当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Export and stability of naturally intronless mRNAs require specific coding region sequences and the TREX mRNA export complex.

Export and stability of naturally intronless mRNAs require specific coding region sequences and the TREX mRNA export complex.

Abstract：

:A great deal is known about the export of spliced mRNAs, but little is known about the export of mRNAs encoded by human cellular genes that naturally lack introns. Here, we investigated the requirements for export of three naturally intronless mRNAs (HSPB3, IFN-α1, and IFN-β1). Significantly, we found that all three mRNAs are stable and accumulate in the cytoplasm, whereas size-matched random RNAs are unstable and detected only in the nucleus. A portion of the coding region confers this stability and cytoplasmic localization on the naturally intronless mRNAs and a cDNA transcript, which is normally retained in the nucleus and degraded. A polyadenylation signal, TREX mRNA export components, and the mRNA export receptor TAP are required for accumulation of the naturally intronless mRNAs in the cytoplasm. We conclude that naturally intronless mRNAs contain specific sequences that result in efficient packaging into the TREX mRNA export complex, thereby supplanting the splicing requirement for efficient mRNA export.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Lei H,Dias AP,Reed R

doi

10.1073/pnas.1113076108

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

17985-90

issue

44

eissn

0027-8424

issn

1091-6490

pii

1113076108

journal_volume

108

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Isolation of an ovine pulmonary surfactant-associated anionic peptide bactericidal for Pasteurella haemolytica.

    abstract：:Ovine pulmonary surfactant is bactericidal for Pasteurella haemolytica when surfactant and bacteria mixtures are incubated with normal ovine serum. To isolate this component, surfactant (1 mg/ml) was centrifuged at 100,000 x gav, and the supernatant was fractionated by HPLC. Fractions were eluted with acetonitrile (10...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.1.412

    authors： Brogden KA,De Lucca AJ,Bland J,Elliott S

    更新日期：1996-01-09 00:00:00

  • Identification of mitochondrial proteins and some of their precursors in two-dimensional electrophoretic maps of human cells.

    abstract：:A set of at least 30 proteins disappears from the two-dimensional electrophoretic pattern of human lymphoid cells treated with various antimitochondrial agents. This set is similar to the set of proteins found in isolated mitochondria (except for the presence of action in the latter group), indicating that the inhibit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.4.2407

    authors： Anderson L

    更新日期：1981-04-01 00:00:00

  • Default-mode network activity distinguishes Alzheimer's disease from healthy aging: evidence from functional MRI.

    abstract：:Recent functional imaging studies have revealed coactivation in a distributed network of cortical regions that characterizes the resting state, or default mode, of the human brain. Among the brain regions implicated in this network, several, including the posterior cingulate cortex and inferior parietal lobes, have al...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0308627101

    authors： Greicius MD,Srivastava G,Reiss AL,Menon V

    更新日期：2004-03-30 00:00:00

  • A general assay for antibody catalysis using acridone as a fluorescent tag.

    abstract：:A simple and highly sensitive catalysis assay is demonstrated based on analyzing reactions with acridonetagged compounds by thin-layer chromatography. As little as 1 pmol of product is readily visualized by its blue fluorescence under UV illumination and identified by its retention factor (Rf). Each assay requires onl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.9.4251

    authors： Reymond JL,Koch T,Schröer J,Tierney E

    更新日期：1996-04-30 00:00:00

  • Microparadigms: chains of collective reasoning in publications about molecular interactions.

    abstract：:We analyzed a very large set of molecular interactions that had been derived automatically from biological texts. We found that published statements, regardless of their verity, tend to interfere with interpretation of the subsequent experiments and, therefore, can act as scientific "microparadigms," similar to domina...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0600591103

    authors： Rzhetsky A,Iossifov I,Loh JM,White KP

    更新日期：2006-03-28 00:00:00

  • Latent luciferase activity in the fruit fly revealed by a synthetic luciferin.

    abstract：:Beetle luciferases are thought to have evolved from fatty acyl-CoA synthetases present in all insects. Both classes of enzymes activate fatty acids with ATP to form acyl-adenylate intermediates, but only luciferases can activate and oxidize d-luciferin to emit light. Here we show that the Drosophila fatty acyl-CoA syn...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1319300111

    authors： Mofford DM,Reddy GR,Miller SC

    更新日期：2014-03-25 00:00:00

  • A trans-Golgi network golgin is required for the regulated secretion of TNF in activated macrophages in vivo.

