Abstract：
:Perinatal hypoxic ischemic (HI) brain injury is a leading cause of long-term neurological handicap in newborn babies. Recently, excessive activity of matrix metalloproteinases (MMPs), and in particular MMP-9, has been implicated in the aetiology of HI injuries to the immature brain. Our previous study suggested that MMP-9 may be involved in the development of the delayed injury processes following HI injury to the developing brain. Given this, we therefore propose that MMP-9 may be a useful target for rescue therapies in the injured developing brain. To address this, we chose to use SB-3CT, a highly selective inhibitor that is known to target only MMP-2 and MMP-9, to attenuate the elevated MMP-9 activity seen following HI injury to the developing brain. Twenty-one-day-old postnatal Wistar rats were subjected to unilateral carotid artery occlusion followed by exposure to hypoxia (8% oxygen for 1 h). SB-3CT (50 mg/kg body weight in 25% dimethyl sulphoxide/75% polyethylene glycol) or an equal volume of vehicle or saline diluent was then administered intraperitoneally at 2, 5 and 14 h following the insult. Gelatin zymography revealed that pro-MMP-9 levels were significantly reduced at 6 h following hypoxic ischaemia (p ≤ 0.05). However, our results showed that despite significantly inhibiting brain pro-MMP-9 activity after hypoxic ischaemia, SB-3CT failed to confer significant neuroprotection in postnatal day 21 rats 3 days after an HI insult. Further investigations are warranted using a recently reported selective water-soluble version of SB-3CT or another MMP-9 selective inhibitor to resolve the role of MMP-9 in the aetiology of HI injury in the developing brain.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Ranasinghe HS,Scheepens A,Sirimanne E,Mitchell MD,Williams CE,Fraser Mdoi
10.1159/000343257subject
Has Abstractpub_date
2012-01-01 00:00:00pages
417-27issue
5eissn
0378-5866issn
1421-9859pii
000343257journal_volume
34pub_type
杂志文章abstract：:α-Synuclein is one of the most abundant proteins in presynaptic terminals. Normal expression of α-synuclein is essential for neuronal survival and it prevents the initiation of apoptosis in neurons through covalent cross-linking of cytochrome c released from mitochondria. Exocytosis of α-synuclein occurs with neuronal...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000321342
更新日期：2010-01-01 00:00:00
abstract：:Cortex cerebri pieces from fetal donors were homologously and bilaterally grafted to the anterior eye chamber of adult albino rats. After intraocular maturation, presence of an external glial membrane, glial membranes surrounding blood vessels and cortical astrocytes was demonstrated using antisera raised against glia...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112703
更新日期：1982-01-01 00:00:00
abstract：:Dissociated brain cell cultures are a useful model for investigating development and differentiation of oligodendrocytes in vitro. The current studies compare the developmental patterns of expression for oligodendrocyte lineage/myelin markers in both primary and secondary oligodendrocyte cultures derived from mouse an...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000111276
更新日期：1995-01-01 00:00:00
abstract：:Birth asphyxia can result in sensory impairment, learning and memory deficits without gross brain injury and severe motor deficits. We developed a model of birth asphyxia resulting in mild neurological injury and cognitive impairment using a long-gestation species with precocial fetal development. Spiny mice (Acomys c...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000251907
更新日期：2009-01-01 00:00:00
abstract：:Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellum extracts analyzed by two-dimensional gels, using antipeptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mib-CK) and revealed the presence o...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000111452
更新日期：1996-01-01 00:00:00
abstract：:Several studies have shown that exogenous gangliosides can stimulate neurite outgrowth (in vitro), accelerate peripheral nerve regeneration (in vivo) and facilitate CNS recovery after lesioning. Experiments were designed to assess the effects of ganglioside administration on neonatal development. Rat neonates received...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112339
更新日期：1983-01-01 00:00:00
abstract：:Adult neuronal ceroid lipofuscinosis (NCL), also called Kufs' disease, is clinically distinct from the other NCLs. It is a rare condition which is difficult to diagnose. More than 50% of the reported cases of Kufs' disease are not adult NCL and correspond very likely to a heterogeneous spectrum of lipidoses. Various c...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000112182
更新日期：1991-01-01 00:00:00
