Incorporation of iron-sulphur clusters in membrane-bound proteins.

abstract：:Single-molecule fluorescence methods, particularly single-molecule FRET (fluorescence resonance energy transfer), have provided novel insights into the structure, interactions and dynamics of biological systems. ALEX (alternating-laser excitation) spectroscopy is a new method that extends single-molecule FRET by provi...

journal_title：Biochemical Society transactions

authors： Santoso Y,Hwang LC,Le Reste L,Kapanidis AN

更新日期：2008-08-01 00:00:00

abstract：:The endoplasmic reticulum is traditionally perceived as the key compartment for regulating intracellular cholesterol metabolism. Increasing evidence suggests that the endocytic pathway provides an additional regulatory level governing intracellular cholesterol trafficking and homoeostasis. Sterols can enter, and appar...

journal_title：Biochemical Society transactions

authors： Hölttä-Vuori M,Ikonen E

更新日期：2006-06-01 00:00:00

abstract：:Anaphase B spindle elongation plays an important role in chromosome segregation. In the present paper, we discuss our model for anaphase B in Drosophila syncytial embryos, in which spindle elongation depends on an ip (interpolar) MT (microtubule) sliding filament mechanism generated by homotetrameric kinesin-5 motors ...

journal_title：Biochemical Society transactions

authors： Brust-Mascher I,Scholey JM

更新日期：2011-10-01 00:00:00

abstract：:Sugars that are readily respired by plant cells inhibit greening when dO2 and mu are relatively high. As sugar concentration in the batch culture medium is depleted and mu declines, greening is permitted. The mechanism of this effect has not been satisfactorily explained. ...

journal_title：Biochemical Society transactions

authors： Dalton C

更新日期：1980-08-01 00:00:00

abstract：:CD spectroscopy is an established and valuable technique for examining protein structure, dynamics and folding. Because of its ability to sensitively detect conformational changes, it has important potential for drug discovery, enabling screening for ligand and drug binding, and detection of potential candidates for n...

journal_title：Biochemical Society transactions

authors： Wallace BA,Janes RW

更新日期：2003-06-01 00:00:00

abstract：:Experimental processes in the life sciences are becoming increasingly complex. As a result, recording, archiving and sharing descriptions of these processes and of the results of experiments is becoming ever more challenging. However, validation of results, sharing of best practice and integrated analysis all require ...

journal_title：Biochemical Society transactions

authors： Paton NW

更新日期：2008-02-01 00:00:00

abstract：:Plant chloroplasts contain an intricate photosynthetic membrane system, the thylakoids, and are surrounded by two envelope membranes at which thylakoid lipids are assembled. The glycoglycerolipids mono- and digalactosyldiacylglycerol, and sulfoquinovosyldiacylglycerol as well as phosphatidylglycerol, are present in th...

journal_title：Biochemical Society transactions

authors： Wang Z,Benning C

更新日期：2012-04-01 00:00:00

abstract：:Intracellular cross-talk or synergism between receptors coupled negatively to adenylate cyclase via PTX-sensitive Gi-protein(s) and receptors coupled to phospholipase C via PTX-insensitive G-proteins is prevalent in a variety of cell types (see above) and may represent a universal mechanism for the modulation of phosp...

journal_title：Biochemical Society transactions

authors： Dickenson JM,Hill SJ

更新日期：1993-11-01 00:00:00

abstract：:Classical tissue engineering is aimed mainly at producing anatomically and physiologically realistic replacements for normal human tissues. It is done either by encouraging cellular colonization of manufactured matrices or cellular recolonization of decellularized natural extracellular matrices from donor organs, or b...

journal_title：Biochemical Society transactions

authors： Davies JA,Cachat E

更新日期：2016-06-15 00:00:00

abstract：:Cultured pluripotent stem cells hold great promise for regenerative medicine. Considerable efforts have been invested into the refinement and definition of improved culture systems that sustain self-renewal and avoid differentiation of pluripotent cells in vitro. Recent studies have, however, found that the choice of ...

journal_title：Biochemical Society transactions

authors： McEwen KR,Leitch HG,Amouroux R,Hajkova P

更新日期：2013-06-01 00:00:00

abstract：:Co-ordinated myocyte handling of calcium is essential for efficient excitation-contraction coupling in the heart. The calcium cycling activity can be modulated by adrenergic stimulation and subsequent phosphorylation. Important functional consequences of phosphorylation include a greater influx of calcium through the ...

