Abstract：
:To identify salivary biomarkers of periodontal diseases, we used plaque-resistant and -susceptible rats as animal models. The levels of salivary cystatin S in saliva, salivary glands, and gingiva were tested in Nembutal-anesthetized young and adult plaque-resistant and -susceptible rats of both sexes with and without chronic treatment with isoproterenol. Isoproterenol was injected i.p. once a day for 4 or 6 consecutive days. Isoelectric focusing electrophoresis by the PhastSystem and the western blotting method were used to separate different proteins and to identify a salivary cystatin S band in these samples. The expression of salivary cystatin S mRNA was also determined by the northern blotting method. Depending upon the types of agonists, a few differences were observed in secretory functions between both strains of rats in both sexes, but the levels of salivary cystatin S in saliva elicited from the submandibular gland and in the extracts of the submandibular glands and gingiva were significantly higher in plaque-resistant rats when compared with those of plaque-susceptible rats in both sexes. However, no significant difference was seen between the strains after chronic treatment with isoproterenol. The N-terminal 26-amino-acid sequence of salivary cystatin S purified from submandibular saliva of plaque-resistant rats was identical with that purified from submandibular saliva of Sprague-Dawley rats subjected to chronic treatment with isoproterenol. The expression of salivary cystatin S mRNA was dramatic in the submandibular glands of the plaque-resistant rats and in the submandibular glands of Wistar rats subjected to chronic treatment with isoproterenol, but not in those of plaque-susceptible rats. These results suggest that salivary cystatin S might be a good biomarker in distinguishing between the two strains of rats and that its concentration is correlated with plaque resistance.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Abe K,Okina A,Yano T,Gao C,Ohmori H,Ishibashi K,Nishiura T,Letić-Gavrilović Adoi
10.1177/00220345980770110801subject
Has Abstractpub_date
1998-11-01 00:00:00pages
1913-9issue
11eissn
0022-0345issn
1544-0591journal_volume
77pub_type
杂志文章abstract：:Components of resin materials may damage DNA, leading to genetic alterations in mammalian cells. Here, monomers were analyzed for the induction of chromosomal aberrations indicated by micronuclei induced in V79 cells. A dose-related increase in the numbers of micronuclei was observed with triethyleneglycol dimethacryl...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345010800070401
更新日期：2001-07-01 00:00:00
abstract：:After cheek pouch carcinomas were induced in hamsters by the application of dimethylbenzanthracene (DMBA) to the right pouch for 13 weeks, the animals were divided into four groups and observed for seven more weeks. The control group received no further treatment, two experimental groups had incisional biopsies perfor...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650061401
更新日期：1986-06-01 00:00:00
abstract：:In 1992 and 1997, all US and Canadian dental schools were surveyed by mail regarding the preferred initial radiographic examination prescribed for non-emergency, comprehensive-care patients (dentulous adults, edentulous adults, and children). In both survey years, a minority of US and Canadian dental schools reported ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345000790071701
更新日期：2000-07-01 00:00:00
abstract：:Debonding rates of 164 resin-bonded prostheses (RBP) placed from 1974 to 1985 were examined at recall. The yearly debond rates were calculated, the cumulative probability of failure was determined for the debonded RBPs, and Weibull distribution parameters were determined. For perforated prostheses, there was a high in...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345930720081401
更新日期：1993-08-01 00:00:00
abstract：:Matrix metalloproteinases (MMPs) produced by resident and inflammatory cells in response to periodontopathogens play a major role in periodontal tissue destruction. Our aim was to investigate the effects of A-type cranberry proanthocyanidins (AC-PACs) on: (i) the production of various MMPs by human monocyte-derived ma...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034509339487
更新日期：2009-07-01 00:00:00
abstract：:The cacao bean husk has been shown to possess two types of cariostatic substances, one showing anti-glucosyltransferase (GTF) activity and the other antibacterial activity, and to inhibit experimental dental caries in rats infected with mutans streptococci. In the present study, chromatographic purification revealed h...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345010800111001
更新日期：2001-11-01 00:00:00
abstract：:1) A new adhesive opaque resin containing a reactive monomer, 4-methacryloxy-ethyl trimellitate anhydride (4-META), was prepared, and its application to thermosetting acrylic resin veneer crowns was studied. 2) The 4-META opaque resin was applied to a variety of nickel-chromium dental alloy specimens which had undergo...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600091101
