Abstract:
:Inorganic polyphosphate [Poly(P)] is especially prevalent in osteoblasts. We tested the hypothesis that Poly(P) stimulates osteoblastic differentiation and polyphosphate metabolism for bone formation. The osteoblast-like cell line, MC 3T3-E1, was cultured with Poly(P), and gene expression was evaluated by real-time reverse-transcription polymerase chain-reaction. Phosphatase activity and extracellular matrix mineralization were also determined. The role of Poly(P) was assessed in a beagle dog alveolar bone regeneration model. Poly(P) increased osteocalcin, osterix, bone sialoprotein, and tissue non-specific alkaline phosphatase gene expression, with a high level of end-polyphosphatase activity, resulting in low-chain-length Poly(P), inorganic pyrophosphate, and inorganic phosphate production. MC3T3-E1 cells differentiated into mature osteoblasts and showed expression of ectonucleotide pyrophosphatase phosphodiesterase 1, while mouse progressive ankylosis gene expression remained unchanged. Promotion of alveolar bone regeneration was observed in Poly(P)-treated beagle dogs. These findings suggest that Poly(P) induces osteoblastic differentiation and bone mineralization, and acts as a resource for mineralization.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Usui Y,Uematsu T,Uchihashi T,Takahashi M,Takahashi M,Ishizuka M,Doto R,Tanaka H,Komazaki Y,Osawa M,Yamada K,Yamaoka M,Furusawa Kdoi
10.1177/0022034510363096subject
Has Abstractpub_date
2010-05-01 00:00:00pages
504-9issue
5eissn
0022-0345issn
1544-0591pii
0022034510363096journal_volume
89pub_type
杂志文章abstract::Sensitivity or pain of the mucoperiosteum covering the mandibular edentulous ridge is often thought to limit bite forces in complete-denture wearers. Therefore, bite forces with mandibular implant-retained overdentures may depend on the degree of implant support. This study analyzed the effects of different degrees of...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1177/00220345980770101101
更新日期:1998-10-01 00:00:00
abstract::The pathophysiology of painful temporomandibular disorders is not fully understood, but evidence suggests that muscle pain modulates motor function in characteristic ways. This study tested the hypothesis that activation of nociceptive muscle afferent fibers would be linked to an increased excitability of the human ja...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910208100915
更新日期:2002-09-01 00:00:00
abstract::Data from the actual private practices of two dentists were compared with respective data from the participation of the same two dentists in a laboratory model of private practice conducted in the University of North Carolina Dental Research Center. The findings show that a laboratory model can be established which ac...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345750540032501
更新日期:1975-05-01 00:00:00
abstract::Although it is known that diabetes impairs oral wound healing, relatively little is known about the cellular parameters affected, particularly in connective tissue. This study investigated the hypothesis that diabetes impairs connective tissue formation in healing gingiva, and that impaired healing is associated with ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034510362960
更新日期:2010-06-01 00:00:00
abstract::Continuous etching of aggressive all-in-one adhesives occurs in wet dentin tubules after polymerization of the adhesives. This study challenged the hypothesis that unpolymerized acidic monomers from an aggressive all-in-one self-etching adhesive continue to etch beyond dentin hybrid layers. Dentin surfaces bonded with...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034510363665
更新日期:2010-05-01 00:00:00
abstract::The dimensional accuracy of porcelain fused to metal crown and bridge castings was determined on truncated cone-shaped steel dies. Ni-Cr castings produced in manufacturers' laboratories were consistently undersize, while precious metal castings were consistently oversize. Ni-Cr castings, produced in NBS laboratories u...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345790580120501
更新日期:1979-12-01 00:00:00
abstract::The effect of different doses of polyadenylic and polyuridylic acids (poly A:U) was studied in control rats and in rats exposed to hypoxia. In the control rats, administration of different doses of poly A:U did not change the thickness of the epiphyseal plate or increase the incorporation of 3H-phenylalanine as judged...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345760550041701