abstract：:We studied the developmental changes of soluble and membrane-bound protein kinases (EC 2.7.1.37) in rat brain and found that the elution profiles from a DEAE-cellulose column by NaCl of adenosine-3':5'-monophosphate (cyclic AMP) dependent protein kinase from cytosol were not significantly different in newborn (0.19 M ...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112450
更新日期：1979-01-01 00:00:00
abstract：:The myelin-associated glycoprotein (MAG) in primary cultures of oligodendrocytes is subject to phosphorylation in the absence of neurons. The positive response of this phosphorylation to vanadate suggests that one of the modified sites in MAG is phosphotyrosine. We found that phosphorylation was enhanced by brief trea...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112192
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND:Neonatal hypoxic-ischemic (HI) encephalopathy occurs in 1-4 per 1,000 live term births and can cause devastating neurodevelopmental disabilities. Currently, therapeutic hypothermia (TH) is the only treatment with proven efficacy. Since TH is associated with decreased cerebral metabolism and cerebral blood fl...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000381704
更新日期：2015-01-01 00:00:00
abstract：:Nogo is a member of the reticulon family. Our understanding of the physiological functions of the Nogo-A protein has grown over the last few years, and this molecule is now recognized as one of the most important axonal regrowth inhibitors present in central nervous system (CNS) myelin. Nogo-A plays other important ro...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000343143
更新日期：2012-01-01 00:00:00
abstract：:We report on the effects of etiology and age on the linguistic outcomes in a large pediatric hemispherectomy population. Four populations were considered separately: cortical dysplasia (multilobar involvement), Rasmussen's encephalitis, infarction as a primary etiology and, fourth, children who failed to develop langu...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000017396
更新日期：1999-11-01 00:00:00
abstract：:Cell polarity is one of the most basic properties of all normal cells and loss of polarity is a hallmark of cancer. While multiple proteins have been implicated in the maintenance of cell polarity, the functionally related neoplastic tumor suppressors Lethal giant larvae (Lgl), Scribble and Disks large comprise a uniq...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000090749
更新日期：2006-01-01 00:00:00
abstract：:Proper eye formation depends on specific interactions between neural and ectodermal tissues coupled with temporally distinct gene expression and a regulated sequence of signaling events. The homeobox gene Pax6 is vitally important to the entire process of eye development in both vertebrates and invertebrates. Pax6 exp...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000082273
更新日期：2004-01-01 00:00:00
abstract：:The lipids of white matter and peripheral nerve from mutant mice with known myelin deficiencies were analyzed by one- and two-dimensional high-performance thin-layer chromatography and quantitated by densitometry. In optic nerve, the mutants jp/Y, jpmsd/Y, qk/qk, shi/shi and shimld/shimld, which have severe central ne...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000111961
更新日期：1988-01-01 00:00:00
abstract：:This study analyzed the role of dopamine D1 and D2 receptors in methylphenidate (MPH) conditioned place preference (CPP) in adolescent male and female rats, in addition to the role of these receptors in the effects of MPH on brain-derived neurotrophic factor (BDNF) in the dorsal striatum and nucleus accumbens. Using a...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000360636
更新日期：2014-01-01 00:00:00
abstract：:Striatal neurons are highly vulnerable to hypoxia-ischemia (HI) in term newborns. In a piglet model of HI, striatal neurons develop oxidative stress and organelle disruption by 3-6 h of recovery and ischemic cytopathology over 6-24 h of recovery. We tested the hypothesis that early treatment with the antioxidants EUK-...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000327243
更新日期：2011-01-01 00:00:00
abstract：:Schizophrenia is a chronic and debilitating mental illness characterized by a broad range of abnormal behaviors, including delusions and hallucinations, impaired cognitive function, as well as mood disturbances and social withdrawal. Due to the heterogeneous nature of the disease, the causes of schizophrenia are very ...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000346157
更新日期：2013-01-01 00:00:00
abstract：:Perinatal asphyxia is a leading cause of brain injury in neonates, occurring in 2-4 per 1,000 live births, and there are limited treatment options. Because of their similarity to humans, nonhuman primates are ideal for performing preclinical tests of safety and efficacy for neurotherapeutic interventions. We previousl...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000327246
