Genome-wide Identification of Foxf2 Target Genes in Palate Development.

Abstract:

:Cleft palate is among the most common structural birth defects in humans. Previous studies have shown that mutations in FOXF2 are associated with cleft palate in humans and mice and that Foxf2 acts in a Shh-Foxf-Fgf18-Shh molecular network controlling palatal shelf growth. In this study, we combined RNA-seq and ChIP-seq approaches to identify direct transcriptional target genes mediating Foxf2 function in palate development in mice. Of 155 genes that exhibited Foxf2-dependent expression in the developing palatal mesenchyme, 88 contained or were located next to Foxf2-binding sites. Through in situ hybridization analyses, we demonstrate that expression of many of these target genes, including multiple genes encoding transcription factors and several encoding extracellular matrix-modifying proteins, were specifically upregulated in the posterior region of palatal shelves in Foxf2-/- mouse embryos. Foxf2 occupancy at many of these putative target loci, including Fgf18, in the developing palatal tissues was verified by ChIP-polymerase chain reaction analyses. One of the Foxf2 target genes, Chst2, encodes a carbohydrate sulfotransferase integral to glycosaminoglycan sulfation. Correlating with ectopic Chst2 expression, Foxf2-/- embryos a exhibited region-specific increase in sulfated keratan sulfate and a concomitant reduction in chondroitin sulfate accumulation in the posterior palatal mesenchyme. However, expression of the core protein of versican, a major chondroitin sulfate proteoglycan important in palatal shelf morphogenesis, was increased, whereas expression of collagen I was reduced in the corresponding region of the palatal mesenchyme. These results indicate that, in addition to regulating palatal shelf growth through the Fgf18-Shh signaling network, Foxf2 controls palatal shelf morphogenesis through regulating expression of multiple transcription factors as well as through directly controlling the synthesis and processing of extracellular matrix components in the palatal mesenchyme. Our ChIP-seq and RNA-seq data sets provide an excellent resource for comprehensive understanding of the molecular network controlling palate development.

journal_name

J Dent Res

authors

Xu J,Liu H,Lan Y,Park JS,Jiang R

doi

10.1177/0022034520904018

subject

Has Abstract

pub_date

2020-04-01 00:00:00

pages

463-471

issue

4

eissn

0022-0345

issn

1544-0591

journal_volume

99

pub_type

杂志文章
  • Sugar Consumption and Changes in Dental Caries from Childhood to Adolescence.

    abstract::There are no prospective studies investigating the effects of sugar-related feeding practices on changes in dental caries from early childhood to young adulthood. The aim of this study was to assess whether sugar-related feeding practices affect dental caries between the ages of 6 and 18 y. This birth cohort study was...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034515625907

    authors: Peres MA,Sheiham A,Liu P,Demarco FF,Silva AE,Assunção MC,Menezes AM,Barros FC,Peres KG

    更新日期:2016-04-01 00:00:00

  • Characterization of dental pulp stem cells of human tooth germs.

    abstract::In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910808700716

    authors: Takeda T,Tezuka Y,Horiuchi M,Hosono K,Iida K,Hatakeyama D,Miyaki S,Kunisada T,Shibata T,Tezuka K

    更新日期:2008-07-01 00:00:00

  • YAP overexpression affects tooth morphogenesis and enamel knot patterning.

    abstract::Teeth develop through distinct morphological stages. At the cap stage, a compactly clustered and concentrically arranged cell mass, the enamel knot, appears at the tip of the enamel organ. Cells in this knot express sets of key molecules, and as such have been proposed to act as a signaling center directing tooth morp...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034514525784

    authors: Liu M,Zhao S,Wang XP

    更新日期:2014-05-01 00:00:00

  • 4-META opaque resin--a new resin strongly adhesive to nickel-chromium alloy.

    abstract::1) A new adhesive opaque resin containing a reactive monomer, 4-methacryloxy-ethyl trimellitate anhydride (4-META), was prepared, and its application to thermosetting acrylic resin veneer crowns was studied. 2) The 4-META opaque resin was applied to a variety of nickel-chromium dental alloy specimens which had undergo...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345810600091101

    authors: Tanaka T,Nagata K,Takeyama M,Atsuta M,Nakabayashi N,Masuhara E

    更新日期:1981-09-01 00:00:00

  • A novel role for Twist-1 in pulp homeostasis.

