Staurosporine-induced G(1) arrest in cancer cells depends on an intact pRB but is independent of p16 status.


:Staurosporine and its derivative 7-hydroxystaurosporine are protein kinase inhibitors that are being considered for treatments of cancers. Several recent studies have shown that cells with defective pRB protein are resistant to the G(1) cell cycle-inhibiting effects of staurosporine compounds. In this study, we examined the effect of staurosporine on two breast cancer-derived and three lung cancer-derived cell lines characterized by deficiencies in the p16 tumor suppressor. All of these p16-deficient cell lines are highly sensitive to staurosporine-induced inhibition of pRB phosphorylation and induction of arrest in G(1). This response is similar to that seen in cultured normal human bronchial epithelial cells and normal mammary epithelial cells, but strikingly different than the staurosporine resistance seen in cancer cells with defective pRB. Interestingly, inhibition of pRB phosphorylation could be seen within 4 h of treatment, suggesting that this inhibition is a consequence of direct effects of staurosporine on protein kinase(s) rather than a result of induction of other cyclin-dependent kinase inhibitors. Our findings suggest that different types of cancer cells have vastly different responses to the staurosporine class of agents, and that evaluation of pRB and p16 will help predict the response of the cancer cells to these agents.


Cancer Lett


Cancer letters


Zhou W,Lin Y,Wersto R,Chrest J,Gabrielson E





Has Abstract


2002-09-08 00:00:00














  • Intussusceptive microvascular growth in tumors.

    abstract::Intussusception is an alternative to the sprouting mode of angiogenesis. The advantage of this mechanism of vascular growth is that blood vessels are generated more rapidly and the capillaries thereby formed are less leaky. This review article summarizes our current knowledge concerning the role played by intussuscept...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Ribatti D,Djonov V

    更新日期:2012-03-28 00:00:00

  • TGF-β1 acts through miR-155 to down-regulate TP53INP1 in promoting epithelial-mesenchymal transition and cancer stem cell phenotypes.

    abstract::It has been shown that acquisition of epithelial-mesenchymal transition (EMT) and induction of cancer stem cell (CSC)-like properties contribute to metastasis of cancers in many studies; however, the molecular mechanisms underlying EMT and CSC phenotypes in liver cancer cells remain to be elucidated. MiR-155 is an imp...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Liu F,Kong X,Lv L,Gao J

    更新日期:2015-04-10 00:00:00

  • Preferential enlargement of leukemia cells using cytoskeletal-directed agents and cell cycle growth control parameters to induce sensitivity to low frequency ultrasound.

    abstract::Sonodynamic therapy (SDT) is a form of ultrasound therapy that has been shown to preferentially damage malignant cells based on the relatively enlarged size and altered cytology of neoplastic cells in comparison to normal cells. This study sought to determine whether cytoskeletal-directed agents that either disrupt (c...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Trendowski M,Wong V,Zoino JN,Christen TD,Gadeberg L,Sansky M,Fondy TP

    更新日期:2015-05-01 00:00:00

  • Potential of endogenous formation of volatile nitrosamines from Nigerian vegetables and spices.

    abstract::Nitrosatability of dried Nigerian vegetables and spices was investigated under simulated gastric conditions. N-Nitroso-dimethylamine (NDMA) was the only volatile nitrosamine found above the preformed level except in ugwu where N-nitroso-piperidine (2.3 ppb) was also detected. The lowest NDMA value of 0.4 ppb was found...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Atawodi SE,Maduagwu EN,Preussmann R,Spiegelhalder B

    更新日期:1991-05-24 00:00:00

  • A redox ruthenium compound directly targets PHD2 and inhibits the HIF1 pathway to reduce tumor angiogenesis independently of p53.

    abstract::Targeting specific tumor metabolic needs represents an actively investigated therapeutic strategy to bypass tumor resistance mechanisms. In this study, we describe an original approach to impact the cancer metabolism by exploiting the redox properties of a ruthenium organometallic compound. This organometallic complex...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Vidimar V,Licona C,Cerón-Camacho R,Guerin E,Coliat P,Venkatasamy A,Ali M,Guenot D,Le Lagadec R,Jung AC,Freund JN,Pfeffer M,Mellitzer G,Sava G,Gaiddon C

    更新日期:2019-01-01 00:00:00

  • Long noncoding RNAs as potential biomarkers in gastric cancer: Opportunities and challenges.

    abstract::Gastric cancer (GC) is a major threat to human health, and its prognosis is poor due to the lack of appropriate biomarkers. LncRNAs are a group of non-protein-coding RNAs that regulate gene expression at the transcriptional or posttranscriptional level. LncRNAs play essential roles in GC initiation and development in ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yang Z,Guo X,Li G,Shi Y,Li L

