Mitotic motors and chromosome segregation: the mechanism of anaphase B.

Abstract:

:Anaphase B spindle elongation plays an important role in chromosome segregation. In the present paper, we discuss our model for anaphase B in Drosophila syncytial embryos, in which spindle elongation depends on an ip (interpolar) MT (microtubule) sliding filament mechanism generated by homotetrameric kinesin-5 motors acting in concert with poleward ipMT flux, which acts as an 'on/off' switch. Specifically, the pre-anaphase B spindle is maintained at a steady-state length by the balance between ipMT sliding and ipMT depolymerization at spindle poles, producing poleward flux. Cyclin B degradation at anaphase B onset triggers: (i) an MT catastrophe gradient causing ipMT plus ends to invade the overlap zone where ipMT sliding forces are generated; and (ii) the inhibition of ipMT minus-end depolymerization so flux is turned 'off', tipping the balance of forces to allow outward ipMT sliding to push apart the spindle poles. We briefly comment on the relationship of this model to anaphase B in other systems.

journal_name

Biochem Soc Trans

authors

Brust-Mascher I,Scholey JM

doi

10.1042/BST0391149

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

1149-53

issue

5

eissn

0300-5127

issn

1470-8752

pii

BST0391149

journal_volume

39

pub_type

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