Regulation of Cdc45 in the cell cycle and after DNA damage.

abstract：:To investigate the roles of the PTEN (phosphatase and tensin homologue deleted from chromosome 10)/PI3K (phosphoinositide 3-kinase) signalling pathway in vivo, we have generated a series of mutant mice with null or tissue-specific gene-targeted deletions of Pten. Here we present our investigations of Pten function in ...

journal_title：Biochemical Society transactions

authors： Suzuki A,Sasaki T,Mak TW,Nakano T

更新日期：2004-04-01 00:00:00

abstract：:The deposition of protein aggregates in various parts of our body gives rise to several devastating diseases, and the development of probes for the selective detection of aggregated proteins is crucial to advance our understanding of the pathogenesis underlying these diseases. LCPs (luminescent conjugated polythiophen...

journal_title：Biochemical Society transactions

authors： Klingstedt T,Nilsson KP

更新日期：2012-08-01 00:00:00

abstract：:Sister-chromatid cohesion, thought to be primarily mediated by the cohesin complex, is essential for chromosome segregation. The forces holding the two sisters resist the tendency of microtubules to prematurely pull sister DNAs apart and thereby prevent random segregation of the genome during mitosis, and consequent a...

journal_title：Biochemical Society transactions

authors： Oliveira RA,Nasmyth K

更新日期：2010-12-01 00:00:00

abstract：:We study how mechanical forces integrate spatially and temporally with regulatory signals at the leading edge of migrating cells. To probe the dynamics of this system, we developed quantitative fluorescent speckle microscopy, which maps out actin cytoskeleton transport, assembly and disassembly with high spatial resol...

journal_title：Biochemical Society transactions

authors： Danuser G

更新日期：2005-12-01 00:00:00

abstract：:Bacterial hydrogenases have been harnessed to the removal of heavy metals from solution by reduction to less soluble metal species. For Pd(II), its bioreduction results in the deposition of cell-bound Pd(0)-nanoparticles that are ferromagnetic and have a high catalytic activity. Hydrogenases can also be used synthetic...

journal_title：Biochemical Society transactions

authors： Macaskie LE,Baxter-Plant VS,Creamer NJ,Humphries AC,Mikheenko IP,Mikheenko PM,Penfold DW,Yong P

更新日期：2005-02-01 00:00:00

abstract：:Crystal structure data for Escherichia coli beta-ketoacyl synthase (KAS) I with C(10) and C(12) fatty acid substrates bound in conjunction with results from mutagenizing residues in the active site leads to a model for catalysis. Differences from and similarities to the other Claisen enzymes carrying out decarboxylati...

journal_title：Biochemical Society transactions

authors： von Wettstein-Knowles P,Olsen J,Arnvig Mcguire K,Larsen S

更新日期：2000-12-01 00:00:00

abstract：:Higher eukaryotes rely on AS (alternative splicing) of pre-mRNAs (mRNA precursors) to generate more than one protein product from a single gene and to regulate mRNA stability and translational activity. An important example of the latter function involves an interplay between AS and NMD (nonsense-mediated decay), a cy...

journal_title：Biochemical Society transactions

authors： Hamid FM,Makeyev EV

更新日期：2014-08-01 00:00:00

abstract：:Genetic down-regulation of a major nutrient-sensing pathway, TOR (target of rapamycin) signalling, can improve health and extend lifespan in evolutionarily distant organisms such as yeast and mammals. Recently, it has been demonstrated that treatment with a pharmacological inhibitor of the TOR pathway, rapamycin, can ...

journal_title：Biochemical Society transactions

authors： Bjedov I,Partridge L

更新日期：2011-04-01 00:00:00

abstract：:Similar changes in glycosylation occur in the colonic epithelium in inflammatory conditions such as ulcerative colitis and Crohn's disease and also in colon cancer and precancerous adenomatous polyps. They include reduced length of O-glycans, reduced sulfation, increased sialylation and increased expression of oncofet...

journal_title：Biochemical Society transactions

authors： Rhodes JM,Campbell BJ,Yu LG

更新日期：2008-12-01 00:00:00

abstract：:The n-6 polyunsaturated fatty acid, arachidonic acid, is a precursor of prostaglandins, leukotrienes and related compounds that have important roles as mediators and regulators of inflammation. Consuming increased amounts of long chain n-3 polyunsaturated fatty acids (found in oily fish and fish oils) results in a par...

