Molecular cloning, polymorphism and association analyses of a novel porcine mRNA differentially expressed in the Longissimus muscle tissues from Meishan and Large White pigs.

Abstract:

:The mRNA differential display technique was performed to investigate the differences of gene expression in the longissimus muscle tissues from Meishan and Large White pigs. One novel mRNA that was differentially expressed was identified through semi-quantitative RT-PCR and the cDNA complete sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. The nucleotide sequence of the mRNA is not homologous to any of the known porcine genes. Sequence prediction analysis revealed that the this mRNA is not protein-coding mRNA. Polymorphism analyses revealed that there was a C-T mutation on the position of 669 bp and PCR -Dra I-RFLP analyses revealed that Chinese indigenous pig breeds and exotic pig breeds displayed obvious genotype and allele frequency differences at this locus. Association analyses revealed that this polymorphic locus was significantly associated with the drip loss rate, skin percentage, meat color value (m.Longissimus Dorsi, LD), loin eye width, loin eye area, water holding capacity, carcass length, caul fat weight, intramuscular fat (m.Longissimus Dorsi, LD), lean meat weight, lean meat percentage, backfat thickness at buttock (P < 0.05).

journal_name

Mol Biol Rep

authors

Liu GY,Xiong YZ

doi

10.1007/s11033-008-9326-0

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

1393-8

issue

6

eissn

0301-4851

issn

1573-4978

journal_volume

36

pub_type

杂志文章
  • Technetium-99m labeled Ibuprofen: Development and biological evaluation using sterile inflammation induced animal models.

    abstract::In this study we are presenting the development of technetium-99m (99mTc) labeled ibuprofen for the imaging of aseptic inflammation. 99mTc-Ibuprofen complex was developed by optimizing the radiolabeling conditions such as reaction time, ligand and reducing agent concentration, pH, reaction time and temperature. Follow...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04762-2

    authors: Khan NU,Naqvi SAR,Sohail H,Roohi S,Jamal MA

    更新日期:2019-06-01 00:00:00

  • Resistance to Chilo infuscatellus (Lepidoptera: Pyraloidea) in transgenic lines of sugarcane expressing Bacillus thuringiensis derived Vip3A protein.

    abstract::Sustainable agriculture requires management of insect pests through resistance development. The biological potential of Cry toxins and Vip protein, derived from Bacillus species, is widely recognized in this context. The identification, evaluation of new insecticidal protein genes with different mode of action and ent...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-020-05355-0

    authors: Riaz S,Nasir IA,Bhatti MU,Adeyinka OS,Toufiq N,Yousaf I,Tabassum B

    更新日期:2020-04-01 00:00:00

  • Identification of candidate genes associated with CBB resistance in common bean HR45 (Phaseolus vulgaris L.) using cDNA-AFLP.

    abstract::Common bacterial blight (CBB), incited by Xanthomonas axonopodis pv. phaseoli (Xap), is a serious seed-borne disease of common bean (Phaseolus vulgaris L.) in both temperate and tropical production zones. The line HR45 is highly resistant to Xap infection on leaves and pods in both field and greenhouse. To understand ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0079-1

    authors: Shi C,Chaudhary S,Yu K,Park SJ,Navabi A,McClean PE

    更新日期:2011-01-01 00:00:00

  • Matrix metalloproteinase-3 predicts clinical cardiovascular outcomes in patients with coronary artery disease: a 5 years cohort study.

    abstract::Matrix metalloproteinases (MMPs) are implicated in atherosclerosis evolution into a coronary artery disease (CAD). They could be used as biomarkers for a predictive approach when they are studied simultaneously. We aim in our study to demonstrate prospectively in patients with history of CAD that MMPs level is linked ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04914-4

    authors: Guizani I,Zidi W,Zayani Y,Boudiche S,Hadj-Taieb S,Sanhaji H,Zaroui A,Mechmeche R,Mourali MS,Feki M,Allal-Elasmi M

    更新日期:2019-10-01 00:00:00

  • Phosphorylation of proteasomes in mammalian cells.

    abstract::20S proteasomes are large (700 kDa) proteinase complexes which form the central catalytic core of larger complexes (26S proteasomes or PA28-20S complexes) formed by association with regulatory particles. These larger complexes are involved in diverse regulatory processes in the cell including cyclin breakdown, proteol...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1023/a:1006969517958

    authors: Bose S,Mason GG,Rivett AJ

    更新日期:1999-04-01 00:00:00

  • Molecular insight into the metabolic activities of a protein-rich micro alga, Arthrospira platensis by de novo transcriptome analysis.

