Abstract:
:Membrane proteins are drug targets for a wide range of diseases. Having access to appropriate samples for further research underpins the pharmaceutical industry's strategy for developing new drugs. This is typically achieved by synthesizing a protein of interest in host cells that can be cultured on a large scale, allowing the isolation of the pure protein in quantities much higher than those found in the protein's native source. Yeast is a popular host as it is a eukaryote with similar synthetic machinery to that of the native human source cells of many proteins of interest, while also being quick, easy and cheap to grow and process. Even in these cells, the production of human membrane proteins can be plagued by low functional yields; we wish to understand why. We have identified molecular mechanisms and culture parameters underpinning high yields and have consolidated our findings to engineer improved yeast host strains. By relieving the bottlenecks to recombinant membrane protein production in yeast, we aim to contribute to the drug discovery pipeline, while providing insight into translational processes.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Bawa Z,Bland CE,Bonander N,Bora N,Cartwright SP,Clare M,Conner MT,Darby RA,Dilworth MV,Holmes WJ,Jamshad M,Routledge SJ,Gross SR,Bill RMdoi
10.1042/BST0390719subject
Has Abstractpub_date
2011-06-01 00:00:00pages
719-23issue
3eissn
0300-5127issn
1470-8752pii
BST0390719journal_volume
39pub_type
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