Initial attachment of human oral keratinocytes cultured on zirconia or titanium.

Abstract:

:Initial attachment of human oral keratinocytes (HOKs) cultured on mirror-surfaced commercially pure titanium (Ti) or yttria-stabilized tetragonal zirconia polycrystals (TZP) was investigated. Numbers of viable attached HOKs, their mRNAs and proteins expression of laminin γ2 and integrin β4 were evaluated using the WST-1 assay, quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Localization of laminin γ2 and integrin β4 was observed using immunofluorescent staining. Cell spreading was evaluated by measuring the perimeter of actin on fluorescent stained images, and cell morphology was examined using scanning electron microscopy. At 1 h TZP elicited less of initial attachment than Ti in terms of mRNAs expression and proteins expression of laminin γ2 and integrin β4 (p<0.05). However, at 48 h TZP was showed similar initial attachment in comparison to Ti. Therefore, it was suggested that TZP has a potential to form epithelial attachment like Ti.

journal_name

Dent Mater J

journal_title

Dental materials journal

authors

Kimura Y,Matsuzaka K,Yoshinari M,Inoue T

doi

10.4012/dmj.2011-189

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

346-53

issue

3

eissn

0287-4547

issn

1881-1361

pii

DN/JST.JSTAGE/dmj/2011-189

journal_volume

31

pub_type

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