DPP and DSP are Necessary for Maintaining TGF-β1 Activity in Dentin.

Abstract:

:Porcine dentin sialophosphoprotein (DSPP) is the most abundant non-collagenous protein in dentin. It is processed by proteases into 3 independent proteins: dentin sialoprotein (DSP), dentin glycoprotein (DGP), and dentin phosphoprotein (DPP). We fractionated DPP and DSP along with TGF-β activity by ion exchange (IE) chromatography from developing pig molars and measured their alkaline phosphatase (ALP)-stimulating activity in human periodontal (HPDL) cells with or without TGF-β receptor inhibitor. We then purified TGF-β-unbound or -bound DPP and DSP by reverse-phase high-performance liquid chromatography (RP-HPLC) using the ALP-HPDL system. The TGF-β isoform bound to DPP and DSP was identified as being TGF-β1 by both ELISA and LC-MS/MS analysis. We incubated carrier-free human recombinant TGF-β1 (CF-hTGF-β1) with TGF-β-unbound DPP or DSP and characterized the binding on IE-HPLC using the ALP-HPDL system. When only CF-hTGF-β1 was incubated, approximately 3.6% of the ALP-stimulating activity remained. DPP and DSP rescued the loss of TGF-β1 activity. Approximately 19% and 10% of the ALP stimulating activities were retained by the binding of TGF-β to DPP and DSP, respectively. The type I collagen infrequently bound to CF-hTGF-β1. We conclude that both DPP and DSP help retain TGF-β1 activity in porcine dentin.

journal_name

J Dent Res

authors

Yamakoshi Y,Kinoshita S,Izuhara L,Karakida T,Fukae M,Oida S

doi

10.1177/0022034514534690

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

671-7

issue

7

eissn

0022-0345

issn

1544-0591

pii

0022034514534690

journal_volume

93

pub_type

杂志文章
  • Longevity of posterior composite restorations: a systematic review and meta-analysis.

    abstract::The aim of this meta-analysis, based on individual participant data from several studies, was to investigate the influence of patient-, materials-, and tooth-related variables on the survival of posterior resin composite restorations. Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA...

    journal_title:Journal of dental research

    pub_type: 杂志文章,meta分析,评审

    doi:10.1177/0022034514544217

    authors: Opdam NJ,van de Sande FH,Bronkhorst E,Cenci MS,Bottenberg P,Pallesen U,Gaengler P,Lindberg A,Huysmans MC,van Dijken JW

    更新日期:2014-10-01 00:00:00

  • Dental fluorosis in an area of Kenya with 2 ppm fluoride in the drinking water.

    abstract::We examined 102 children born and reared in an area of rural Kenya with 2 ppm fluoride in the drinking water for dental fluorosis, using the index developed by Thylstrup and Fejerskov (1978). The prevalence of dental fluorosis was 100%, 92% of all teeth exhibited a TFI score of 4 or higher, and 50% of the children had...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345860650050501

    authors: Manji F,Baelum V,Fejerskov O

    更新日期:1986-05-01 00:00:00

  • Lasers for the treatment of dentin hypersensitivity: a meta-analysis.

    abstract::This systematic review and meta-analysis assessed the efficacy of lasers in reducing dentin hypersensitivity (DH) as compared with placebo or no treatment. Seven electronic databases and a manual search resulted in 2,538 unique publications. After selection, 13 studies were included in the meta-analysis. A CONSORT-bas...

    journal_title:Journal of dental research

    pub_type: 杂志文章,meta分析,评审

    doi:10.1177/0022034513487212

    authors: Sgolastra F,Petrucci A,Severino M,Gatto R,Monaco A

    更新日期:2013-06-01 00:00:00

  • Co-activation of sternocleidomastoid muscles during maximum clenching.

    abstract::In an attempt to determine the degree of co-activation present in selected cervical muscles during clenching, we instructed 12 male subjects to produce four brief maximum voluntary contraction (MVC) efforts (clenching) in a position of maximum intercuspation. Surface EMG activity was recorded bilaterally from the mass...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345930720110701

    authors: Clark GT,Browne PA,Nakano M,Yang Q

    更新日期:1993-11-01 00:00:00

  • Characterization of the alpha-amylase receptor of Streptococcus gordonii NCTC 7868.

    abstract::The purpose of the work described here was to investigate the mechanisms involved in the binding of salivary alpha-amylase to Streptococcus gordonii NCTC 7868 (Challis). Of six types of alpha-amylase studied, only mammalian forms of the enzyme were found to bind to S. gordonii cells. Salivary alpha-amylase binding was...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345900690110701

    authors: Douglas CW

    更新日期:1990-11-01 00:00:00

  • The sensitizing potential of periodontal dressings.

