Abstract:
:Several studies demonstrated that Diabetes mellitus (DM) enhances the risk for Alzheimer's disease (AD). Although hyperglycemia and perturbed function of insulin signaling have been proposed to contribute to AD pathogenesis, the molecular mechanisms behind this association is not clear yet. Seladin-1 is an enzyme catalyzing the last step in cholesterol biosynthesis converting desmosterol to cholesterol. The neuroprotective function of seladin-1 has gained interest in AD research recently. Seladin-1 has anti-apoptotic properties and regulates the expression of β-secretase (BACE-1). Here we measured seladin-1 mRNA and protein expressions in rat primary cultured neurons under diabetic conditions and also in the brains of rats with streptozotocine (STZ)-induced diabetes. We show that constant lack of insulin for 5days decreased seladin-1 levels in cultured rat primary neurons. Similarly, a decrease in seladin-1 was found in the brains of rats with STZ-induced diabetes. However, if the lack of insulin and/or high glucose treatment was intermittent, neuronal seladin-1 levels were not affected in vitro. On the other hand, treatment of neurons with metformin resulted in a significant increase in seladin-1. Constant lack of insulin for 5days, as well as high glucose treatment, increased the neuronal expression of BACE-1 in vitro, but not in the in vivo model. Our study defines insulin as a regulator of seladin-1 expression for the first time. The relevance of these findings for the association of DM with AD is discussed.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Kazkayasi I,Ismail MA,Parrado-Fernandez C,Björkhem I,Pekiner C,Uma S,Cedazo-Minguez A,Burul-Bozkurt Ndoi
10.1016/j.neulet.2016.09.018subject
Has Abstractpub_date
2016-10-28 00:00:00pages
174-181eissn
0304-3940issn
1872-7972pii
S0304-3940(16)30691-7journal_volume
633pub_type
杂志文章abstract::The intramedullary axon collateral systems of adult cat spinal alpha-motoneurones were studied with morphological and physiological techniques 12 weeks after peripheral transection of the motor axons. Morphologically, intracellular horseradish peroxidase (HRP) staining showed that the axotomy induced an elimination of...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(84)90343-4
更新日期:1984-11-23 00:00:00
abstract::We describe a new model of neurogenic pulmonary edema in spinal cord injured Wistar male rats. The pulmonary edema was elicited by an epidural thoracic balloon compression spinal cord lesion, performed under a low concentration of isoflurane (1.5 or 2%) in air. Anesthesia with 1.5% isoflurane promoted very severe inte...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.06.053
更新日期:2007-08-16 00:00:00
abstract::Cerebellar granule neurons can be maintained in culture in a medium containing high serum and depolarising levels of KCl. When serum is removed and the KCl levels lowered from 25 to 5 mM, the cells undergo apoptosis. Apoptosis can be prevented by inhibitors of transcription or translation, suggesting a need for macrom...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)01687-1
更新日期:2001-04-20 00:00:00
abstract::LPS-induced microglial activation has a major influence on neuronal damage in the inflammatory process. Integral to this is the cellular and molecular interaction between microglia and neurons. Exosomes, a mediator of communication between cells, can transfer lipids, proteins and nucleic acids, affecting many donor an...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2019.134480
更新日期:2019-11-01 00:00:00
abstract::Human nerve growth factor (NGF) serum concentrations were measured in a healthy sample of 126 participants by a modified highly sensitive and specific two-site enzyme immunoassay. The measured NGF concentrations differ considerably from a normal distribution. The median NGF concentration was 19.68 pg/ml with an interq...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00403-8
更新日期:2003-06-19 00:00:00
abstract::It has been shown that orexin neuropeptides contribute to morphine-induced physical dependence. The locus coeruleus (LC), which receives a dense extra-hypothalamic orexinergic projection, is a key brain region implicated in the expression of somatic signs of morphine withdrawal syndrome. The aim of the present study i...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.07.050
更新日期:2010-10-04 00:00:00
abstract::The lysate of an immortalized monoclonal cell line derived from the striatum (X61) contains a dopaminergic stimulatory activity that is capable of increasing the dopamine content of an immortalized mouse mesencephalic cell line (MN9D) which expresses a dopaminergic phenotype. Purification of an isoamyl alcohol extract...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.11.021
更新日期:2005-03-07 00:00:00
abstract::Sleep fragmentation due to intermittent nocturnal arousal resulting in a reduction of total sleep time and sleep efficiency is a common symptom among people with Alzheimer's disease (AD) and elderly people with normal cognitive function. Although epidemiological studies have indicated an association between sleep frag...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2017.05.054