    abstract：:The transmembrane precursor of tumor necrosis factor-alpha (TNF) exits the trans-Golgi network (TGN) in tubular carriers for subsequent trafficking and delivery to the cell surface; however, the molecular machinery responsible for Golgi export is unknown. We previously reported that members of the TGN golgin family ar...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0800137105

    authors： Lieu ZZ,Lock JG,Hammond LA,La Gruta NL,Stow JL,Gleeson PA

    更新日期：2008-03-04 00:00:00

  • During infant maltreatment, stress targets hippocampus, but stress with mother present targets amygdala and social behavior.

    abstract：:Infant maltreatment increases vulnerability to physical and mental disorders, yet specific mechanisms embedded within this complex infant experience that induce this vulnerability remain elusive. To define critical features of maltreatment-induced vulnerability, rat pups were reared from postnatal day 8 (PN8) with a m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1907170116

    authors： Raineki C,Opendak M,Sarro E,Showler A,Bui K,McEwen BS,Wilson DA,Sullivan RM

    更新日期：2019-11-05 00:00:00

  • Amyloid beta -protein (Abeta) assembly: Abeta 40 and Abeta 42 oligomerize through distinct pathways.

    abstract：:Amyloid beta-protein (Abeta) is linked to neuronal injury and death in Alzheimer's disease (AD). Of particular relevance for elucidating the role of Abeta in AD is new evidence that oligomeric forms of Abeta are potent neurotoxins that play a major role in neurodegeneration and the strong association of the 42-residue...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.222681699

    authors： Bitan G,Kirkitadze MD,Lomakin A,Vollers SS,Benedek GB,Teplow DB

    更新日期：2003-01-07 00:00:00

  • Changing resonator geometry to boost sound power decouples size and song frequency in a small insect.

    abstract：:Despite their small size, some insects, such as crickets, can produce high amplitude mating songs by rubbing their wings together. By exploiting structural resonance for sound radiation, crickets broadcast species-specific songs at a sharply tuned frequency. Such songs enhance the range of signal transmission, contain...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1200192109

    authors： Mhatre N,Montealegre-Z F,Balakrishnan R,Robert D

    更新日期：2012-05-29 00:00:00

  • Probing chelation motifs in HIV integrase inhibitors.

    abstract：:A series of HIV integrase (HIV-1 IN) inhibitors were synthesized to evaluate the role of the metal-binding group (MBG) in this class of metalloenzyme inhibitors. A total of 21 different raltegravir-chelator derivative (RCD) compounds were prepared that differed only in the nature of the MBG. These IN strand-transfer i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1112389109

    authors： Agrawal A,DeSoto J,Fullagar JL,Maddali K,Rostami S,Richman DD,Pommier Y,Cohen SM

    更新日期：2012-02-14 00:00:00

  • Structure of poly(adenosine diphosphate ribose): identification of 2'-[1''-ribosyl-2''-(or 3''-)(1'''-ribosyl)]adenosine-5',5'',5'''-tris(phosphate) as a branch linkage.

    abstract：:Poly([14C]adenosine diphosphate ribose) was synthesized from [14C]NAD+ with calf thymus nuclei. The fraction containing poly(adenosine diphosphate ribose) eluted with 0.22--0.40 M phosphate buffer (pH 6.8) from a hydroxylapatite column, was completely hydrolyzed with venom phosphodiesterase, and was separated by DEAE-...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.2.595

    authors： Miwa M,Saikawa N,Yamaizumi Z,Nishimura S,Sugimura T

    更新日期：1979-02-01 00:00:00

  • Modulation of rhythmic brain activity by diazepam: GABA(A) receptor subtype and state specificity.

    abstract：:The inhibitory neurotransmitter gamma-aminobutyric acid (GABA) is involved in the generation of various brain rhythmic activities that can be modulated by benzodiazepines. Here, we assessed the contribution of alpha(2)GABA type A (GABA(A)) receptors to the effects of benzodiazepines on sleep and waking oscillatory pat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0306975101

    authors： Kopp C,Rudolph U,Löw K,Tobler I

    更新日期：2004-03-09 00:00:00

  • Erythropoietin induces tumor regression and antitumor immune responses in murine myeloma models.