    abstract::To gain genetic insights into the protein-rich microalga, the transcriptome of Arthrospira platensis was sequenced using Illumina technology and de novo assembly was carried out. A total of 6023 transcripts were present in the transcriptome among which 4616 transcripts were annotated with specific functions. Gene onto...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4229-1

    authors: Kumaresan V,Sannasimuthu A,Arasu MV,Al-Dhabi NA,Arockiaraj J

    更新日期:2018-10-01 00:00:00

  • Overexpressed in colorectal carcinoma gene (OCC-1) upregulation and APPL2 gene downregulation in breast cancer specimens.

    abstract::Breast cancer is the most common cancer type and the second cause of cancer death in women. Different mechanisms are contributed to the initiation and progression of the breast cancer. OCC-1 and APPL2 neighboring genes located in 12q.23.3 human chromosome region are related to colorectal cancer. Here, we intended to i...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4336-z

    authors: Ghalaei A,Kay M,Zarrinfam S,Hoseinpour P,Behmanesh M,Soltani BM

    更新日期:2018-12-01 00:00:00

  • Chromosome breakages associated with 45S ribosomal DNA sequences in spotted snakehead fish Channa punctatus.

    abstract::It is well known that transcriptionally inactive rRNA genes are correlated with DNA hyper-methylation and histone hypo-methylation and there is clear evidence in humans that DNA and histone modification which alter chromatin structure are related to chromosome fragility. Very little is known about the biological cause...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2112-z

    authors: Singh M,Barman AS

    更新日期:2013-01-01 00:00:00

  • Mammalian apoptosis-inducing protein, HAP, induces bacterial cell death.

    abstract::In attempting to produce the HAP, endoplasmic reticulum (ER) targeted apoptosis-inducing protein, as a GST-fusion protein we found that the expression of HAP, but not GST alone, induced bacterial cell death. The HAP protein inhibited the bacterial growth within 30 min after inducting HAP expression. The transmission e...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1023/b:mole.0000043551.84883.c8

    authors: Gan M,Qi Y,Wan Q,Kuang E,Liu Q,Liu X

    更新日期:2004-09-01 00:00:00

  • Comparison of the novel dipstick DNA extraction technique with two established techniques for use in biological barcoding.

    abstract::The novel dipstick DNA extraction method was tested for its reliability and usability for biological barcoding in comparison to a commercial kit and to a simplified isopropanol precipitation method using crayfish gill tissue. Following DNA extraction, the mitochondrial COI-gene was amplified in a PCR-reaction using a ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-05083-0

    authors: Martello A,Lambert B,Johnston C,Cutler J,Stumpf CF

    更新日期:2019-12-01 00:00:00

  • Development of fast and sensitive protocols for the detection of viral pathogens using a small portable convection PCR platform.

    abstract::One of the most crucial steps for preventing viral pandemics is the early detection of the causative virus on site. Various molecular and immunological approaches have been developed for virus detection. In this study, we investigated the utility of the recently introduced convection polymerase chain reaction (cPCR) p...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04961-x

    authors: Lee MH,Song KY,Hwang HJ,Kim JH,Hwang I

    更新日期:2019-10-01 00:00:00

  • Staphylococcus aureus mobile genetic elements.

    abstract::Among the bacteria groups, most of them are known to be beneficial to human being whereas only a minority is being recognized as harmful. The pathogenicity of bacteria is due, in part, to their rapid adaptation in the presence of selective pressures exerted by the human host. In addition, through their genomes, bacter...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1007/s11033-014-3367-3

    authors: Alibayov B,Baba-Moussa L,Sina H,Zdeňková K,Demnerová K

    更新日期:2014-08-01 00:00:00

  • Methylenetetrahydrofolate reductase gene polymorphism in diabetes and obesity.

    abstract::Methylenetetrahydrofolate reductase (MTHFR) polymorphism may play an important role in the pathophysiology of obesity and diabetes accompanied by obesity due to its influence on plasma homocysteine levels. There are significant and sometimes very strong relationship between levels of homocysteine and several multi-sys...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-009-9545-z

    authors: Tavakkoly Bazzaz J,Shojapoor M,Nazem H,Amiri P,Fakhrzadeh H,Heshmat R,Parvizi M,Hasani Ranjbar S,Amoli MM

    更新日期:2010-01-01 00:00:00

  • Rapid assessment of protein-tyrosine phosphatase expression levels by RT-PCR with degenerate primers.

    abstract::Using degenerate oligodeoxynucleotide primers we previously obtained cDNA fragments from ten different murine protein-tyrosine phosphatases (PTPases). Employing this same primer set, a method was developed to assess the expression levels of these PTPase family members in a fast and simple way. RT-PCR products of sever...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00997155

    authors: Hendriks W,Brugman C,Schepens J,Wieringa B

    更新日期:1994-03-01 00:00:00

  • Isolation of microsatellite loci in the African tree species Staudtia kamerunensis (Myristicaceae) using high-throughput sequencing.

