Abstract:
:Protein phosphatases type 2C (PP2Cs) from group A, which includes the ABI1/HAB1 and PP2CA branches, are key negative regulators of ABA signaling. HAI-1 gene had been shown to affect both seed and vegetative responses to ABA, which is one of PP2Cs clade A in Arabidopsis thaliana. Transgenic plants containing pHAI-1::GUS (β-glucuronidase) displayed GUS activity existing in the vascular system of leave veins, stems and petioles. Green fluorescent protein fused HAI-1 (HAI-1-GFP) was found in the nucleus through transient transformation assays with onion epidermal cells. The water-loss assays indicated the loss-of-function mutants did not show symptoms of wilting and they had still turgid green rosette leaves. The assays of seed germination by exogenous ABA and NaCl manifested that the loss-of-function mutants displayed higher insensitivity than wild-type plants. Taken together, the final results suggest that the HAI-1 (AT5G59220) encoded a nuclear protein and it can be highly induced by ABA and wound in Arabidposis, the stress-tolerance phenotype showed a slightly improvement when HAI-1 gene was disrupted.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Zhang J,Li X,He Z,Zhao X,Wang Q,Zhou B,Yu D,Huang X,Tang D,Guo X,Liu Xdoi
10.1007/s11033-012-2350-0subject
Has Abstractpub_date
2013-03-01 00:00:00pages
2633-44issue
3eissn
0301-4851issn
1573-4978journal_volume
40pub_type
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