Genome-wide Identification of Foxf2 Target Genes in Palate Development.


:Cleft palate is among the most common structural birth defects in humans. Previous studies have shown that mutations in FOXF2 are associated with cleft palate in humans and mice and that Foxf2 acts in a Shh-Foxf-Fgf18-Shh molecular network controlling palatal shelf growth. In this study, we combined RNA-seq and ChIP-seq approaches to identify direct transcriptional target genes mediating Foxf2 function in palate development in mice. Of 155 genes that exhibited Foxf2-dependent expression in the developing palatal mesenchyme, 88 contained or were located next to Foxf2-binding sites. Through in situ hybridization analyses, we demonstrate that expression of many of these target genes, including multiple genes encoding transcription factors and several encoding extracellular matrix-modifying proteins, were specifically upregulated in the posterior region of palatal shelves in Foxf2-/- mouse embryos. Foxf2 occupancy at many of these putative target loci, including Fgf18, in the developing palatal tissues was verified by ChIP-polymerase chain reaction analyses. One of the Foxf2 target genes, Chst2, encodes a carbohydrate sulfotransferase integral to glycosaminoglycan sulfation. Correlating with ectopic Chst2 expression, Foxf2-/- embryos a exhibited region-specific increase in sulfated keratan sulfate and a concomitant reduction in chondroitin sulfate accumulation in the posterior palatal mesenchyme. However, expression of the core protein of versican, a major chondroitin sulfate proteoglycan important in palatal shelf morphogenesis, was increased, whereas expression of collagen I was reduced in the corresponding region of the palatal mesenchyme. These results indicate that, in addition to regulating palatal shelf growth through the Fgf18-Shh signaling network, Foxf2 controls palatal shelf morphogenesis through regulating expression of multiple transcription factors as well as through directly controlling the synthesis and processing of extracellular matrix components in the palatal mesenchyme. Our ChIP-seq and RNA-seq data sets provide an excellent resource for comprehensive understanding of the molecular network controlling palate development.


J Dent Res


Xu J,Liu H,Lan Y,Park JS,Jiang R




Has Abstract


2020-04-01 00:00:00












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    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Galindo Y,McLachlan K,Kasloff Z

    更新日期:1980-02-01 00:00:00

  • Glass-ionomer cement formulations. II. The synthesis of novel polycarobxylic acids.

    abstract::The synthesis of many polycarboxylic acids is reported. An account is given of their stability in aqueous solution and the properties of cements formed by their reaction with ion-leachable glasses. A copolymer of acrylic and itaconic acids was found to combine several favorable characteristics. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Crisp S,Kent BE,Lewis BG,Ferner AJ,Wilson AD

    更新日期:1980-06-01 00:00:00

  • Probiotics reduce the prevalence of oral candida in the elderly--a randomized controlled trial.

    abstract::Overgrowth of oral yeast is a common problem among the elderly. Probiotic bacteria are known to inhibit the growth of pathogenic microbes. We tested the hypothesis that cheese containing probiotic bacteria can reduce the prevalence of oral Candida. During this 16-week, randomized, double-blind, placebo-controlled stud...

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    authors: Hatakka K,Ahola AJ,Yli-Knuuttila H,Richardson M,Poussa T,Meurman JH,Korpela R

    更新日期:2007-02-01 00:00:00

  • Temperature ofthe pulp chamber during impression taking of full crown preparations with modelling compound.

    abstract::Temperature elevations were measured in the pulp chamber of full cast crown preparations during impression taking with modelling compound in copper bands. Uncontrolled flaming, controlled flaming, and water heating of the bands produced temperatures of 53, 44, and 41.5 C, respectively. The time involved in restoring t...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Grajower R,Kaufman E,Stern N

    更新日期:1975-03-01 00:00:00

  • The growth hormone receptor gene is associated with mandibular height in a Chinese population.

    abstract::Genetic influences are important in the determination of mandibular morphology, and growth hormone receptor (GHR) is believed to have an important influence on the growth of craniofacial bone. In this study, we used quantitative trait locus methods to evaluate the relationship between craniofacial morphology and singl...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Zhou J,Lu Y,Gao XH,Chen YC,Lu JJ,Bai YX,Shen Y,Wang BK