    abstract：:Recombinant human erythropoietin (rHuEpo) has been used successfully in the treatment of cancer-related anemia. Clinical observations with several patients with multiple-myeloma treated with rHuEpo has shown, in addition to the improved quality of life, a longer survival than expected, considering the poor prognostic ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.081275298

    authors： Mittelman M,Neumann D,Peled A,Kanter P,Haran-Ghera N

    更新日期：2001-04-24 00:00:00

  • Functional expression and photoaffinity labeling of a cloned P2U purinergic receptor.

    abstract：:ATP and UTP can function as extracellular signaling molecules by activating plasma membrane receptors termed P2 purinergic receptors. In the present study a P2U receptor cDNA has been expressed in K-562 human leukemia cells, one of the few available mammalian cell lines that lacks an endogenous P2U receptor. In stably...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.22.10449

    authors： Erb L,Lustig KD,Sullivan DM,Turner JT,Weisman GA

    更新日期：1993-11-15 00:00:00

  • Higher order nuclear organization: three-dimensional distribution of small nuclear ribonucleoprotein particles.

    abstract：:The structural and functional organization of the cell nucleus has been investigated using three-dimensional reconstruction, immunoelectron microscopy, and high-resolution in situ autoradiography. Nuclear regions enriched in small nuclear ribonucleoprotein particles (snRNPs) form a reticular network within the nucleop...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.1.147

    authors： Spector DL

    更新日期：1990-01-01 00:00:00

  • Genome-wide identification of tissue-specific enhancers in the Ciona tadpole.

    abstract：:Less than 100 cis-regulatory DNAs have been characterized in the context of transgenic metazoan embryos. Here we investigate the feasibility of conducting a genome-wide search for tissue-specific enhancers in the ascidian Ciona intestinalis. A total of 138 random genomic DNA fragments with an average size of 1.7 kb we...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.052024999

    authors： Harafuji N,Keys DN,Levine M

    更新日期：2002-05-14 00:00:00

  • Generalizing Fisher's "reproductive value": linear differential and difference equations of "dilute" biological systems.

    abstract：:R. A. Fisher's 1930 "reproductive value" is defined as the contribution made by a population's initial age elements to its asymptotically dominating exponential growth mode. For the Leslie discrete-time model, it is the characteristic row vector of the Leslie matrix, and for the integral-equation model of Lotka the si...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.11.5189

    authors： Samuelson PA

    更新日期：1977-11-01 00:00:00

  • Transcripts from downstream alternative transcription start sites evade uORF-mediated inhibition of gene expression in Arabidopsis.

    abstract：:Plants adapt to alterations in light conditions by controlling their gene expression profiles. Expression of light-inducible genes is transcriptionally induced by transcription factors such as HY5. However, few detailed analyses have been carried out on the control of transcription start sites (TSSs). Of the various w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1804971115

    authors： Kurihara Y,Makita Y,Kawashima M,Fujita T,Iwasaki S,Matsui M

    更新日期：2018-07-24 00:00:00

  • Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and shoot meristem of Arabidopsis.

    abstract：:Dominant mutations in the maize homeobox gene knotted1 (kn1) act nonautonomously during maize leaf development, indicating that Kn1 is involved in the generation or transmission of a developmental signal that passes from the inner layers of the leaf to epidermal cells. We previously found that this nonautonomous activ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.052484099

    authors： Kim JY,Yuan Z,Cilia M,Khalfan-Jagani Z,Jackson D

    更新日期：2002-03-19 00:00:00

  • Fundamental differences between Arctic and Antarctic ozone depletion.

    abstract：:Antarctic ozone depletion is associated with enhanced chlorine from anthropogenic chlorofluorocarbons and heterogeneous chemistry under cold conditions. The deep Antarctic "hole" contrasts with the generally weaker depletions observed in the warmer Arctic. An unusually cold Arctic stratospheric season occurred in 2011...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1319307111

    authors： Solomon S,Haskins J,Ivy DJ,Min F

    更新日期：2014-04-29 00:00:00

  • DNA probes that store mechanical information reveal transient piconewton forces applied by T cells.

    abstract：:The advent of molecular tension probes for real-time mapping of piconewton forces in living systems has had a major impact on mechanobiology. For example, DNA-based tension probes have revealed roles for mechanics in platelet, B cell, T cell, and fibroblast function. Nonetheless, imaging short-lived forces transmitted...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1904034116

    authors： Ma R,Kellner AV,Ma VP,Su H,Deal BR,Brockman JM,Salaita K

    更新日期：2019-08-20 00:00:00

  • Inhibition of acute in vivo human immunodeficiency virus infection by human interleukin 10 treatment of SCID mice implanted with human fetal thymus and liver.