    abstract::The molecular mechanisms that maintain the equilibrium of odontoblast progenitor cells in dental pulp are unknown. Here we tested whether homeostasis in dental pulp is modulated by Twist-1, a nuclear protein that partners with Runx2 during osteoblast differentiation. Our analysis of Twist-1(+/-) mice revealed phenotyp...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910708601007

    authors: Galler KM,Yasue A,Cavender AC,Bialek P,Karsenty G,D'Souza RN

    更新日期:2007-10-01 00:00:00

  • Low metacarpal bone density, tooth loss, and periodontal disease in Japanese women.

    abstract::The relationship between periodontitis and systemic bone mineral density in Japanese women is undetermined. We tested the hypothesis that periodontitis was more frequent in women with low metacarpal bone mineral density (m-BMD). Subjects were 190 Japanese women (89 premenopausal, 101 post-menopausal). Periodontal stat...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345010800090901

    authors: Inagaki K,Kurosu Y,Kamiya T,Kondo F,Yoshinari N,Noguchi T,Krall EA,Garcia RI

    更新日期:2001-09-01 00:00:00

  • Gene polymorphisms and the prevalence of key periodontal pathogens.

    abstract::Growing evidence suggests that individual genetic susceptibility may influence the host's response to infections. The aim of this project was to study whether gene polymorphisms of inflammatory markers are associated with the presence of viable periodontopathogenic bacteria. We extracted genomic DNA from 45 young adul...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910708600505

    authors: Nibali L,Ready DR,Parkar M,Brett PM,Wilson M,Tonetti MS,Griffiths GS

    更新日期:2007-05-01 00:00:00

  • Functional characteristics of atrophic parotid acinar cells from rats after liquid feeding.

    abstract::Rat parotid atrophy, induced by liquid feeding over 10 days, was manifested as gland weight loss (40%) and histologically as acinar shrinkage. Acinar secretory function was investigated in the same glands using enzymatically dispersed cell preparations and superfused gland slices. Results were normalized for the acina...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345940730060901

    authors: Scott J,Gunn DL

    更新日期:1994-06-01 00:00:00

  • Antigens and surface components associated with virulence of Actinomyces viscosus.

    abstract::We have isolated a specific cell wall antigen of high molecular weight which appears to be unique to virulent strains of A viscosus and A naeslundii. The antigen is composed of two parts: a polysaccharide moiety containing 6-DOT as the major sugar and determinant of serologic specificity, and a small peptide bearing s...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/002203457605500111011

    authors: Hammond BF,Steel CF,Peindl KS

    更新日期:1976-01-01 00:00:00

  • Effect of alpha-receptor stimulation on Cl transport by rat submandibular acini.

    abstract::Dispersed salivary acini isolated from the rat submandibular gland by enzymatic digestion were used to study the effects of alpha-receptor stimulation on transmembrane transport of 36Cl. In the absence of secretagogue, the tracer accumulated in the cells in a time-dependent manner until a steady-state content of 6.8 +...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345880670030701

    authors: Martinez JR,Reed P

    更新日期:1988-03-01 00:00:00

  • Self-perceived oral health status, psychological well-being, and life satisfaction in an older adult population.

    abstract::Numerous studies have demonstrated that many older adults have problems chewing, pain, difficulties in eating, and problems in social relationships because of oral disorders. However, it is not clear if these functional and psychosocial outcomes affect broader psychological well-being and life satisfaction. Consequent...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345000790041301

    authors: Locker D,Clarke M,Payne B

    更新日期:2000-04-01 00:00:00

  • Craniofacial and TMJ effects after glutamate and TRH microsphere implantation in proximity to trigeminal motoneurons of growing rats.

    abstract::The sequelae of sustained, in vivo delivery of two important neurotransmitter substances, glutamate and thyrotropin-releasing hormone (TRH), upon craniofacial growth and development have previously not been investigated. Our purpose was to document and compare the relative effects of glutamate and TRH microspheres ste...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345970760080601

    authors: Byrd KE,Sukay MJ,Dieterle MW,Yang L,Marting TC,Teomim D,Domb AJ

    更新日期:1997-08-01 00:00:00

  • Trigeminal nociceptors express TLR-4 and CD14: a mechanism for pain due to infection.

