Abstract:
:Each odontoblast is tightly linked to other odontoblasts. They form a line of defense and are capable of withstanding external stimuli, particularly the inflammation caused by caries. Thus, we investigated exosomes derived from odontoblasts as an intercellular mechanism by which inflamed odontoblasts are protected from apoptosis. CD63, an exosome marker, was expressed at high levels in caries-affected regions of the dental pulp. We conducted an ex vivo experiment by applying different concentrations of lipopolysaccharide (LPS) to the odontoblast-like cells (mineralization was induced in stem cells derived from the apical papilla). Odontoblast-like cells treated with a high concentration of LPS (20 µg/mL LPS, severely affected) exhibited an accelerated release of exosomes, which attenuated the LPS-induced cell apoptosis of odontoblast-like cells treated with a low concentration of LPS (1 µg/mL LPS, mildly affected). Next, we blocked exosome uptake with chlorpromazine, and the rescue effect vanished. Based on our findings, severely inflamed odontoblasts attenuate the apoptosis of mildly inflamed neighboring cells through an exosome-mediated intercellular signaling pathway.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Wang HS,Yang FH,Wang YJ,Pei F,Chen Z,Zhang Ldoi
10.1177/0022034519869580subject
Has Abstractpub_date
2019-10-01 00:00:00pages
1271-1278issue
11eissn
0022-0345issn
1544-0591journal_volume
98pub_type
杂志文章abstract::Apoptosis plays important roles in various stages of organogenesis. In this study, we hypothesized that apoptosis would play an important role in tooth morphogenesis. We examined the role of apoptosis in early tooth development by using a caspase inhibitor, z-VAD-fmk, concomitant with in vitro organ culture and tooth ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910608500610
更新日期:2006-06-01 00:00:00
abstract::The objective of this study was to calculate the stress distribution induced in anterior metal-ceramic crowns fabricated with either gold-alloy or nickel-alloy copings of reduced thickness using plane stress analyses. Two-dimensional finite element models of three crown designs were subjected to a simulated biting for...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345860650091201
更新日期:1986-09-01 00:00:00
abstract::The aim of this meta-analysis, based on individual participant data from several studies, was to investigate the influence of patient-, materials-, and tooth-related variables on the survival of posterior resin composite restorations. Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA...
journal_title:Journal of dental research
pub_type: 杂志文章,meta分析,评审
doi:10.1177/0022034514544217
更新日期:2014-10-01 00:00:00
abstract::Multiple interactions occur among major determinants of dental caries. We have studied the bacterial flora and pH-lowering capacity of the same dental plaques in relation to caries. The findings on the plaque flora are reported here. The buccal surfaces of upper teeth in each subject were selected for study. A low-car...
journal_title:Journal of dental research
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doi:10.1177/00220345000790021201
更新日期:2000-02-01 00:00:00
abstract::The benefits of early orthodontic treatment are continuously discussed, but studies are few. We examined whether definite need for orthodontic treatment could be eliminated in public health care by systematically focusing on early intervention. One age cohort living in a rural Finnish municipality (N = 85) was regular...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700604
更新日期:2008-06-01 00:00:00
abstract::Patients with cleft lip/palate (CLP) have been reported, in some studies, to exhibit an increased prevalence of caries, although the underlying cause for this increase is unknown. In genetically defined mouse models, studies of postnatal sequelae associated with CLP have been hampered by neonatal lethality. Using a co...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034516679376
更新日期:2017-03-01 00:00:00
abstract::Epstein-Barr virus (EBV), in addition to its transforming properties, contributes to the pathogenesis of several inflammatory diseases. Here, we investigated its involvement in oral lichen planus (OLP), a common autoimmune-like disease of unknown etiopathogenesis that can display a malignant potential. EBV-infected ce...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034518788282
更新日期:2018-12-01 00:00:00
abstract::Rotational freedom of the implant-abutment connection influences its screw joint stability; for optimization, influential factors need to be evaluated based on a previously developed closed formula. The underlying hypothesis is that the manufacturing tolerances, geometric pattern, and dimensions of the index do not in...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034509341243
更新日期:2009-08-01 00:00:00
abstract::Interleukin (IL)-23 is an essential cytokine involved in expansion of the Th17 lineage, which is associated with many immune-related destructive tissue diseases. We hypothesized that the IL-23-induced Th17 pathway plays a role in periodontal pathology and examined the expression of cytokines, and related molecules, in...