更新日期：1981-09-01 00:00:00
abstract：:The benefits of early orthodontic treatment are continuously discussed, but studies are few. We examined whether definite need for orthodontic treatment could be eliminated in public health care by systematically focusing on early intervention. One age cohort living in a rural Finnish municipality (N = 85) was regular...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910808700604
更新日期：2008-06-01 00:00:00
abstract：:The aim of this study was to determine whether the parotid gland, like other salivary glands, produces blood group antigens. A post-embedding immunogold staining (IGS) method for ABH antigens was applied to osmicated and nonosmicated, routinely prepared specimens of normal human parotid glands. A and B antigens were n...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345940730021101
更新日期：1994-02-01 00:00:00
abstract：:In the past two decades, accumulated evidence has clearly demonstrated the inhibitory effects of laser irradiation on enamel demineralization, but the exact mechanisms of these effects remain unclear. The purpose of this study was to investigate the effects of low-energy CO2 laser irradiation on demineralization of bo...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345000790091401
更新日期：2000-09-01 00:00:00
abstract：:Minimal invasive approaches to managing caries, such as partial caries removal techniques, are showing increasing evidence of improved outcomes over the conventional complete caries removal. There is also increasing interest in techniques where no caries is removed. We present the 1-yr results of clinical efficacy for...
journal_title：Journal of dental research
pub_type： 杂志文章,随机对照试验
doi：10.1177/0022034514550717
更新日期：2014-11-01 00:00:00
abstract：:During orofacial tissue development, the anterior and posterior regions of the Meckel's cartilage undergo mineralization, while the middle region undergoes degeneration. Despite the interesting and particular phenomena, the mechanisms that regulate the different fates of Meckel's cartilage, including the effects of bi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034520960118
更新日期：2021-02-01 00:00:00
abstract：:The human mandibular condyle has a parasagittal plate-like trabecular structure. We tested the hypothesis that this structure reflects the mechanical loading of the condyle. We developed a finite element model of the condyle to analyze the strains occurring during static compressive loading. The principal strains in t...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910208101010
更新日期：2002-10-01 00:00:00
abstract：:Multiple interactions occur among major determinants of dental caries. We have studied the bacterial flora and pH-lowering capacity of the same dental plaques in relation to caries. The findings on the plaque flora are reported here. The buccal surfaces of upper teeth in each subject were selected for study. A low-car...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345000790021201
更新日期：2000-02-01 00:00:00
abstract：:The fatigue crack growth resistance of dentin was characterized as a function of depth from the dentino-enamel junction. Compact tension (CT) specimens were prepared from the crowns of third molars in the deep, middle, and peripheral dentin. The microstructure was quantified in terms of the average tubule dimensions a...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034511408429
更新日期：2011-08-01 00:00:00
abstract：:Adhesion of epithelium to the extracellular matrix is crucial for the maintenance of systemic and oral health. In the oral cavity, teeth or artificial dental implants penetrate the soft tissue of the gingiva. In this interface, gingival soft tissue needs to be well attached via the epithelial seal to the tooth or impl...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034511402207
更新日期：2011-12-01 00:00:00
abstract：:Non-steroidal anti-inflammatory drugs (NSAIDs) are used to manage pain and inflammatory disorders. We hypothesized that gingival fibroblasts actively accumulate NSAIDs and enhance their levels in gingival connective tissue. Using fluorescence to monitor NSAID transport, we demonstrated that cultured gingival fibroblas...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910608500511
更新日期：2006-05-01 00:00:00
abstract：:Known antimalarial compunds from several chemical classes were tested for their ability to inhibit in vitro growth of microorganisms causing dental plaque and to prevent the development of plaque in a hamster model. Approximately a third of the compounds tested inhibited in vitro growth; however, none prevented develo...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345750540030901
更新日期：1975-05-01 00:00:00
