Structures and reaction pathways of the molybdenum centres of sulfite-oxidizing enzymes by pulsed EPR spectroscopy.

abstract：:CYP74C3 (cytochrome P450 subfamily 74C3), an HPL (hydroperoxide lyase) from Medicago truncatula (barrel medic), and CYP74A1, an AOS (allene oxide synthase) from Arabidopsis thaliana, are key membrane-associated P450 enzymes in plant oxylipin metabolism. Both recombinant detergent-free enzymes are monomeric proteins wi...

journal_title：Biochemical Society transactions

authors： Hughes RK,Belfield EJ,Casey R

更新日期：2006-12-01 00:00:00

abstract：:Many Zn(II)2Cys6 transcriptional regulators exhibit changes in phosphorylation that are coincident with their roles in transcriptional activation. It is, however, unclear whether these changes occur as a cause of, or as a result of, transcriptional activation. In this paper, we explore the relationship between these t...

journal_title：Biochemical Society transactions

authors： Leverentz MK,Reece RJ

更新日期：2006-11-01 00:00:00

abstract：:Photoreaction centres are Nature's solar batteries. These nanometre-scale power producers are responsible for transducing the energy of sunlight into a form that can be used by biological systems, thereby powering most of the biological activity on the planet. Although to the layman the word 'photosynthesis' is usuall...

journal_title：Biochemical Society transactions

authors： Jones MR

更新日期：2009-04-01 00:00:00

abstract：:Using the hyperthermophile Pyrococcus furiosus, we have delineated several key steps in CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) invader defence pathways. P. furiosus has seven transcriptionally active CRISPR loci that together encode a total of 200 crRNAs (CRISPR RNAs...

journal_title：Biochemical Society transactions

authors： Terns RM,Terns MP

更新日期：2013-12-01 00:00:00

abstract：:The UPR (unfolded protein response) or ER (endoplasmic reticulum) stress response was first described 20 years ago. The field of ER stress has expanded tremendously since, moving from basic biology in yeast to human neurodegenerative, inflammatory, cardiovascular and neoplastic diseases. The ER stress response has als...

journal_title：Biochemical Society transactions

authors： Cnop M,Igoillo-Esteve M,Cunha DA,Ladrière L,Eizirik DL

更新日期：2008-10-01 00:00:00

abstract：:The examples shown here illustrate the power of gene targeting to the epidermis as a means of developing animal models for the study of human skin diseases. In this short review, I have focused on contributions which stem predominantly from my own laboratory [31, 32, 34, 37, 47]. However, other laboratories have also ...

journal_title：Biochemical Society transactions

authors： Fuchs E

更新日期：1991-11-01 00:00:00

abstract：:Homologous recombination is essential for accurate chromosome segregation during meiosis in most sexual organisms. Meiotic recombination is initiated by the formation of DSBs (DNA double-strand breaks) made by the Spo11 protein. We review here recent findings pertaining to protein-protein interactions important for DS...

journal_title：Biochemical Society transactions

authors： Keeney S,Neale MJ

更新日期：2006-08-01 00:00:00

abstract：:Much work has gone into understanding the physical basis of the enormous catalytic power of enzymes over the last 50 years or so. Nevertheless, the detailed mechanism used by Nature's catalysts to speed chemical transformations remains elusive. DHFR (dihydrofolate reductase) has served as a paradigm to study the relat...

journal_title：Biochemical Society transactions

authors： Allemann RK,Evans RM,Loveridge EJ

更新日期：2009-04-01 00:00:00

abstract：:Intracellular cross-talk or synergism between receptors coupled negatively to adenylate cyclase via PTX-sensitive Gi-protein(s) and receptors coupled to phospholipase C via PTX-insensitive G-proteins is prevalent in a variety of cell types (see above) and may represent a universal mechanism for the modulation of phosp...

journal_title：Biochemical Society transactions

authors： Dickenson JM,Hill SJ

更新日期：1993-11-01 00:00:00

abstract：:The mechanical function and the collagen phenotype of the disc are complex, each a hybrid of elements of ligament and cartilage. In detail, the collagen properties are unique. Collagens I and II provide the bulk of the tissue fabric interwoven in opposing radial concentration gradients. From analysis of isolated cross...

journal_title：Biochemical Society transactions

authors： Eyre DR,Matsui Y,Wu JJ

更新日期：2002-11-01 00:00:00

abstract：:PLUNC (palate, lung and nasal epithelium clone) is a small, secreted protein that is expressed in the oropharynx and upper airways of humans, mice and rats. We have described a family of at least 14 PLUNC genes localized on chromosome 20 (in humans), 2 (in mice) or 3 (in rats). These rapidly evolving proteins are stru...