更新日期：2011-01-01 00:00:00
abstract：:We have previously shown that a single intracranial injection of apotransferrin (aTf) in neonatal rats produces an accelerated mylinogenesis and increases the expression of certain myelin proteins such as myelin basic protein (MBP). In the present work, we studied the effects of aTf upon oligodendrocyte progenitor cel...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000064945
更新日期：2002-01-01 00:00:00
abstract：:HIV-1 is able to penetrate the developing human central nervous system and induce damage. Clinical and neuropathological observations demonstrate that (a) the virus is transmitted during the late phase of pregnancy or at the time of delivery; (b) the main infected cells within the brain are macrophages, and (c) the me...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000112100
更新日期：1994-01-01 00:00:00
abstract：:We have previously provided evidence that ferritin binds selectively to white matter tracts in adult mouse and human brains. In cell culture experiments, ferritin binding is specifically localized to oligodendrocytes. The goal of the present study is to test the hypothesis that the developmental pattern for ferritin b...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000065704
更新日期：2002-01-01 00:00:00
abstract：:Stromal-derived factor 1 (SDF-1), a known chemoattractant, and its receptor CXCR4 are widely expressed in the developing and adult cerebral cortex. Recent studies have highlighted potential roles for SDF-1 during early cortical development. In view of the current findings, our histological analysis has revealed a dist...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000109857
更新日期：2008-01-01 00:00:00
abstract：:Our previous studies of H218, a sphingosine 1-phosphate (S1P) receptor and a member of the G-protein-coupled receptor superfamily, suggest that it may participate in mammalian nervous system development. Thus, brain levels of H218 mRNA are higher during early neurogenesis than postnatally. In addition, embryonic H218 ...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000017452
更新日期：2000-01-01 00:00:00
abstract：:Preadolescent animals display protracted hormonal stress responses mediated by the hypothalamic-pituitary-adrenal (HPA) axis compared to adults. Though the mechanisms that underlie this shift in stress reactivity are unknown, reduced glucocorticoid-dependent negative feedback on the HPA axis has been posited to contri...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000362873
更新日期：2014-01-01 00:00:00
abstract：:The distal segments of the crush-injured and permanently transected sciatic nerve provide models to study Schwann cell activity in the presence and absence of Schwann cell-axonal contact, respectively. We examined the quantity and quality of transcript coding for the myelin-associated glycoprotein (MAG) over a 3-week ...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000111832
更新日期：1990-01-01 00:00:00
abstract：:Three experimental approaches were used to examine the maturation of N-methyl-D-aspartate (NMDA) receptors in the neostriatum and compare their developmental profile to that of the non-NMDA receptors [alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate (KA)]. The first, and least conventional ...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000017310
更新日期：1998-01-01 00:00:00
abstract：:We have used whole-cell and single-channel recording techniques to investigate the electrophysiological properties of cultured ovine oligodendrocytes (OLGs). Our studies have led to the following conclusions. (1) Cultured mature OLGs express a variety of voltage-dependent K+ conductances including an outward current t...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000111893
更新日期：1989-01-01 00:00:00
abstract：:The long noncoding RNA MSNP1AS (moesin pseudogene 1, antisense) is a functional element that was previously associated with autism spectrum disorder (ASD) with genome-wide significance. Expression of MSNP1AS was increased 12-fold in the cerebral cortex of individuals with ASD and 22-fold in individuals with a genome-w...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000453258
更新日期：2016-01-01 00:00:00
abstract：:Glutamine synthesis, the major pathway of ammonia detoxification, and the intracellular concentration of organic osmolytes in primary astrocytes and F98 glioma cells were investigated with multinuclear magnetic resonance spectroscopy. Acute exposure to ammonia (3 h incubation with NH4Cl) raised the concentration of gl...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000017339
更新日期：1998-01-01 00:00:00
