Abstract：
:A procedure for dissociating viable taste cells from the rodent tongue was described. After the tongue was excised, the foliate and circumvallate papillae from the posterior region were blocked to small pieces and incubated for 2-3 h in a calcium-free salt solution containing the enzyme papain. The pieces were then transferred to a Ca-replete medium. The epithelium was easily removed from the underlying muscle and gland tissue with forceps. Light mechanical agitation caused single cells, epithelial and gustatory, to dissociate from the keratinized epithelium. Taste cells were identified by their morphology. Their viability was assessed with the patch clamp technique in the whole cell recording mode. This procedure should enhance the electrophysiological study of gustatory transduction mechanisms.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Herness MSdoi
10.1016/0304-3940(89)90202-4subject
Has Abstractpub_date
1989-11-20 00:00:00pages
60-4issue
1-2eissn
0304-3940issn
1872-7972pii
0304-3940(89)90202-4journal_volume
106pub_type
杂志文章abstract：:Peptide histidine-isoleucine (PHI) immunoreactivity was located in amacrine-like cells in adult rat retina by use of immunohistochemical techniques. Immunoreactive somata were found in the proximal part of the inner nuclear layer. From these somata, processes could be followed into the inner plexiform layer. The termi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(87)90444-7
更新日期：1987-08-31 00:00:00
abstract：:Experiments involving the acute administration of a central cholinergic substance (L-acetylcarnitine) were performed on 6 healthy subjects to obtain additional evidence that the electrophysiological method developed by Bussel and Pierrot-Deseilligny in 1977 does actually assess recurrent inhibition in man. In all the ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90214-0
更新日期：1989-11-20 00:00:00
abstract：:The occurrence and distribution of nociceptin, a 17-amino acid peptide with structural similarity to dynorphin A, and its receptor, opioid receptor-like-1 (ORL1) receptor, were investigated in the guinea-pig sympathetic nervous system by means of immunohistochemistry and reverse transcription-polymerase chain reaction...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)00662-9
更新日期：1997-09-26 00:00:00
abstract：:Radiofrequency lesions of the ventral mesencephalic tegmentum (VMT), a region located above the nucleus interpeduncularis, provoked a disappearance of the spontaneous alternation behavior. The lesioned rats scores (44%) are not different from the chance level, while the alternation rate amounts to 77% in the control g...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(76)90101-4
更新日期：1976-09-01 00:00:00
abstract：:In the present experiments it was tested whether the organic calcium antagonist verapamil has an influence on N-methyl-D-aspartate (NMDA) induced cortical field potentials (CFP) in neocortical slices of guinea pigs. NMDA (1 mumol/l) was applied via a micropipette by pressure pulses. Verapamil (60 mumol/l) was administ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90288-v
更新日期：1993-10-29 00:00:00
abstract：:Molecular forms of butyrylcholinesterase (BuChE) were studied in microvessels isolated from rat brain, using sedimentation analysis in sucrose gradients. Three forms, G1, G2 and G4, were found with sedimentation coefficients close to 3S, 6S and 9S, respectively. The relative proportion of the 3 forms was 19% for the m...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(90)90164-5
更新日期：1990-11-27 00:00:00
abstract：:In a random number generation (RNG) task subjects are instructed to generate the numbers 1-10 in a random fashion. RNG performance is assumed to involve executive functions, as it requires controlled response generation and suppression of habitual responses. To investigate cerebral structures involved in RNG associate...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(03)00496-8
更新日期：2003-07-10 00:00:00
abstract：:Micro traumatic brain injury (TBI) is the most common type of brain injury, but the mechanisms underlying it are poorly understood. Aquaporin-4 (AQP4) is a water channel expressed in astrocyte end-feet, which plays an important role in brain edema. However, little is known about the role of AQP4 in micro TBI. Here, we...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2015.05.006
更新日期：2015-06-26 00:00:00
