The induction of micronuclei in vitro by unpolymerized resin monomers.

Abstract:

:Components of resin materials may damage DNA, leading to genetic alterations in mammalian cells. Here, monomers were analyzed for the induction of chromosomal aberrations indicated by micronuclei induced in V79 cells. A dose-related increase in the numbers of micronuclei was observed with triethyleneglycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA), and glycidyl methacrylate (GMA). These effects were reduced, however, by a metabolically active microsomal fraction from rat liver. The very low activity of Bis-GMA and UDMA and the elevated numbers of micronuclei caused by high concentrations of methyl methacrylate and bisphenol A were associated with cytotoxicity. Our findings provide evidence for the induction of micronuclei by TEGDMA, HEMA, and GMA under physiological conditions, indicating clastogenic activity of these chemicals in vitro. Since it has been shown that TEGDMA also caused gene mutations and DNA sequence deletions in mammalian cells, the activity of this substance should be analyzed in vivo.

journal_name

J Dent Res

authors

Schweikl H,Schmalz G,Spruss T

doi

10.1177/00220345010800070401

keywords:

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

1615-20

issue

7

eissn

0022-0345

issn

1544-0591

journal_volume

80

pub_type

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