journal_title：Biochemical Society transactions

authors： Bingle CD,Craven CJ

更新日期：2003-08-01 00:00:00

abstract：:A combination of EXAFS, transmission electron microscopy and energy-dispersive X-ray was used to conduct a molecular and atomic analysis of the uranium complexes formed by Acidithiobacillus ferrooxidans. The results demonstrate that this bacterium accumulates uranium as phosphate compounds. We suggest that at toxic le...

journal_title：Biochemical Society transactions

authors： Merroun M,Hennig C,Rossberg A,Geipel G,Reich T,Selenska-Pobell S

更新日期：2002-08-01 00:00:00

abstract：:Mutations in MYO7A (myosin VIIa) cause Usher syndrome type 1B, a disorder involving profound congenital deafness and progressive blindness. In the retina, most MYO7A is localized in the apical region of the RPE (retinal pigmented epithelial) cells, and a small amount is associated with the ciliary and periciliary memb...

journal_title：Biochemical Society transactions

authors： Williams DS,Lopes VS

更新日期：2011-10-01 00:00:00

abstract：:A general strategy is described for the amplified expression, purification and characterization in Escherichia coli of multidrug efflux proteins from Staphylococcus aureus, Bacillus subtilis, Methanococcus janaschii and E. coli. They all catalyse drug/H(+) antiport of substrates such as quinolones and ethidium and exe...

journal_title：Biochemical Society transactions

authors： Henderson PJ,Hoyle CK,Ward A

更新日期：2000-01-01 00:00:00

abstract：:Glyoxalase- and methylglyoxal-related research has required the development of quantitative and reliable techniques for the measurement of methylglyoxal-derived glycation adducts of protein and DNA. There are also other glycation adducts, oxidation adducts and nitration adducts of proteins and oxidation adducts of DNA...

journal_title：Biochemical Society transactions

authors： Rabbani N,Shaheen F,Anwar A,Masania J,Thornalley PJ

更新日期：2014-04-01 00:00:00

abstract：:The Cdc (cell division cycle) 45 protein has a central role in the regulation of the initiation and elongation stages of eukaryotic chromosomal DNA replication. In addition, it is the main target for a Chk1 (checkpoint kinase 1)-dependent Cdc25/CDK2 (cyclin-dependent kinase 2)-independent DNA damage checkpoint signal ...

journal_title：Biochemical Society transactions

authors： Broderick R,Nasheuer HP

更新日期：2009-08-01 00:00:00

abstract：:Intracellular pathogens survive in eukaryotic cells by evading a variety of host defences. To avoid degradation through the endocytic pathway, intracellular bacteria must adapt their phagosomes into protective compartments that promote bacterial replication. Legionella pneumophila and Coxiella burnetii are Gram-negati...

journal_title：Biochemical Society transactions

authors： Hardiman CA,McDonough JA,Newton HJ,Roy CR

更新日期：2012-12-01 00:00:00

abstract：:Sister-chromatid cohesion, thought to be primarily mediated by the cohesin complex, is essential for chromosome segregation. The forces holding the two sisters resist the tendency of microtubules to prematurely pull sister DNAs apart and thereby prevent random segregation of the genome during mitosis, and consequent a...

journal_title：Biochemical Society transactions

authors： Oliveira RA,Nasmyth K

更新日期：2010-12-01 00:00:00

abstract：:The cytochromes P450 (P450s) are a superfamily of oxidative haemoproteins that are capable of catalysing a vast range of oxidative transformations, including the oxidation of unactivated alkanes, often with high stereo- and regio-selectivity. Fatty acid hydroxylation by P450s is widespread across both bacteria and hig...

journal_title：Biochemical Society transactions

authors： Cryle MJ

更新日期：2010-08-01 00:00:00

abstract：:The completely sequenced genome of the cyanobacterium Synechocystis PCC 6803 contains several open reading frames, of which the deduced amino acid sequences show similarities to proteins known to be involved in FeS cluster synthesis of nitrogenase (Nif proteins) and other FeS proteins (Isc proteins). In this article, ...

journal_title：Biochemical Society transactions

authors： Seidler A,Jaschkowitz K,Wollenberg M

更新日期：2001-08-01 00:00:00

abstract：:CRISPR base editing technology is a promising genome editing tool as (i) it does not require a DNA template to introduce mutations and (ii) it avoids creating DNA double-strand breaks, which can lead to unintended chromosomal alterations or elicit an unwanted DNA damage response. Given many cancers originate from poin...

