Abstract:
:Saccharomyces cerevisiae cells grown in glucose have larger average size than cells grown in ethanol. Besides, yeast must reach a carbon source-modulated critical cell size in order to enter S phase at Start. This control is of outmost physiological relevance, since it allows us to coordinate cell growth with cell cycle progression and it is responsible for cell size homeostasis. The cell sizer mechanism requires the overcoming of two sequential thresholds, involving Cln3 and Far1, and Clb5,6 and Sic1, respectively. When both thresholds are non-functional, carbon source modulation of cell size at Start is completely abolished. Since inactivation of extracellular glucose sensing through deletion of either the GPR1 or the GPA2 gene causes a marked, but partial, reduction in the ability to modulate cell size and protein content at Start, it is proposed that both extracellular and intracellular glucose signalling is required for properly setting the cell sizer in glucose media.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Vanoni M,Rossi RL,Querin L,Zinzalla V,Alberghina Ldoi
10.1042/BST0330294keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
294-6issue
Pt 1eissn
0300-5127issn
1470-8752pii
BST0330294journal_volume
33pub_type
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