Cloning and functional characterization of a caffeic acid O-methyltransferase from Trigonella foenum-graecum L.

abstract：:The Italian National Reference Center for Echinococcosis (CeNRE, Sassari, Italy) set up a diagnostic protocol of "one-step-PCR" useful for the detection of E. granulosus sensu stricto (E.g.s.s.) and the identification of its genotype (G1-G3). The purpose of this work was to perform the validation of the "PCR E.g.s.s."...

journal_title：Molecular biology reports

authors： Santucciu C,Masu G,Mura A,Peruzzu A,Piseddu T,Bonelli P,Masala G

更新日期：2019-04-01 00:00:00

abstract：:The oligochaete earthworm, Eudrilus eugeniae is capable of regenerating both anterior and posterior segments. The present study focuses on the transcriptome analysis of earthworm E. eugeniae to identify and functionally annotate the key genes supporting the anterior blastema formation and regulating the anterior regen...

journal_title：Molecular biology reports

authors： Paul S,Balakrishnan S,Arumugaperumal A,Lathakumari S,Syamala SS,Arumugaswami V,Sivasubramaniam S

更新日期：2020-12-11 00:00:00

abstract：:The full-length MECPS cDNA sequence (designated as Chmecps, GenBank Accession No.: DQ415658) was isolated by rapid amplification of cDNA ends (RACE) for the first time from Cephalotaxus harringtonia. The full-length cDNA of Chmecps was 1,146 bp containing a 753 bp open reading frame (ORF) encoding a polypeptide of 250...

journal_title：Molecular biology reports

authors： Chen J,Xiao Y,Di P,Yu X,Chen W,Zhang L

更新日期：2009-09-01 00:00:00

abstract：:Altered expression and methylation pattern of tumor suppressor and DNA repair genes, in particular involved in mismatch repair (MMR) pathway, frequently occur in primary colorectal (CRC) tumors. However, little is known about (epi)genetic changes of these genes in precancerous and early stages of CRC. The aim of this ...

journal_title：Molecular biology reports

authors： Polakova Vymetalkova V,Vannucci L,Korenkova V,Prochazka P,Slyskova J,Vodickova L,Rusnakova V,Bielik L,Burocziova M,Rossmann P,Vodicka P

更新日期：2013-10-01 00:00:00

abstract：:The product of the OTUB1 gene is a member of the OTU superfamily of predicted cysteine proteases and inhibits cytokine gene transcription via its interaction with a ubiquitin protease and E3 ubiquitin ligase. To further understand the functions of the porcine OTUB1 gene, the subcellular localization of porcine OTUB1 p...

journal_title：Molecular biology reports

authors： Shan TL,Tang ZL,Guo DZ,Yang SL,Mu YL,Ma YH,Guan WJ,Li K

更新日期：2009-07-01 00:00:00

abstract：:Development of a rapid and accurate quantification method for the detection of microRNAs (miRNAs) has been desired, in particular, when they are differently expressed in normal and pathological conditions. However, various methods for the quantification of small non-coding RNAs as well as miRNAs have been described. T...

journal_title：Molecular biology reports

authors： Mohammadi-Yeganeh S,Paryan M,Mirab Samiee S,Soleimani M,Arefian E,Azadmanesh K,Mostafavi E,Mahdian R,Karimipoor M

更新日期：2013-05-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disease, with multiple genetic and environmental factors involving in its etiology. Angiotensin converting enzyme (ACE) gene was reported to have important roles in the development and progression of SLE. In this study, a case-control study was carried out to investi...

journal_title：Molecular biology reports

authors： Gong AM,Li XY,Wang YQ,Yan HX,Xu ZX,Feng Z,Xie YQ,Yin DH,Yang SZ

更新日期：2012-10-01 00:00:00

abstract：:Nerve growth factor (NGF) is required for the differentiation and maintenance of sympathetic and sensory neurons. In the present study, the recombinant expression of human nerve growth factor beta (hNGF-β) gene in rabbit bone marrow mesenchymal stem cells (rMSCs) was undertaken. Recombinant vector containing hNGF-β wa...

journal_title：Molecular biology reports

authors： Fan BS,Lou JY

更新日期：2010-12-01 00:00:00

abstract：:45S ribosomal precursor RNA and large heterogeneous RNA molecules (greater than 45S) extracted from human leukemic cells were incubated in vitro with purified RNase III, which specifically attacks double-helical RNA regions. About 50% of the ribosomal precursor was cleaved into two major fragments sedimenting at 28S a...

journal_title：Molecular biology reports

authors： Torelli UL,Ferrari S,Torelli GM,Cadossi R,Ferrari S,Montagnani G,Narni F

更新日期：1977-12-01 00:00:00

abstract：:The binding of acridine orange and ethidium bromide to rat liver chromatin increases by 30% one hour after partial hepatectomy, returns to the control level by the second hour and increases again by the sixth hour. The changes described were found in investigations carried out on whole cells, on isolated nuclei, and o...

