当前位置： SCI文献检索 > DEVELOPMENTAL NEUROSCIENCE期刊下所有文献 > A survey of neurological mutant mice. I. Lipid composition of myelinated tissue in known myelin mutants.

A survey of neurological mutant mice. I. Lipid composition of myelinated tissue in known myelin mutants.

Abstract：

:The lipids of white matter and peripheral nerve from mutant mice with known myelin deficiencies were analyzed by one- and two-dimensional high-performance thin-layer chromatography and quantitated by densitometry. In optic nerve, the mutants jp/Y, jpmsd/Y, qk/qk, shi/shi and shimld/shimld, which have severe central nervous system (CNS) myelin deficiency, had a common pattern of lipid loss: cerebrosides and sulfatides (hydroxy and nonhydroxy forms) were generally reduced by 70-95% or more; most phospholipids were diminished by 15-55%, and cholesterol was reduced by 35-60%. Only in the CNS of jp/Y and jpmsd/Y did cholesterol ester accumulate. In peripheral nerve, the lipid composition varied markedly among these mutants. In jp/Y there was no change, while in jpmsd/Y there was a 5-15% loss among the phospholipids and cholesterol. Homozygous qk had reductions of 75-85% in the nonhydroxy forms of cerebroside and sulfatide, a 130% increase in hydroxy sulfatide, and a 55% loss of sphingomyelin. In shi/shi and shimld/shimld homozygotes, the glycolipids were altered by +/- 20%, most phospholipids and cholesterol were reduced by 5-15%, and sphingomyelin was reduced by 40%. Tr and TrJ showed 35-90% reductions in most lipid classes of the peripheral nervous system; CNS lipid composition was normal. Homozygous twi had a uniform loss of most lipid classes in both optic (generally 10-20%) and trigeminal nerves (generally 40-55%); cerebrosides did not accumulate in these tissues. dy/dy had a 10-20% reduction of cerebrosides in trigeminal nerve trunk. The CNS of dy homozygotes had 10-35% increases in specific classes of glycolipids and phospholipids, and in cholesterol. None of the mutants showed detectable levels of lysophospholipids or other unusual lipid species. The fractions of ethanolamine and choline phosphatides in the plasmalogen form were close to normal in all mutants.

journal_name

Dev Neurosci

journal_title

Developmental neuroscience

authors

Ganser AL,Kerner AL,Brown BJ,Davisson MT,Kirschner DA

doi

10.1159/000111961

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

99-122

issue

eissn

0378-5866

issn

1421-9859

journal_volume

pub_type

杂志文章

在线工具

A survey of neurological mutant mice. I. Lipid composition of myelinated tissue in known myelin mutants.