journal_title：Biochemical Society transactions

authors： Lygren B,Taskén K

更新日期：2006-08-01 00:00:00

abstract：:Mutations activating the PI3K (phosphoinositide 3-kinase)/Akt signalling pathway and inactivating the TP53 tumour-suppressor gene are common mechanisms that cancer cells require to proliferate and escape pre-programmed cell death. In a well-described mechanism, Akt mediates negative control of p53 levels through enhan...

journal_title：Biochemical Society transactions

authors： Abraham AG,O'Neill E

更新日期：2014-08-01 00:00:00

abstract：:Recent findings that some 24 inherited diseases and anomalies are caused by defects in proteins of the NE (nuclear envelope) and lamina have resulted in a fundamental reassessment of the functions of the NE and underlying lamina. Instead of just regarding the NE and lamina as a molecular filtering device, regulating t...

journal_title：Biochemical Society transactions

authors： Cohen TV,Hernandez L,Stewart CL

更新日期：2008-12-01 00:00:00

abstract：:The cytochromes P450 (P450s) are a superfamily of oxidative haemoproteins that are capable of catalysing a vast range of oxidative transformations, including the oxidation of unactivated alkanes, often with high stereo- and regio-selectivity. Fatty acid hydroxylation by P450s is widespread across both bacteria and hig...

journal_title：Biochemical Society transactions

authors： Cryle MJ

更新日期：2010-08-01 00:00:00

abstract：:We have investigated the cellular distribution of p122RhoGAP, a GTPase-activating protein of Rho small GTPase and an activator of phospholipase C-delta(1). Immunofluorescence studies demonstrated that endogenous p122 is localized at the tips of actin stress fibres and co-localizes with vinculin in normal rat kidney ce...

journal_title：Biochemical Society transactions

authors： Kawai K,Yamaga M,Iwamae Y,Kiyota M,Kamata H,Hirata H,Homma Y,Yagisawa H

更新日期：2004-12-01 00:00:00

abstract：:Recent studies of some proteins involved in the formation of focal adhesions are described. These include fibronectin, integrins, talin, Dok1 and filamin. Emphasis is placed on features that facilitate regulated assembly of complexes; these include a modular construction and flexible regions that provide interaction s...

journal_title：Biochemical Society transactions

authors： Campbell ID

更新日期：2008-04-01 00:00:00

abstract：:The physiological roles of insulin and insulin-like growth factors (IGFs) are distinct, with insulin acting to regulate cellular uptake and metabolism of fuels, whereas IGFs promote cell growth, survival and differentiation. The only components of signalling pathways known to be unique to insulin and IGFs are their re...

journal_title：Biochemical Society transactions

authors： Siddle K,Ursø B,Niesler CA,Cope DL,Molina L,Surinya KH,Soos MA

更新日期：2001-08-01 00:00:00

abstract：:Reactive oxygen species (ROS) were originally thought to only be released by phagocytic cells during their role in host defence. It is now clear that ROS have a cell signalling role in many biological systems, both in animals and in plants. ROS induce programmed cell death or necrosis, induce or suppress the expressio...

journal_title：Biochemical Society transactions

authors： Hancock JT,Desikan R,Neill SJ

更新日期：2001-05-01 00:00:00

abstract：:Glyoxals are reactive alpha-oxoaldehydes that are formed endogenously from sugars, the levels of which are increased in various pathological conditions associated with hyperglycaemia and thiamine deficiency. However, the molecular cytotoxic mechanisms of glyoxal are not known. Results presented here and in the other s...

journal_title：Biochemical Society transactions

authors： Shangari N,Bruce WR,Poon R,O'Brien PJ

更新日期：2003-12-01 00:00:00

abstract：:[Ca(2+)](i) (intracellular Ca(2+) concentration) oscillations play a central role in the activation of T-lymphocytes by antigen. Oscillations in T-cells are absolutely dependent on Ca(2+) influx through store-operated CRAC channels (Ca(2+)-release-activated Ca(2+) channels), and evidence suggests that they arise from ...

journal_title：Biochemical Society transactions

authors： Lewis RS

更新日期：2003-10-01 00:00:00

abstract：:The identification of nitric oxide-bound leghaemoglobin within soya bean nodules has led to the question of how Bradyrhizobium japonicum bacteroids overcome the toxicity of this nitric oxide. It has previously been shown that one candidate for nitric oxide detoxification, the respiratory nitric oxide reductase, is exp...