    更新日期:2016-02-01 00:00:00

  • Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell line.

    abstract::To obtain an optimal condition for ultrasound (US)-induced apoptosis that could be useful for cancer therapy, we applied low intensity pulsed US to sonicate U937 cells in vitro. Cells were then incubated at different time intervals before measuring apoptosis. The apoptosis was assessed by DNA fragmentation and phospha...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Feril LB Jr,Kondo T,Cui ZG,Tabuchi Y,Zhao QL,Ando H,Misaki T,Yoshikawa H,Umemura S

    更新日期:2005-04-28 00:00:00

  • Young women, cervical intraepithelial neoplasia and human papillomavirus: risk factors for persistence and recurrence.

    abstract::Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections (STIs) in young women. They can occur in one or multiple areas of the female genitalia. Usually, the vulva is the initial site of implantation for HPV. The purpose of our study is to evaluate the epidemiological aspects, inc...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Frega A,Stentella P,De Ioris A,Piazze JJ,Fambrini M,Marchionni M,Cosmi EV

    更新日期:2003-07-10 00:00:00

  • Magnetic polyethyleneimine (PEI) microcapsules as retrievable traps for carcinogen electrophiles formed in the gastrointestinal tract.

    abstract::Semi-permeable magnetic microcapsules containing polyethyleneimine (PEI) have been developed as retrievable carcinogen traps. In vitro, the soluble core PEI and membrane both bound reactive substances of limited aqueous stability, such as from [14C]N-methyl-N-nitrosourea ([14C]NMU), and aqueous stable dyes of molecula...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Povey AC,Bartsch H,O'Neill IK

    更新日期:1987-07-01 00:00:00

  • Suppression of heat-induced hsp72 accumulation by cisplatin in human glioblastoma cells.

    abstract::The accumulation of the inducible hsp72 (72-kDa heat shock protein) after hyperthermia and/or cisplatin treatment in human glioblastoma cell line (A-172) was studied by Western blot analysis. The level of hsp72 increased to eight-fold 10 h after hyperthermia alone (44 degrees C for 20 min, D50) and to three-fold 10 h ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Matsumoto H,Hayashi S,Shioura H,Ohtsubo T,Ohnishi T,Kano E

    更新日期:1996-12-20 00:00:00

  • Tumor exposed-lymphatic endothelial cells promote primary tumor growth via IL6.

    abstract::Solid tumors are composed of tumor cells and stromal cells including lymphatic endothelial cells (LEC), which are mainly viewed as cells forming lymphatic vessels involved in the transport of metastatic and immune cells. We here reveal a new mechanism by which tumor exposed-LEC (teLEC) exert mitogenic effects on tumor...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Van de Velde M,Ebroin M,Durré T,Joiret M,Gillot L,Blacher S,Geris L,Kridelka F,Noel A

    更新日期:2021-01-28 00:00:00

  • Migration and metalloproteinases determine the invasive potential of mouse melanoma cells, but not melanin and telomerase.

    abstract::The biological characteristic cell locomotion and invasion, melanin content, metalloproteinases and telomerase activity were studied in a parental mouse melanoma cell line B16 and two descendents B16BL6 and B16F10. The invasive potential of melanoma cells was assayed in a transwell cell culture chamber. Melanin conten...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Zhao W,Liu H,Xu S,Entschladen F,Niggemann B,Zänker KS,Han R

    更新日期:2001-01-01 00:00:00

  • Reduced UV-induced mutations in human osteosarcoma cells stably expressing transfected wild-type p53 cDNA.

    abstract::We constructed the plasmid which can express human wild-type p53 cDNA and introduced it into the human osteosarcoma cell line SAOS-2 that lacks the chromosomal p53 gene. A cell clone stably expressing p53 protein was isolated and UV sensitivity and UV-induced mutation frequencies of the clone were examined. The UV sen...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yagi T,Mohri-Nakanishi K,Matsuda T,Yamagishi N,Miyakoshi J,Takebe H

    更新日期:1998-01-16 00:00:00

  • Neoxanthin and fucoxanthin induce apoptosis in PC-3 human prostate cancer cells.

    abstract::Neoxanthin and fucoxanthin, which have the characteristic structure of 5,6-monoepoxide and an allenic bond, were previously found to reduce the viability of human prostate cancer cells most intensively among 15 dietary carotenoids tested. In the present study, the induction of apoptosis in PC-3 cells by these two caro...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Kotake-Nara E,Asai A,Nagao A

    更新日期:2005-03-18 00:00:00

  • Elevated levels of MDM-2 and p53 expression are associated with high grade non-Hodgkin's lymphomas.

