Abstract：
:The oral microbiome consists of a planktonic microbiome residing in saliva and an adhering microbiome (the biofilm adhering to oral hard and soft tissues). Here we hypothesized that possible differences in microbial composition of the planktonic and adhering oral microbiome on teeth can be related to the forces by which different bacterial species are attracted to the tooth surface. The relative presence of 7 oral bacterial species in saliva and biofilm collected from 10 healthy human volunteers was determined twice in each volunteer by denaturing-gradient-gel electrophoresis. Analysis of both microbiomes showed complete separation of the planktonic from the adhering oral microbiome. Next, adhesion forces of corresponding bacterial strains with saliva-coated enamel surfaces were measured by atomic force microscopy. Species that were found predominantly in the adhering microbiome had significantly higher adhesion forces to saliva-coated enamel (-0.60 to -1.05 nN) than did species mostly present in the planktonic microbiome (-0.40 to -0.55 nN). It is concluded that differences in composition of the planktonic and the adhering oral microbiome are due to small differences in the forces by which strains adhere to saliva-coated enamel, providing an important step in understanding site- and material-specific differences in the composition of biofilms in the oral cavity.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Wessel SW,Chen Y,Maitra A,van den Heuvel ER,Slomp AM,Busscher HJ,van der Mei HCdoi
10.1177/0022034513511822subject
Has Abstractpub_date
2014-01-01 00:00:00pages
84-8issue
1eissn
0022-0345issn
1544-0591pii
0022034513511822journal_volume
93pub_type
杂志文章abstract：:Bisphosphonates are a class of agents used to treat osteoporosis and malignant bone metastases. The efficacy of these agents in treating and preventing the significant skeletal complications associated with these conditions has had a major positive impact for patients and is responsible for their widespread use in med...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/154405910708601101
更新日期：2007-11-01 00:00:00
abstract：:Amylase activity in various tissues--i.e., submandibular/sublingual and parotid glands, the pancreas, saliva, and serum--in rats was measured after injection of isoproterenol, pilocarpine, and acetylcholine. All agents reduced amylase activity in the parotid gland and increased the enzyme activity in the submandibular...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345830620011201
更新日期：1983-01-01 00:00:00
abstract：:Masticatory muscle silent periods were compared in normal subjects and in patients with MPD syndrome before and after a variety of psychological, physiological, and pharmacological forms of therapy. All patients initially showed prolonged silent periods in one or more of the muscles. There was a direct relationship be...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600030501
更新日期：1981-03-01 00:00:00
abstract：:Weanling rice rats were fed either standard laboratory chow or Diet 2000. The animals were sacrificed at 4, 8, 12, and 15 weeks. There was an increasing amount of exposed root surface and root surface caries with age in the animals fed Diet 2000. There was exposed root surface but no caries in the animals fed laborato...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560091701
更新日期：1977-09-01 00:00:00
abstract：:The dental follicle (DF) differentiates into the periodontal ligament. In addition, it may be the precursor of other cells of the periodontium, including osteoblasts and cementoblasts. We hypothesized that stem cells may be present in the DF and be capable of differentiating into cells of the periodontium. Stem cells ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910808700801
更新日期：2008-08-01 00:00:00
abstract：:Current knowledge regarding the sensitivity of the teeth to forces is based on psychophysical experiments that measured touch detection thresholds under static jaw conditions. It is not known whether jaw movements alter the perception of forces applied to the teeth, but, based on limb movement studies, it is hypothesi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034510363101
更新日期：2010-04-01 00:00:00
abstract：:Teeth to be extracted for orthodontic reasons were filled in vivo with regular and fine-cut silver amalgams containing 1% and 5% SnF2. The teeth were extracted after 15 or 30 d, and the in vivo fluoride release and uptake by the dental tissues were studied using an electron microprobe analysis technique. The obtained ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600020101
更新日期：1981-02-01 00:00:00