    abstract::The sensitizing potential of three periodontal dressing materials was evaluated by the guinea pig maximization test. The results indicated a difference in the sensitization rate of the three materials. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345780570110801

    authors: Haugen E,Hensten-Pettersen A

    更新日期:1978-11-01 00:00:00

  • Abnormally high levels of cystatin S in submandibular glands, saliva, and gingiva of plaque-resistant rats.

    abstract::To identify salivary biomarkers of periodontal diseases, we used plaque-resistant and -susceptible rats as animal models. The levels of salivary cystatin S in saliva, salivary glands, and gingiva were tested in Nembutal-anesthetized young and adult plaque-resistant and -susceptible rats of both sexes with and without ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345980770110801

    authors: Abe K,Okina A,Yano T,Gao C,Ohmori H,Ishibashi K,Nishiura T,Letić-Gavrilović A

    更新日期:1998-11-01 00:00:00

  • Chlorhexidine preserves dentin bond in vitro.

    abstract::Loss of hybrid layer integrity compromises resin-dentin bond stability. Matrix metalloproteinases (MMPs) may be partially responsible for hybrid layer degradation. Since chlorhexidine inhibits MMPs, we hypothesized that chlorhexidine would decelerate the loss of resin-dentin bonds. Class I preparations in extracted th...

    journal_title:Journal of dental research

    pub_type: 杂志文章,随机对照试验

    doi:10.1177/154405910708600115

    authors: Carrilho MR,Carvalho RM,de Goes MF,di Hipólito V,Geraldeli S,Tay FR,Pashley DH,Tjäderhane L

    更新日期:2007-01-01 00:00:00

  • Experimental marginal leakage around dental amalgams placed in artificial cavities.

    abstract::Experimental marginal leakage of high- and low-Cu amalgam restorations placed in acrylic teeth, where the cavities were untreated or painted with denatured collagen and/or a CaP slurry, was assessed after specimens were stored in a 1% NaCl solution for ten days and for one yr. All specimens stored for ten days showed ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630081801

    authors: Jodaikin A,Grossman ES

    更新日期:1984-08-01 00:00:00

  • Genome-wide Identification of Foxf2 Target Genes in Palate Development.

    abstract::Cleft palate is among the most common structural birth defects in humans. Previous studies have shown that mutations in FOXF2 are associated with cleft palate in humans and mice and that Foxf2 acts in a Shh-Foxf-Fgf18-Shh molecular network controlling palatal shelf growth. In this study, we combined RNA-seq and ChIP-s...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034520904018

    authors: Xu J,Liu H,Lan Y,Park JS,Jiang R

    更新日期:2020-04-01 00:00:00

  • Mechanical work during stress-field translation in the human TMJ.

    abstract::The pathomechanics of degenerative joint disease of the temporomandibular joint (TMJ) may involve fatigue produced by mechanical work on the articulating tissues. This study tested the hypotheses that mechanical work in the TMJ (i) varies with the type of mandibular activity, and (ii) is evenly distributed over TMJ su...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910608501106

    authors: Gallo LM,Chiaravalloti G,Iwasaki LR,Nickel JC,Palla S

    更新日期:2006-11-01 00:00:00

  • Inorganic polyphosphate induces osteoblastic differentiation.

    abstract::Inorganic polyphosphate [Poly(P)] is especially prevalent in osteoblasts. We tested the hypothesis that Poly(P) stimulates osteoblastic differentiation and polyphosphate metabolism for bone formation. The osteoblast-like cell line, MC 3T3-E1, was cultured with Poly(P), and gene expression was evaluated by real-time re...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034510363096

    authors: Usui Y,Uematsu T,Uchihashi T,Takahashi M,Takahashi M,Ishizuka M,Doto R,Tanaka H,Komazaki Y,Osawa M,Yamada K,Yamaoka M,Furusawa K

    更新日期:2010-05-01 00:00:00

  • Particle size distribution of food boluses after mastication of six natural foods.

    abstract::There is a large variability between and among individuals in the physiology of mastication, but it is not known whether this produces a similar variability in the particle sizes of food boluses at the end of the chewing process. Food boluses obtained just before swallowing were analyzed in ten subjects (aged 36.7 +/-...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章

    doi:10.1177/154405910408300713

    authors: Peyron MA,Mishellany A,Woda A

    更新日期:2004-07-01 00:00:00

  • Dual regulation of osteoclast differentiation by periodontal ligament cells through RANKL stimulation and OPG inhibition.

