Observations on the growth and movement of Acinetobacter on semi-solid media.

abstract：PURPOSE:From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidiscipl...

journal_title：Journal of medical microbiology

authors： Duranti A,Sabbatucci M,Blasi G,Acciari VA,Ancora M,Bella A,Busani L,Centorame P,Cammà C,Conti F,De Medici D,Di Domenico M,Di Marzio V,Filippini G,Fiore A,Fisichella S,Gattuso A,Gianfranceschi M,Graziani C,Guidi F,

更新日期：2018-09-01 00:00:00

abstract：PURPOSE:To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients. METHODOLOGY:Clinical and demographic data were registered prospectively. Phylogenetic background of K. pneumoniae isolates was analysed by PCR melting pro...

journal_title：Journal of medical microbiology

authors： Gołębiewska JE,Krawczyk B,Wysocka M,Ewiak A,Komarnicka J,Bronk M,Rutkowski B,Dębska-Ślizień A

更新日期：2019-03-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is suitable for high-throughput and rapid microbial diagnosis at relatively low cost and can be considered an alternative to conventional biochemical and molecular identification systems in clinical microbiological laboratories...

journal_title：Journal of medical microbiology

authors： Jamal WY,Shahin M,Rotimi VO

更新日期：2013-04-01 00:00:00

abstract：:A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (tst). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test...

journal_title：Journal of medical microbiology

authors： Shimaoka M,Yoh M,Takarada Y,Yamamoto K,Honda T

更新日期：1996-03-01 00:00:00

abstract：PURPOSE:Catheter-related bloodstream infections (CRBSIs) are among the most common hospital-acquired infections. We aimed to survey methicillin resistance, biofilm production and susceptibility to vancomycin, linezolid and other antibiotics for staphylococci isolated from CRBSIs. METHODOLOGY:Fifty-eight isolates [20 S...

journal_title：Journal of medical microbiology

authors： Hashem AA,Abd El Fadeal NM,Shehata AS

更新日期：2017-06-01 00:00:00

abstract：:The activities of three sulphonamides and trimethoprim against strains of Pseudomonas aeruginosa have been studied. Sulphadiazine had most activity, sulphadimidine had little, and the activity of sulphamethoxazole was intermediate. According to their sensitivity to sulphamethoxazole, strains were divided into two grou...

journal_title：Journal of medical microbiology

authors： Grey D,Hamilton-Miller JM

更新日期：1977-08-01 00:00:00

abstract：:Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne infectious diseases caused by Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato species, respectively. In this study, p44/msp2 paralogues specific to A. phagocytophilum and 5S-23S rRNA gene-intergenic spacers specific to B. burg...

journal_title：Journal of medical microbiology

authors： Masuzawa T,Kharitonenkov IG,Okamoto Y,Fukui T,Ohashi N

更新日期：2008-08-01 00:00:00

abstract：:To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...

journal_title：Journal of medical microbiology

authors： Sugawara I,Yamada H,Kazumi Y,Doi N,Otomo K,Aoki T,Mizuno S,Udagawa T,Tagawa Y,Iwakura Y

更新日期：1998-10-01 00:00:00

abstract：:Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fl...

journal_title：Journal of medical microbiology

authors： De Graaf-Miltenburg LA,Van Vliet KE,Ten Hagen TL,Verhoef J,Van Strijp JA

更新日期：1994-01-01 00:00:00

abstract：:The abilities of six strains of Salmonella typhimurium to associate with rabbit ileal mucosa have been measured in vitro. Two were "virulent" strains (TML and W118 which are invasive and inducers of fluid secretion in rabbit ileal loops); four were "avirulent" (LT7, M206 and SL1027 which are invasive but induce neglig...

journal_title：Journal of medical microbiology

authors： Worton KJ,Candy DC,Wallis TS,Clarke GJ,Osborne MP,Haddon SJ,Stephen J

更新日期：1989-08-01 00:00:00

abstract：:A periplasmic flagellin gene, flaB, of Leptospira interrogans serovar pomona (strain Pomona) was expressed in Escherichia coli for the production and antigenic characterisation of the protein. The flaB structural gene, which was previously cloned into pUC118, was derived by PCR from the recombinant plasmid and used to...