journal_title：Molecular biology reports

authors： Sondore OY,Fedoseeva GE,Kadyckov VA,Zelenin AV

更新日期：1978-10-16 00:00:00

abstract：:Control of polypeptide synthesis plays an important role in cell proliferation and translation rates generally reflect the growth state of the cultured eukaryotic cell. Physiological regulation of protein synthesis is almost always exerted at the level of polypeptide chain initiation, with the binding of mRNA to the s...

journal_title：Molecular biology reports

authors： Morley SJ

更新日期：1994-05-01 00:00:00

abstract：:Sulfonamide derivatives, such as sulfadiazine (SD) are used as antibiotics and, very recently, anti-amyloid properties of these have been reported. We have evaluated binding of SD with bovine serum albumin (BSA) followed by unfolding of protein. Studies were accomplished at physiological conditions of temperature (37 ...

journal_title：Molecular biology reports

authors： Al-Lohedan HA,Sajih Ali M

更新日期：2013-11-01 00:00:00

abstract：:Cartilage has a limited self-repair capability and the repair of large cartilage defects remains a challenge in clinic. This study aimed to investigate the effect of mesenchymal stem cells (MSCs) loaded three-dimensional bilayer collagen scaffold for cartilage repair. Cross-linked three-dimensional bilayer collagen sc...

journal_title：Molecular biology reports

authors： Qi Y,Zhao T,Xu K,Dai T,Yan W

更新日期：2012-02-01 00:00:00

abstract：:A novel 4-hydroxyphenylpyruvate dioxygenase gene (designated as Smhppd) was cloned from hairy roots of Salvia miltiorrhiza Bung. The full-length cDNA of Smhppd was 1,736 bp long with an ORF (open reading frame) that putatively encoded a polypeptide of 481 amino acids, with a predicted molecular mass of 52.54 kDa. The ...

journal_title：Molecular biology reports

authors： Xiao Y,Di P,Chen J,Liu Y,Chen W,Zhang L

更新日期：2009-09-01 00:00:00

abstract：:Chrysanthemum indicum L. is a traditional oriental medicinal herb prepared as a tea from flowers that have been used in China and South Korea since ancient times. It has a long history in the treatment of hypertension, inflammation, and respiratory diseases. Among Chrysanthemum species, C. indicum has more active chem...

journal_title：Molecular biology reports

authors： Abid S,Kaliraj L,Arif MH,Hurh J,Ahn JC,Yang DC,Jung SK

更新日期：2020-10-01 00:00:00

abstract：:Transgenic doubled haploid rapeseed (Brassica napus L. cvs. Global and PF(704)) plants were obtained from microspore-derived embryo (MDE) hypocotyls using the microprojectile bombardment. The binary vector pCAMBIA3301 containing the gus and bar genes under control of CaMV 35S promoter was used for bombardment experime...

journal_title：Molecular biology reports

authors： Abdollahi MR,Moieni A,Mousavi A,Salmanian AH

更新日期：2011-02-01 00:00:00

abstract：:Familial Mediterranean fever is an autosomal recessive disorder characterized by recurrent attacks of abdominal pain, synovitis and pleuritis. MEFV gene mutations are responsible for the disease. The objective of this study was to identify the frequency and distribution of 12 MEFV mutations in 153 Syrian patients and ...

journal_title：Molecular biology reports

authors： Jarjour RA

更新日期：2010-01-01 00:00:00

abstract：:Studies of the refractive properties of Gramicidin A in absolute ethanol and in ethanol water mixtures showed that this peptide in solution undergoes a conformational transition resulting in species with different refractivity. Accordingly, the folded form of this peptide shows a lower specific refractive index increm...

journal_title：Molecular biology reports

authors： Derechin M,Jordan BE

更新日期：1975-07-01 00:00:00

abstract：:The aim of this study was to explore whether the cytotoxic T lymphocyte antigen-4 (CTLA-4) +49 A/G polymorphism confers susceptibility to rheumatoid arthritis (RA). A meta-analysis was conducted on the associations between CTLA-4 +49 A/G polymorphism and RA using; 1) allele contrast, 2) the recessive model, 3) the dom...

journal_title：Molecular biology reports

authors： Lee YH,Bae SC,Choi SJ,Ji JD,Song GG

更新日期：2012-05-01 00:00:00

abstract：:The lily-of-the-valley Convallaria (Asparagaceae) consists of three herbaceous perennial species. The plants are commonly found in northern hemisphere, and are best-known for their ornamental and pharmaceutical value. In order to assess the genetic structure, diversity and demographic history of Convallaria species, 1...

journal_title：Molecular biology reports

authors： Lu QX,Gao J,Wu JJ,Zhou X,Wu X,Li MD,Wei YK,Wang RH,Qi ZC,Li P

更新日期：2020-04-01 00:00:00

abstract：:Osteoporosis is a common health problem in Mexico, so it is essential to investigate the status of different gene polymorphisms that could serve as genetic susceptibility markers in the Mexican population. Genes with a role in bone metabolism are excellent candidates for association studies. In this study were determi...