    abstract：:To improve the usefulness of in vivo mode for the investigation of the pathophysiology of human immunodeficiency virus (HIV) infection, we modified the construction of SCID mice implanted with human fetal thymus and liver (thy/liv-SCID-hu mice) so that the peripheral blood of the mice contained significant numbers of ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.7.3126

    authors： Kollmann TR,Pettoello-Mantovani M,Katopodis NF,Hachamovitch M,Rubinstein A,Kim A,Goldstein H

    更新日期：1996-04-02 00:00:00

  • Signal recognition particle components in the nucleolus.

    abstract：:The signal recognition particle (SRP) is a ribonucleoprotein composed of an Alu domain and an S domain. The S domain contains unique sequence SRP RNA and four SRP proteins: SRP19, SRP54, SRP68, and SRP72. SRP interacts with ribosomes to bring translating membrane and secreted proteins to the endoplasmic reticulum (ER)...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.1.55

    authors： Politz JC,Yarovoi S,Kilroy SM,Gowda K,Zwieb C,Pederson T

    更新日期：2000-01-04 00:00:00

  • Mycobacterial Esx-3 is required for mycobactin-mediated iron acquisition.

    abstract：:The Esx secretion pathway is conserved across Gram-positive bacteria. Esx-1, the best-characterized system, is required for virulence of Mycobacterium tuberculosis, although its precise function during infection remains unclear. Esx-3, a paralogous system present in all mycobacterial species, is required for growth in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0900589106

    authors： Siegrist MS,Unnikrishnan M,McConnell MJ,Borowsky M,Cheng TY,Siddiqi N,Fortune SM,Moody DB,Rubin EJ

    更新日期：2009-11-03 00:00:00

  • Infinitesimal generators and quasi-units in potential theory.

    abstract：:The underlying structure is taken as a strongly superharmonic cone [unk], defined as a partially ordered abelian semigroup with identity 0 which admits a multiplication by nonnegative scalars and satisfies two fundamental axioms of a potentialtheoretic character. In terms of a fixed nonzero element e there is introduc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.7.2498

    authors： Arsove M,Leutwiler H

    更新日期：1975-07-01 00:00:00

  • Growing optic nerve fibers follow neighbors during embryogenesis.

    abstract：:The embryonic development of the optic nerve of the zebrafish, Brachydanio rerio, was studied by three-dimensional computer reconstruction from serial section electron micrographs. Growing fibers from retinal ganglion cells had growth cones in contact with more mature fibers from adjacent cell bodies. In the observed ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.7.4374

    authors： Bodick N,Levinthal C

    更新日期：1980-07-01 00:00:00

  • Patterning cells and their environments using multiple laminar fluid flows in capillary networks.

    abstract：:This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cell...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.10.5545

    authors： Takayama S,McDonald JC,Ostuni E,Liang MN,Kenis PJ,Ismagilov RF,Whitesides GM

    更新日期：1999-05-11 00:00:00

  • Glucocorticoid receptor-dependent disruption of a specific nucleosome on the mouse mammary tumor virus promoter is prevented by sodium butyrate.

    abstract：:Our laboratory has previously developed cell lines derived from mouse NIH 3T3 fibroblasts and C127 mammary tumor cells that stably express mouse mammary tumor virus (MMTV) long terminal repeat fusion genes in bovine papillomavirus-based episomes. Glucocorticoid hormone strongly activates transcription from episomes an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.10.3977

    authors： Bresnick EH,John S,Berard DS,LeFebvre P,Hager GL

    更新日期：1990-05-01 00:00:00

  • Tyrosine kinase inhibitors reverse type 1 diabetes in nonobese diabetic mice.

    abstract：:The recent development of small-molecule tyrosine kinase (TK) inhibitors offers increasing opportunities for the treatment of autoimmune diseases. In this study, we investigated the potential of this new class of drugs to treat and cure type 1 diabetes (T1D) in the NOD mouse. Treatment of prediabetic and new onset dia...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0810246105

    authors： Louvet C,Szot GL,Lang J,Lee MR,Martinier N,Bollag G,Zhu S,Weiss A,Bluestone JA

    更新日期：2008-12-02 00:00:00