更新日期:2017-07-13 00:00:00
abstract::The effect of the N-methyl-D-aspartate (NMDA) receptor antagonist, 3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP), on the ethanol-evoked release of brain ascorbate (AA) was investigated in freely moving rats using an in vivo voltammetry technique. Ethanol (1.0 g/kg, intraperitoneal injection, i.p.) increase...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90655-5
更新日期:1993-07-09 00:00:00
abstract::The present study examined effects of high altitude exposure, which leads to development of hypoxia, on epiplexus cells associated with the choroid plexus in adult rats. At 2-5 h following exposure, epiplexus cells expressing inducible nitric oxide synthase (iNOS) were commonly observed. Increased expression of comple...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01070-3
更新日期:2000-05-19 00:00:00
abstract::Recently, research on lateralized event related potentials (ERPs) in response to irrelevant distractors has revealed that angry but not happy schematic distractors capture spatial attention. Whether this effect occurs in the context of the natural expression of emotions is unknown. To fill this gap, observers were ask...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2016.11.055
更新日期:2017-01-10 00:00:00
abstract::Beta,beta'-Iminodipropionitrile (IDPN) is known to produce a massive accumulation of neurofilaments in the proximal portion of axons of spinal anterior horn cells. The spinal cords of 20 Wistar rats treated with IDPN were examined immunohistochemically with a monoclonal antibody against heparan sulfate (HepSS-1). Virt...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)12225-7
更新日期:1995-12-29 00:00:00
abstract::To search for brain molecules that share epitopes with HIV (human immunodeficiency virus) proteins, we investigated non-AIDS human brains by immunohistochemical and Western blotting methods, and detected a 23 kDa protein which was immunoreactive with antibodies against HIVp17, a core protein of HIV with a molecular we...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90577-0
更新日期:1994-11-07 00:00:00
abstract::Spinal cord injury (SCI) results in rapid and significant oxidative stress. We have previously demonstrated that polyethylene glycol (PEG) repairs neuronal membrane and inhibits lipid peroxidation in an in vitro model of SCI. In this study we tested the effects of PEG on oxidative stress in guinea pigs after SCI. Oxid...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.02.027
更新日期:2004-04-15 00:00:00
abstract::Stroke is a major cause of epilepsy, but the molecular mechanisms underlying post-stroke epileptogenesis are unknown. The expression of cystatin C, a cysteine protease inhibitor, is increased in the hippocampus during status epilepticus (SE)-induced epileptogenesis, and regulates both cell death and birth. To test the...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.10.091
更新日期:2006-03-06 00:00:00
abstract::Calcium antagonists inhibit both the Ca2(+)-dependent and -independent release of endogenous glutamate from intact synaptosomes. In the present study, the inhibitory potency of several different classes of calcium antagonists were determined under conditions that control for an effect of these compounds on the Ca2(+)-...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90836-x
更新日期:1990-11-13 00:00:00
abstract::DJ-1, the causative gene of a familial form of Parkinson's disease (PD), has been reported undergo oxidation preferentially at the 106th cysteine residue (Cys-106) under oxidative stress. Recently, it has been found that the levels of oxidized DJ-1 in erythrocytes of unmedicated PD patients are markedly higher than th...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.08.007
更新日期:2010-10-15 00:00:00
abstract::The retinohypothalamic tract (RHT) in the Djungarian hamster Phodopus sungorus was studied using anterograde neuronal transport of cholera toxin subunit B (CTB) following unilateral intraocular injection. A major projection terminates bilaterally in the hypothalamic suprachiasmatic nuclei (SCN). In the anterior ventra...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90116-3
更新日期:1994-06-06 00:00:00
abstract::The representation of time, space and numbers are strictly linked in the primate's cognitive system. Here we show that merely looking at number symbols biases a temporal judgment on their duration depending upon the number's magnitude. In a first experiment, a group of healthy subjects was submitted to a time estimati...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.04.051
更新日期:2008-06-27 00:00:00
abstract::The effect of dopamine on the 3',5'-cyclic adenosine monophosphate (cAMP) generating system of membrane particles of rat prostate gland was studied. Dopamine increased the concentration of cAMP in a dose-dependent manner. The selective D1 receptor inhibitor SCH 23390 caused a decrease in dopamine-elicited cAMP levels....