journal_title：Molecular biology reports

authors： Falcón-Ramírez E,Casas-Avila L,Cerda-Flores RM,Castro-Hernández C,Rubio-Lightbourn J,Velázquez-Cruz R,Diez-G P,Peñaloza-Espinosa R,Valdés-Flores M

更新日期：2013-03-01 00:00:00

abstract：:Allelic variation of BAT-25 (a 25-repeat quasimonomorphic poly T) and BAT-26 (a 26-repeat quasimonomorphic polyA) loci as two mononucleotide microsatellite markers, were analyzed with high-performance liquid chromatography (HPLC) compared with Real-Time PCR using hybridization probes. BAT-26 and BAT-25 markers were us...

journal_title：Molecular biology reports

authors： Mokarram P,Rismanchi M,Alizadeh Naeeni M,Mirab Samiee S,Paryan M,Alipour A,Honardar Z,Kavousipour S,Naghibalhossaini F,Mostafavi-Pour Z,Monabati A,Hosseni SV,Shamsdin SA

更新日期：2014-05-01 00:00:00

abstract：AIM:To study the function of the prodomain of ADAM17 (TACE) and to develop an approach for interfering with inflammation processes. METHOD:The expression plasmids of the TACE ectodomain (T1300), prodomain (T591), signal peptide and prodomain (T648), full length (T2472), and the turncated TACE without prodomain (T57-T1...

journal_title：Molecular biology reports

authors： Li X,Yan Y,Huang W,Yang Y,Wang H,Chang L

更新日期：2009-04-01 00:00:00

abstract：:Current customers' demands focus on the nutritional and sensory quality of cattle meat. Candidate gene approach allows identification of genetic polymorphisms that have a measurable effect on traits of interest. The aim of this work is to identify new molecular markers for beef production through an association study ...

journal_title：Molecular biology reports

authors： Sevane N,Armstrong E,Wiener P,Pong Wong R,Dunner S,GemQual Consortium.

更新日期：2014-07-01 00:00:00

abstract：:1-aminocyclopropane-1-carboxylic acid oxidase (ACO) enzyme is a member of the Fe II-dependent family of oxidases/oxygenases which require Fe(2+) as a cofactor, ascorbate as a cosubstrate and CO(2) as an activator. This enzyme catalyses the terminal step in the plant signaling of ethylene biosynthetic pathway. A 948 bp...

journal_title：Molecular biology reports

authors： Jafari Z,Haddad R,Hosseini R,Garoosi G

更新日期：2013-02-01 00:00:00

abstract：:The purpose of this study was to investigate the efficacy of Endostar combined with chemotherapy on human esophageal squamous cell carcinoma Eca-109 in mice. The tumor xenograft models were established and randomly assigned to 4 groups: control group, Endostar group (1.5 mg/kg day, once daily for 3 weeks), chemotherap...

journal_title：Molecular biology reports

authors： Chang L,Guo F,Lv Y,Wang Y,Huo B,Wang L,Liu W

更新日期：2013-01-01 00:00:00

abstract：:Replication of the influenza virus strains Influenza Ao/WSN (H0N1), fowl plague (Hav1N1) and B-Lee/40 (ATCC) and the paramyxovirus, New Castle disease virus (Victoria) are highly sensitive to human interferon type alpha in Madin Darby bovine kidney cells. Pretreatment of cells with human interferon type alpha resulted...

journal_title：Molecular biology reports

authors： Strube M,Bodo G,Jungwirth C

更新日期：1985-10-01 00:00:00

abstract：:Hypertension is a risk factor for the cardiovascular diseases. Although, several drugs are used to treat hypertension, the success of the antihypertensive therapy is limited. Resveratrol decreases blood pressure in animal models of hypertension. This study researched the mechanisms behind the effects of resveratrol on...

journal_title：Molecular biology reports

authors： Han S,Uludag MO,Usanmaz SE,Ayaloglu-Butun F,Akcali KC,Demirel-Yilmaz E

更新日期：2015-01-01 00:00:00

abstract：:Relying on the regulation of transcription factors, plants resist to various abiotic and biotic stresses. NAC (NAM, ATAF1/2, CUC2) are one of the largest families of plant-specific transcription factors and known to play important roles in plant development and response to environmental stresses. A new NAC gene was cl...

journal_title：Molecular biology reports

authors： Wang YX

更新日期：2013-11-01 00:00:00

abstract：:Members of the methyl binding domain (MBD) protein family are known for binding to methylated DNA by recognizing methylated cytosines. Their original function is to regulate protein biosynthesis by recruitment of transcriptional repression complexes to silence gene expression. The aim of the presented work was to dete...

journal_title：Molecular biology reports

authors： Födermayr M,Pröll J,Zach O,Wechselberger C,Lutz D

更新日期：2009-09-01 00:00:00