    abstract::Although certain bacterial species appear to be risk factors for pain due to odontogenic infections, comparatively little is known about the potential mechanisms mediating this effect. In this study, we tested the hypothesis that trigeminal nociceptive neurons express the TLR4 or CD14 receptors, thus enabling sensory ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910608500108

    authors: Wadachi R,Hargreaves KM

    更新日期:2006-01-01 00:00:00

  • Experimental observation of silver and gold penetration into dental ceramic by means of a radiotracer technique.

    abstract::A radiotracer technique was used to study silver and gold diffusion into dental porcelain under experimental conditions close to the real conditions in prosthetic laboratories for porcelain bakes. It was clearly shown that these non-oxidizable elements were able to diffuse into the ceramic as well as oxidizable ones. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345870660120401

    authors: Moya F,Payan J,Bernardini J,Moya EG

    更新日期:1987-12-01 00:00:00

  • Particle size distribution of food boluses after mastication of six natural foods.

    abstract::There is a large variability between and among individuals in the physiology of mastication, but it is not known whether this produces a similar variability in the particle sizes of food boluses at the end of the chewing process. Food boluses obtained just before swallowing were analyzed in ten subjects (aged 36.7 +/-...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章

    doi:10.1177/154405910408300713

    authors: Peyron MA,Mishellany A,Woda A

    更新日期:2004-07-01 00:00:00

  • Dentists' judgment strategies on prophylactic removal of mandibular third molars.

    abstract::The number of molars selected for prophylactic removal varies widely among general dental practitioners and oral surgeons. To understand the basis for such variations, we investigated two hypotheses: (1) Individual judgment strategies will differ concerning the use of cues (items of information), and (2) few dentists ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345000790121101

    authors: Knutsson K,Lysell L,Rohlin M

    更新日期:2000-12-01 00:00:00

  • Adrenergic signaling in human oral keratinocytes and wound repair.

    abstract::Catecholamines are present in saliva, but their influence on oral epithelium is not understood. Because psychological stress increases salivary catecholamines and impairs oral mucosal wound healing, we sought to determine if epithelial adrenergic signaling could link these two findings. We found that cultured human or...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034510388034

    authors: Steenhuis P,Huntley RE,Gurenko Z,Yin L,Dale BA,Fazel N,Isseroff RR

    更新日期:2011-02-01 00:00:00

  • An explanation for the "two fiber axes" problem in human enamel by x-ray diffraction.

    abstract::Since Thewlis found the "two fiber axes" patterns on x-ray diffractograms of human enamel in 1934, many investigators have studied this problem. However, a definite answer has not yet been obtained. The present detailed x-ray diffraction study showed that the "two fiber axes" patterns in human enamel were caused by re...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345860650071601

    authors: Hirota F

    更新日期:1986-07-01 00:00:00

  • Adherent interactions which may affect microbial ecology in the mouth.

    abstract::Bacteria attach to teeth and oral mucosal surfaces in a surprisingly selective manner, and attachment appears to be the first step in the colonization process. Attachment is thought to involve lectin-like and/or hydrophobic ligands, called adhesins, often present in bacterial surface appendages which interact with rec...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630030401

    authors: Gibbons RJ

    更新日期:1984-03-01 00:00:00

  • Caries-Associated Biosynthetic Gene Clusters in Streptococcus mutans.

    abstract::Early childhood caries (ECC) is a chronic disease affecting the oral health of children globally. This disease is multifactorial, but a primary factor is cariogenic microorganisms such as Streptococcus mutans. Biosynthetic gene clusters (BGCs) encode small molecules with diverse biological activities that influence th...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034520914519

    authors: Momeni SS,Beno SM,Baker JL,Edlund A,Ghazal T,Childers NK,Wu H

    更新日期:2020-07-01 00:00:00

  • Trends in Dental Implant Use in the U.S., 1999-2016, and Projections to 2026.

    abstract::Dental implants have become an increasingly popular treatment choice for replacing missing teeth. Yet, little is known about the prevalence and sociodemographic distribution of dental implant use in the United States. To address this knowledge gap, we analyzed data from 7 National Health and Nutrition Examination Surv...

    journal_title:Journal of dental research

    pub_type: 历史文章,杂志文章

    doi:10.1177/0022034518792567

    authors: Elani HW,Starr JR,Da Silva JD,Gallucci GO

    更新日期:2018-12-01 00:00:00

  • Langerhans cells from oral epithelium are more effective in stimulating allogeneic t-cells in vitro than Langerhans cells from skin epithelium.