更新日期:1976-07-01 00:00:00
abstract::The human temporalis and masseter muscles are not activated homogeneously during static bite force tasks. In this study, we studied the possible existence of regional differences in these muscles under dynamic conditions. Electromyographic (EMG) activity was recorded by means of bipolar fine-wire electrodes. Six elect...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345950740061201
更新日期:1995-06-01 00:00:00
abstract::Moderate elevation of serum C-reactive protein (CRP) is a risk factor for cardiovascular disease among apparently healthy individuals, although factors that create this inflammatory response in the absence of systemic illness have not been clarified. This study aimed to: (1) evaluate associations among periodontal dis...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345000790010701
更新日期:2000-01-01 00:00:00
abstract::The oral microbiome is one of the most stable ecosystems in the body and yet the reasons for this are still unclear. As well as being stable, it is also highly diverse which can be ascribed to the variety of niches available in the mouth. Previous studies have focused on the microflora in disease-either caries or peri...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034520915486
更新日期:2020-06-01 00:00:00
abstract::Interfacial bond strengths of light-activated composites were measured as a function of age using a transverse strength test. Bond strength between layers decreased with the age of the initial layer and reflected the setting curves of the composites. The highly-filled composites exhibited the greatest bond strengths. ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630101501
更新日期:1984-10-01 00:00:00
abstract::Minimally invasive caries-removal procedures remove only caries-infected dentin and preserve caries-affected dentin that becomes remineralized. Dental cements containing calcium phosphate promote remineralization. This study evaluated the in vivo remineralization capacity of resin-based calcium-phosphate cement (Ca-P)...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034509360155
更新日期:2010-03-01 00:00:00
abstract::Six subjects wore intra-oral devices carrying eight bovine enamel blocks which were covered with a layer of S. mutans. They rinsed their mouths for five sec, one min, or multiples of one min with a 5 or 10% glucose (G) solution. Demineralization was measured after 45 min by determining the change in iodide permeabilit...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345860650021001
更新日期:1986-02-01 00:00:00
abstract::Selected strains of oral bacteria were analyzed for their ability to degrade wheat starch, maltose, maltotriose, and maltoheptaose. S. sanguis IUOM-11M and JC804, S. mutans 6715, S. salivarius IUOM-8, A. viscosus IUOM-62, and A. naeslundii ATCC 12104 degraded all four substrates. S. mutans NCTC 10449 degraded starch, ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345880670011501
更新日期:1988-01-01 00:00:00
abstract::Damage to the dentin matrix investigates the proliferation and mobilization of dental progenitor cells to the injury site, the mechanisms of which are not defined. EphB receptors and ephrin-B ligands expressed within the perivascular niche of dental pulp have been implicated following tooth injury. We propose that ele...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034509342363
更新日期:2009-09-01 00:00:00
abstract::We previously demonstrated that an anti-caries DNA vaccine intranasally administered with recombinant flagellin protein as a mucosal adjuvant enhanced salivary IgA response and conferred better protection against caries. However, the relatively weak immunogenicity of DNA vaccines and the necessity for a large quantity...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034512457684
更新日期:2012-10-01 00:00:00
abstract::Mark Twain provides a humorous and insightful look into the origins of periodontal therapy and anesthesia in "Happy Memories of the Dental Chair". The main character of this story is Dr. John Riggs, the father of periodontics. Dr. Horace Wells, a pioneer in the field of anesthesia, also appears in the story. Twain pre...