abstract：:Teeth undergo postnatal organogenesis relatively late in life and only complete full maturation a few years after the crown first erupts in the oral cavity. At this stage, development can be arrested if the tooth organ is damaged by either trauma or caries. Regenerative endodontic procedures (REPs) are a treatment alt...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034517693606
更新日期：2017-06-01 00:00:00
abstract：:The benefits of early orthodontic treatment are continuously discussed, but studies are few. We examined whether definite need for orthodontic treatment could be eliminated in public health care by systematically focusing on early intervention. One age cohort living in a rural Finnish municipality (N = 85) was regular...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910808700604
更新日期：2008-06-01 00:00:00
abstract：:Contact angle measurements on layers of bacteria were used to determine the bacterial surface free energy (gamma b) of a variety of oral streptococcal strains, both without and after being coated with human whole saliva. At least four isolates of each species, either freshly isolated or laboratory strains, were used. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345850640100501
更新日期：1985-10-01 00:00:00
abstract：:Lysozyme concentration was quantitated immunochemically in parotid and submandibular-sublingual saliva of 46 caries-resistant and 17 caries-susceptible adults. There was essentially no difference between the two groups. The concentration of lysozyme was three times higher in the submandibular-sublingual than in the pa...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345830620050701
更新日期：1983-05-01 00:00:00
abstract：:The adsorption isotherms of benzoic acid on synthetic hydroxyapatite (containing about 1.5 monolayers of physisorbed water) were studied from ethanol, dimethylsulfoxide, p-dioxane, methylene chloride, and benzene to discern the role of solvent in the process. The adsorption is reversible from the first three solvents ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650051601
更新日期：1986-05-01 00:00:00
abstract：:Using a pin-on-disc sliding-wear test apparatus, the wear behavior of a typical composite restorative vs. styli fashioned from human enamel, sintered alumina, and magnesium fluoride was examined. The rates of restorative wear were found to be identical, within the experimental error of the measurements, for tests usin...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345840630062501
更新日期：1984-06-01 00:00:00
abstract：:A silver-plating technique was developed in an effort to produce good mechanical bonding characteristics between stainless steel pins and amalgam. Metallographic microscope and scanning electron microscope (SEM) studies were made to assess the presence, or otherwise, of such a bond between (a) the silver layer plating...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345800590020301
更新日期：1980-02-01 00:00:00
abstract：:The aim of this study was to investigate the diffusion dynamics of 25% hydrogen peroxide (H2O2) through enamel-dentin layers and to correlate it with dentin's structural alterations. Micro-Raman Spectroscopy (MRS) and Fourier Transform Infrared Photoacoustic Spectroscopy (FTIR-PAS) were used to measure the spectra of ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034513488893
更新日期：2013-07-01 00:00:00
abstract：:The effects of Escherichia coli endotoxin upon mouse L929 cell proliferation, DNA synthesis, protein synthesis, and proline incorporation were determined. It was found that a level of endotoxin which inhibited cell proliferation prompted a similar inhibition of DNA synthesis and overall cell protein synthesis. In cont...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580061301
更新日期：1979-06-01 00:00:00
abstract：:Current challenges in adhesive dentistry include over-hydrophilic bonding formulations, which facilitate water percolation through the hybrid layer and result in unreliable bonded interfaces. This study introduces nanogel-modified adhesives as a way to control the material's hydrophobic character without changing the ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034511426573
更新日期：2012-02-01 00:00:00
abstract：:Carbamide peroxide (CP) is widely used as a tooth-whitening agent in self-administered tooth-bleaching products. In this study, the effects of 5% and 10% CP on dentinal collagen structure and chemical properties were evaluated in vitro. Thirty-five intact teeth were exposed to 2 whitening protocols (2 or 4 h daily) wi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034518822826
更新日期：2019-04-01 00:00:00
abstract：:A major extracellular protein of cultured adult human gingival fibroblasts was shown to be fibronectin by indirect immunofluorescence, lactoperoxidase catalyzed iodination and metabolic radiolabeling. Fibronectin preparations from these cells possessed the biological properties of agglutination of sheep red blood cell...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345800590031301
