Abstract:
:Ethanol inhibition of L1-mediated cell adhesion may contribute to the spectrum of neurological, behavioral and morphological abnormalities associated with prenatal ethanol exposure. We showed previously that the neuroprotective peptides NAPVSIPQ (NAP) and SALLRSIPA (SAL) antagonize ethanol inhibition of L1 adhesion and prevent ethanol-induced growth retardation in mouse whole embryo culture. Here we ask whether NAP and SAL also prevent ethanol-induced major malformations of the nervous system. Gestational day 8.0 (3-5 somites) C57BL/6J mouse embryos were grown for 6 h in control medium, 100 mM ethanol and 10(-10) M peptides and then maintained for an additional 20 h in control medium. At the end of the culture period, only embryos having 18-19 somite pairs were examined and compared for the degree of neural tube closure. Ethanol exposure resulted in neural tube defects (NTDs) consistent with total dysraphia and anencephaly. Co-incubation with ethanol and L-NAP (all L-amino acids), D-NAP (all D-amino acids) or SAL significantly increased the percentage of embryos that had begun to close their neural folds at the level of the forebrain/midbrain junction or that had progressed beyond this stage of closure. P7A-NAP (NAPVSIAQ), which lacks neuroprotective activity, but retains activity as an antagonist of ethanol inhibition of L1 adhesion, was effective in preventing ethanol-induced NTDs. In contrast, I6A-NAP (NAPVSAPQ), which shows reduced efficacy as an ethanol antagonist but retains its neuroprotective efficacy, did not significantly diminish the induction of NTDs by ethanol. These findings demonstrate the ability of NAP and SAL to prevent ethanol-induced NTDs and support the hypothesis that ethanol teratogenesis is caused in part by ethanol inhibition of L1-mediated cell adhesion.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Chen SY,Charness ME,Wilkemeyer MF,Sulik KKdoi
10.1159/000084528keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
13-9issue
1eissn
0378-5866issn
1421-9859pii
84528journal_volume
27pub_type
杂志文章abstract::We have previously reported that a developmental axon-sparing striatal injury induced by hypoxia-ischemia results in a diminished adult number of substantia nigra (SN) tyrosine hydroxylase (TH)-positive neurons, in the absence of direct nigral injury. To examine the specific role of striatal injury, we made selective ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111684
更新日期:1992-01-01 00:00:00
abstract::Proper eye formation depends on specific interactions between neural and ectodermal tissues coupled with temporally distinct gene expression and a regulated sequence of signaling events. The homeobox gene Pax6 is vitally important to the entire process of eye development in both vertebrates and invertebrates. Pax6 exp...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000082273
更新日期:2004-01-01 00:00:00
abstract::The expression of voltage-gated potassium channels plays an important role in the acquisition of membrane excitability in neurons. We examined the expression pattern of genes in developing cerebellar granule neurons in vivo and in vitro. In situ hybridization of Kv3.1 mRNA demonstrated that the gene was expressed at h...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017370
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journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111882
更新日期:1989-01-01 00:00:00
abstract::Prenatal cocaine exposure results in behavioral abnormalities throughout development in rats, but little is known regarding the biological mechanisms underlying these abnormalities. Pregnant rats received subcutaneous twice-daily injections (1 ml/kg) of normal saline or 15 mg/kg of cocaine hydrochloride throughout ges...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000069363
更新日期:2002-01-01 00:00:00
abstract::Recent studies have shown a protection from cerebral hypoxic-ischemic (HI) brain damage in the immature rat following a prior systemic hypoxic exposure when compared with those not exposed previously. To investigate the mechanism(s) of hypoxic preconditioning, brain glycogen and high-energy phosphate reserves were mea...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000069051
更新日期:2002-01-01 00:00:00
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journal_title:Developmental neuroscience
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doi:10.1159/000327246
更新日期:2011-01-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000343143
更新日期:2012-01-01 00:00:00
abstract::In cultured glial cells from chick embryonic brain, both influx and efflux of 42K+ and 22Na+ are dependent on the external Ca++ and concentration ([Ca++]0) between 2 and 0.1 mM although intracellular concentrations of K+ ([K+]i) and Na+ ([Na+]i) do not change. Only a reduction of [Ca++]0 below 0.1 mM results in both a...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112664
更新日期:1982-01-01 00:00:00
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journal_title:Developmental neuroscience
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doi:10.1159/000066747
更新日期:2002-01-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000353820
更新日期:2013-01-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000437302
更新日期:2015-01-01 00:00:00
abstract::In vertebrate species, the neuropeptide oxytocin (OT) has been implicated in neural and behavioral development. Although several OT-like peptides have been characterized in invertebrate species, the ontogenesis of the OT-like system has not yet been described in these species. Thus, the aim of the present study was to...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000235942
更新日期:2010-03-01 00:00:00
abstract::Maternal peptides from the hypothalamic-pituitary-adrenal (HPA) axis rise during human pregnancy. The effects of circulating maternal adrenocorticotropin (ACTH) and beta-endorphin (BE) on human fetal behavior was determined in 135 women during their 32nd week of gestation. Fetal behavior was measured by assessing hear...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000071467
更新日期:2003-01-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000085978
更新日期:2005-03-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000046143
更新日期:2001-01-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017329
更新日期:1998-01-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112242
更新日期:1986-01-01 00:00:00
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journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000452859
更新日期:2017-01-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Developmental neuroscience
pub_type: 杂志文章
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journal_title:Developmental neuroscience
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journal_title:Developmental neuroscience
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更新日期:1978-01-01 00:00:00
abstract::The myelin-associated glycoprotein (MAG) is a 100 K-dalton, integral membrane glycoprotein containing 30% carbohydrate that is in central and peripheral myelin sheaths. Recent research on its function during normal myelinogenesis and possible roles in the pathology of demyelinating diseases is reviewed. Emphasis is pl...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000112356
更新日期:1983-01-01 00:00:00
abstract::We studied the developmental changes of soluble and membrane-bound protein kinases (EC 2.7.1.37) in rat brain and found that the elution profiles from a DEAE-cellulose column by NaCl of adenosine-3':5'-monophosphate (cyclic AMP) dependent protein kinase from cytosol were not significantly different in newborn (0.19 M ...
journal_title:Developmental neuroscience
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doi:10.1159/000112450
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journal_title:Developmental neuroscience
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pub_type: 杂志文章,评审
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