journal_title:Journal of dental research
pub_type: 传,历史文章,杂志文章
doi:10.1177/0022034510366677
更新日期:2010-07-01 00:00:00
abstract::Periodontitis is characterized by periodontal tissue destruction. Since interleukin-17 (IL-17) has been reported to up-regulate IL-1beta and tumor necrosis factor-alpha (TNF-alpha), it was hypothesized that it is increased in periodontitis and up-regulates these cytokines and tissue-destructive matrix metalloproteinas...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910708600409
更新日期:2007-04-01 00:00:00
abstract::Improved mechanical properties of resin composites have been reported to be a consequence of the addition of aldehyde to the resins. The objective of the present study was to examine whether this improvement could be attributed to an increased degree of conversion of double bonds. For this purpose, propanal was added ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345940730101101
更新日期:1994-10-01 00:00:00
abstract::The effects of Escherichia coli endotoxin upon mouse L929 cell proliferation, DNA synthesis, protein synthesis, and proline incorporation were determined. It was found that a level of endotoxin which inhibited cell proliferation prompted a similar inhibition of DNA synthesis and overall cell protein synthesis. In cont...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345790580061301
更新日期:1979-06-01 00:00:00
abstract::The aim of this study was to investigate the involvement of autoimmune reactions to native and post-translationally modified extracellular matrix components in the pathogenesis of periodontitis. Sera from individuals with aggressive periodontitis (AgP, n = 25), chronic periodontitis (CP, n = 14), and gingivitis (G, n ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034510381903
更新日期:2010-12-01 00:00:00
abstract::Clinical studies have shown that metabolic syndrome (MetS) is associated with increased risk of developing periodontitis. However, the underlying mechanisms remain largely unknown. Since it is known that lipopolysaccharide (LPS)-activated toll-like receptor 4 signaling pathways play a crucial role in periodontitis, we...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034514561658
更新日期:2015-02-01 00:00:00
abstract::Significant associations between atherosclerosis and both Porphyromonas gingivalis, a major periodontopathogen, and the respiratory pathogen, Chlamydia pneumoniae, have been shown. Many individuals with evidence of atherosclerosis demonstrate seropositivity to these pathogens. The aim of the present study was to exami...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910708600105
更新日期:2007-01-01 00:00:00
abstract::The effect of low-level laser treatment on maxillary arch dimensions after palatal surgery was investigated in Beagle dogs at an age of 12 weeks. A total of 30 dogs was used, and they were assigned to either a control group (Group C, n = 6) or one of two experimental groups (Group L, n = 12; and group LL, n = 12). Aft...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345910700111501
更新日期:1991-11-01 00:00:00
abstract::Previous studies showed that residual compressive stresses induced by thermal tempering retarded the growth of surface cracks in bilayered porcelain disks. The objectives of the present study were: (1) to determine whether thermal tempering by air blasting reduces the length of cracks induced by microhardness indentat...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345960750020301
更新日期:1996-02-01 00:00:00
abstract::Investigations of the maturation pattern of rat incisor enamel by quantitative electron microscopy and electron-probe microanalysis indicate that mineralization of rat enamel can be regarded, as in humans, as a regular and progressive process pattern of enamel matrix formation. The species variations that have been pr...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345790580024201
更新日期:1979-03-01 00:00:00
abstract::The flexure moduli of elasticity of solid and multi-stranded orthodontic wires were determined. Results for the solid wires approximated their tensile values, but the differences were statistically significant for the stainless steel, chromium-cobalt-nickel, and nickel-titanium alloys. The flexure moduli of the multi-...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620072201
更新日期:1983-07-01 00:00:00
abstract::Using a hydraulic bellows system mounted on a maxillary partial denture, continuous force-displacement data were taken in an edentulous area in the maxillary bicuspid-molar region on ten adults. Both loading and unloading force-displacement curves showed three segments. Two visco-elastic properties - hysteresis and re...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630101901
更新日期:1984-10-01 00:00:00
abstract::Although dental amalgam is used frequently under artificial crowns for restoration of severely damaged teeth, there is little information available on the bond between luting cements and this alloy. This study was designed for determination of the strength of the bond between a dental amalgam alloy and three crown-lut...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345890680111501
更新日期:1989-11-01 00:00:00
abstract::Carbamide peroxide (CP) is widely used as a tooth-whitening agent in self-administered tooth-bleaching products. In this study, the effects of 5% and 10% CP on dentinal collagen structure and chemical properties were evaluated in vitro. Thirty-five intact teeth were exposed to 2 whitening protocols (2 or 4 h daily) wi...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034518822826
更新日期:2019-04-01 00:00:00