journal_title：Biochemical Society transactions

authors： Calder PC

更新日期：2005-04-01 00:00:00

abstract：:Understanding how cognitive processes including learning, memory, decision making and ideation are encoded by the genome is a key question in biology. Identification of sets of genes underlying human mental disorders is a path towards this objective. Schizophrenia is a common disease with cognitive symptoms, high heri...

journal_title：Biochemical Society transactions

authors： Tam GW,van de Lagemaat LN,Redon R,Strathdee KE,Croning MD,Malloy MP,Muir WJ,Pickard BS,Deary IJ,Blackwood DH,Carter NP,Grant SG

更新日期：2010-04-01 00:00:00

abstract：:A general strategy is described for the amplified expression, purification and characterization in Escherichia coli of multidrug efflux proteins from Staphylococcus aureus, Bacillus subtilis, Methanococcus janaschii and E. coli. They all catalyse drug/H(+) antiport of substrates such as quinolones and ethidium and exe...

journal_title：Biochemical Society transactions

authors： Henderson PJ,Hoyle CK,Ward A

更新日期：2000-01-01 00:00:00

abstract：:Shewanella oneidensis MR-1 is a sediment organism capable of dissimilatory reduction of insoluble metal compounds such as those of Fe(II) and Mn(IV). This bacterium has been used as a model organism for potential applications in bioremediation of contaminated environments and in the production of energy in microbial f...

journal_title：Biochemical Society transactions

authors： Louro RO,Paquete CM

更新日期：2012-12-01 00:00:00

abstract：:AWAP IV constitutes the C-terminal domain of the larger 81 kDa protein AWAK [Avian WAP (whey acidic protein) domain- and Kunitz domain-containing], which is predicted, through conserved domain database searching, to contain at least four WAP domains and one Kunitz domain. RT (reverse transcription)-PCR analyses reveal...

journal_title：Biochemical Society transactions

authors： Townes CL,Milona P,Hall J

更新日期：2006-04-01 00:00:00

abstract：:Activated tyrosine kinase receptors acquire ubiquitin tags. Ubiquitination governs receptor down-regulation through interaction with components of the endosomal ESCRT (endosomal sorting complexes required for transport) machinery that shepherds receptors into luminal vesicles of multivesicular bodies en route to the l...

journal_title：Biochemical Society transactions

authors： Urbé S,McCullough J,Row P,Prior IA,Welchman R,Clague MJ

更新日期：2006-11-01 00:00:00

abstract：:Activation of the BCR (B cell antigen receptor) stimulates the production of both PtdIns(3,4,5) P3 and Ins(1,4,5) P3. PtdIns(3,4,5) P3 and Ins(1,4,5) P3 are generated from a common substrate, PtdIns(4,5) P2. In some systems, continuous PtdIns(4,5) P2 synthesis is necessary for maximal Ins(1,4,5) P3 production, but whe...

journal_title：Biochemical Society transactions

authors： Carpenter CL

更新日期：2004-04-01 00:00:00

abstract：:Our first experience of mannan-binding lectin (MBL)-replacement therapy was with a patient experiencing recurrent erythema multiforme associated with reactivation of herpes simplex virus; his erythematous eruptions could be controlled with infusions of fresh frozen plasma containing MBL, but not with plasma lacking MB...

journal_title：Biochemical Society transactions

authors： Valdimarsson H

更新日期：2003-08-01 00:00:00

abstract：:Classical tissue engineering is aimed mainly at producing anatomically and physiologically realistic replacements for normal human tissues. It is done either by encouraging cellular colonization of manufactured matrices or cellular recolonization of decellularized natural extracellular matrices from donor organs, or b...

journal_title：Biochemical Society transactions

authors： Davies JA,Cachat E

更新日期：2016-06-15 00:00:00

abstract：:Glutamate transporters are essential for terminating synaptic excitation and for maintaining extracellular glutamate concentrations below neurotoxic levels. These transporters also mediate a thermodynamically uncoupled chloride flux that is activated by two of the molecules that they transport - sodium and glutamate. ...

journal_title：Biochemical Society transactions

authors： Kanner BI,Kavanaugh MP,Bendahan A

更新日期：2001-11-01 00:00:00

abstract：:Structure-based design has gained credibility as a valuable component of the modern drug discovery process. The technique of SSNMR (solid-state NMR) promises to be a useful counterpart to the conventional experimental techniques of X-ray crystallography and solution-state NMR for providing structural features of drug ...