journal_title：Biochemical Society transactions

authors： Meakin GE,Jepson BJ,Richardson DJ,Bedmar EJ,Delgado MJ

更新日期：2006-02-01 00:00:00

abstract：:Studies on those enzymes and electron-transfer proteins involved in the catabolism of 'C1' substrates in methylotrophic bacteria have provided a wealth of information concerning the transfer of electrons and hydrogen by quantum tunnelling mechanisms. With regard to H-transfer, studies with MADH have provided the first...

journal_title：Biochemical Society transactions

authors： Scrutton NS

更新日期：1999-12-01 00:00:00

abstract：:The intestinal epithelium represents one of our most important interfaces with the external environment. It must remain tightly balanced to allow nutrient absorption, but maintain barrier function and immune homoeostasis, a failure of which results in chronic infection or debilitating inflammatory bowel disease (IBD)....

journal_title：Biochemical Society transactions

authors： Worthington JJ

更新日期：2015-08-01 00:00:00

abstract：:Evidence indicates that leucine-rich repeat kinase 2 (LRRK2) controls multiple processes in neurons and glia cells. Deregulated LRRK2 activity due to gene mutation represents the most common cause of autosomal dominant Parkinson's disease (PD). Protein kinase A (PKA)-mediated signaling is a key regulator of brain func...

journal_title：Biochemical Society transactions

authors： Greggio E,Bubacco L,Russo I

更新日期：2017-02-08 00:00:00

abstract：:We have shown that yeast mutants with defects in the Ada adaptor proteins are defective in hormone-dependent gene activation by ectopically expressed human glucocorticoid receptor (GR). Others have shown that the Ada2 protein is required for physical interactions between some activation domains and TBP (TATA-binding p...

journal_title：Biochemical Society transactions

authors： Wallberg AE,Flinn EM,Gustafsson JA,Wright AP

更新日期：2000-01-01 00:00:00

abstract：:Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are the most common cause of familial Parkinson's disease (PD) with autosomal dominant inheritance. Accordingly, LRRK2 has emerged as a promising therapeutic target for disease modification in PD. Since the first discovery of LRRK2 mutations some 12 years ago,...

journal_title：Biochemical Society transactions

authors： Islam MS,Moore DJ

更新日期：2017-02-08 00:00:00

abstract：:We have described a large number of different mutations in the LPL gene that result in completely catalytically defective LPL protein. More recently exonic polymorphisms in the LPL gene have been described that do not result in the catalytic activity of LPL being significantly impaired. Furthermore we have recently de...

journal_title：Biochemical Society transactions

authors： Hayden MR,Kastelein JJ,Funke H,Brunzell JD,Ma Y

更新日期：1993-05-01 00:00:00

abstract：:An extraordinary array of P450 (cytochrome P450) enzymes are encoded on the genome of the human pathogen Mycobacterium tuberculosis (Mtb) and in related mycobacteria and actinobacteria. These include the first characterized sterol 14alpha-demethylase P450 (CYP51), a known target for azole and triazole drugs in yeasts ...

journal_title：Biochemical Society transactions

authors： McLean KJ,Dunford AJ,Sabri M,Neeli R,Girvan HM,Balding PR,Leys D,Seward HE,Marshall KR,Munro AW

更新日期：2006-12-01 00:00:00

abstract：:Saccharomyces cerevisiae cells grown in glucose have larger average size than cells grown in ethanol. Besides, yeast must reach a carbon source-modulated critical cell size in order to enter S phase at Start. This control is of outmost physiological relevance, since it allows us to coordinate cell growth with cell cyc...

journal_title：Biochemical Society transactions

authors： Vanoni M,Rossi RL,Querin L,Zinzalla V,Alberghina L

更新日期：2005-02-01 00:00:00

abstract：:Membrane proteins are drug targets for a wide range of diseases. Having access to appropriate samples for further research underpins the pharmaceutical industry's strategy for developing new drugs. This is typically achieved by synthesizing a protein of interest in host cells that can be cultured on a large scale, all...

journal_title：Biochemical Society transactions

authors： Bawa Z,Bland CE,Bonander N,Bora N,Cartwright SP,Clare M,Conner MT,Darby RA,Dilworth MV,Holmes WJ,Jamshad M,Routledge SJ,Gross SR,Bill RM

更新日期：2011-06-01 00:00:00