Abstract:
:Dissociated brain cell cultures are a useful model for investigating development and differentiation of oligodendrocytes in vitro. The current studies compare the developmental patterns of expression for oligodendrocyte lineage/myelin markers in both primary and secondary oligodendrocyte cultures derived from mouse and rat neonates. The rat and mouse dissociated brain cell cultures express the same myelin-specific antigens, but mouse oligodendrocytes produce a larger and more elaborate sheet-like membrane than rat oligodendrocytes, and some of the myelin markers (O4, GC, and MBP) show more intense membrane staining in mouse cultures. GD3 appears to be a good oligodendrocyte marker for rat cells, but it is nonspecific in mouse cells. There are fewer oligodendrocytes in mouse cultures, and they appear to require a longer differentiation time than rat oligodendrocytes. These same results are also observed in secondary oligodendrocyte cultures, although in general late myelin markers such as MBP and PLP are expressed at a much lower level in mouse cells than rat cells.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Duchala CS,Asotra K,Macklin WBdoi
10.1159/000111276subject
Has Abstractpub_date
1995-01-01 00:00:00pages
70-80issue
2eissn
0378-5866issn
1421-9859journal_volume
17pub_type
杂志文章abstract::Choline acetyltransferase (ChAT), the biosynthetic enzyme for acetylcholine metabolism, is expressed in a human cholinergic neuroblastoma cell line; MC-IXC. We demonstrate that ChAT activity is regulated in this cell line by cell density. It is believed that the mechanism of stimulation of enzyme activity involves cel...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111975
更新日期:1988-01-01 00:00:00
abstract::Metabolism of glutamate and glutamine in cultured mouse cerebral cortical astrocytes has been investigated using either radioactively labelled (14C) amino acids or 13C-labelled amino acids combined with NMR spectroscopy of cell extracts and lyophilyzed incubation media. Using [U-13C]glutamate it has been shown that in...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111356
更新日期:1993-01-01 00:00:00
abstract::The distal segments of the crush-injured and permanently transected sciatic nerve provide models to study Schwann cell activity in the presence and absence of Schwann cell-axonal contact, respectively. We examined the quantity and quality of transcript coding for the myelin-associated glycoprotein (MAG) over a 3-week ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111832
更新日期:1990-01-01 00:00:00
abstract::Prenatal stress has been associated with detrimental outcomes of pregnancy, including altered brain development leading to behavioural pathologies. The neurosteroid allopregnanolone has been implicated in mediating some of these adverse outcomes following prenatal stress due to its potent inhibitory and anxiolytic eff...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000437302
更新日期:2015-01-01 00:00:00
abstract::The distribution of the noradrenergic system within the rat cerebellar cortex following low-level X-irradiation was studied using tyrosine hydroxylase immunocytochemistry. X-Irradiation treatments consisted of 1, 3, or 5 successive daily dose beginning on postnatal day 1. When studied 24 h after the last exposure, a d...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112092
更新日期:1994-01-01 00:00:00
abstract::In this study we have examined the susceptibility of the mitochondrial respiratory chain of astrocytes and astrocytes depleted of glutathione to peroxynitrite exposure. Astrocytes, as reported previously by us, appeared resistant to the actions of peroxynitrite. In contrast, depletion (-94%) of astrocytic glutathione ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111432
更新日期:1996-01-01 00:00:00
abstract::The M50 and M100 auditory evoked responses reflect early auditory processes in the primary/secondary auditory cortex. Although previous M50 and M100 studies have been conducted on individuals with autism spectrum disorder (ASD) and indicate disruption of encoding simple sensory information, analogous investigations of...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000504960
更新日期:2019-01-01 00:00:00
abstract::Ethanol inhibition of L1-mediated cell adhesion may contribute to the spectrum of neurological, behavioral and morphological abnormalities associated with prenatal ethanol exposure. We showed previously that the neuroprotective peptides NAPVSIPQ (NAP) and SALLRSIPA (SAL) antagonize ethanol inhibition of L1 adhesion an...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000084528
更新日期:2005-01-01 00:00:00
abstract::The cell line MN9D, a fusion of embryonic ventral mesencephalic and neuroblastoma cells, is extensively used as a model of dopamine (DA) neurons because it expresses tyrosine hydroxylase and synthesizes and releases DA. These cells are also used to test mechanisms and potential therapeutics relevant to the loss of DA ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000095115
更新日期:2006-01-01 00:00:00
abstract::Recent studies have shown a protection from cerebral hypoxic-ischemic (HI) brain damage in the immature rat following a prior systemic hypoxic exposure when compared with those not exposed previously. To investigate the mechanism(s) of hypoxic preconditioning, brain glycogen and high-energy phosphate reserves were mea...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000069051
更新日期:2002-01-01 00:00:00
abstract::Nitric oxide may serve as a retrograde messenger to refine or stabilize synapses in the developing nervous system. Whether this action is dependent upon glutamate and the N-methyl-D-aspartate receptor is not yet established. We have used the patch-cluster system in the intermediate gray layer (IGL) of the rat superior...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111215
更新日期:1997-01-01 00:00:00
abstract::The neonatal brain appears to be selectively vulnerable to oxidative stress. Several potential mechanisms associated with altered reactive oxygen species metabolism would explain the increased susceptibility. They include increased accumulation of hydrogen peroxide with subsequent neurotoxicity. This enhanced neurotox...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000046143
更新日期:2001-01-01 00:00:00
abstract::The long noncoding RNA MSNP1AS (moesin pseudogene 1, antisense) is a functional element that was previously associated with autism spectrum disorder (ASD) with genome-wide significance. Expression of MSNP1AS was increased 12-fold in the cerebral cortex of individuals with ASD and 22-fold in individuals with a genome-w...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000453258
更新日期:2016-01-01 00:00:00