    abstract::Staudtia kamerunensis (Myristicaceae) or 'Niové' is an evergreen tree widespread in Central African moist forests. The bark and seeds are used in traditional medicine, yet the tree is mainly harvested for its high quality, multi-purpose timber. To facilitate sustainable harvesting and conservation of the species, we a...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-018-4239-z

    authors: Vanden Abeele S,Hardy OJ,Janssens SB

    更新日期:2018-10-01 00:00:00

  • Interaction between lysine-rich histones and DNA.

    abstract::Using a membrane filter retention technique we have studied the interaction between DNA and lysine rich histone H5 in vitro. It is found that, depending on the ionic conditions, H5 can bind DNA in a random or cooperative manner and exhibits a preference to DNA with high molecular weight and/or high A + T content, as a...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00775749

    authors: Iovcheva C,Dessev GN

    更新日期:1980-03-31 00:00:00

  • Protective roles of quercetin in acute myocardial ischemia and reperfusion injury in rats.

    abstract::Myocardial ischemia and reperfusion (MI/R) is associated with an intense inflammatory reaction, which may lead to myocyte injury. In this study, we investigated the effect of quercetin, an inhibitor of c-Jun N-terminal kinase on ischemia/reperfusion injury in isolated rat hearts. Rat models of MI/R were induced by cor...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2002-4

    authors: Jin HB,Yang YB,Song YL,Zhang YC,Li YR

    更新日期:2012-12-01 00:00:00

  • Characterization of a novel splicing variant of KLHL5, a member of the kelch protein family.

    abstract::The kelch-repeat protein family is a recently found new kind of actin-binding protein. It is characterized by tandemly arranged motifs of about 50 amino acids. Previous study showed that most members of the kelch-repeat family were cytoskeletal proteins implicated in various cellular processes, such as actin cytoskele...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1023/a:1026372901766

    authors: Xu J,Gu S,Wang S,Dai J,Ji C,Jin Y,Qian J,Wang L,Ye X,Xie Y,Mao Y

    更新日期:2003-12-01 00:00:00

  • Mesenchymal stem cell-based therapy for autoimmune diseases: emerging roles of extracellular vesicles.

    abstract::In autoimmune disease body's own immune system knows healthy cells as undesired and foreign cells. Over 80 types of autoimmune diseases have been recognized. Currently, at clinical practice, treatment strategies for autoimmune disorders are based on relieving symptoms and preventing difficulties. In other words, there...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,评审

    doi:10.1007/s11033-019-04588-y

    authors: Rad F,Ghorbani M,Mohammadi Roushandeh A,Habibi Roudkenar M

    更新日期:2019-02-01 00:00:00

  • Molecular character of a phosphatase 2C (PP2C) gene relation to stress tolerance in Arabidopsis thaliana.

    abstract::Protein phosphatases type 2C (PP2Cs) from group A, which includes the ABI1/HAB1 and PP2CA branches, are key negative regulators of ABA signaling. HAI-1 gene had been shown to affect both seed and vegetative responses to ABA, which is one of PP2Cs clade A in Arabidopsis thaliana. Transgenic plants containing pHAI-1::GU...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2350-0

    authors: Zhang J,Li X,He Z,Zhao X,Wang Q,Zhou B,Yu D,Huang X,Tang D,Guo X,Liu X

    更新日期:2013-03-01 00:00:00

  • Effect of BRAF V600E mutation on tumor response of anti-EGFR monoclonal antibodies for first-line metastatic colorectal cancer treatment: a meta-analysis of randomized studies.

    abstract::Anti-EGFR monoclonal antibodies (anti-EGFR MoAbs) in metastatic colorectal cancer (mCRC) treatment are still not effective in all patients. This study aimed to evaluate the relationship between BRAF V600E mutation and the tumor response of anti-EGFR MoAbs for first-line treatment in mCRC patients. We searched the MEDL...

    journal_title:Molecular biology reports

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11033-013-2974-8

    authors: Cui D,Cao D,Yang Y,Qiu M,Huang Y,Yi C

    更新日期:2014-03-01 00:00:00

  • The targets of 2,2',5,5'-tetrachlorobiphenyl in the respiratory chain of rat liver mitochondria revealed by modular kinetic analysis.

    abstract::The response of the respiratory subsystem of oxidative phosphorylation to the environmental pollutant, 2,2',5,5'-tetrachlorobiphenyl (2,2',5,5'-TCB) was investigated by modular kinetic approach. The effects of 20 microM 2,2',5,5'-TCB on the activity of the respiratory chain modules in rat liver mitochondria oxidizing ...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1023/a:1020394016372

    authors: Mildaziene V,Nauciene Z,Krab K

    更新日期:2002-01-01 00:00:00

  • Comparative properties of rat liver chromatin in situ and in vitro at early stages after partial hepatectomy.