    abstract::Periodontal ligament (PDL) cells play an important role in maintaining the homeostasis of periodontal tissues. However, it is not known how PDL cells contribute to osteoclastogenesis. In this study, we examined the consequences of cell-to-cell interactions between peripheral blood mononuclear cells (PBMCs) and PDL cel...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345010800030801

    authors: Kanzaki H,Chiba M,Shimizu Y,Mitani H

    更新日期:2001-03-01 00:00:00

  • Prosthetic Abutment Height is a Key Factor in Peri-implant Marginal Bone Loss.

    abstract::In this study, we analyzed the influence of prosthetic abutment height on marginal bone loss (MBL) around implants in the posterior maxilla. In this retrospective cohort study, the radiographically determined MBL was related to the height of the abutments of internal conical connection implants at 6 and 18 months post...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034513519800

    authors: Galindo-Moreno P,León-Cano A,Ortega-Oller I,Monje A,Suárez F,ÓValle F,Spinato S,Catena A

    更新日期:2014-07-01 00:00:00

  • Proteolysis by MMP20 Prevents Aberrant Mineralization in Secretory Enamel.

    abstract::The present study was conducted to investigate the role of proteolysis by matrix metalloproteinase 20 (MMP20) in regulating the initial formation of the enamel mineral structure during the secretory stage of amelogenesis, utilizing Mmp20-null mice that lack this essential protease. Ultrathin sagittal sections of maxil...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034518823537

    authors: Yamazaki H,Tran B,Beniash E,Kwak SY,Margolis HC

    更新日期:2019-04-01 00:00:00

  • Cholinoreceptor autoantibodies in Sjögren syndrome.

    abstract::Previous studies have demonstrated that antibodies against cholinoreceptors of exocrine glands correlate with dry mouth in persons with primary Sjögren syndrome (pSS). The aim of the present investigation was to establish if serum IgG antibodies (pSS IgG) were able to interact with cholinoreceptors in rat submandibula...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910708600905

    authors: Reina S,Orman B,Anaya JM,Sterin-Borda L,Borda E

    更新日期:2007-09-01 00:00:00

  • The effects of an incisal bite plane on rat sublingual glands.

    abstract::Application of an incisal bite plane for as little as three d caused a significant increase in the size of the sublingual glands of rats, with some evidence of hyperplasia and hypertrophy of the acinar cells. The enlargement had few effects on salivary secretion, and the gland changes were reversible. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345830620060501

    authors: Hioki S,Niwa K,Kishimoto T,Kawazoe T,Yokota Y,Abe K,Dawes C

    更新日期:1983-06-01 00:00:00

  • In vivo remineralization using a sustained topical fluoride delivery system.

    abstract::The efficacy of a new remineralization system was determined in vivo by maintaining a low concentration of approximately 1 ppm fluoride for 48 hrs against a demineralized human tooth. Human subjects were selected who wore removable partial dentures containing two or more of the demineralized teeth with film system. Th...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345800590030501

    authors: Abrahams LJ,Yonese M,Higuchi WI,Fox JL,Charbeneau GT

    更新日期:1980-03-01 00:00:00

  • Kinetics of bone-resorbing activity in developing periapical lesions.

    abstract::The presence and kinetics of bone-resorbing activity in periapical lesions were studied with a rat model system. Lesions were found to expand most rapidly between induction on day 0 and day 15 ("active phase"), with enlargement occurring at a slower rate thereafter (days 20 and 30, "chronic phase"), as assessed by mea...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345910700100901

    authors: Wang CY,Stashenko P

    更新日期:1991-10-01 00:00:00

  • Site-specific expression of collagen I and XII mRNAs in the rat periodontal ligament at two developmental stages.

    abstract::In mammals, the periodontal ligament (PDL) is a highly specialized tissue which facilitates tooth eruption and lends mechanical support to the tooth once in occlusion. The PDL extracellular matrix fibers play a major role in such functions. During its development, the spatial arrangement of the PDL extracellular matri...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345920710070201

    authors: Karimbux NY,Rosenblum ND,Nishimura I

    更新日期:1992-07-01 00:00:00

  • Mouse cellular cementum is highly dependent on growth hormone status.