    更新日期:2005-11-01 00:00:00

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    abstract::Vietnamese immigrant and Norwegian children, matched according to caries experience, age, and sex, were compared with respect to S. mutans in dental plaque and in saliva. Regardless of ethnic group, S. mutans prevalence was highest in plaque over caries lesions and from fissures, and lowest in plaque from smooth surfa...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Scheie AA,Selikowitz HS,Arneberg P

    更新日期:1984-12-01 00:00:00

  • Selection of an experimental unit in rat caries studies.

    abstract::In two separate experiments, none of the 14 subunits examined consistently ranked dentifrices similarly to the ranking obtained when the individual rat was used as the experimental unit. Arbitrarily dividing the rat into subunits and then comparing results among these subunits may lead to false conclusions since the r...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Briner WW

    更新日期:1976-05-01 00:00:00

  • Correlations of the expression of fibroblast growth factor-2, vascular endothelial growth factor, and their receptors with angiogenesis in synovial tissues from patients with internal derangement of the temporomandibular joint.

    abstract::Synovitis in internal derangement of the temporomandibular joint (TMJ) is accompanied by the growth of new blood vessels. Fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factor (VEGF) are well-characterized angiogenic factors. The objective of this study was to elucidate the correlation between the ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Sato J,Segami N,Yoshitake Y,Nishikawa K

    更新日期:2003-04-01 00:00:00

  • Genetic covariance structure of incisor crown size in twins.

    abstract::Previous studies of tooth size in twins and their families have suggested a high degree of genetic control, although there have been difficulties separating the various genetic and environmental effects. A genetic analysis of variation in crown size of the permanent incisors of South Australian twins was carried out, ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Dempsey PJ,Townsend GC,Martin NG,Neale MC

    更新日期:1995-07-01 00:00:00

  • Post placement affects survival of endodontically treated premolars.

    abstract::Clinical evidence is lacking regarding the influence of the amount of residual coronal dentin and of post placement on the failure risk of endodontically compromised teeth. The aim of this prospective clinical trial was to assess whether these factors significantly affect the two-year survival of restored pulpless pre...

    journal_title:Journal of dental research

    pub_type: 杂志文章,随机对照试验


    authors: Ferrari M,Cagidiaco MC,Grandini S,De Sanctis M,Goracci C

    更新日期:2007-08-01 00:00:00

  • Caries management strategies for primary molars: 1-yr randomized control trial results.

    abstract::Minimal invasive approaches to managing caries, such as partial caries removal techniques, are showing increasing evidence of improved outcomes over the conventional complete caries removal. There is also increasing interest in techniques where no caries is removed. We present the 1-yr results of clinical efficacy for...

    journal_title:Journal of dental research

    pub_type: 杂志文章,随机对照试验


    authors: Santamaria RM,Innes NP,Machiulskiene V,Evans DJ,Splieth CH

    更新日期:2014-11-01 00:00:00

  • The resistance and adaptation of selected oral bacteria to mercury and its impact on their growth.

    abstract::Selected strains of oral Streptococcus and Actinomyces have been tested for their ability to grow in the presence of mercury. Strains were tested for growth on a semi-defined medium with low mercury-binding characteristics. Sensitivities were initially measured on agar plates, and subsequently, selected strains were g...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Lyttle HA,Bowden GH

    更新日期:1993-09-01 00:00:00

  • In vivo occlusal forces and moments: I. Forces measured in terminal hinge position and associated moments.

    abstract::Microducers and modified cephalometric roentgenogram techniques were used to study maximum biting forces and moments in an adult male. The force at the second molar was approximately 10% greater than that at the first molar. At the second molar, the moment of force was approximately 15% less than its value at the firs...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Mansour RM,Reynik RJ

    更新日期:1975-01-01 00:00:00

  • Antimalarial drugs and reduced incidence of dental plaque.

    abstract::Known antimalarial compunds from several chemical classes were tested for their ability to inhibit in vitro growth of microorganisms causing dental plaque and to prevent the development of plaque in a hamster model. Approximately a third of the compounds tested inhibited in vitro growth; however, none prevented develo...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Kinnamon KE,Eckermann EH,Tempel TR,Smith JH,Wolf CR

