Mycobacterial Esx-3 is required for mycobactin-mediated iron acquisition.

Abstract:

:The Esx secretion pathway is conserved across Gram-positive bacteria. Esx-1, the best-characterized system, is required for virulence of Mycobacterium tuberculosis, although its precise function during infection remains unclear. Esx-3, a paralogous system present in all mycobacterial species, is required for growth in vitro. Here, we demonstrate that mycobacteria lacking Esx-3 are defective in acquiring iron. To compete for the limited iron available in the host and the environment, these organisms use mycobactin, high-affinity iron-binding molecules. In the absence of Esx-3, mycobacteria synthesize mycobactin but are unable to use the bound iron and are impaired severely for growth during macrophage infection. Mycobacteria thus require a specialized secretion system for acquiring iron from siderophores.

authors

Siegrist MS,Unnikrishnan M,McConnell MJ,Borowsky M,Cheng TY,Siddiqi N,Fortune SM,Moody DB,Rubin EJ

doi

10.1073/pnas.0900589106

subject

Has Abstract

pub_date

2009-11-03 00:00:00

pages

18792-7

issue

44

eissn

0027-8424

issn

1091-6490

pii

0900589106

journal_volume

106

pub_type

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