journal_title：Biochemical Society transactions

authors： Pal M,Herold MJ

更新日期：2021-01-15 00:00:00

abstract：:There is increasing recognition that numerous neurodegenerative conditions have the same underlying pathogenetic mechanism, namely a change in protein conformation, where the beta-sheet content is increased. In Alzheimer's disease, amyloid deposition in the form of neuritic plaques and congophilic angiopathy is driven...

journal_title：Biochemical Society transactions

authors： Wisniewski T,Brown DR,Sigurdsson EM

更新日期：2002-08-01 00:00:00

abstract：:Glycosaminoglycans (GAGs), present in the extracellular matrix, are exploited by numerous, distinct microbes for cellular attachment, adhesion, invasion and evasion of the host immune system. Glycosaminoglycans, including the widely used, clinical anticoagulant heparin and semi-synthetic analogues thereof, have been r...

journal_title：Biochemical Society transactions

authors： Mycroft-West CJ,Yates EA,Skidmore MA

更新日期：2018-08-20 00:00:00

abstract：:A limited number of bacteria, yeast and fungi can convert hemicellulose or its monomers (xylose, arabinose, mannose and galactose) into ethanol with a satisfactory yield and productivity. In the present study we tested a number of thermophilic enrichment cultures, and new isolates of thermophilic anaerobic bacterial s...

journal_title：Biochemical Society transactions

authors： Sommer P,Georgieva T,Ahring BK

更新日期：2004-04-01 00:00:00

abstract：:The ability to permit gene expression is managed by a set of relatively well known regulatory mechanisms. Nonetheless, this property can also be acquired during a life span as a consequence of environmental stimuli. Interestingly, some acquired information can be passed to the next generation of individuals without mo...

journal_title：Biochemical Society transactions

authors： Mazaira GI,Daneri-Becerra C,Zgajnar NR,Lotufo CM,Galigniana MD

更新日期：2018-02-19 00:00:00

abstract：:Cytochrome P450 (CYP) enzymes represent a large superfamily that displays extraordinarily diverse substrate specificities. After a concise review about CYPs of the CYP1A subfamily, which plays a crucial role in procarcinogen activation, this paper presents segment-directed mutagenesis. This approach generates a librar...

journal_title：Biochemical Society transactions

authors： Urban P,Jobert AS,Lainé R,Pompon D

更新日期：2001-05-01 00:00:00

abstract：:The design and synthesis of SpmTrien (1,12-diamino-3,6,9-triazadodecane), an isosteric and charge-deficient analogue of spermine with excellent chelating properties towards Cu(2+) ions, as well as novel N(1)- and N(12)-Ac-SpmTriens and bis-Et-SpmTrien (N(1),N(12)-diethyl-1,12-diamino-3,6,9-triazadodecane) are describe...

journal_title：Biochemical Society transactions

authors： Khomutov AR,Grigorenko NA,Skuridin SG

更新日期：2007-04-01 00:00:00

abstract：:Inorganic polyphosphate (polyP) accumulates in acidocalcisomes, acidic calcium stores that have been found from bacteria to human cells. Proton pumps, such as the vacuolar proton pyrophosphatase (V-H(+)-PPase or VP1), the vacuolar proton ATPase (V-H(+)-ATPase) or both, maintain their acidity. A vacuolar transporter ch...

journal_title：Biochemical Society transactions

authors： Lander N,Cordeiro C,Huang G,Docampo R

更新日期：2016-02-01 00:00:00

abstract：:The CPC (chromosomal passenger complex) performs essential roles in the regulation and co-ordination of chromosomal and cytoskeletal events during mitosis and meiosis. The first functional analyses showed evidence of a role of the CPC in the regulation of cytokinesis. In this review, I summarize what we have learned s...

journal_title：Biochemical Society transactions

authors： Carmena M

更新日期：2008-06-01 00:00:00

abstract：:Copper chaperones, soluble copper-binding proteins, are essential for ensuring proper distribution of copper to cellular compartments and to proteins requiring copper prosthetic groups. They are found in all eukaryotic organisms. Orthologues of the three copper chaperones characterized in yeast, ATX1, CCS and COX17, a...

journal_title：Biochemical Society transactions

authors： Wintz H,Vulpe C

更新日期：2002-08-01 00:00:00