journal_title:Journal of dental research
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doi:10.1177/0022034509339889
更新日期:2009-07-01 00:00:00
abstract::Selected strains of oral bacteria were analyzed for their ability to degrade wheat starch, maltose, maltotriose, and maltoheptaose. S. sanguis IUOM-11M and JC804, S. mutans 6715, S. salivarius IUOM-8, A. viscosus IUOM-62, and A. naeslundii ATCC 12104 degraded all four substrates. S. mutans NCTC 10449 degraded starch, ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345880670011501
更新日期:1988-01-01 00:00:00
abstract::Few studies have investigated smoking as a risk factor for root canal treatment. We studied the effect of smoking on the incidence of root canal treatment, controlling for recognized risk factors, in 811 dentate male participants in the VA Dental Longitudinal Study. Participants were not VA patients. Follow-up ranged ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910608500406
更新日期:2006-04-01 00:00:00
abstract::Defined sets of transcriptional factors can reprogram human somatic cells to induced pluripotent stem (iPS) cells. However, many types of human cells are not easily accessible to minimally invasive procedures. Here we evaluated dental pulp cells (DPCs) as an optimal source of iPS cells, since they are easily obtained ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034510366846
更新日期:2010-08-01 00:00:00
abstract::Medication-related osteonecrosis of the jaw (MRONJ), although initially believed to be exclusively associated with bisphosphonates, has been implicated in recent reports with additional drugs, especially the bone antiresorptive denosumab. The pathophysiology has not been fully elucidated, and no causal association bet...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034515572021
更新日期:2015-04-01 00:00:00
abstract::Although a secular decline is being seen in coronal caries in children in industrialized countries, there appears to be a relatively high prevalence of root caries, with chronologically older adults exhibiting the highest prevalences. This paper reviews the descriptive and analytical epidemiology of root caries as fou...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/00220345900690051901
更新日期:1990-05-01 00:00:00
abstract::The human dentition is a complex adaptive system that is influenced by genetic, epigenetic, and environmental factors. Within this system, is sexual dimorphism related to the growth promotion of the Y chromosome, or to hormonal influences, or both? This study is the first to investigate both primary and permanent toot...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034513484934
更新日期:2013-05-01 00:00:00
abstract::Previous studies have shown that fluoride affected the formation of apatite in spontaneous precipitations from unstable calcium phosphate solutions partly through its effect on the stability of such nonapatitic precursors as octacalcium phosphate, Ca8H2(PO4)6.5 H2O. The present study shows that fluoride had a comparab...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345800590021401
更新日期:1980-02-01 00:00:00
abstract::Sense of coherence (SOC) has been related to oral health behaviors and oral-health-related quality of life (OHRQoL) in observational studies. This cluster-randomized trial aimed to test the effect of an intervention to enhance SOC on OHRQoL in children. Twelve primary schools were randomly allocated to intervention an...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034512459757
更新日期:2013-01-01 00:00:00
abstract::Excessive mechanical stress (MS) during hyperocclusion is known to result in disappearance of the alveolar hard line, enlargement of the periodontal ligament (PDL) space, and destruction of alveolar bone, leading to occlusal traumatism. We hypothesized that MS induces expression of osteoclastogenesis-associated chemok...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034511400742
更新日期:2011-06-01 00:00:00
abstract::The fatigue crack growth resistance of dentin was characterized as a function of depth from the dentino-enamel junction. Compact tension (CT) specimens were prepared from the crowns of third molars in the deep, middle, and peripheral dentin. The microstructure was quantified in terms of the average tubule dimensions a...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034511408429
