Abstract:
:Members of the methyl binding domain (MBD) protein family are known for binding to methylated DNA by recognizing methylated cytosines. Their original function is to regulate protein biosynthesis by recruitment of transcriptional repression complexes to silence gene expression. The aim of the presented work was to detect methylated DNA spotted onto nitrocellulose membranes with recombinant proteins MBD2b, MBD2b-GFP and directly labeled protein MBD2b. Proteins were affinity purified and tested for functionality before application. We were able to show that these functional recombinant proteins bind to unilaterally and symmetrically methylated oligonucleotides and genomic DNA in vitro and thus can be used in various detection assays.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Födermayr M,Pröll J,Zach O,Wechselberger C,Lutz Ddoi
10.1007/s11033-008-9391-4subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1859-62issue
7eissn
0301-4851issn
1573-4978journal_volume
36pub_type
杂志文章abstract::Recent evidence supports a role of Toll-like receptor (TLR) signaling in the development of atherosclerotic lesions. It was confirmed that the presence of functional TLR4 promotes a proinflammatory phenotype and proliferation of vascular smooth muscle cells (VSMCs). Here we tested whether designed TLR4 small interferi...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0246-4
更新日期:2011-02-01 00:00:00
abstract::The MinION is a miniaturized high-throughput next generation sequencing platform of novel conception. The use of nucleic acids derived from formalin-fixed paraffin-embedded samples is highly desirable, but their adoption for molecular assays is hurdled by the high degree of fragmentation and by the chemical-induced mu...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-017-4133-0
更新日期:2018-02-01 00:00:00
abstract::The purpose of this research is to identify the probable mitochondrial factor associated with cytoplasmic male sterility (cms) by comparative analysis of cms and its isogenic maintainer lines in stem mustards. Dramatic variations in the morphology of floral organs were observed in cms stem mustard. Mitochondrial atpA ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-007-9176-1
更新日期:2009-02-01 00:00:00
abstract::We reported that an aqueous extract of Portulaca oleracea L. inhibited high-fat-diet-induced oxidative injury in a dose-dependent manner. Male kunming mice (5-weeks-old, 24 g) were used in this experiment. After a 4-day adaptation period, animals were randomly divided into four groups (n = 10 in each group); Group 1: ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1644-6
更新日期:2012-08-01 00:00:00
abstract::As-ClC (chloride channels protein from Artemia sinica), a member from the chloride channels protein family, is a α-helical membrane protein predicted to traverse the cell membrane 11 times. It is important for several physiological functions such as cell volume regulation, cell proliferation, growth and differentiatio...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2441-y
更新日期:2013-05-01 00:00:00
abstract::Antimicrobial resistance is increasing around the world and the search for effective treatment options, such as new antibiotics and combination therapy is urgently needed. The present study evaluates oregano essential oil (OEO) antibacterial activities against reference and multidrug-resistant clinical isolates of Aci...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-020-05989-0
更新日期:2020-12-01 00:00:00
abstract::Disorders in the proliferation and apoptosis of thyrocytes may induce goitre, adenoma and carcinoma in the thyroid. The Wnt/beta-catenin pathway has been demonstrated to be involved in the regulation of cell proliferation, differentiation and apoptosis in various cell lines. The regulatory mechanism on the proliferati...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9819-5
更新日期:2010-07-01 00:00:00
abstract::The Fas gene polymorphisms -670A/G (rs1800682) and -1377G/A (rs2234767) have been shown to be associated with systemic lupus erythematosus (SLE), but findings are not consistent. To clarify this point, a meta-analysis was performed. We searched PubMed, CNKI, CBM and Wanfang database. Meta-odds ratios (ORs) and 95 % co...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-2075-0
更新日期:2013-01-01 00:00:00
abstract::Micro-RNAs (miRNAs) play key roles in regulating genes of the immune system. The aim of this study was to examine the expression of miR-1, 21 and 125a in the immune cells taken from the peripheral blood of patients suffering from chronic HBV infection (CHB). This cross-sectional study was performed on 60 CHB patients ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3355-7
更新日期:2014-07-01 00:00:00
abstract::70S RNA has been prepared from mouse mammary tumor virus (MMTV) produced by the GR tumor cell line. After denaturation for 3 min at 60 degrees C the RNA was applied to a sucrose gradient and molecules sedimenting between 10-16S were selected and passed over an oligo-dT cellulose column. The poly A containing RNA fract...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/BF00778742