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90608-2
更新日期:1987-06-01 00:00:00
abstract::Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels which mediate fast cholinergic synaptic transmission in insect and vertebrate nervous systems. A point mutation Y151S had been identified in Nilaparvata lugens (brown planthopper) that is associated with target-site resistance to neonicotinoid in...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.09.039
更新日期:2008-12-03 00:00:00
abstract::Oxytoxin (OT) promotes social behavior and reduces anxiety. A great number of studies suggest that OT plays a role in learning and memory processes in animals and humans. Fear memories are rendered labile and prone to modification after reactivation and a restabilization and reconsolidation process is necessary for fu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2015.03.030
更新日期:2015-05-06 00:00:00
abstract::Localized moderate cooling (+10 degrees C) of the anterior and ventral part of the raphe dorsalis induced cortical synchronization and ponto-geniculo-occipital (PGO) waves followed sometimes by paradoxical sleep. Cooling the region of the raphe magnus produced cortical arousal. After depletion of brain 5-hydroxytrypta...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(76)90077-x
更新日期:1976-11-01 00:00:00
abstract::Spinocerebellar ataxia type 2 (SCA2) is associated with an expansion of CAG/polyglutamine-repeat of a gene of unknown function. We performed an immunohistochemical study to identify the immunolocalization of the disease protein ataxin-2 in normal and SCA2 patients. Although normal and expanded ataxin-2 were ubiquitous...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(99)00656-4
更新日期:1999-10-01 00:00:00
abstract::Elongation factor-2 kinase (eEF-2K) is a Ca(2+)/calmodulin-dependent protein kinase that is highly specific for eukaryotic elongation factor-2. In the present study, we examined the developmental expression of eEF-2K messenger RNA (mRNA) in the mouse brain. At E13, the expression of eEF-2K mRNA is already evident in b...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(02)00715-2
更新日期:2002-09-13 00:00:00
abstract::Gap junction (GJ) coupling between neocortical GABAergic interneurons plays a critical role in the synchronization of activity in cortical networks in physiological and pathophysiological states, e.g., seizures. Past studies have shown that GJ blockers exert anticonvulsant actions in both in vivo and in vitro models o...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.01.042
更新日期:2007-04-18 00:00:00
abstract::In this study we reported evidence for the existence of specific binding sites for platelet-activating factor (PAF) in neuroblastoma N1E-115 cells. The specific [3H]PAF binding reached a steady state level within 60 min at 25 degrees C. Scatchard analysis of the specific [3H]PAF binding revealed the presence of two ap...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11319-r
更新日期:1995-02-17 00:00:00
abstract::The kynurenine pathway converts tryptophan into various compounds, including L-kynurenine, which in turn can be converted to the excitatory amino acid receptor antagonist kynurenic acid. The hypothesis that endogenously-produced kynurenic acid could have physiological effects was tested in combined entorhinal/hippocam...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)13472-3
更新日期:1997-03-14 00:00:00
abstract::Central dopaminergic systems have been implicated in the regulation of blood pressure. We examined the effect on blood pressure of electrical or chemical stimulation of the rat brain ventral tegmental area (VTA) which is the region of origin of the A10 dopaminergic system. Electrical stimulation in urethane-anaestheti...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90068-x
更新日期:1994-08-01 00:00:00
abstract::Rats with suprachiasmatic nuclei (SCN) lesions did not show increased sleep after triazolam (TRZ) injections at any dose from 0.2 to 1.6 mg/kg, whereas 0.4 mg/kg TRZ given intact rats in the middle of their activity phase significantly increased sleep. Across SCN-lesioned and intact rats, the amount of sleep before an...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(91)90007-g
更新日期:1991-04-29 00:00:00