A general assay for antibody catalysis using acridone as a fluorescent tag.

abstract：:Cubic cage compounds composed of Co-CN-Ru linkages have been prepared which illustrate the following features: (i) new motifs for alkali metal ion complexation (i.e., cationic receptors for cations), (ii) a new family of triaza-metalloligands, and (iii) a double box-like cage. The cages are synthesized by the condensa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Contakes SM,Kuhlman ML,Ramesh M,Wilson SR,Rauchfuss TB

更新日期：2002-04-16 00:00:00

abstract：:A screening procedure based on solvent extraction with minimal clean up followed by examination with negative chemical ionization mass spectrometry has been developed for organochlorine residues and related compounds with masses greater than 130 daltons. The procedure has been made quantitative for analysis of pentach...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dougherty RC,Piotrowska K

更新日期：1976-06-01 00:00:00

abstract：:Bilateria are currently subdivided into three superclades: Deuterostomia, Ecdysozoa, and Lophotrochozoa. Within this new taxonomic frame, acoelomate Platyhelminthes, for a long time held to be basal bilaterians, are now considered spiralian lophotrochozoans. However, recent 18S rDNA [small subunit (SSU)] analyses have...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruiz-Trillo I,Paps J,Loukota M,Ribera C,Jondelius U,Baguna J,Riutort M

更新日期：2002-08-20 00:00:00

abstract：:The Arabidopsis thaliana AtHKT1 protein, a Na(+)/K(+) transporter, is capable of mediating inward Na(+) currents in Xenopus laevis oocytes and K(+) uptake in Escherichia coli. HKT1 proteins are members of a superfamily of K(+) transporters. These proteins have been proposed to contain eight transmembrane segments and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kato Y,Sakaguchi M,Mori Y,Saito K,Nakamura T,Bakker EP,Sato Y,Goshima S,Uozumi N

更新日期：2001-05-22 00:00:00

abstract：:In vitro translation of Rous sarcoma virus (RSV) virion RNA in the messenger-dependent reticulocyte lysate system yielded polypeptides that were not synthesized by translation of RNA from a transformation-defective deletion mutant of RSV. These RSV-specific products migrated on sodium dodecyl sulfate/polyacrylamide ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beemon K,Hunter T

更新日期：1977-08-01 00:00:00

abstract：:Viruses have unique lifestyles. To describe the pathogenesis and significance of viral infection in terms of host responses, resultant injury, and therapy, we focused on two RNA viruses: lymphocytic choriomeningitis (LCMV) and influenza (Flu). Many of the currently established concepts and consequences about viruses a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oldstone MB

更新日期：2013-03-12 00:00:00

abstract：:Neurons in macaque primary visual cortex are spatially arranged by their global topographic position and in at least three overlapping local modular systems: ocular dominance columns, orientation pinwheels, and cytochrome oxidase (CO) blobs. Individual neurons in the blobs are not tuned to orientation, and populations...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polimeni JR,Granquist-Fraser D,Wood RJ,Schwartz EL

更新日期：2005-03-15 00:00:00

abstract：:GSTs catalyze the conjugation of glutathione with a wide variety of hydrophobic compounds, generally resulting in nontoxic products that can be readily eliminated. In contrast to many other organisms, the malarial parasite Plasmodium falciparum possesses only one GST isoenzyme (PfGST). This GST is highly abundant in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fritz-Wolf K,Becker A,Rahlfs S,Harwaldt P,Schirmer RH,Kabsch W,Becker K

更新日期：2003-11-25 00:00:00

abstract：:Cerebellar long-term depression (LTD) is a persistent attenuation of synaptic transmission at the parallel fiber-Purkinje cell synapse mediated by the removal of GluR2 subunit-containing alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. The removal of AMPA receptors requires protein kinase C p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steinberg JP,Huganir RL,Linden DJ

更新日期：2004-12-28 00:00:00

abstract：:Several mouse models have already proved valuable for investigating hypertrophic responses to cardiac stress. Here, we characterize one caused by a well defined single copy transgene, RenTgMK, that genetically clamps plasma renin and thence angiotensin II at high levels. All of the transgenic males develop concentric ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caron KM,James LR,Kim HS,Knowles J,Uhlir R,Mao L,Hagaman JR,Cascio W,Rockman H,Smithies O