journal_title：Journal of medical microbiology

authors： Lin M,Bughio N,Surujballi O

更新日期：1999-11-01 00:00:00

abstract：:Listeria monocytogenes cells are ingested by protozoa such as Acanthamoeba sp. or Tetrahymena pyriformis. However, they are not killed, but survive within the protozoa and may multiply intracellularly. The protozoa are lysed within about 8 days, releasing viable L. monocytogenes. No co-existence was observed between L...

journal_title：Journal of medical microbiology

authors： Ly TM,Müller HE

更新日期：1990-09-01 00:00:00

abstract：:Cryptococcosis causes disseminated disease in AIDS patients. In contrast to what occurs in laboratory conditions, a large capsule is produced by Cryptococcus neoformans in vivo during infection. The aim of this study was to compare the in vitro activity of different antifungal agents against 34 clinical isolates of C....

journal_title：Journal of medical microbiology

authors： Vitale RG,Pascuccelli V,Afeltra J

更新日期：2012-03-01 00:00:00

abstract：:Resistance patterns and carbapenemase gene presence among Klebsiella pneumoniae isolates from the University General Hospital of Patras, Greece during a ten-year period were analysed under a surveillance programme for multi-drug-resistant bacteria. From 2005 to 2014, K. pneumoniae isolates from clinically significant ...

journal_title：Journal of medical microbiology

authors： Spyropoulou A,Papadimitriou-Olivgeris M,Bartzavali C,Vamvakopoulou S,Marangos M,Spiliopoulou I,Anastassiou ED,Christofidou M

更新日期：2016-03-01 00:00:00

abstract：:The potential of human plasma (HP) or serum (HS) as a replacement for fetal calf serum (FCS) was evaluated in a liver infusion tryptose (LIT) medium for bulk cultivation of Leishmania donovani promastigotes. The promastigote yield with the LIT-FCS standard medium was 0.4-1.8×10(7) ml(-1), and yields of 0.5-3.4×10(7) (...

journal_title：Journal of medical microbiology

authors： Mahamoud A,Osman HA,Mansour D,El Harith A

更新日期：2013-08-01 00:00:00

abstract：:Catechin, a constituent of tea, possesses various bioactivities. In particular, the most abundant catechin in tea is epigallocatechin gallate (EGCg), which has an anti-inflammatory effect. In the present study, the usability of EGCg for osteomyelitis treatment was examined. Osteomyelitis is a difficult disease to cure...

journal_title：Journal of medical microbiology

authors： Ishida I,Kohda C,Yanagawa Y,Miyaoka H,Shimamura T

更新日期：2007-08-01 00:00:00

abstract：:Phenotypic loss of protein A production was tested in six methicillin-resistant (McR) Staphylococcus aureus (MRSA) isolates and their isogenic methicillin-sensitive (McS) variants by a radiolabelled IgG-binding assay with washed cells and by Western blotting of supernates prepared from lysed washed cells. Genomic DNA ...

journal_title：Journal of medical microbiology

authors： Poston SM,Glancey GR,Wyatt JE,Hogan T,Foster TJ

更新日期：1993-12-01 00:00:00

abstract：:The rat was evaluated as an experimental model for disseminated candidosis by quantitating blood clearance and initial organ localisation of 3H-leucine-labelled Candida albicans after intravenous injection into the tail or portal vein. Viable or formalin-killed blastoconidia or viable blastoconidia with germ tubes wer...

journal_title：Journal of medical microbiology

authors： Katz S,Grosfeld JL,Folkening WJ,Rosenthal RS,Merkel GJ

更新日期：1992-10-01 00:00:00

abstract：:The aim of this study was to evaluate an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-Helicobacter pylori specific IgG antibodies in specimens of oral fluid. Antral biopsy specimens, serum and oral fluid samples were collected from 81 patients attending for upper gastrointestinal endoscopy. The ...

journal_title：Journal of medical microbiology

authors： Marshall B,Howat AJ,Wright PA

更新日期：1999-11-01 00:00:00

abstract：PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...