journal_title：Biochemical Society transactions

authors： Middleton DA

更新日期：2007-11-01 00:00:00

abstract：:The mitotic spindle is the macromolecular machine utilized to accurately segregate chromosomes in cells. How this self-organized structure assembles is a key aspect of understanding spindle morphogenesis. In the present review, we focus on understanding mechanisms of spindle self-assembly and address how subcellular s...

journal_title：Biochemical Society transactions

authors： Weaver LN,Walczak CE

更新日期：2015-02-01 00:00:00

abstract：:AD (Alzheimer's disease) is a neurodegenerative disease characterized by a gradual loss of neurons and the accumulation of neurotoxic Aβ (amyloid β-peptide) and hyperphosphorylated tau. The discovery of mutations in three genes, PSEN1 (presenilin 1), PSEN2 (presenilin 2) and APP (amyloid precursor protein), in patient...

journal_title：Biochemical Society transactions

authors： Coen K,Annaert W

更新日期：2010-12-01 00:00:00

abstract：:The Ran GTPase controls many cellular functions, including nucleocytoplasmic trafficking, spindle assembly, nuclear assembly and cell-cycle progression. Considerable evidence suggests that diffusible Ran-GTP near mitotic chromatin facilitates the release of critical factors from nuclear transport receptors, thereby pr...

journal_title：Biochemical Society transactions

authors： Dasso M

更新日期：2006-11-01 00:00:00

abstract：:Polycomb group (PcG) proteins are highly conserved chromatin-modifying complexes that implement gene silencing in higher eukaryotes. Thousands of genes and multiple developmental processes are regulated by PcG proteins. As the first chromatin modifier been identified in model plant Arabidopsis thaliana, the methyltran...

journal_title：Biochemical Society transactions

authors： Jiao H,Xie Y,Li Z

更新日期：2020-08-28 00:00:00

abstract：:Many Zn(II)2Cys6 transcriptional regulators exhibit changes in phosphorylation that are coincident with their roles in transcriptional activation. It is, however, unclear whether these changes occur as a cause of, or as a result of, transcriptional activation. In this paper, we explore the relationship between these t...

journal_title：Biochemical Society transactions

authors： Leverentz MK,Reece RJ

更新日期：2006-11-01 00:00:00

abstract：:AMPK (AMP-activated protein kinase) is a cellular energy sensor that monitors the ratio of AMP/ATP, and possibly also ADP/ATP, inside cells. Once activated by falling cellular energy levels, it acts to restore energy homoeostasis by switching on catabolic pathways that generate ATP, while switching off anabolic pathwa...

journal_title：Biochemical Society transactions

authors： Gowans GJ,Hardie DG

更新日期：2014-02-01 00:00:00

abstract：:There is clear evidence implicating oxidative stress in the pathology of many different neurodegenerative diseases. ROS (reactive oxygen species) are the primary mediators of oxidative stress and many of the aggregating proteins and peptides associated with neurodegenerative disease can generate hydrogen peroxide, a k...

journal_title：Biochemical Society transactions

authors： Tabner BJ,El-Agnaf OM,German MJ,Fullwood NJ,Allsop D

更新日期：2005-11-01 00:00:00

abstract：:The network of the ER (endoplasmic reticulum) is set up by cytoskeletal control of the movement and remodelling of polygonal rings of tubules, bundles of tubules and cisternal regions. We have developed a new image analysis tool, persistency mapping, to understand the framework upon which the plant ER remodels. With t...

journal_title：Biochemical Society transactions

authors： Griffing LR

更新日期：2010-06-01 00:00:00

abstract：:Microvilli are found on the apical surface of epithelial cells. Recent studies on the microvillar proteins ezrin and EBP50 (ezrin/radixin/moesin-binding phosphoprotein of 50 kDa) have revealed both the dynamics and the regulation of microvillar components, and how a dynamic ezrin phosphocycle is necessary to confine m...

journal_title：Biochemical Society transactions

authors： Viswanatha R,Bretscher A,Garbett D

更新日期：2014-02-01 00:00:00

abstract：:Fatty acid biosynthesis is catalysed in most bacteria by a group of highly conserved proteins known as the Type II fatty acid synthase (FAS) system. The Type II system organization is distinct from its mammalian counterpart and offers several unique sites for selective inhibition by antibacterial agents. There has bee...

journal_title：Biochemical Society transactions

authors： Marrakchi H,Zhang YM,Rock CO

更新日期：2002-11-01 00:00:00