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abstract::Early-onset periodontitis (EOP) is considered to have a genetic basis which has not been clearly defined. Genetic polymorphisms of the vitamin D receptor (VDR-B-b) and the immunoglobulin-Fc-gamma receptor IIIb (FcgammaRIIIb-NA1-NA2) are associated with bone metabolism and infectious diseases, respectively. The purpose...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345010800120501
更新日期:2001-12-01 00:00:00
abstract::Improved mechanical properties of resin composites have been reported to be a consequence of the addition of aldehyde to the resins. The objective of the present study was to examine whether this improvement could be attributed to an increased degree of conversion of double bonds. For this purpose, propanal was added ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345940730101101
更新日期:1994-10-01 00:00:00
abstract::Dental enamel formation is a remarkable example of a biomineralization process. The exact mechanisms involved in this process remain partly obscure. Some of the genes encoding specific enamel proteins have been indicated as candidate genes for amelogenesis imperfecta. Mutational analyses within studied families have s...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/154405910508401206
更新日期:2005-12-01 00:00:00
abstract::We report a clinical study that examines whether HIV infection affects Streptococcus mutans colonization in the oral cavity. Whole stimulated saliva samples were collected from 46 HIV-seropositive individuals and 69 HIV-seronegative control individuals. The level of S. mutans colonization was determined by conventiona...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034512454298
更新日期:2012-09-01 00:00:00
abstract::The presence and kinetics of bone-resorbing activity in periapical lesions were studied with a rat model system. Lesions were found to expand most rapidly between induction on day 0 and day 15 ("active phase"), with enlargement occurring at a slower rate thereafter (days 20 and 30, "chronic phase"), as assessed by mea...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345910700100901
更新日期:1991-10-01 00:00:00
abstract::The tooth resistance measuring instrument was compared to the traditional method of the explorer examination for the detection of occlusal carious lesions. The findings of the instrument were more sensitive as compared to the traditional method when verified by a histological examination. ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345780570020801
更新日期:1978-02-01 00:00:00
abstract::Biological mediators have been used to enhance periodontal regeneration. The aim of this prospective randomized controlled study was to evaluate the safety and effectiveness of 3 doses of fibroblast growth factor 2 (FGF-2) when combined with a β-tricalcium phosphate (β-TCP) scaffold carrier placed in vertical infrabon...
journal_title:Journal of dental research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1177/0022034516632497
更新日期:2016-05-01 00:00:00
abstract::Adhesion of epithelium to the extracellular matrix is crucial for the maintenance of systemic and oral health. In the oral cavity, teeth or artificial dental implants penetrate the soft tissue of the gingiva. In this interface, gingival soft tissue needs to be well attached via the epithelial seal to the tooth or impl...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034511402207
更新日期:2011-12-01 00:00:00
abstract::Mixed or parotid saliva from caries-active individuals consistently supported better growth of Streptococcus mutans (type c) than that from caries-free individuals. Electrophoretic studies revealed that certain proteins in caries-active salivas were susceptible to microbial attack, but similar proteins in caries-free ...
journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:1979-10-01 00:00:00
abstract::Fluorides are present in the environment. Excessive systemic exposure to fluorides can lead to disturbances of bone homeostasis (skeletal fluorosis) and enamel development (dental/enamel fluorosis). The severity of dental fluorosis is also dependent upon fluoride dose and the timing and duration of fluoride exposure. ...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034510384626
更新日期:2011-05-01 00:00:00
abstract::Sealants are highly effective in preventing dental caries in the pits and fissures of teeth when applied by trained operators in clinical trials and public health programs. The effectiveness of fissure sealants when applied in dental practices is still not known. The purpose of this longitudinal study was to evaluate ...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章
doi:10.1177/00220345950740091301
更新日期:1995-09-01 00:00:00