abstract：:Satellite glial cells (SGCs) surround the neurons in sympathetic ganglia and are believed to make important contributions to the function of the ganglia under normal and pathological conditions. It has been proposed that SGCs communicate chemically with the neurons, but little is known about their pharmacological prop...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2018.01.051
更新日期：2018-04-03 00:00:00
abstract：:Remacemide is a potential anticonvulsant drug with an active metabolite, desglycinyl-remacemide (DGR). Both moieties have been reported to block neuronal Na(+) channels and the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor. The effects of remacemide and DGR on zero Mg(2+)/4-aminopyridine-induced epileptifo...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(01)02511-3
更新日期：2002-03-15 00:00:00
abstract：:Effects of anxiety on perceptual-motor organisation of rhythmical forearm movements were examined using an interrupted time series design with staggered baselines. Participants were exposed to repeated baseline sessions interrupted with two anxiety-inducing sessions. Results showed that under moderate levels of anxiet...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.04.036
更新日期：2005-08-12 00:00:00
abstract：:Several studies demonstrated that Diabetes mellitus (DM) enhances the risk for Alzheimer's disease (AD). Although hyperglycemia and perturbed function of insulin signaling have been proposed to contribute to AD pathogenesis, the molecular mechanisms behind this association is not clear yet. Seladin-1 is an enzyme cata...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2016.09.018
更新日期：2016-10-28 00:00:00
abstract：:The presence of neuromelanic pigment in neurons of the rat substantia nigra (SN) is a matter of controversy. The presence of neuromelanin in the SN of 15--24-month-old rats was investigated by electron microscopy (EM) and the findings compared to the morphology and distribution of neuromelanin in SN of dogs. In the ra...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(86)90614-2
更新日期：1986-12-03 00:00:00
abstract：:The rat spinal cord slice preparation has been used to investigate the sensitivity of deep dorsal horn neurones to the excitatory amino acids N-methyl-D-aspartate (NMDA), quisqualate and L-glutamate. Intracellular recordings were made from 44 neurones in laminae III-VI of 14- to 16-day rats. Superfusion of quisqualate...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(88)90541-1
更新日期：1988-07-08 00:00:00
abstract：:Linkage studies have implicated a broad region on chromosome 10q in Alzheimer's disease (AD). A recent genetic association study has provided evidence that polymorphism in the gene encoding alpha-3 catenin (CTNNA3, referred to previously as VR22 and also known as alpha-T catenin) may underlie linkage signals. Here, to...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.01.032
更新日期：2004-04-01 00:00:00
abstract：:Accumulating evidence indicates that obesity is an independent risk factor for developing Alzheimer disease (AD). Recent studies have shown that diet-induced obesity (DIO) enhances AD-related pathologies in transgenic mouse models of the disease. DIO increases amyloid β (Aβ) deposition in amyloidogenic transgenic mice...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.04.028
更新日期：2014-06-13 00:00:00
abstract：:The cellular location of kappa-opioid binding sites in the guinea pig cerebellum has been investigated. kappa-Opioid sites were labelled using [3H]ethylketocyclazocine in the presence of mu- and delta-blocking ligands. In vitro autoradiography demonstrated that this kappa-opioid binding was localized predominantly in ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(88)90330-8
更新日期：1988-01-11 00:00:00
abstract：:Contradictory results have been reported regarding the participation of bulbospinal inspiratory (BSI) neurons in vomiting contractions of the diaphragm in dogs and cats maintained at low and high end-tidal CO2 levels, respectively. To resolve this discrepancy, effects of CO2 levels on vomiting activities were observed...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90533-9
更新日期：1994-10-24 00:00:00
abstract：:Heat shock protein 70 family was demonstrated to play a critical role in protein homeostasis, a process profoundly impaired in neurodegenerative disorders. Neurodegenerative diseases are characterized by the accumulation of different kind of proteins and the formation of insoluble aggregates which are toxic for neuron...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.135272
更新日期：2020-09-25 00:00:00