abstract：:Defined sets of transcriptional factors can reprogram human somatic cells to induced pluripotent stem (iPS) cells. However, many types of human cells are not easily accessible to minimally invasive procedures. Here we evaluated dental pulp cells (DPCs) as an optimal source of iPS cells, since they are easily obtained ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034510366846
更新日期：2010-08-01 00:00:00
abstract：:The uptake patterns of 90Sr in developing rat molar enamel were studied in vivo and in vitro. Autoradiographic methods were used that preclude loss or translocation of tracers associated with water-soluble compounds in the sections. In eight-day-old rats injected with the tracer, 90Sr uptake in the enamel was signific...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345800590121301
更新日期：1980-12-01 00:00:00
abstract：:In mammals, the periodontal ligament (PDL) is a highly specialized tissue which facilitates tooth eruption and lends mechanical support to the tooth once in occlusion. The PDL extracellular matrix fibers play a major role in such functions. During its development, the spatial arrangement of the PDL extracellular matri...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345920710070201
更新日期：1992-07-01 00:00:00
abstract：:Interfacial bonding and stability of gamma-methacryloxypropyltrimethoxysilane with silica surfaces have been studied by means of infrared spectroscopy. The addition of n-propylamine enhances silanization of gamma-methacryloxypropyltrimethoxysilane to silica surfaces in normal aliphatic hydrocarbons, and cyclohexane yi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345820610121301
更新日期：1982-12-01 00:00:00
abstract：:Mixed or parotid saliva from caries-active individuals consistently supported better growth of Streptococcus mutans (type c) than that from caries-free individuals. Electrophoretic studies revealed that certain proteins in caries-active salivas were susceptible to microbial attack, but similar proteins in caries-free ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580101501
更新日期：1979-10-01 00:00:00
abstract：:The sequelae of sustained, in vivo delivery of two important neurotransmitter substances, glutamate and thyrotropin-releasing hormone (TRH), upon craniofacial growth and development have previously not been investigated. Our purpose was to document and compare the relative effects of glutamate and TRH microspheres ste...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345970760080601
更新日期：1997-08-01 00:00:00
abstract：:Several studies have shown that surgical detachment of marginal gingiva close to the cervical cementum of molar teeth in a rat mandible is a distinct stimulus for alveolar bone resorption. Recently, we found that P2X4, an ATP-receptor, is significantly up-regulated in marginal gingival cells soon after surgery. We hyp...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910708600214
更新日期：2007-02-01 00:00:00
abstract：:In comparison with control rats. chronic administration of isoproterenol (IPR) caused enlargement of the parotid and submandibular glands and the synthesis of apparently new proteins, probably by acinar cells. The parotid and submandibular saliva secreted in response to methoxamine or IPR differed markedly in protein ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345800590062001
更新日期：1980-06-01 00:00:00
abstract：:The human patched gene (PTCH) functions in both embryologic development and tumor suppression. PTCH mutations have been found in odontogenic keratocysts. However, the expression and localization of the protein product of the gene have not been determined in odontogenic tumors and cysts. We investigated 68 odontogenic ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0810757
更新日期：2002-11-01 00:00:00
abstract：:Previously, we reported that the rate (R) of hydroxyapatite dissolution in acetic, lactic, and phosphoric acid solutions is a function of the degree of saturation with respect to the dissolving mineral, DS (defined as the ratio of the mean ionic activity product for hydroxyapatite [Ca5OH(PO4)3] in solution to its solu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345990780070701
更新日期：1999-07-01 00:00:00
abstract：:The synchronized cyrstal dissolution hypothesis previously proposed to explain the unusual dissolution behavior of human dental enamel and hydroxyapatite in partially saturated acidic media has been critically examined with dissolution-dialysis transport experiments. The findings are in accord with the hypothesis. A m...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560040701
更新日期：1977-04-01 00:00:00
abstract：:In 1986, the first nationwide epidemiological survey of adult oral health and attitudes to oral health care was carried out in The Netherlands. One of the aims of this study was to evaluate the prevalence and the quality (in terms of need for replacement) of amalgam and composite restorations in the Dutch adult popula...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345900690060901
更新日期：1990-06-01 00:00:00