更新日期:1981-05-22 00:00:00
abstract::Se is an essential trace element and is found as a selenocysteine in the active site of Se-enzymes, such as glutathione peroxidase. tRNASec is first aminoacylated with serine by Ser RS and further is converted to selenocysteyl-tRNA by selenocysteine synthase. Mammalian selenocysteine tRNA has dual identities with Ser ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1023/a:1006879820805
更新日期:1998-11-01 00:00:00
abstract::To determine the plasma lipid and lipoprotein profiles and their possible association with the type of β-thalassemia mutation we studied 103 major β-thalassemia patients including 71 children and 32 young adults compared to 102 healthy subjects consisted of 90 children and 12 young healthy adults. The plasma lipid and...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0397-3
更新日期:2011-04-01 00:00:00
abstract::The investigation was carried out to analyse the genetic polymorphism and gene expression of ATP1A1 gene in four different Indian goat breeds by using high resolution melting (HRM) and real time-PCR. ATPase is electro-genic ion pump which is maintains the balance of sodium and potassium ions in animal cells. The trans...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04984-4
更新日期:2019-10-01 00:00:00
abstract::Dendropoma petraeum, considered the primary vermetid reef-building species in the Mediterranean, has recently been shown to be a species complex of at least four cryptic species. These species have highly restricted, non-overlapping distributions, causing concern for their conservation status. To better study the gene...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4346-x
更新日期:2018-12-01 00:00:00
abstract::Recently genetics and epigenetics alterations have been found to be characteristic of malignancy and hence can be used as targets for detection of neoplasia. RAS association domain family protein 1A (RASSF1A) gene hypermethylation has been a subject of interest in recent researches on cancer breast patients. The aim o...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2837-3
更新日期:2014-01-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a chronic inflammatory disease in which numerous cells and mediators affect inflammatory conditions and disease severity. To compare the serum levels of adiponectin, vitamin D, copper, and zinc in patients with RA and to investigate the relationship between these parameters and RA severity...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-019-04682-1
更新日期:2019-04-01 00:00:00
abstract::Birch (Betula platyphylla), an eminent tree species in Northeast and Inner Mongolia of China, has been widely used in architecture, furniture, and paper making in recent years. In order to retrieve genes involved in early development of B. platyphylla male inflorescence, RNA populations extracted from early and late d...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0818-y
更新日期:2012-02-01 00:00:00
abstract::Bud dormancy in perennial plants adapts to environmental and seasonal changes. Bud dormancy is of ecological interest because it affects forest population growth characteristics and is of economical interest because it impacts wood production levels. To understand Pinus sylvestris L. var. mongolica litv. bud-dormancy ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-010-0159-2
更新日期:2011-02-01 00:00:00
abstract::The Follicle stimulating hormone beta polypeptide (FSHβ) gene has been investigated as a candidate gene for litter size because of its role in maturation of small and medium follicles into ovulating large follicles. Genes coding follicle stimulating hormone have been found to have significant effects on litter size in...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-014-3789-y
更新日期:2015-02-01 00:00:00
abstract::ERBB2/HER2/NEU, a member of the epidermal growth factor receptor family, is overexpressed in more than 25 % of non-small cell lung cancer and is considered to be a significant and independent prognostic factor in lung cancer. Here we generated a lung specific HER2 overexpressing transgenic mouse model. In this model, ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-1635-7
更新日期:2012-08-01 00:00:00
abstract::The human dopamine transporter gene (hDAT) encodes the dopamine transporter in dopamine (DA) neurons to regulate DA transmission. hDAT expression varies significantly from neuron to neuron, and from individual to individual so that dysregulation of hDAT is related to many neuropsychiatric disorders. It is critical to ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-1339-4
更新日期:2012-05-01 00:00:00
abstract::The rapid progress made over the last 10 years in the identification of individual autoantigens and in the localization of the epitopes involved, has resulted in a parallel reduction in the complexity of the antigen required for the detection of autoantibodies. The ability to use synthetic peptides as antigens is a re...
