Rapid and accurate identification of species belonging to the Candida parapsilosis complex by real-time PCR and melting curve analysis.

abstract：:Seven isolates of B. piliformis, the agent of Tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on BRL 3A rat-hepatocyte and 3T3 mouse-fibroblast cell lines. One isolate exhibited cytopathic effects on BRL 3A cells, but not on 3T3 cells. Three ot...

journal_title：Journal of medical microbiology

authors： Riley LK,Caffrey CJ,Musille VS,Meyer JK

更新日期：1992-07-01 00:00:00

abstract：:A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated...

journal_title：Journal of medical microbiology

authors： Pourshafie MR,Grimont F,Saifi M,Grimont PAD

更新日期：2000-12-01 00:00:00

abstract：:The encapsulated strain M of Staphylococcus aureus and the encapsulated strain 76 of S. simulans survived incubation with bovine neutrophils in media containing heated bovine serum 0.5% v/v or milk whey 10% v/v. With two unencapsulated strains of S. aureus, M12 and M60, less than 5% of the original inoculum survived. ...

journal_title：Journal of medical microbiology

authors： Anderson JC,Williams MR

更新日期：1985-12-01 00:00:00

abstract：:The National Institute of Care Excellence (NICE) 2007 guidance CG54, on urinary tract infection (UTI) in children, states that clinicians should use urgent microscopy and culture as the preferred method for diagnosing UTI in the hospital setting for severe illness in children under 3 years old and from the GP setting ...

journal_title：Journal of medical microbiology

authors： McNulty CAM,Verlander NQ,Moore PCL,Larcombe J,Dudley J,Banerjee J,Jadresic L

更新日期：2015-09-01 00:00:00

abstract：:Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...

journal_title：Journal of medical microbiology

authors： Sakamoto M,Takeuchi Y,Umeda M,Ishikawa I,Benno Y

更新日期：2003-01-01 00:00:00

abstract：:Microsporum canis is the most common species isolated from canine and feline dermatophytosis in the world. However, this study reports a rare case of canine dermatophytosis caused by the anthropophilic dermatophyte Trichophyton tonsurans in the city of Fortaleza, Ceará, Brazil. The fungal characterization was performe...

journal_title：Journal of medical microbiology

authors： Brilhante RSN,Cordeiro RA,Gomes JMF,Sidrim JJC,Rocha MFG

更新日期：2006-11-01 00:00:00

abstract：:Helicobacter pylori is believed to trigger neutrophil activation through several factors, including the H. pylori neutrophil-activating protein (HpNAP). The aim of this study was to characterise the factors within H. pylori cell-free extracts that stimulate neutrophil activation. Neutrophil activation was found to be ...

journal_title：Journal of medical microbiology

authors： Leakey A,Hirst R,LA Brooy J

更新日期：2001-09-01 00:00:00

abstract：:Introduction.Serratia marcescens is a bacterial pathogen that causes ventilator-associated pneumonia and ocular infections. The FlhD and FlhC proteins complex to form a heteromeric transcription factor whose regulon, in S. marcescens, regulates genes for the production of flagellum, phospholipase A and the cytolysin S...

journal_title：Journal of medical microbiology

authors： Stella NA,Brothers KM,Shanks RMQ

更新日期：2020-12-10 00:00:00

abstract：:Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...

journal_title：Journal of medical microbiology

authors： Barketi-Klai A,Hoys S,Lambert-Bordes S,Collignon A,Kansau I

更新日期：2011-08-01 00:00:00

abstract：:Faecal specimens from 843 cases of diarrhoea in the community were tested for the presence of Clostridium difficile cytotoxin and Clostridium perfringens enterotoxin. C. difficile cytotoxin was detected in faecal specimens from 0.6 % of cases aged at least 2 years by using a Vero cell assay. Factors associated with de...

journal_title：Journal of medical microbiology

authors： Forward LJ,Tompkins DS,Brett MM

更新日期：2003-09-01 00:00:00

abstract：:Effects on growth and toxin A production of sub-MIC concentrations of six different antibiotics were investigated in three strains of Clostridium difficile: reference strain NCTC 11223, a fully sequenced strain (630) and a locally endemic isolate (strain 338a). The antibiotics chosen for investigation were the agents ...