journal_title：Journal of medical microbiology

authors： Rupp S,Bärtschi M,Frey J,Oevermann A

更新日期：2017-07-01 00:00:00

abstract：:Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enter...

journal_title：Journal of medical microbiology

authors： Paciorek J

更新日期：2002-07-01 00:00:00

abstract：:The present study aimed to evaluate the role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of Candida albicans and non-albicans Candida (NAC) spp. Biofilm formation was determined by microtitre plate assay and bright-field and scanning electron microscopy. The matrix ...

journal_title：Journal of medical microbiology

authors： Kumari A,Mankotia S,Chaubey B,Luthra M,Singh R

更新日期：2018-06-01 00:00:00

abstract：:Clostridium perfringens type A produces an enterotoxin that induces diarrhoea experimentally in man and animals. The enterotoxin causes increased membrane permeability in susceptible cells which is thought to be due to pore formation in the host cell membrane. The effect of purified C. perfringens enterotoxin on intac...

journal_title：Journal of medical microbiology

authors： Hardy SP,Denmead M,Parekh N,Granum PE

更新日期：1999-03-01 00:00:00

abstract：:Sixty-one isolates of Candida albicans were tested for their phospholipase activity and this parameter was correlated with pathogenicity in albino mice. Of the isolates tested, 57 (93%) showed appreciable phospholipase activity and of these, 55 (90%) were pathogenic to mice. A significant correlation was found between...

journal_title：Journal of medical microbiology

authors： Kothavade RJ,Panthaki MH

更新日期：1998-02-01 00:00:00

abstract：:The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products ...

journal_title：Journal of medical microbiology

authors： Riggio MP,Lennon A

更新日期：1999-03-01 00:00:00

abstract：:Polyacrylamide-agarose gel electrophoresis was used to study polymorphism of lactate dehydrogenase (LDH), nucleoside phosphorylase (NSP), phosphoglucose isomerase (PGI), hydroxybutyrate dehydrogenase (HBD), adenylate kinase (ADK) and esterases of 44 strains of Streptococcus pyogenes, 25 group G streptococcal strains, ...

journal_title：Journal of medical microbiology

authors： Bert F,Picard B,Lambert-Zechovsky N,Goullet P

更新日期：1995-06-01 00:00:00

abstract：:A total of 2401 isolates of Haemophilus parainfluenzae was isolated from respiratory secretions of 36 healthy adults and 128 patients with chronic bronchitis over a period of 1 year. The isolates were allocated to eight biotypes, by their production of indole, urease and ornithine decarboxylase. Biotypes I and II cons...

journal_title：Journal of medical microbiology

authors： Taylor DC,Cripps AW,Clancy RL,Murree-Allen K,Hensley MJ,Saunders NA,Sutherland DC

更新日期：1992-04-01 00:00:00

abstract：:Prevotella intermedia and the newly described P. nigrescens cannot be reliably distinguished by phenotypic tests. In this study, restriction endonuclease digestion of amplified 16S rDNA (16S rDNA PCR-RFLP) was used to generate restriction profiles of the type strains of P. intermedia and P. nigrescens and 43 fresh iso...

journal_title：Journal of medical microbiology

authors： Milsom SE,Sprague SV,Dymock D,Weightman AJ,Wade WG

更新日期：1996-01-01 00:00:00

abstract：:Cell-surface hydrophobicity of Helicobacter (formerly Campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. The hydrophobicity of H. pylori greatly exceeded that of Campylobacter fetus subsp. fetus, C. jejuni and Bacillus subtilis. A partition coefficient (PC) o...

journal_title：Journal of medical microbiology

authors： Pruul H,Goodwin CS,McDonald PJ,Lewis G,Pankhurst D

更新日期：1990-06-01 00:00:00

abstract：:The purpose of this study was to determine optimal criteria for microbiology laboratory screening of endotracheal tube (ETT) specimens submitted for bacterial culture from adult patients. ETT specimens from adult patients that were received by two microbiology laboratories were prospectively evaluated and subdivided i...

journal_title：Journal of medical microbiology

authors： Walkty A,Lagacé-Wiens PRS,Manickam K,Adam H,Pieroni P,Alfa M,Karlowsky JA

更新日期：2012-09-01 00:00:00