Abstract:
:Our earlier studies have shown that simultaneous inhibition of glycolysis and pentose phosphate pathway using 2-deoxy-d-glucose (2-DG, an inhibitor of glycolysis) and 6-aminonicotinamide (6-AN, an inhibitor of pentose phosphate pathway) lead to metabolic oxidative stress (MOS), resulting in radiosensitization in malignant cells. Present study was carried out to investigate the effects of 2-DG and 6-AN on intricately regulated endogenous antioxidant defense against MOS during radiosensitization by this combination. Two human tumor cell lines {Head and Neck Squamous carcinoma (KB) and Glioma (BMG-1)} and one non-malignantly transformed cell line (human embryonic kidney, HEK) were used in this study. The presence of 2-DG and 6-AN (added just before irradiation) for 4h, significantly decreased the clonogenicity and metabolic viability of KB and BMG-1 cell lines, while no significant change was seen in HEK cells. Accumulation of ROS was observed only in malignant cell lines, which displayed a compromised redox status evident from enhanced NADP(+)/NADPH and GSSG/GSH ratios and a concomitant decrease in glutathione reductase level and activity at 24h following treatment. The levels and activities of Cu, Zn-SOD and Mn-SOD increased with MOS and were accompanied by a decreased GPx and unaltered catalase activity and level. These results suggest that non-coordinated expression of antioxidant defense, besides compromised redox status, led to selective radiosensitization in the malignant cells.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Sharma PK,Bhardwaj R,Dwarakanath BS,Varshney Rdoi
10.1016/j.canlet.2010.02.021subject
Has Abstractpub_date
2010-09-28 00:00:00pages
154-66issue
2eissn
0304-3835issn
1872-7980pii
S0304-3835(10)00122-9journal_volume
295pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::The constitutional expression of heat shock proteins (HSP) 27, 70 and 90 in human breast, colon and ovarian cancer cells transplanted into severe combined immunodeficient (SCID) mice was evaluated. In addition their induced expression under chemotherapeutic stress was analyzed. The oestrogen receptor positive breast c...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00601-7
更新日期:2000-12-08 00:00:00
abstract::The influence of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone, a mutagen in chlorinated water, on cell proliferation and lipid peroxidation in the glandular stomach mucosa was investigated in male 4-week-old Wistar rats. Animals were given 50 p.p.m., 25 p.p.m., 12.5 p.p.m., 6.25 p.p.m. or 0 p.p.m. of MX soluti...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(94)90268-2
更新日期:1994-10-14 00:00:00
abstract::Elevated cyclooxygenase-2 (COX-2) expression has been observed in various types of cancer. Induction of COX-2 expression has been reported to increase invasiveness and angiogenesis of tumours. While COX-2 overexpression has been repeatedly proven to promote tumor growth, little is known about what initiates its induct...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2005.03.023
更新日期:2006-02-28 00:00:00
abstract::Sulofenur treatment (12.5 microM-1 mM) of colon adenocarcinoma cell lines resulted in dose- and time-dependent cell killing. LYc5 cells were viable longer than GC3/c1 cells. Each concentration resulted in elevation of cytosolic calcium [Ca2+]i) for both cell lines. At lower doses, elevation was delayed for LYc5 cells....
