Role of swarming in the formation of crystalline Proteus mirabilis biofilms on urinary catheters.

Abstract:

:The care of many patients undergoing long-term bladder catheterization is frequently complicated by infection with Proteus mirabilis. These organisms colonize the catheter, forming surface biofilm communities, and their urease activity generates alkaline conditions under which crystals of magnesium ammonium phosphate and calcium phosphate are formed and become trapped in the biofilm. As the biofilm develops it obstructs the flow of urine through the catheter, causing either incontinence due to leakage of urine around the catheter or retention of urine in the bladder. The aim of this study was to investigate the role of the surface-associated swarming motility of P. mirabilis in the initiation and development of these crystalline catheter biofilms. A set of stable transposon mutants with a range of swimming and swarming abilities were tested for their ability to colonize silicone surfaces in a parallel-plate flow cell. A laboratory model of the catheterized bladder was then used to examine their ability to form crystalline, catheter-blocking biofilms. The results showed that neither swarming nor swimming motility was required for the attachment of P. mirabilis to silicone. Mutants deficient in swarming and swimming were also capable of forming crystalline biofilms and blocking catheters more rapidly than the wild-type strain.

journal_name

J Med Microbiol

authors

Jones BV,Mahenthiralingam E,Sabbuba NA,Stickler DJ

doi

10.1099/jmm.0.46123-0

keywords:

subject

Has Abstract

pub_date

2005-09-01 00:00:00

pages

807-813

issue

Pt 9

eissn

0022-2615

issn

1473-5644

journal_volume

54

pub_type

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