    abstract::Dendritic cells, such as Langerhans cells (LC), in different ectodermal compartments may have different functional capabilities. The present study was undertaken to compare oral Langerhans cells (LC) with those of the epidermis in terms of their ability to co-stimulate T-cells in vitro. A Mixed Epithelial Cell Lymphoc...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345990780030701

    authors: Hasséus B,Jontell M,Bergenholtz G,Eklund C,Dahlgren UI

    更新日期:1999-03-01 00:00:00

  • A model system to demonstrate the role of cavitational activity in ultrasonic scaling.

    abstract::A model system consisting of vacuum-deposited aluminum on glass was developed to demonstrate the effectiveness of cavitational activity (occurring within the cooling water supply of a dental ultrasonic scaler) in the removal of particulate material from solid surfaces. The amount of solid material removed from the mod...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630091401

    authors: Walmsley AD,Laird WR,Williams AR

    更新日期:1984-09-01 00:00:00

  • Etching patterns of Co-Cr alloys for bonded cast restorations.

    abstract::Resin-bonded bridges may replace missing teeth and act as splints in periodontal treatment. The objective of this study was to investigate the etch pattern after electrolytic etching of selected Co-Cr alloys in hydrochloric acid and to assess the changes in alloy composition after different etching times. The alloys i...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345870660091301

    authors: Ekstrand K,Ruyter IE

    更新日期:1987-09-01 00:00:00

  • Effect of Biomechanical Environment on Degeneration of Meckel's Cartilage.

    abstract::During orofacial tissue development, the anterior and posterior regions of the Meckel's cartilage undergo mineralization, while the middle region undergoes degeneration. Despite the interesting and particular phenomena, the mechanisms that regulate the different fates of Meckel's cartilage, including the effects of bi...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034520960118

    authors: Farahat M,Kazi GAS,Hara ES,Matsumoto T

    更新日期:2021-02-01 00:00:00

  • Fluoride deposition in human enamel in vivo from professionally applied fluoride prophylaxis paste.

    abstract::Deciduous teeth were collected from children who received professional prophylaxis with an acidulated phosphate-fluoride (APF) or placebo paste and were analyzed to determine fluoride uptake. Enamel fluoride level was increased by the APF paste approximately 300 ppm at a depth of 2.5 micrometers during the first month...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345760550064901

    authors: Mellberg JR,Nicholson CR,Ripa LW,Barenie J

    更新日期:1976-11-01 00:00:00

  • Kinetic aspects of gingival and periodontal ligament fibroblast attachment to surface-conditioned dentin.

    abstract::An in vitro model has been developed for measurement of initial attachment of 3H-labeled human gingival fibroblasts (HGF) and periodontal ligament cells (HPDL cells) to sieved dentin powder. Reproducible attachment was obtained and was closely related to the number of plated cells, the amount of dentin powder, the tem...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345930720030901

    authors: Rompen EH,Kohl J,Nusgens B,Lapiere CM

    更新日期:1993-03-01 00:00:00

  • Impact of Obesity on Orthodontic Tooth Movement in Adolescents: A Prospective Clinical Cohort Study.

    abstract::Obesity is a widespread chronic inflammatory disorder characterized by an increased overall disease burden and significant association with periodontitis. The aim of this prospective clinical cohort study was to investigate the effect of obesity on orthodontic tooth movement. Fifty-five adolescent patients (27 males, ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034516688448

    authors: Saloom HF,Papageorgiou SN,Carpenter GH,Cobourne MT

    更新日期:2017-05-01 00:00:00

  • Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.

    abstract::The role of Streptococcus mutans in the initiation of dental caries has been recognized and attributed, at least in part, to its ability to colonize the tooth surface. Therefore, factors which prevent S. mutans attachment to hydroxyapatite (HA) are of considerable interest for the prophylaxis of this infectious diseas...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345970760020701

    authors: Tarsi R,Muzzarelli RA,Guzmán CA,Pruzzo C

    更新日期:1997-02-01 00:00:00

  • Oral Implants: The Paradigm Shift in Restorative Dentistry.

    abstract::The discovery of the phenomenon "osseointegration," or functional ankylosis, has led to the development of oral implants with high clinical performance. Consequently, the placement of titanium implants has changed the paradigms of restorative dentistry. Implants are used to prevent placing reconstructions anchored on ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034519853574

    authors: Lang NP

    更新日期:2019-11-01 00:00:00