    更新日期:1975-05-01 00:00:00

  • Proteolysis by MMP20 Prevents Aberrant Mineralization in Secretory Enamel.

    abstract::The present study was conducted to investigate the role of proteolysis by matrix metalloproteinase 20 (MMP20) in regulating the initial formation of the enamel mineral structure during the secretory stage of amelogenesis, utilizing Mmp20-null mice that lack this essential protease. Ultrathin sagittal sections of maxil...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Yamazaki H,Tran B,Beniash E,Kwak SY,Margolis HC

    更新日期:2019-04-01 00:00:00

  • Rare bone diseases and their dental, oral, and craniofacial manifestations.

    abstract::Hereditary diseases affecting the skeleton are heterogeneous in etiology and severity. Though many of these conditions are individually rare, the total number of people affected is great. These disorders often include dental-oral-craniofacial (DOC) manifestations, but the combination of the rarity and lack of in-depth...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: Foster BL,Ramnitz MS,Gafni RI,Burke AB,Boyce AM,Lee JS,Wright JT,Akintoye SO,Somerman MJ,Collins MT

    更新日期:2014-07-01 00:00:00

  • Oral manifestations of Sjögren's syndrome.

    abstract::Sjögren's syndrome is a common autoimmune rheumatic disease. The most common symptoms of Sjögren's syndrome are extreme tiredness, along with dry eyes (keratoconjunctivitis sicca) and dry mouth (xerostomia). Saliva plays an essential role in numerous functions of the mouth. Xerostomia can be caused by medications, chr...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: Mathews SA,Kurien BT,Scofield RH

    更新日期:2008-04-01 00:00:00

  • Activin and Bmp4 Signaling Converge on Wnt Activation during Odontogenesis.

    abstract::Previous studies show that both activin and Bmp4 act as crucial mesenchymal odontogenic signals during early tooth development. Remarkably, mice lacking activin-βA ( Inhba-/-) and mice with neural crest-specific inactivation of Bmp4 ( Bmp4ncko/ncko) both exhibit bud-stage developmental arrest of the mandibular molar t...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Kwon HE,Jia S,Lan Y,Liu H,Jiang R

    更新日期:2017-09-01 00:00:00

  • Effect of xylitol consumption on the plaque-saliva distribution of mutans streptococci and the occurrence and long-term survival of xylitol-resistant strains.

    abstract::Since the exposure of mutans streptococci to xylitol is known to select for xylitol-resistant (XR) natural mutants, the occurrence and long-term survival of such xylitol-resistant strains was evaluated in a cross-sectional sampling of participants of the Ylivieska xylitol study four years after the original two-year e...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Trahan L,Söderling E,Dréan MF,Chevrier MC,Isokangas P

    更新日期:1992-11-01 00:00:00

  • Induced hypoxia in rat pulp and periapex demonstrated by 3H-misonidazole retention.

    abstract::Cellular hypoxia may be a useful indication of tissue distress in the dental pulp that could be used to investigate the early stages of pulpal responses. Tritiated misonidazole (3H-MISO) is a marker which preferentially labels cells with decreased oxygen tension (hypoxia). The experiments reported here were carried ou...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Baumgardner KR,Walton RE,Osborne JW,Born JL

    更新日期:1996-10-01 00:00:00

  • Estimation of mesiodistal width of permanent canines and premolars in early mixed dentition.

    abstract::The accuracy for predicting mesiodistal widths of unerupted permanent canines and premolars from x-rays and their estimations based on the already erupted permanent teeth were tested in a group of Israeli children. The observed posteruptive widths related more closely to predicted values obtained from x-ray measuremen...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Zilberman Y,Koyoumdjisky-Kaye E,Vardimon A

    更新日期:1977-08-01 00:00:00

  • Glass-ionomer restoratives: a systematic review of a secondary caries treatment effect.