journal_title：Journal of medical microbiology

authors： Drummond LJ,Smith DGE,Poxton IR

更新日期：2003-12-01 00:00:00

abstract：:Sixty-three Staphylococcus aureus strains (40 from clinical sources and 23 from food sources) were examined for toxic shock syndrome toxin-1 (TSST-1) using PCR, phage typed using the international phage set (IPS) and tested for their susceptibility to antibiotics. Only three strains (all from clinical sources) were po...

journal_title：Journal of medical microbiology

authors： El-Ghodban A,Ghenghesh KS,Márialigeti K,Esahli H,Tawil A

更新日期：2006-02-01 00:00:00

abstract：PURPOSE:The aim of this study was to assess the biotope of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast, and clarify the possible epidemiological relationship between environmental and clinical strains. METHODOLOGY:Samples from Eucalyptus camaldulensis (n=136), Mangifera indica (n=1...

journal_title：Journal of medical microbiology

authors： Kassi FK,Bellet V,Drakulovski P,Krasteva D,Roger F,Valérie BA,Aboubakar T,Doumbia A,Kouakou GA,Delaporte E,Reynes J,Yavo W,Menan HIE,Bertout S

更新日期：2018-01-01 00:00:00

abstract：:The therapeutic alternatives available for use against ciprofloxacin-resistant enteric fever isolates in an endemic area are limited. The antibiotics currently available are the quinolones, third-generation cephalosporins and conventional first-line drugs. In this study, the MICs of various newer drugs were determined...

journal_title：Journal of medical microbiology

authors： Capoor MR,Rawat D,Nair D,Hasan AS,Deb M,Aggarwal P,Pillai P

更新日期：2007-11-01 00:00:00

abstract：:The bactericidal activity of compound DR-3355, an optically active isomer of ofloxacin, was measured against Escherichia coli, Staphylococcus aureus and S. epidermidis, in nutrient broth and in phosphate-buffered saline. DR-3355 was found to be approximately twice as active as ofloxacin in terms of the concentration a...

journal_title：Journal of medical microbiology

authors： Lewin CS,Amyes SG

更新日期：1989-11-01 00:00:00

abstract：:A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foo...

journal_title：Journal of medical microbiology

authors： Demirkan I,Carter SD,Winstanley C,Bruce KD,McNAIR NM,Woodside M,Hart CA

更新日期：2001-12-01 00:00:00

abstract：:This study analysed the characteristics and genetic similarity of recent Klebsiella pneumoniae carbapenemase (KPC-2)-producing Klebsiella pneumoniae isolates from Korea. Recent laboratory surveillance detected an increase in carbapenemase-producing Enterobacteriaceae in Korea. A total of 6 KPC-2-producing K. pneumonia...

journal_title：Journal of medical microbiology

authors： Yoo JS,Kim HM,Yoo JI,Yang JW,Kim HS,Chung GT,Lee YS

更新日期：2013-09-01 00:00:00

abstract：:A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal traini...

journal_title：Journal of medical microbiology

authors： Morais L,Carvalho MDG,Roca A,Flannery B,Mandomando I,Soriano-Gabarró M,Sigauque B,Alonso P,Beall B

更新日期：2007-09-01 00:00:00

abstract：PURPOSE:Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. pararugosa species. METH...

journal_title：Journal of medical microbiology

authors： Peremalo T,Madhavan P,Hamzah S,Than L,Wong EH,Nasir MDM,Chong PP,Ng KP

更新日期：2019-03-01 00:00:00

abstract：:Human gastric epithelial immortalized GES-1 cells were infected with spiral and coccoid Helicobacter pylori. Scanning electron microscopy was used to determine the ability of the two forms of H. pylori to adhere to GES-1 cells. GES-1 cell apoptosis induced by coccoid and spiral H. pylori was analysed using flow cytome...

journal_title：Journal of medical microbiology

authors： Liu ZF,Chen CY,Tang W,Zhang JY,Gong YQ,Jia JH

更新日期：2006-08-01 00:00:00

abstract：:Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of Bacteroides fragilis alone or in combination with Escherichia coli was evaluated. E. coli produced much more powerful chemotactic factors than B. fragilis. The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysacchar...