abstract::Using Western blot and immunocytochemistry to detect protein, and in situ hybridization and Northern blot to detect RNA, we studied the expression of Na,K-ATPase subunit isoforms in cultured astrocytes and oligodendrocytes in vitro, and in glial cells in the brain. We found that astrocytes in culture express alpha 1 a...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111422
更新日期:1996-01-01 00:00:00
abstract::In the socially monogamous prairie vole, we have observed that small changes in early handling, as well as early hormonal manipulations can have long-lasting and sexually dimorphic effects on behavior. These changes may be mediated in part by changes in parental interactions with their young, acting on systems that re...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000216544
更新日期:2009-01-01 00:00:00
abstract::We have previously shown that lipopolysaccharide (LPS) sensitizes the immature rat brain to subsequent hypoxic-ischemic (HI) injury; however, the underlying mechanisms remain unclear. In this study, we examined the role of glucose in the sensitizing effects of LPS and lipoteichoic acid (LTA) in combination with HI in ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000080713
更新日期:2004-01-01 00:00:00
abstract::Parallel developmental studies of central nervous system myelin proteins and morphology (postnatal days 15-118; P15-118) confirm qualitative similarities but substantial quantitative differences between homozygous mld mice with Billings-Gagliardi and Wolf's 'USA' versus Matthieu's 'Swiss' genetic backgrounds. The USA ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112145
更新日期:1991-01-01 00:00:00
abstract::L-Ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine biosynthesis and thus a unique marker of the proliferation and maturation processes, has been localized by means of immunohistochemistry in the human central nervous system. The enzyme protein has not only been found in the developing nervous syste...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111320
更新日期:1993-01-01 00:00:00
abstract::Apoptosis is a controlled form of cell death that participates in the demise of neuronal cells during development, neurodegenerative disorders and exposure to neurotoxic agents. In recent years, the mitochondria have emerged as being pivotal in controlling apoptosis. They house a number of apoptogenic molecules that a...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000017460
更新日期:2000-09-01 00:00:00
abstract::It has been hypothesized that changes in striatal-mediated dopamine modulation during adolescence may increase the risk for initiating substance abuse as a result of its fundamental role in arbitrating reward sensitivity and motivation during learning and decision making. However, substance abuse during adolescence ma...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000337724
更新日期:2012-01-01 00:00:00
abstract::Neuroprotective cold-shock proteins (CSPs) are abundant in the normothermic neonatal rodent brain but decrease with advancing neurodevelopmental age and are low or absent in the adult brain. It has not been established if neurodevelopmental age alters the baseline expression of CSPs in the human brain. Here, we tested...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000493637
更新日期:2018-01-01 00:00:00
abstract::The myelin-associated glycoprotein (MAG) in primary cultures of oligodendrocytes is subject to phosphorylation in the absence of neurons. The positive response of this phosphorylation to vanadate suggests that one of the modified sites in MAG is phosphotyrosine. We found that phosphorylation was enhanced by brief trea...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112192
更新日期:1991-01-01 00:00:00
abstract::Neurotransmitters play important roles in neurogenesis; in particular, acetylcholine and serotonin may regulate neurite elongation. Acetylcholine may also activate transcription factors such as early growth response protein 1 (EGR-1), which plays a role in neurite extension. N18TG2 neuroblastoma cells (which do not pr...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000354423
更新日期:2013-01-01 00:00:00
abstract::Dissociated cells of cerebral hemispheres from 8-day-old chick embryos were cultivated for 8 days in polylysine-coated Petri dishes. Changes of DNA, RNA, total proteins, putrescine, spermidine, spermine, free amino acids and enzymes involved in polyamine and GABA metabolism were studied throughout neuronal development...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112755
更新日期:1981-01-01 00:00:00
abstract::We have used whole-cell and single-channel recording techniques to investigate the electrophysiological properties of cultured ovine oligodendrocytes (OLGs). Our studies have led to the following conclusions. (1) Cultured mature OLGs express a variety of voltage-dependent K+ conductances including an outward current t...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111893
更新日期:1989-01-01 00:00:00
abstract::Three experimental approaches were used to examine the maturation of N-methyl-D-aspartate (NMDA) receptors in the neostriatum and compare their developmental profile to that of the non-NMDA receptors [alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and kainate (KA)]. The first, and least conventional ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017310
更新日期:1998-01-01 00:00:00
abstract::Early developmental treatment of rats with 3,4-methylenedioxymethamphetamine (MDMA) was previously found to cause an abnormal pattern of forebrain serotonergic axon density in adulthood consisting of a cortical hypoinnervation and a striatal hyperinnervation. The present study tested the hypothesis that this reorganiz...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000207497
更新日期:2009-01-01 00:00:00
abstract::As a step toward using two closely related members of the nuclear receptor family, RORalpha and RORbeta, as markers and tools for genetic manipulations in mouse forebrain, we have used in situ hybridization to analyze their expression from E10.5 to P7. At later embryonic and early postnatal ages, RORalpha expression i...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000072271
更新日期:2003-03-01 00:00:00
abstract::Prenatal cocaine exposure results in behavioral abnormalities throughout development in rats, but little is known regarding the biological mechanisms underlying these abnormalities. Pregnant rats received subcutaneous twice-daily injections (1 ml/kg) of normal saline or 15 mg/kg of cocaine hydrochloride throughout ges...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000069363
更新日期:2002-01-01 00:00:00
abstract::Protein synthetic activity of anterior hypothalamic and neocortical brain slices from androgenized neonatal female rats was studied in vitro in an attempt to clarify the mechanism of action of testosterone on the developing hypothalamus. The short-term effects of testosterone propionate injections of the animals on th...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112449
更新日期:1979-01-01 00:00:00