Abstract:
:Activated tyrosine kinase receptors acquire ubiquitin tags. Ubiquitination governs receptor down-regulation through interaction with components of the endosomal ESCRT (endosomal sorting complexes required for transport) machinery that shepherds receptors into luminal vesicles of multivesicular bodies en route to the lysosome. We have characterized two de-ubiquitinating enzymes that interact with components of this machinery. AMSH [associated molecule with the SH3 domain (Src homology 3 domain) of STAM (signal transducing adapter molecule)] shows specificity for Lys63- over Lys48-linked ubiquitin and may act to rescue receptors from taking the lysosomal pathway. In contrast, UBPY (ubiquitin-specific processing protease Y) does not discriminate between Lys48 and Lys63-linked chains and is required for lysosomal sorting.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Urbé S,McCullough J,Row P,Prior IA,Welchman R,Clague MJdoi
10.1042/BST0340754subject
Has Abstractpub_date
2006-11-01 00:00:00pages
754-6issue
Pt 5eissn
0300-5127issn
1470-8752pii
BST0340754journal_volume
34pub_type
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