更新日期：2004-03-02 00:00:00

abstract：:The seventh cholera pandemic caused by Vibrio cholerae O1 El Tor (ET) has been superseded in Asia and Africa by altered ET possessing the cholera toxin (CTX) gene of classical (CL) biotype. The CL biotype of V. cholerae was isolated, along with prototypic and altered ET, during the 1991 cholera epidemic in Mexico and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alam M,Rashed SM,Mannan SB,Islam T,Lizarraga-Partida ML,Delgado G,Morales-Espinosa R,Mendez JL,Navarro A,Watanabe H,Ohnishi M,Hasan NA,Huq A,Sack RB,Colwell RR,Cravioto A

更新日期：2014-07-08 00:00:00

abstract：:Across the Upper Missouri River Basin, the recent drought of 2000 to 2010, known as the "turn-of-the-century drought," was likely more severe than any in the instrumental record including the Dust Bowl drought. However, until now, adequate proxy records needed to better understand this event with regard to long-term v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martin JT,Pederson GT,Woodhouse CA,Cook ER,McCabe GJ,Anchukaitis KJ,Wise EK,Erger PJ,Dolan L,McGuire M,Gangopadhyay S,Chase KJ,Littell JS,Gray ST,St George S,Friedman JM,Sauchyn DJ,St-Jacques JM,King J

更新日期：2020-05-26 00:00:00

abstract：:The high resolution 1H and 31P NMR spectra of the (dG-dC)8 duplex have been recorded in low- and high-salt solutions in order to evaluate the structural aspects of the salt-induced transition of oligo(dG-dC) in solution [Pohl, F. M. & Jovin, T. M. (1972) J. Mol. Biol. 67, 375-396]. The NMR data require that the (dG-d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel DJ,Canuel LL,Pohl FM

更新日期：1979-06-01 00:00:00

abstract：:Ubiquitylation of histone H2B at lysine 120 (H2B-Ub) plays a critical role in transcriptional elongation, chromatin conformation, as well as the regulation of specific histone H3 methylations. Herein, we report a strategy for the site-specific chemical attachment of ubiquitin to preassembled nucleosomes. This allowed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holt MT,David Y,Pollock S,Tang Z,Jeon J,Kim J,Roeder RG,Muir TW

更新日期：2015-08-18 00:00:00

abstract：:Throughout most of pregnancy, uterine quiescence is maintained by increased progesterone receptor (PR) transcriptional activity, whereas spontaneous labor is initiated/facilitated by a concerted series of biochemical events that activate inflammatory pathways and have a negative impact on PR function. In this study, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renthal NE,Chen CC,Williams KC,Gerard RD,Prange-Kiel J,Mendelson CR

更新日期：2010-11-30 00:00:00

abstract：:In the plant Nicotiana plumbaginifolia, manganese superoxide dismutase (MnSOD) is synthesized in the cytoplasm as a preprotein and is subsequently translocated to the mitochondrial matrix with corresponding cleavage of an NH2-terminal leader sequence. To determine whether the plant enzyme could replace the endogenous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowler C,Alliotte T,Van den Bulcke M,Bauw G,Vandekerckhove J,Van Montagu M,Inzé D

更新日期：1989-05-01 00:00:00

abstract：:Multiple independent mutations in neuroligin genes were identified in patients with familial autism, including the R451C substitution in neuroligin-3 (NL3). Previous studies showed that NL3(R451C) knock-in mice exhibited modestly impaired social behaviors, enhanced water maze learning abilities, and increased synaptic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Etherton M,Földy C,Sharma M,Tabuchi K,Liu X,Shamloo M,Malenka RC,Südhof TC

更新日期：2011-08-16 00:00:00

abstract：:Plasmids containing the replication origin of the Escherichia coli chromosome (oriC plasmids) are unstable in certain recA strains of E. coli. However, they can be maintained more stably in other recA strains. This stable maintenance has allowed us to study the incompatibility properties of oriC plasmids. We have foun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamaguchi K,Yamaguchi M,Tomizawa J

更新日期：1982-09-01 00:00:00

abstract：:Ion channel proteins control ionic flux across biological membranes through conformational changes in their transmembrane pores. An exponentially increasing number of channel structures captured in different conformational states are now being determined; however, these newly resolved structures are commonly classifie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rao S,Klesse G,Stansfeld PJ,Tucker SJ,Sansom MSP