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(94)03607-k
更新日期:1995-01-06 00:00:00
abstract::Most brain tumors are highly resistant to chemotherapy because many chemotherapeutic drugs poorly cross the blood-brain barrier, the blood-cerebrospinal-fluid barrier, and the plasma membrane of the tumor cells. This restricted drug delivery is largely due to the presence of integral plasma membrane proteins belonging...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2006.12.023
更新日期:2007-08-28 00:00:00
abstract::In esophageal squamous cell carcinoma (SCC), we used immunohistochemical analysis to further elucidate the correlation of p53 protein expression with clinicopathological factors, as well as with risk factors, such as tobacco smoking, alcohol consumption and a family history of cancer, using odds ratios (ORs). The expr...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(01)00461-x
更新日期:2001-06-10 00:00:00
abstract::TGF-β signaling plays an important role in breast cancer progression and metastasis. Epithelial-mesenchymal transition (EMT) is an important step in the progression of solid tumors to metastatic disease. We previously reported that IN-1130, a novel transforming growth factor-β type I receptor kinase (ALK5) inhibitor, ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2014.05.006
更新日期:2014-08-28 00:00:00
abstract::Trypsin inhibitors have been shown to promote pancreatic growth as well as the development of pancreatic tumours in rats. The present study was carried out to examine the effects of the synthetic trypsin inhibitor camostate on the growth of the pancreas and on the development of pancreatic preneoplastic and neoplastic...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(91)90115-x
更新日期:1991-12-01 00:00:00
abstract::We have examined the expression of nitrotyrosine, a marker of peroxynitrite formation, in 55 esophageal cancers by immunohistochemistry. Nitrotyrosine was detected in 21 of 55 (38.2%) esophageal cancers. Comparison of nitrotyrosine expression and the pathological findings showed that there was a significant associatio...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00358-x
更新日期:2000-05-29 00:00:00
abstract::Analysis of the loss of heterozygosity (LOH) detected by polymerase chain reaction techniques using 18 polymorphic markers localized to chromosomes 3p, 5, 17, and 18q in 40 Hong Kong Chinese esophageal squamous cell carcinoma (ESC) patients showed that multiple alterations on several chromosomes are involved in ESC de...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(01)00577-8
更新日期:2001-09-20 00:00:00
abstract::Staurosporine and its derivative 7-hydroxystaurosporine are protein kinase inhibitors that are being considered for treatments of cancers. Several recent studies have shown that cells with defective pRB protein are resistant to the G(1) cell cycle-inhibiting effects of staurosporine compounds. In this study, we examin...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(02)00101-5
更新日期:2002-09-08 00:00:00
abstract::The antitumor effects of 2'-C-cyano-2'-deoxy-1-beta-D- arabinofuranosylcytosine (CN-DAC), a synthetic 1-beta-D-arabinofuranosyl-cytosine (ara-C) derivative, were examined and compared with that of ara-C in murine tumors and in various human tumors using three different chemosensitivity tests. CNDAC extended the life s...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(92)90024-p
更新日期:1992-05-30 00:00:00
abstract::Gene delivery vectors, including adenovirus (Ad) and adeno-associated virus (AAV), are inefficient and non-selective for cancer due to low levels of viral receptors with high levels on other tissues, including liver. We tested Ads and AAVs with the SIGYPLP-targeting peptide inserted into virus capsids for transduction...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2003.07.003
更新日期:2003-11-25 00:00:00
abstract::The environmental mutagens, 1-nitropyrene, 1,3-dinitropyrene, 1,6-dinitropyrene and 1,8-dinitropyrene were evaluated for their ability to initiate skin tumors in male and female SENCAR mice. 1-Nitropyrene (greater than 99.5% purity) did not induce papilloma formation over a dose range of 0-3.0 mg/mouse after 30 weeks ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(84)90055-7
更新日期:1984-05-01 00:00:00
abstract::This review article provides a comprehensive overview of the experimental data detailing the incidence, mechanism and significance of low dose hyper-radiosensitivity (HRS). Important discoveries gained from past and present studies are mapped and highlighted to illustrate the pathway to our current understanding of HR...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2013.12.015
更新日期:2014-07-10 00:00:00
abstract::Gastric cancer (GC) is a major threat to human health, and its prognosis is poor due to the lack of appropriate biomarkers. LncRNAs are a group of non-protein-coding RNAs that regulate gene expression at the transcriptional or posttranscriptional level. LncRNAs play essential roles in GC initiation and development in ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.11.011
更新日期:2016-02-01 00:00:00
abstract::Human epidermal growth factor receptor 2 (HER2) is an attractive target for cancer vaccine. However, autoimmune tolerance prevents vaccines based on HER2 protein from inducing long-lasting, highly effective anti-tumor immunity. In this study, we proved that the introduction of p-nitrophenylalanine in the universal T c...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2018.05.021
