Cross-reactivity in a delayed-type hypersensitivity (DH) response was studied in mice immunised with live Salmonella typhi, S. paratyphi A and S. paratyphi B. Extensive cross-reactions outside the serogroup limits were observed. The ability of DH cross-reacting and non-cross-reacting sonicates to generate activated ma...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-3-233

    authors: Tiwari H,Kamat RS

    更新日期:1986-05-01 00:00:00

  • Epidemiology of culture-confirmed infections of Streptococcus pneumoniae (2012-2015) and nasopharyngeal carriage in children and households in Taiwan (2014-2015).

    abstract:PURPOSE:An observational study was performed to investigate the carriage rate and serotypes of Streptococcus pneumoniae in the 13-valent pneumococcal conjugate vaccine (PCV13) era in Taiwan. METHODOLOGY:From March 2014 to March 2015 a total of 500 healthy children and their households (631 adults) were enrolled from t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000488

    authors: Janapatla RP,Su LH,Chen HH,Chang HJ,Tsai TC,Chen PY,Chen CL,Chiu CH

    更新日期:2017-06-01 00:00:00

  • Immunological characterisation of rubella virion polypeptides.

    abstract::Antibodies specific for rubella virion polypeptide, VPI, secreted by clones of hybridoma cells or produced in rabbits in response to specific antigenic stimulation, located determinants for haemagglutination inhibiting (HI) and neutralising (Nt) antibodies on this envelope component. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-17-1-105

    authors: Ho-Terry L,Cohen A,Tedder RS

    更新日期:1984-02-01 00:00:00

  • Effects of mucopolysaccharides on penicillin-induced lysis of Staphylococcus aureus.

    abstract::Effects of four mucopolysaccharides and dextran sulphate on penicillin-induced lysis of Staphylococcus aureus FDA 209P were studied. Heparin and dextran sulphate inhibited lysis, whereas hyaluronic acid enhanced it. Chondroitin sulphates A and C had no effect. Incubation of S. aureus suspended in 0.03 M phosphate buff...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-24-4-325

    authors: Kiriyama T,Miyake Y,Sugai M,Kobayashi K,Yoshiga K,Takada K,Suginaka H

    更新日期:1987-12-01 00:00:00

  • Development of imipenem resistance in an Aeromonas veronii biovar sobria clinical isolate recovered from a patient with cholangitis.

    abstract::Several imipenem-susceptible and -resistant Aeromonas veronii biovar sobria isolates with different morphologies and antimicrobial susceptibilities recovered from bile samples of a patient with cholangitis were analysed. These isolates belonged to the same clone and the imipenem-resistant strains showed overexpression...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47804-0

    authors: Sánchez-Céspedes J,Figueras MJ,Aspiroz C,Aldea MJ,Toledo M,Alperí A,Marco F,Vila J

    更新日期:2009-04-01 00:00:00

  • Delineation of Stenotrophomonas maltophilia isolates from cystic fibrosis patients by fatty acid methyl ester profiles and matrix-assisted laser desorption/ionization time-of-flight mass spectra using hierarchical cluster analysis and principal component

    abstract::Stenotrophomonas maltophilia is an opportunist multidrug-resistant pathogen that causes a wide range of nosocomial infections. Various cystic fibrosis (CF) centres have reported an increasing prevalence of S. maltophilia colonization/infection among patients with this disease. The purpose of this study was to assess s...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.076950-0

    authors: Vidigal PG,Mosel F,Koehling HL,Mueller KD,Buer J,Rath PM,Steinmann J

    更新日期:2014-12-01 00:00:00

  • Bacteriophages in Helicobacter (Campylobacter) pylori.

    abstract::Bacteriophages in different stages of maturation were found in thin sections of a clinical isolate of Helicobacter (Campylobacter) pylori. Mature phage heads measured 70 x 60 nm and the tail at least 120 nm. Lysogeny was maintained during subculture on blood agar for more than 3 months after isolation from a gastric b...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-32-2-101

    authors: Schmid EN,von Recklinghausen G,Ansorg R

    更新日期:1990-06-01 00:00:00

  • Campylobacter jejuni adapts to aerobic metabolism in the environment.

    abstract::Campylobacter jejuni, when left on blood agar for prolonged periods, was found to survive better in air than under micro-aerobic conditions. After a period of 2-3 days in air, all strains of C. jejuni examined grew freely in air on subculture, and could be further subcultured apparently indefinitely in air. This adapt...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-38-2-145

    authors: Jones DM,Sutcliffe EM,Rios R,Fox AJ,Curry A

    更新日期:1993-02-01 00:00:00

  • Mycobacterium tuberculosis co-existence with humans: making an imprint on the macrophage P2X(7) receptor gene?

    abstract::The development of tuberculosis (TB) infection in humans depends on the mycobacterial strain and the human host, and is multigenically controlled in both. ATP ligation of P2X(7) receptors expressed on human macrophages infected with mycobacteria induces cell death and subsequent loss of intracellular bacterial viabili...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47455-0

    authors: Mokrousov I,Sapozhnikova N,Narvskaya O

    更新日期:2008-05-01 00:00:00

  • Klebsiella pneumoniae meningitis induces memory impairment and increases pro-inflammatory host response in the central nervous system of Wistar rats.

    abstract::Klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. The invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. The aim of the present study was to evalu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.063289-0

    authors: Barichello T,Simões LR,Valvassori SS,Generoso JS,Aveline PEDV,Dominguini D,Elias SG,Vilela MC,Quevedo J,Teixeira AL

    更新日期:2014-01-01 00:00:00