    abstract::The role of p53 in the evolution of non-Hodgkin's lymphomas (NHL) is unclear. Mutations of the p53 gene appear to be relatively uncommon but stabilized p53 protein, as detected by immunohistochemistry, has indicated a more frequent involvement of p53. As dysfunction of p53 protein has also been suggested to occur afte...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Finnegan MC,Goepel JR,Royds J,Hancock BW,Goyns MH

    更新日期:1994-11-11 00:00:00

  • Genistein potentiates the effect of arsenic trioxide against human hepatocellular carcinoma: role of Akt and nuclear factor-κB.

    abstract::Hepatocellular carcinoma (HCC) is a highly lethal malignancy mostly because of de novo and acquired resistance to conventional chemotherapy. Constitutive activation of Akt and nuclear factor-κB (NF-κB) represent major cellular abnormalities associated with both the pathogenesis and therapeutic resistance of HCC. The a...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Ma Y,Wang J,Liu L,Zhu H,Chen X,Pan S,Sun X,Jiang H

    更新日期:2011-02-01 00:00:00

  • Application of the polymerase chain reaction (PCR) to quantify micro-metastasis in an experimental animal.

    abstract::In vitro cultured r/mHM-SFME-1 cells were injected into the hind foot pads of Balb/c mice. Metastasis was detected in the lungs of tumor-bearing mice by means of both PCR and histological methods. Primers for the PCR were set to amplify a 128 bp exon-1 sequence of the human c-Ha-ras1 gene which had been introduced int...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Matano S,Ryoyama K,Nakamura S,Okada G,Nomura T

    更新日期:1995-05-04 00:00:00

  • Inhibition of Ca(2+)-activated Cl(-) channel ANO1/TMEM16A expression suppresses tumor growth and invasiveness in human prostate carcinoma.

    abstract::The etiology of prostatic adenocarcinoma remains unclear. Prostate cancer cells of varying metastatic potential and apoptotic resistance show altered expression of plasma membrane ion channels and unbalanced Ca2+ homeostasis. Ca(2+)-activated Cl(-) channels (CaCCs) are robustly expressed in epithelial cells and functi...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Liu W,Lu M,Liu B,Huang Y,Wang K

    更新日期:2012-12-29 00:00:00

  • ZNF32 induces anoikis resistance through maintaining redox homeostasis and activating Src/FAK signaling in hepatocellular carcinoma.

    abstract::Tumor cells need to attain anoikis resistance to survive prior to metastasis making it a vital trait of malignancy. The molecular mechanism by which hepatocellular carcinoma (HCC) cells resist anoikis remains not fully understood. Here, we report that ZNF32 expression is markedly upregulated in HCC cells upon detachme...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Li K,Zhao G,Ao J,Gong D,Zhang J,Chen Y,Li J,Huang L,Xiang R,Hu J,Lin P,Wei Y

    更新日期:2019-02-01 00:00:00

  • The influence of asbestos dust on the oncogenic transformation of C3H10T 1/2 cells.

    abstract::The cell transforming ability of asbestos dust was investigated using C3H10T 1/2 murine fibroblasts. In a series of experiments both crocidolite and amosite caused no increase in the number of transformed foci over that seen in cultures from untreated cells. The dusts, were, however, capable of augmenting the oncogeni...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Brown RC,Poole A,Fleming GT

    更新日期:1983-03-01 00:00:00

  • Abivertinib, a novel BTK inhibitor: Anti-Leukemia effects and synergistic efficacy with homoharringtonine in acute myeloid leukemia.

    abstract::Ibrutinib, an inhibitor of Bruton tyrosine kinase (BTK), has shown promising pharmacologic effects in acute myeloid leukemia (AML). In this study, we report that abivertinib or AC0010, a novel BTK inhibitor, inhibits cell proliferation, reduces colony-forming capacity, and induces apoptosis and cell cycle arrest in AM...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Huang S,Pan J,Jin J,Li C,Li X,Huang J,Huang X,Yan X,Li F,Yu M,Hu C,Jin J,Xu Y,Ling Q,Ye W,Wang Y,Jin J

    更新日期:2019-10-01 00:00:00

  • A comparison of adrenergic receptors of rat ascites hepatoma AH130 cells with those of normal rat hepatocytes.

    abstract::The pharmacological specificity of adrenergic receptors in the plasma membrane of rat ascites hepatoma AH130 cells was compared with that in normal rat hepatocytes. The number of [125I]iodocyanopindolol-binding sites was much greater in AH130 cells than in the hepatocytes. We characterized the alpha-adrenergic recepto...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Sanae F,Miyamoto K,Koshiura R

    更新日期:1988-04-01 00:00:00

  • Anti-tumor and radiosensitization activities of the iron chelator HDp44mT are mediated by effects on intracellular redox status.