更新日期:2018-08-28 00:00:00
abstract::Gene-silencing with targeted siRNAs has great potential as a therapeutic approach for various diseases including cancer. However, intracellular delivery of siRNA is challenging. We used bovine milk exosomes as a novel system for siRNA delivery. First, we demonstrated that exosomes can deliver endogenous RNA payloads i...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.02.011
更新日期:2019-05-01 00:00:00
abstract::Breast fluid which bathes the mammary ductal epithelium may contain tumorogenic components. In view of the number of tobacco components inhaled during smoking, it was of interest whether nicotine and its metabolites were present in this fluid. Fluid was obtained from the nipple from menstruating women who were smokers...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(79)80042-7
更新日期:1979-04-01 00:00:00
abstract::Despite being the subject of extensive research and clinical trials, neuroblastoma remains a major therapeutic challenge in pediatric oncology. The p53 protein is a central safeguard that protects cells against genome instability and malignant transformation. Mutated TP53 (the gene encoding p53) is implicated in many ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.09.023
更新日期:2021-01-01 00:00:00
abstract::We have developed a simple system for rapid detection and measurement of glutathione S-transferase placental form (GSTP1) that detoxify polycyclic aromatic hydrocarbons using the cultured rat normal liver epithelial cell line, (RL34) cells. Survey of fruit extracts for GST inducing ability identified both papaya and a...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00487-0
更新日期:2000-09-01 00:00:00
abstract::These experiments were designed to test the role of the polyamine pathway in breast cancer progression utilizing an experimental system based on the development of ovary-independent rat mammary tumors and their sequential transplantation into syngeneic hosts. Three key enzymes involved in the PA biosynthetic/catabolic...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(95)03763-m
更新日期:1995-05-25 00:00:00
abstract::The present study evaluates the postnatal efficacy of Momordica charantia peel, pulp, seed and whole fruit extract via assessing the modulation in the biotransformation system enzymes of suckling neonates and lactating mice. The peel, seed or whole fruit extract of Momordica (100 microl/animal/day) independently induc...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(97)00381-9
更新日期:1998-01-09 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest of all solid tumours and more effective therapy is urgently needed. The stroma is thought to play a critical role in tumour development and metastasis, and high stromal expression of the matricellular protein SPARC has been robustly associated with poor p...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2020.02.031
更新日期:2020-05-01 00:00:00
abstract::Chromosome 22q shows a high frequency of loss of heterozygosity (LOH) in ovarian cancers suggesting the existence of one or more important tumor suppressor genes (TSGs). The tissue inhibitor of metalloproteinase-3 (TIMP-3) is a plausible TSG candidate since it is often encompassed within these regions of LOH. TIMP-3 h...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2006.03.024
更新日期:2007-03-08 00:00:00
abstract::Dihydroambazone (DHA) is a water-soluble derivative of the experimental anticancer drug ambazone. In vitro, a combination of DHA and human recombinant tumor necrosis factor alpha (TNF) exerted a strong synergism of cytotoxicity against both mouse melanoma B16K cells and the TNF-sensitive mouse fibroblast line L-M (S)....
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(90)90032-s
更新日期:1990-11-05 00:00:00
abstract::Glioblastoma (GBM) is a deadly and incurable brain tumor. Although microRNAs (miRNAs) play critical roles in regulating the cancer cell phenotype, the underlying mechanisms of how they regulate tumorigenesis are incompletely understood. We found that miR-1 is expressed at relatively low levels in brain cancer patients...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.08.021
更新日期:2019-11-28 00:00:00
abstract::Expression of the MUC2 gene is controlled by the methylation of CpG sites in the promoter region, but the detailed methylation status of this region has yet to be reported. We have mapped the complete methylation status of the MUC2 promoter from position -1989 to position +288 upstream, a region that contains 59 CpG s...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2004.11.058
更新日期:2005-09-28 00:00:00
abstract::The pineal has been recently implicated in mammary tumorigenesis. Effect of physiological pinealectomy brought about by subjecting female Holtzman rats to permanent lighting (24 h/day) from birth was studied on the incidence of 9,10-dimethyl-1,2-benzanthracene (DMBA) induced mammary tumors. The incidence of adenocarci...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(82)90012-x
更新日期:1982-09-01 00:00:00
abstract::Prolonged exposure to elevated levels of estrogen is a risk factor for breast cancer. Though increased cell growth and loss of DNA repair capacity is one of the proposed mechanisms for estrogen-induced cancers, the mechanism through which estrogen induces cell growth and decreases DNA repair capacity is not clear. DNA...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2011.10.022
更新日期:2012-03-01 00:00:00