journal_title:Molecular biology reports
pub_type: 杂志文章,评审
doi:10.1007/BF00464709
更新日期:1992-06-01 00:00:00
abstract::Staudtia kamerunensis (Myristicaceae) or 'Niové' is an evergreen tree widespread in Central African moist forests. The bark and seeds are used in traditional medicine, yet the tree is mainly harvested for its high quality, multi-purpose timber. To facilitate sustainable harvesting and conservation of the species, we a...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-018-4239-z
更新日期:2018-10-01 00:00:00
abstract::The aim of this work was to study β-defensin 1 (SBD1) and β-defensin 2 (SBD2) genes in Valle del Belice dairy sheep in order to identify polymorphisms that can be utilized as markers of the analyzed genes, and search for the functional effects and roles of the identified polymorphisms (variation of the amino acid sequ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-011-0694-5
更新日期:2011-11-01 00:00:00
abstract::Body measurement and meat quality traits which play important roles in the assessment of productivity and economy in cattle were influenced by genes and environmental factors. Latest studies showed that LYR motif containing 1 (LYRM1) may be involved in influencing fatness deposition in animals. The objective of this s...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2195-6
更新日期:2013-02-01 00:00:00
abstract::The full length cDNA sequence of the Tpt1/TCTP (Tumor protein, Translationally-controlled1) gene was identified from Common Carp (Cyprinus carpio, Cyprinidae), and was designated as CcTpt1 gene. The CDS is 510 bp and encodes a 170-amino acid peptide with a typical Tpt1 signature 2 domain, and is a typical Tpt1 protein...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-013-2806-x
更新日期:2013-10-13 00:00:00
abstract::Cytochrome P450 1B1 (CYP1B1) is a key P450 enzyme involved in the metabolism of exogenous and endogenous substrates in endocrine-mediated tumors such as prostate cancer. The potential significance of nonsynonymous SNP Leu432Val (rs1056836) as a risk factor in prostate cancer has been extensively studied. The objective...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-012-1579-y
更新日期:2012-07-01 00:00:00
abstract::Molecular abnormalities in the 11p15.5 imprinted gene cluster lead to two different growth diseases: Beckwith-Wiedemann syndrome (BWS) and Silver-Russell syndrome (SRS). They are mainly caused by epigenetic alterations in one of the two imprinting 11p15 control regions (ICR1 and ICR2). These CpG-rich regions are diffe...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-012-2057-2
更新日期:2013-01-01 00:00:00
abstract::Several studies have examined the effects of the tumor necrosis factor-α (TNF-α) -308 A/G and lymphotoxin-α (LT-α) +252 A/G polymorphisms on susceptibility to sarcoidosis, showing mixed results. The purpose of this study was to examine whether the TNF-α -308 A/G and LT-α +252 A/G polymorphisms confer susceptibility to...
journal_title:Molecular biology reports
pub_type: 杂志文章,meta分析
doi:10.1007/s11033-013-2859-x
更新日期:2014-01-01 00:00:00
abstract::In higher eukaryotes mechanism of DNA replication origin recognition and binding by origin recognition complex (ORC) is still unknown. Origin transfer studies have shown that origin sites are genetically determined, containing functionally interchangeable modules. One of such modules from the human lamin B2 origin of ...
journal_title:Molecular biology reports
pub_type: 杂志文章
doi:10.1007/s11033-009-9735-8
更新日期:2010-06-01 00:00:00