Dental pulp cells for induced pluripotent stem cell banking.

Abstract:

:Defined sets of transcriptional factors can reprogram human somatic cells to induced pluripotent stem (iPS) cells. However, many types of human cells are not easily accessible to minimally invasive procedures. Here we evaluated dental pulp cells (DPCs) as an optimal source of iPS cells, since they are easily obtained from extracted teeth and can be expanded under simple culture conditions. From all 6 DPC lines tested with the conventional 3 or 4 reprogramming factors, iPS cells were effectively established from 5 DPC lines. Furthermore, determination of the HLA types of 107 DPC lines revealed 2 lines homozygous for all 3 HLA loci and showed that if an iPS bank is established from these initial pools, the bank will cover approximately 20% of the Japanese population with a perfect match. Analysis of these data demonstrates the promising potential of DPC collections as a source of iPS cell banks for use in regenerative medicine.

journal_name

J Dent Res

authors

Tamaoki N,Takahashi K,Tanaka T,Ichisaka T,Aoki H,Takeda-Kawaguchi T,Iida K,Kunisada T,Shibata T,Yamanaka S,Tezuka K

doi

10.1177/0022034510366846

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

773-8

issue

8

eissn

0022-0345

issn

1544-0591

pii

0022034510366846

journal_volume

89

pub_type

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