    abstract::The binding of acridine orange and ethidium bromide to rat liver chromatin increases by 30% one hour after partial hepatectomy, returns to the control level by the second hour and increases again by the sixth hour. The changes described were found in investigations carried out on whole cells, on isolated nuclei, and o...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/BF00777513

    authors: Sondore OY,Fedoseeva GE,Kadyckov VA,Zelenin AV

    更新日期:1978-10-16 00:00:00

  • Correlation analysis between three novel SNPs of the Src gene in bovine and milk production traits.

    abstract::As an essential signaling modulator, Src gene appears to be necessary for increased expression of the prolactin receptor, normal downstream signaling, and alveolar cell organization. In this study, we detected the polymorphism of Src gene by polymerase chain reaction-restriction fragment length polymorphism assay (PCR...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0031-4

    authors: Liu W,Wang J,Li Q,Ju Z,Huang J,Wang H,Liu S,Li J,Zhong J,Wang C

    更新日期:2010-12-01 00:00:00

  • Genetic divergence in wild population of Labeo rohita (Hamilton, 1822) from nine Indian rivers, analyzed through MtDNA cytochrome b region.

    abstract::The present study examined partial cytochrome b gene sequence of mitochondrial DNA for polymorphism and its suitability to determine the genetic differentiation in wild Labeo rohita. The 146 samples of L. rohita were collected from nine distant rivers; Satluj, Brahmaputra, Son, Chambal Mahanadi, Rapti, Chauka, Bhagira...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-011-1140-4

    authors: Luhariya RK,Lal KK,Singh RK,Mohindra V,Punia P,Chauhan UK,Gupta A,Lakra WS

    更新日期:2012-04-01 00:00:00

  • Genetic variants in metabolizing genes NQO1, NQO2, MTHFR and risk of prostate cancer: a study from North India.

    abstract::Quinone oxidoreductases (NAD(P)H): quinone oxidoreductase 1 (NQO1) and NRH: quinone oxidoreductase 2 (NQO2) are an antioxidant enzyme, important in the detoxification of environmental carcinogens. Methylene-tetra-hydrofolate reductase (MTHFR), plays a role in folate metabolism and may have oncogenic role through disru...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-012-2023-z

    authors: Mandal RK,Nissar K,Mittal RD

    更新日期:2012-12-01 00:00:00

  • Selective interactions of hnRNP M isoforms with the TET proteins TAF15 and TLS/FUS.

    abstract::The molecular composition of macromolecular assemblies engaged in transcription and splicing influences biogenesis of mRNA transcripts. Preference for one over the other interactive protein partner within those complexes is expected to change the gene expression pattern and to affect subsequent cellular events. We rep...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-014-3128-3

    authors: Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

    更新日期:2014-01-01 00:00:00

  • Adriamycin induces myocardium apoptosis through activation of nuclear factor kappaB in rat.

    abstract::Adriamycin is one of the most effective and useful antineoplastic agents. Acute doxorubicin cardiotoxicity involved cardiomyocyte apoptosis. In this study, we investigated whether adriamycin induced myocardium apoptosis through activation of nuclear factor kappaB in rat. Forty male Wistar rats were randomly divided in...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-007-9112-4

    authors: Li S,E M,Yu B

    更新日期:2008-12-01 00:00:00

  • 3-Deoxyglucosone interferes with insulin signaling and attenuates insulin action on glucose-induced GLP-1 secretion in the enteroendocrine L cell line STC-1.

    abstract::Maintenance of glucose homeostasis is reciprocally regulated by insulin and glucagon-like peptide-1 (GLP-1). We previously reported that GLP-1 secretion in response to an oral glucose load was impaired following an administration of 3-deoxyglucosone (3DG), an independent factor associated with the development of pre-d...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-019-04926-0

    authors: Song X,Zhou L,Xu H,Wang F,Liang G,Zhang L,Huang F,Jiang G

    更新日期:2019-10-01 00:00:00

  • Aucubin modulates Bcl-2 family proteins expression and inhibits caspases cascade in H₂O₂-induced PC12 cells.

    abstract::In this study, the effect of aucubin on H(2)O(2)-induced apoptosis was studied by using a rat pheochromocytoma (PC12) cell line. We have analyzed the apoptosis of H(2)O(2)-induced PC12 cells, H(2)O(2)-induced apoptosis appeared to correlate with lower Bcl-2 expression, higher Bax expression and sequential activation o...

    journal_title:Molecular biology reports

    pub_type: 杂志文章

    doi:10.1007/s11033-010-0466-7

    authors: Xue HY,Niu DY,Gao GZ,Lin QY,Jin LJ,Xu YP

    更新日期:2011-06-01 00:00:00