更新日期：2019-07-09 00:00:00

abstract：:Proteins can bind target molecules through either induced fit or conformational selection pathways. In the conformational selection model, a protein samples a scarcely populated high-energy state that resembles a target-bound conformation. In enzymatic catalysis, such high-energy states have been identified as crucial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovermann M,Grundström C,Sauer-Eriksson AE,Sauer UH,Wolf-Watz M

更新日期：2017-06-13 00:00:00

abstract：:During early postnatal development, sensory regions of the brain undergo periods of heightened plasticity which sculpt neural networks and lay the foundation for adult sensory perception. Such critical periods were also postulated for learning and memory but remain elusive and poorly understood. Here, we present evide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao X,Castro-Gomez S,Grendel J,Graf S,Süsens U,Binkle L,Mensching D,Isbrandt D,Kuhl D,Ohana O

更新日期：2018-12-04 00:00:00

abstract：:Prader-Willi syndrome (PWS) and Angelman syndrome (AS) result from the loss of function of imprinted genes in human chromosome 15q11-q13. The central part of mouse chromosome 7 is homologous to human 15q11-q13, with conservation of both gene order and imprinted features. We report here the characterization of a transg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gabriel JM,Merchant M,Ohta T,Ji Y,Caldwell RG,Ramsey MJ,Tucker JD,Longnecker R,Nicholls RD

更新日期：1999-08-03 00:00:00

abstract：:Type C retroviruses are endogenous in most vertebrate species. These viruses generally are of low pathogenicity in their natural hosts. Variants that contain cell-derived transforming genes have been isolated infrequently in the past upon continued passage in vivo. We report here a procedure that allows the isolation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rapp UR,Todaro GJ

更新日期：1980-01-01 00:00:00

abstract：:Mammalian ferritin from horse spleen undergoes an electrochemical or chemical reduction reaction in which each iron atom present is reduced by one electron (2300 electrons per ferritin molecule containing 2300 Fe3+ ions). Midpoint potentials of -190 mV, -310 mV, and -416 mV were determined at pH 7.0, 8.0, and 9.0. Thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watt GD,Frankel RB,Papaefthymiou GC

更新日期：1985-06-01 00:00:00

abstract：:The growth or decay of population of a single species interacting with a large number of other species (or environment) according to the Volterra-Lotka model is investigated. When the environment is initially very close to its equilibrium level, the growth of a single species follows a generalized Verhulst law, contai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zwanzig R

更新日期：1973-11-01 00:00:00

abstract：:The effect of reflex splanchnic nerve stimulation on proenkephalin A biosynthesis was investigated in the rat adrenal medulla. Tissue levels of native [Met5]enkephalin-like immunoreactivity (IR) (measured by direct RIA of tissue extracts), cryptic [Met5]enkephalin-like IR (calculated as the increase in [Met5]enkephali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanamatsu T,Unsworth CD,Diliberto EJ Jr,Viveros OH,Hong JS

更新日期：1986-12-01 00:00:00

abstract：:Sera from 11 individuals which were classified as Lp(a-) by direct gel diffusion and by absorption tests showed the presence of Lp(a) lipoprotein when the sera were concentrated 120-fold. This Lp(a) antigen was demonstrated by gel diffusion to be indistinguishable from Lp-lipoprotein isolated from known Lp(a+) sera. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harvie NR,Schultz JS

更新日期：1970-05-01 00:00:00

abstract：:A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vasmatzis G,Essand M,Brinkmann U,Lee B,Pastan I

更新日期：1998-01-06 00:00:00

abstract：:The A(2A)R is largely coexpressed with D(2)Rs and enkephalin mRNA in the striatum where it modulates dopaminergic activity. Activation of the A(2A)R antagonizes D(2)R-mediated behavioral and neurochemical effects in the basal ganglia through a mechanism that may involve direct A(2A)R-D(2)R interaction. However, whethe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen JF,Moratalla R,Impagnatiello F,Grandy DK,Cuellar B,Rubinstein M,Beilstein MA,Hackett E,Fink JS,Low MJ,Ongini E,Schwarzschild MA

更新日期：2001-02-13 00:00:00

abstract：:Although(,) vascular remodeling is a hallmark of many chronic inflammatory disorders such as rheumatoid arthritis, inflammatory bowel disease, and psoriasis, anti-vascular strategies to treat these conditions have received little attention to date. We investigated the anti-inflammatory activity of systemic blockade of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schonthaler HB,Huggenberger R,Wculek SK,Detmar M,Wagner EF

更新日期：2009-12-15 00:00:00