    abstract::Cementum is known to be growth-hormone (GH)-responsive, but to what extent is unclear. This study examines the effects of extremes of GH status on cementogenesis in three lines of genetically modified mice; GH excess (giant), GH antagonist excess (dwarf), and GH receptor-deleted (GHR-KO) (dwarf). Age-matched mandibula...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910408300107

    authors: Smid JR,Rowland JE,Young WG,Daley TJ,Coschigano KT,Kopchick JJ,Waters MJ

    更新日期:2004-01-01 00:00:00

  • Metabolism of gingival collagen in a rat.

    abstract::The remodeling of gingival tissue was measured by determining the incorporation of tritiated proline and the disappearance of tritium activity from proline and hydroxyproline for 18 days after administration of label. There was a rapid decrease in the tritium activity from gingiva which was paralleled by a decrease in...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345780570023101

    authors: Orlowski WA

    更新日期:1978-02-01 00:00:00

  • Short-chain carboxylic acid concentration in human gingival crevicular fluid.

    abstract::Short-chain carboxylic acids (e.g., lactic acid, propionic acid, butyric acid) are metabolic by-products of bacterial metabolism which can accumulate in the gingival crevice. It is of no small consequence, therefore, that 1- to 5-mM concentrations of these acids exhibit significant biological activity, including the a...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345970760010801

    authors: Niederman R,Buyle-Bodin Y,Lu BY,Robinson P,Naleway C

    更新日期:1997-01-01 00:00:00

  • Standardization and safety of alveolar bone-derived stem cell isolation.

    abstract::Cell therapies utilizing mesenchymal stem cells (MSCs) could overcome limitations of traditional treatments for reconstructing craniofacial tissues. This large-scale study explored a standardized methodology for the isolation and clinical-scale expansion of alveolar bone marrow-derived MSCs (aBMSCs). We harvested 103 ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034513510530

    authors: Mason S,Tarle SA,Osibin W,Kinfu Y,Kaigler D

    更新日期:2014-01-01 00:00:00

  • Artificial Intelligence in Dentistry: Chances and Challenges.

    abstract::The term "artificial intelligence" (AI) refers to the idea of machines being capable of performing human tasks. A subdomain of AI is machine learning (ML), which "learns" intrinsic statistical patterns in data to eventually cast predictions on unseen data. Deep learning is a ML technique using multi-layer mathematical...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034520915714

    authors: Schwendicke F,Samek W,Krois J

    更新日期:2020-07-01 00:00:00

  • Apoptosis in human oral squamous cell carcinomas is induced by 15-deoxy-delta 12,14-prostaglandin J2 but not by troglitazone.

    abstract::15-deoxy-Delta(12,14)-prostaglandin J(2) (15-d-PGJ(2)) and troglitazone have been shown to induce apoptosis in several carcinoma cell lines. However, apoptotic signaling pathways of these agents are poorly understood. We tested the hypothesis that peroxisome proliferator-activated receptor-gamma ligands such as these ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910308201008

    authors: Fukuchi K,Date M,Azuma Y,Shinohara M,Takahashi H,Ohura K

    更新日期:2003-10-01 00:00:00

  • Mandibulofacial adaptations in a juvenile animal model of temporomandibular joint arthritis.

    abstract::Juvenile rheumatoid arthritis (JRA) is a chronic systemic disease of childhood that affects synovial joints including the temporomandibular joint (TMJ). Individuals with JRA of the TMJ frequently show aberrations in mandibulofacial development. Since the basis for these developmental perturbations is poorly understood...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345990780080801

    authors: Tavakkoli-Jou M,Miller AJ,Kapila S

    更新日期:1999-08-01 00:00:00

  • Genetic association of ARHGAP21 gene variant with mandibular prognathism.

    abstract::Mandibular prognathism (MP) is a recognizable phenotype associated with dentoskeletal class III malocclusion. MP is a complex genetic trait, although familial recurrence also suggests the contribution of single inherited variations. To date, the genetic causes of MP have been investigated using linkage analysis or ass...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034515572190

    authors: Perillo L,Monsurrò A,Bonci E,Torella A,Mutarelli M,Nigro V

    更新日期:2015-04-01 00:00:00

  • Prevalence and need for replacement of amalgam and composite restorations in Dutch adults.

    abstract::In 1986, the first nationwide epidemiological survey of adult oral health and attitudes to oral health care was carried out in The Netherlands. One of the aims of this study was to evaluate the prevalence and the quality (in terms of need for replacement) of amalgam and composite restorations in the Dutch adult popula...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345900690060901

    authors: Kroeze HJ,Plasschaert AJ,van 't Hof MA,Truin GJ

    更新日期:1990-06-01 00:00:00