更新日期：1980-03-01 00:00:00
abstract：:Adhesive bonding of resins to dentin surfaces requires the removal of the layer of debris caused by the cutting. Certain isotonic acidic solutions can do this rapidly. Five solutions were evaluated using cell cultures and pulp studies in monkeys. At concentrations above 10%, each solution caused the cultured human epi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345820610081001
更新日期：1982-08-01 00:00:00
abstract：:The histopathologic status of gingival tissue was assessed according to the criteria of Page and Schroeder.(5) Rhodamine-labeled bacteria were used to assess the antigenic specificity of plasma cells in the tissue. Examination of tissue sections revealed that 31.6% of plasma cells in the advanced lesions bound the lab...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034580059S213
更新日期：1980-11-01 00:00:00
abstract：:The temporomandibular joint (TMJ) undergoes degenerative changes among patients who suffer from arthritis, and yet the pathogenesis of TMJ osteoarthritis and rheumatoid arthritis is poorly understood. We hypothesized that sustained inflammation in the TMJ induces structural abnormalities, and accordingly characterized...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034512441946
更新日期：2012-05-01 00:00:00
abstract：:A previously reported technique for staining areas of degradation in dental composite restorations was evaluated in 51 removed restorations. The staining reagent was silver nitrate, which penetrated the degraded subsurface as ionic silver and was subsequently developed into colored deposits of metallic silver. Several...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345910700031201
更新日期：1991-03-01 00:00:00
abstract：:The effect of a topical fluoride regimen consisting of a four-minute pre-treatment with a saturated dicalcium phosphate dihydrate (DCPD) solution followed by a four-minute application of APF was compared with that of a four-minute application of APF alone with respect to the deposition of fluoride in enamel in an apat...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650030201
更新日期：1986-03-01 00:00:00
abstract：:Streptococcus mutans produces 3 types of glucosyltransferase (GTF), whose cooperative action is considered to be essential for its cellular adherence to the tooth surface. However, the precise mechanisms for synthesizing adhesive glucans and the specific roles of each GTF in cellular adherence to smooth surfaces have ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345010800071401
更新日期：2001-07-01 00:00:00
abstract：:Mutations in ALPL result in hypophosphatasia (HPP), a disease causing defective skeletal mineralization. ALPL encodes tissue nonspecific alkaline phosphatase (ALP), an enzyme that promotes mineralization by reducing inorganic pyrophosphate, a mineralization inhibitor. In addition to skeletal defects, HPP causes dental...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034515573273
更新日期：2015-05-01 00:00:00
abstract：:Since 1935, various mechanisms have been suggested for the formation of subsurface lesions and, in particular, the surface layer covering enamel lesions. The relatively intact mineral-rich and porous surface layer is most likely caused by kinetic events. The suggested mineral-rich outer layer in sound enamel, the orga...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/00220345860650010201
更新日期：1986-01-01 00:00:00
abstract：:The purpose of this study was to investigate the mechanism of surface layer formation in artificial white spots. Two nondestructive nuclear micro-analysis techniques were employed to analyze windows of human dental enamel at progressive stages of an artificial lesion. Fluoride concentrations were measured to a depth o...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345830620091001
更新日期：1983-09-01 00:00:00
abstract：:Ten dental casting alloys were tested for alloy-element release into cell-culture medium, and this release was related to alloy composition, alloy microstructure, and alloy cytotoxicity (previously determined). Cell-culture medium was analyzed for alloy elements by flame atomic absorption. Concentrations of elements i...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345910700060301
更新日期：1991-06-01 00:00:00
abstract：:An acidified gel system was used to produce artificial caries lesions in root surfaces. Radiopaque surface layers were found with 47% of the lesions. Demineralized bodies of the lesion and mineralized zones of banding were seen in all lesions when examined by microradiography. Cementum and dentin seemed to respond to ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345850640050401
更新日期：1985-05-01 00:00:00
