Abstract:
:The human COX-2 promoter contains a direct repeat 1 (DR1) which was shown to confer responsiveness to PPARs. We found that in AN(3)CA and F9 cells, this hCOX-2 DR1 mediates responsiveness to all-trans-retinoic acid (tRA) or 9-cis-retinoic acid (9cRA), but this effect was suppressed by PPARδ. Truncated PPARδ lacking the activation domain AF2 cannot suppress RA-induced activation of the hCOX-2 gene via DR1, suggesting that cofactor recruitment by AF2 is required for the suppression by PPARδ. Gel shift assay showed that PPAR/RXR, RARβ/RXR, and RXR/RXR, bind to hCOX-2 DR1, revealing the promiscuity of this DR1. Particularly, RXR homodimer was able to bind to this DR1 only in the presence of 9cRA. Our results established that tRA and 9cRA are potent inducers of hCOX-2 and that the hCOX-2 DR1 could either serve as RARE or RXRE depending on cellular contexts.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Han K,Moon I,Lim HJdoi
10.1007/s11033-010-0173-4subject
Has Abstractpub_date
2011-02-01 00:00:00pages
833-40issue
2eissn
0301-4851issn
1573-4978journal_volume
38pub_type
杂志文章abstract::The aim of this study was to explore whether the cytotoxic T lymphocyte antigen-4 (CTLA-4) +49 A/G polymorphism confers susceptibility to rheumatoid arthritis (RA). A meta-analysis was conducted on the associations between CTLA-4 +49 A/G polymorphism and RA using; 1) allele contrast, 2) the recessive model, 3) the dom...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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pub_type: 杂志文章,meta分析
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pub_type: 杂志文章,meta分析
doi:10.1007/s11033-013-2503-9
更新日期:2013-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular biology reports
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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