journal_title：Journal of medical microbiology

authors： Namavar F,Verweij-Van Vught AM,Bal M,MacLaren DM

更新日期：1987-09-01 00:00:00

abstract：:Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but not for the multiplication of Giardia duodenalis in the intestinal lumen. The microbial components responsible for this phenomenon are not known. Twenty-eight facultative and three strictly anaerobic micro-organisms were is...

journal_title：Journal of medical microbiology

authors： Torres MF,Uetanabaro APT,Costa AF,Alves CA,Farias LM,Bambirra EA,Penna FJ,Vieira EC,Nicoli JR

更新日期：2000-03-01 00:00:00

abstract：:Invasive infections caused by Brevundimonas vesicularis are very rare in humans. We experienced an unusual case of liver abscess due to B. vesicularis in an immunocompetent young male. The patient was successfully treated by liver abscess drainage and with antimicrobial therapy of ceftriaxone followed by ampicillin/su...

journal_title：Journal of medical microbiology

authors： Yoo SH,Kim MJ,Roh KH,Kim SH,Park DW,Sohn JW,Yoon YK

更新日期：2012-10-01 00:00:00

abstract：PURPOSE:We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and the identification of fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). METHODOLOGY:Fifty cases of ROCM were included in the study. Conventional identification was performed using micros...

journal_title：Journal of medical microbiology

authors： Zaman K,Rudramurthy SM,Das A,Panda N,Honnavar P,Kaur H,Chakrabarti A

更新日期：2017-08-01 00:00:00

abstract：:Human brucellosis is a zoonotic disease which is endemic in Saudi Arabia. The aim of this study was to investigate the humoral immune responses and identify the target antigens that persist at different stages in human brucellosis during antibiotic therapy. To do this, an acute case of accidental nosocomial infection ...

journal_title：Journal of medical microbiology

authors： Kwaasi AA,Al-Mohanna FA,Nakeeb SM,Roberts GT,Al-Thawadi S,Hassan AY,Al-Hokail A,Elfaki MG

更新日期：2005-06-01 00:00:00

abstract：:Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without...

journal_title：Journal of medical microbiology

authors： Menon S,Stansfield SH,Logan B,Hocking JS,Timms P,Rombauts L,Allan JA,Huston WM

更新日期：2016-09-01 00:00:00

abstract：:Historically, enteropathogenic Escherichia coli (EPEC) are a well-known cause of outbreaks of infantile diarrhoea associated with morbidity and mortality in England. The aim of this study was to provide an update on the microbiology and epidemiology of strains of EPEC in England between 2010 and 2012. A wide range of ...

journal_title：Journal of medical microbiology

authors： Sakkejha H,Byrne L,Lawson AJ,Jenkins C

更新日期：2013-10-01 00:00:00

abstract：:We describe the case of a graft versus host disease (GvHD) patient, in whom Hafnia alvei was cultured as a single organism, and at high bacterial counts from stool samples, from the onset of the disease until its resolution. This case is a further example of the contentious role of this species in causing human intest...

journal_title：Journal of medical microbiology

authors： Savini V,Di Bartolomeo E,Catavitello C,Talia M,Manna A,Febbo F,Balbinot A,Di Bonaventura G,Di Bartolomeo P,Piccolomini R,D'Antonio D

更新日期：2008-09-01 00:00:00

abstract：:The effect of glucan on Leishmania major infection was studied in BALB/c mice, which are highly susceptible to leishmania infection. Glucan (0.45 mg), or isovolumetric dextrose, was administered intraperitoneally 7, 5, 3 and 1 day before infection with L. major promastigotes. At 3, 5, 6, 8 and 10 weeks after infection...

journal_title：Journal of medical microbiology

authors： Al Tuwaijri AS,Mahmoud AA,Al Mofleh IA,Al Khuwaitir SA

更新日期：1987-06-01 00:00:00

abstract：:The characterization of integrons and their promoters in 156 antibiotic-resistant clinical isolates of the important zoonotic pathogens Salmonella enterica subsp. enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) from Norwegian hospitals were performed. Integrons were found in 64 of 66 S....

journal_title：Journal of medical microbiology

authors： Lindstedt BA,Heir E,Nygård I,Kapperud G

更新日期：2003-02-01 00:00:00