    abstract::A novel iron chelator, HDp44mT, has been reported to have potent anti-proliferative effects on cancer cells; however, the underlying mechanism of action is not well understood. In this study, we characterized the cytotoxic effect of HDp44mT in a chemo- and radio-resistant cell line (PC-3) of prostatic cancer origin. T...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Tian J,Peehl DM,Zheng W,Knox SJ

    更新日期:2010-12-08 00:00:00

  • Knockdown of long non-coding RNA XIST exerts tumor-suppressive functions in human glioblastoma stem cells by up-regulating miR-152.

    abstract::Glioblastoma (GBM) is the most common and aggressive primary brain tumor. Great interest persists in useful therapeutic targets in GBM. Aberrant expression of long non-coding RNAs (lncRNAs) has been functionally associated with many cancers. Here, we elucidated the function and the possible molecular mechanisms of lnc...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yao Y,Ma J,Xue Y,Wang P,Li Z,Liu J,Chen L,Xi Z,Teng H,Wang Z,Li Z,Liu Y

    更新日期:2015-04-01 00:00:00

  • Increased in vitro and in vivo sensitivity of BRCA2-associated pancreatic cancer to the poly(ADP-ribose) polymerase-1/2 inhibitor BMN 673.

    abstract::BRCA2-associated pancreatic ductal adenocarcinoma (PDAC) may be sensitive to agents that target homology-directed DNA repair, such as DNA crosslinking agents (DCLs) and PARP inhibitors (PARPis). Here, we assessed the sensitivities of BRCA2-deficient (Capan-1) and BRCA2-proficient (MIA PaCa-2) PDAC cell lines to a pane...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Andrei AZ,Hall A,Smith AL,Bascuñana C,Malina A,Connor A,Altinel-Omeroglu G,Huang S,Pelletier J,Huntsman D,Gallinger S,Omeroglu A,Metrakos P,Zogopoulos G

    更新日期:2015-08-01 00:00:00

  • Anti-angiogenic activity of ursodeoxycholic acid and its derivatives.

    abstract::Ursodeoxycholic acid (UDCA) and its derivatives were examined for anti-angiogenic activities by using the chick embryo chorioallantoic membrane (CAM) assay. The presence of UDCA or its derivatives inhibited angiogenesis in a dose-dependent manner; the dose required for half-maximal inhibition (ID50) was 4 microg per C...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Suh H,Jung EJ,Kim TH,Lee HY,Park YH,Kim KW

    更新日期:1997-02-26 00:00:00

  • Smoking is associated with increased telomerase activity in short-term cultures of human bronchial epithelial cells.

    abstract::Telomerase plays an important role in the maintenance of telomere ends in normal and tumor cells and ectopic expression can immortalize human bronchial epithelial (HBE) cells. We assessed telomerase activation, growth properties and methylation status in the hTERT promoter in a panel of HBE cell cultures in relation t...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yim HW,Slebos RJ,Randell SH,Umbach DM,Parsons AM,Rivera MP,Detterbeck FC,Taylor JA

    更新日期:2007-02-08 00:00:00

  • A combination of a DNA-chimera siRNA against PLK-1 and zoledronic acid suppresses the growth of malignant mesothelioma cells in vitro.

    abstract::Although novel agents effective against malignant mesothelioma (MM) have been developed, the prognosis of patients with MM is still poor. We generated a DNA-chimeric siRNA against polo-like kinase-1 (PLK-1), which was more stable in human serum than the non-chimeric siRNA. The chimeric PLK-1 siRNA inhibited MM cell pr...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Kawata E,Ashihara E,Nakagawa Y,Kiuchi T,Ogura M,Yao H,Sakai K,Tanaka R,Nagao R,Yokota A,Takeuchi M,Kimura S,Hirai H,Maekawa T

    更新日期:2010-08-28 00:00:00

  • An overview of current and anticipated methods for cancer prevention.

    abstract::Cancer prevention techniques include legislative regulation, chemoprevention, adherence to a "healthful" lifestyle, and specialized medical intervention. Attempts at cancer prevention have possibly avoided a dramatic increase in cancer incidence and mortality due to increasing dependence on the products of the chemica...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Clayson DB

    更新日期:1990-04-09 00:00:00

  • Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: preliminary findings.

    abstract::In the present study a methodological approach is taken which quantitates aberrant dysplastic crypts in the unsectioned murine colon. C57BL/6J or CF1 female mice (7-8 weeks old) were injected (i.p.) with azoxymethane (5 mg/kg body wt./week) for 4 weeks. Their colons were excised, cut open on the median axis and fixed ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Bird RP

    更新日期:1987-10-30 00:00:00