abstract：:Ethanol withdrawal is a serious clinical problem owing in part to over stimulation of ionotropic glutamate receptors in the brain and is linked to elevated oxidative damage. In this study, we tested the hypothesis that lipid peroxidation is elevated in the brain tissue of rats fed an ethanol-containing diet for 6 week...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.06.058
更新日期：2006-09-25 00:00:00
abstract：:The amino acid content of Torpedo synaptosomes was investigated. It was found that glutamate and taurine were particularly enriched, their concentration exceeding that of acetylcholine by 50-60%. Other amino acids such as alanine, gamma-aminobutyric acid (GABA), glycine, leucine and phenylalanine were also present, bu...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(87)90171-6
更新日期：1987-11-10 00:00:00
abstract：:The neuroprotective activity of riluzole has been studied on N-methyl-D-aspartate (NMDA)- or veratridine-induced toxicity in immature rat hippocampal slices. Neurodegeneration was assessed by the measurement of LDH release and histology. Veratridine-induced LDH release can be inhibited by 100 microM riluzole (-90% and...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90053-1
更新日期：1994-08-15 00:00:00
abstract：:Human low back pain sometimes originates from lumbar facet joints. In human lumbar facet joint inflammation or degeneration, the referred pain is not only expanded into the low back area but also into the leg or foot. The rat L5-L6 facet joint is innervated by the L1-L5 dorsal root ganglia. The presence of brain-deriv...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00120-9
更新日期：2002-04-26 00:00:00
abstract：:Silent synapses display no excitatory post-synaptic currents (EPSCs) at resting potentials, but can conduct at depolarized potentials. In the hippocampal CA1 region of young animals, conversion of silent synapses to functional synapses occurs rapidly after pairing post-synaptic depolarization with 1Hz pre-synaptic sti...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.02.035
更新日期：2007-04-24 00:00:00
abstract：:Both substance P (SP)- and epinephrine-containing neurons in the rostral ventrolateral medulla have been thought to play a role in regulating vasomotor tone. The combination of retrograde transport of a fluorescent dye (Fast Blue) and immunofluorescent staining for SP- and phenylethanolamine-N-methyltransferase (PNMT)...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90444-6
更新日期：1985-04-19 00:00:00
abstract：:Long-term potentiation (LTP) of perforant-path dentate granule cell synapses, in awake rats, was followed by a time-dependent expression of brain-derived neurotrophic factor (BDNF) mRNA in dentate granule cells. This BDNF expression was blocked by the N-methyl-D-aspartate (NMDA) antagonist dizocilpine maleate (MK-801)...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90420-p
更新日期：1993-10-01 00:00:00
abstract：:Recent reports have suggested that variants in the sortilin-related receptor gene (SORL1) increase the risk of late onset Alzheimer's disease (AD) in Northern European, Hispanic, African-American and Isreali-Arab populations. SORL1 directs trafficking of amyloid precursor protein (APP) and under-expression of SORL1 ma...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.08.042
更新日期：2007-09-25 00:00:00
abstract：:The expression of pre-pro-somatostatin (ppSOM) mRNA in the spinal cord of the developing rat was determined by in situ hybridization. Expression of ppSOM mRNA was detected in cells in the gray matter of the dorsal and ventral horns at day E15, the earliest stage examined in this study. Expression of ppSOM mRNA persist...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90361-n
更新日期：1993-12-12 00:00:00
abstract：:Our previous work has established that adenosine is toxic to chick embryonic sympathetic neurons and kills freshly plated neurons by a process of apoptosis. Although the exact mechanism remains unknown, we found that phosphorylation of adenosine was essential to the toxicity. Using markers for RNA ([3H]uridine) and pr...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(98)00369-3
更新日期：1998-06-05 00:00:00
abstract：:The effect of chronic salt loading on urocortin-like immunoreactivity (Ucn-IR) was investigated in the rat hypothalamo-neurohypophysial system. In control rats a few Ucn-IR neurons were observed scattered throughout the supraoptic nucleus (SON) and few in the paraventricular nucleus (PVN). A small number of Ucn-IR fib...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)00327-3
更新日期：1997-05-16 00:00:00