    abstract::It is generally accepted that glass ionomers inhibit secondary caries in vivo, and data from in vitro studies support this effect. The aim of this review was a systematic assessment, from the literature, of clinical evidence for the ability of glass-ionomer restoratives to inhibit secondary caries at the restoration m...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: Randall RC,Wilson NH

    更新日期:1999-02-01 00:00:00

  • Analysis of blood cortisol levels in oral surgery patients given various levels of intravenous medication.

    abstract::Blood cortisol levels of oral surgery patients were analyzed to objectively determine the effect of intravenous sedation on surgical stress. Results showed a measure of sedation but did not adequately reflect the stress of oral surgery or the changes produced by intravenous sedation. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Bunch FL,Allen GD,Jorgensen NB

    更新日期:1975-09-01 00:00:00

  • A new approach to the study of tooth wear.

    abstract::Human tooth wear occurs so slowly that traditionally it has needed months or years to be measurable. This study showed that microscopic changes in wear patterns on human teeth could be detected in a matter of days and could be used as indicators of rates of wear. Thus, daily or weekly changes in rates of wear can be d...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Teaford MF,Tylenda CA

    更新日期:1991-03-01 00:00:00

  • Porphyromonas gingivalis Induces Insulin Resistance by Increasing BCAA Levels in Mice.

    abstract::Insulin resistance is one of the critical pathogeneses of type 2 diabetes mellitus (T2DM). Elevated levels of plasma branched-chain amino acids (BCAAs) are associated with insulin resistance. Recent studies have demonstrated the role of Porphyromonas gingivalis in the development of insulin resistance. However, the me...

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    authors: Tian J,Liu C,Zheng X,Jia X,Peng X,Yang R,Zhou X,Xu X

    更新日期:2020-07-01 00:00:00

  • Association between Bone Mass and Dental Hypomineralization.

    abstract::The aim of this study was to examine the association between the bone mass (bone mineral content [BMC]) and hypomineralized second primary molars (HSPMs)/molar incisor hypomineralization (MIH) in 6-y-old children. This cross-sectional study was embedded in the Generation R Study, a population-based prospective cohort ...

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    authors: van der Tas JT,Elfrink ME,Vucic S,Heppe DH,Veerkamp JS,Jaddoe VW,Rivadeneira F,Hofman A,Moll HA,Wolvius EB

    更新日期:2016-04-01 00:00:00

  • Effects of various resin composite (co)monomers and extracts on two caries-associated micro-organisms in vitro.

    abstract::Previous studies have shown that extracts of various filling materials, e.g., resin composites, may influence the growth of cariogenic micro-organisms. The purpose of this study was to examine the effects of important resin composite (co)monomers (Bis-GMA, UDMA, TEGDMA, EGDMA) and extracts of two commercial dental com...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Hansel C,Leyhausen G,Mai UE,Geurtsen W

    更新日期:1998-01-01 00:00:00

  • Refractive index mismatch and monomer reactivity influence composite curing depth.

    abstract::Limited cure depth is a drawback of light-activated composites. We hypothesize that curing light transmission and cure depth are influenced by monomer reactivity and filler/resin refractive index mismatch. Light transmission throughout cure was recorded for composites based on strontium (refractive index 1.51) or bari...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Shortall AC,Palin WM,Burtscher P

    更新日期:2008-01-01 00:00:00

  • Incorporation of bacterial inhibitor into resin composite.

    abstract::Attempts to produce resin composite with antibacterial properties by incorporation of an antibacterial agent such as chlorhexidine have been reported, but problems can arise due to release of the inhibitory agent from the composite. Such problems may include toxic effects, influence on mechanical properties, and loss ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Imazato S,Torii M,Tsuchitani Y,McCabe JF,Russell RR

    更新日期:1994-08-01 00:00:00

  • The expression of dentin sialophosphoprotein gene in bone.

    abstract::Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript coding for a large precursor protein termed dentin sialophosphoprotein (DSPP). DSP, DPP, and DSPP have been considered to be tooth-specific. To test for the expression of the dspp gene in bone, we performed Western immu...

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    authors: Qin C,Brunn JC,Cadena E,Ridall A,Tsujigiwa H,Nagatsuka H,Nagai N,Butler WT

    更新日期:2002-06-01 00:00:00